DOI: 10.1017/s1551929500054341. Automatic Acquisition and Image Analysis of 2D Crystals

Automatic Acquisition and Image Analysis of 2D Crystals

10.1017/s1551929500054341

Crossref journal-article

Oxford University Press (OUP)

Microscopy Today (286)

Abstract

Abstract 2D-crystallization in combination with transmission electron microscopy (TEM) is one of the few methods for the structural analysis of membrane proteins in their native state. However, the parameters for the generation of large crystalline sheets are typically difficult to identify for a given protein. Many repetitive and time consuming screening steps by TEM are therefore necessary to find the best crystallization and preparation conditions. Although several software packages offer the possibility to control an electron microscope, none is completely adapted for a fully automated and completely integrated acquisition and analysis of 2D crystals. Here we report on the development of a fully automatic screening and on-line analysis software for the fast and automatic survey of large quantities of negatively stained EM samples for 2D crystallography.

Bibliography

Coudray, N., Beck, F., Buessler, J.-L., Korinek, A., Karathanou, A., RÃ©migy, H., Kihl, H., Engel, A., Plitzko, J., & Urban, J.-P. (2008). Automatic Acquisition and Image Analysis of 2D Crystals. Microscopy Today, 16(1), 48â49.

Authors 10

N Coudray (first)
F Beck (additional)
J-L Buessler (additional)
A Korinek (additional)
A Karathanou (additional)
H Rémigy (additional)
H Kihl (additional)
A Engel (additional)
JM Plitzko (additional)
J-P Urban (additional)

References 6 Referenced 7

10.1016/S0014-5793(03)01105-0 / FEBS Letters by Rémigy (2003)
10.1016/S0968-4328(00)00049-4 / Micron by Ellis (2001)
10.1016/j.jsb.2004.10.006 / Journal of Structural Biology by Nickell (2005)
{'key': '2023010802374222700_ref004', 'volume-title': 'Physics in Signal and Image Processing (PSIP),', 'author': 'Coudray', 'year': '2007'} / Physics in Signal and Image Processing (PSIP), by Coudray (2007)
10.1016/S0014-5793(03)00082-6 / FEBS Letters by Karlsson (2003)
{'key': '2023010802374222700_ref006'}

Dates

Type	When
Created	7 years, 3 months ago (May 4, 2018, 8:36 a.m.)
Deposited	2 years, 6 months ago (Feb. 14, 2023, 4:13 p.m.)
Indexed	1 year, 3 months ago (May 1, 2024, 6:19 a.m.)
Issued	17 years, 7 months ago (Jan. 1, 2008)
Published	17 years, 7 months ago (Jan. 1, 2008)
Published Online	7 years, 5 months ago (March 14, 2018)
Published Print	17 years, 7 months ago (Jan. 1, 2008)

Funders 0

None

BibTeX

@article{Coudray_2008, title={Automatic Acquisition and Image Analysis of 2D Crystals}, volume={16}, ISSN={1551-9295}, url={http://dx.doi.org/10.1017/s1551929500054341}, DOI={10.1017/s1551929500054341}, number={1}, journal={Microscopy Today}, publisher={Oxford University Press (OUP)}, author={Coudray, N and Beck, F and Buessler, J-L and Korinek, A and Karathanou, A and Rémigy, H and Kihl, H and Engel, A and Plitzko, JM and Urban, J-P}, year={2008}, month=jan, pages={48–49} }

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