DOI: 10.1017/s1431927613013901. In-Focus Electrostatic Zach Phase Plate Imaging for Transmission Electron Microscopy with Tunable Phase Contrast of Frozen Hydrated Biological Samples

In-Focus Electrostatic Zach Phase Plate Imaging for Transmission Electron Microscopy with Tunable Phase Contrast of Frozen Hydrated Biological Samples

10.1017/s1431927613013901

Crossref journal-article

Oxford University Press (OUP)

Microscopy and Microanalysis (286)

Abstract

AbstractTransmission electron microscopy (TEM) images of beam sensitive weak-phase objects such as biological cryo samples usually show a very low signal-to-noise ratio. These samples have almost no amplitude contrast and instead structural information is mainly encoded in the phase contrast. To increase the sample contrast in the image, especially for low spatial frequencies, the use of phase plates for close to focus phase contrast enhancement in TEM has long been discussed. Electrostatic phase plates are favorable in particular, as their tunable potential will allow an optimal phase shift adjustment and higher resolution than film phase plates as they avoid additional scattering events in matter. Here we show the first realization of close to focus phase contrast images of actin filament cryo samples acquired using an electrostatic Zach phase plate. Both positive and negative phase contrast is shown, which is obtained by applying appropriate potentials to the phase plate. The dependence of phase contrast improvement on sample orientation with respect to the phase plate is demonstrated and single-sideband artifacts are discussed. Additionally, possibilities to reduce contamination and charging effects of the phase plate are shown.

Bibliography

Frindt, N., Oster, M., Hettler, S., Gamm, B., Dieterle, L., Kowalsky, W., Gerthsen, D., & SchrÃ¶der, R. R. (2014). In-Focus Electrostatic Zach Phase Plate Imaging for Transmission Electron Microscopy with Tunable Phase Contrast of Frozen Hydrated Biological Samples. Microscopy and Microanalysis, 20(1), 175â183.

Authors 8

Nicole Frindt (first)
Marco Oster (additional)
Simon Hettler (additional)
Björn Gamm (additional)
Levin Dieterle (additional)
Wolfgang Kowalsky (additional)
Dagmar Gerthsen (additional)
Rasmus R. Schröder (additional)

References 25 Referenced 18

10.1016/S0039-6028(98)00363-X
10.1016/j.ultramic.2006.09.001
10.1016/j.ultramic.2011.04.004
10.1007/s00249-008-0264-5
{'key': 'S1431927613013901_ref001', 'volume-title': 'Molecular Biology of the Cell', 'author': 'Alberts', 'year': '2008'} / Molecular Biology of the Cell by Alberts (2008)
10.1515/zna-1947-11-1204 / Z Naturforschung / Über die Kontraste von Atomen im Elektronenmikroskop by Boersch (1947)
10.1038/347044a0
10.1016/0304-3991(96)00033-2
10.1016/j.ultramic.2011.09.015
10.1016/S0304-3991(01)00088-2
10.1016/0921-4526(95)00630-3
10.1017/S1431927610055042
Zach J. (2008). Phase plate, image producing method and electron microscope. PCT Patent WO/2008/061603; EPO Patent 1989723-A2, European Patent Office, Munich.
10.1023/A:1021234621466
10.1111/j.1365-2818.1982.tb04625.x
Frindt N. (2013). Development and implementation of electrostatic Zach phase plates for phase contrast transmission electron microscopy. PhD thesis. Universität Heidelberg, Heidelberg, Germany.
10.1016/j.ultramic.2010.02.006
10.1016/j.ultramic.2013.05.023
10.1016/j.ultramic.2006.07.006
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{'key': 'S1431927613013901_ref019', 'volume-title': 'Transmission Electron Microscopy: Physics of Image Formation', 'author': 'Reimer', 'year': '2008'} / Transmission Electron Microscopy: Physics of Image Formation by Reimer (2008)
10.1063/1.3353991
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10.1017/S1431927612001560

Dates

Type	When
Created	11 years, 7 months ago (Jan. 2, 2014, 6:18 a.m.)
Deposited	2 years, 6 months ago (Feb. 14, 2023, 3:38 p.m.)
Indexed	1 year, 6 months ago (Feb. 24, 2024, 11:47 a.m.)
Issued	11 years, 7 months ago (Jan. 2, 2014)
Published	11 years, 7 months ago (Jan. 2, 2014)
Published Online	11 years, 7 months ago (Jan. 2, 2014)
Published Print	11 years, 6 months ago (Feb. 1, 2014)

Funders 0

None

BibTeX

@article{Frindt_2014, title={In-Focus Electrostatic Zach Phase Plate Imaging for Transmission Electron Microscopy with Tunable Phase Contrast of Frozen Hydrated Biological Samples}, volume={20}, ISSN={1435-8115}, url={http://dx.doi.org/10.1017/s1431927613013901}, DOI={10.1017/s1431927613013901}, number={1}, journal={Microscopy and Microanalysis}, publisher={Oxford University Press (OUP)}, author={Frindt, Nicole and Oster, Marco and Hettler, Simon and Gamm, Björn and Dieterle, Levin and Kowalsky, Wolfgang and Gerthsen, Dagmar and Schröder, Rasmus R.}, year={2014}, month=jan, pages={175–183} }

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