DOI: 10.1017/s1431927608080781. An Improved Cryogen for Plunge Freezing

10.1017/s1431927608080781

Crossref journal-article

Oxford University Press (OUP)

Microscopy and Microanalysis (286)

Abstract

AbstractThe use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.

Bibliography

Tivol, W. F., Briegel, A., & Jensen, G. J. (2008). An Improved Cryogen for Plunge Freezing. Microscopy and Microanalysis, 14(5), 375â379.

Authors 3

William F. Tivol (first)
Ariane Briegel (additional)
Grant J. Jensen (additional)

References 3 Referenced 298

Dates

Type	When
Created	16 years, 11 months ago (Sept. 16, 2008, 6:10 a.m.)
Deposited	2 years, 6 months ago (Feb. 14, 2023, 3:52 p.m.)
Indexed	4 weeks ago (July 24, 2025, 7:14 a.m.)
Issued	16 years, 11 months ago (Sept. 16, 2008)
Published	16 years, 11 months ago (Sept. 16, 2008)
Published Online	16 years, 11 months ago (Sept. 16, 2008)
Published Print	16 years, 10 months ago (Oct. 1, 2008)

Funders 0

None

BibTeX

@article{Tivol_2008, title={An Improved Cryogen for Plunge Freezing}, volume={14}, ISSN={1435-8115}, url={http://dx.doi.org/10.1017/s1431927608080781}, DOI={10.1017/s1431927608080781}, number={5}, journal={Microscopy and Microanalysis}, publisher={Oxford University Press (OUP)}, author={Tivol, William F. and Briegel, Ariane and Jensen, Grant J.}, year={2008}, month=sep, pages={375–379} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        24
      ]
    ],
    "date-time": "2025-07-24T11:14:59Z",
    "timestamp": 1753355699290
  },
  "reference-count": 3,
  "publisher": "Oxford University Press (OUP)",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            9,
            16
          ]
        ],
        "date-time": "2008-09-16T00:00:00Z",
        "timestamp": 1221523200000
      },
      "content-version": "unspecified",
      "delay-in-days": 0,
      "URL": "https://www.cambridge.org/core/terms"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2008,
        10
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.</jats:p>",
  "DOI": "10.1017/s1431927608080781",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2008,
        9,
        16
      ]
    ],
    "date-time": "2008-09-16T10:10:49Z",
    "timestamp": 1221559849000
  },
  "page": "375-379",
  "source": "Crossref",
  "is-referenced-by-count": 298,
  "title": "An Improved Cryogen for Plunge Freezing",
  "prefix": "10.1093",
  "volume": "14",
  "author": [
    {
      "given": "William F.",
      "family": "Tivol",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Ariane",
      "family": "Briegel",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Grant J.",
      "family": "Jensen",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        2008,
        9,
        16
      ]
    ]
  },
  "reference": [
    {
      "key": "S1431927608080781_ref003",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1128245"
    },
    {
      "key": "S1431927608080781_ref002",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/288569a0"
    },
    {
      "key": "S1431927608080781_ref001",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2008.06219.x"
    }
  ],
  "container-title": "Microscopy and Microanalysis",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1431927608080781",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        2,
        14
      ]
    ],
    "date-time": "2023-02-14T20:52:58Z",
    "timestamp": 1676407978000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/mam/article/14/5/375/6919956"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2008,
        9,
        16
      ]
    ]
  },
  "references-count": 3,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          2008,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "S1431927608080781"
  ],
  "URL": "http://dx.doi.org/10.1017/S1431927608080781",
  "relation": {},
  "ISSN": [
    "1431-9276",
    "1435-8115"
  ],
  "subject": [],
  "container-title-short": "Microsc Microanal",
  "published": {
    "date-parts": [
      [
        2008,
        9,
        16
      ]
    ]
  }
}