New Approaches to the Microscopic Imaging ofTrypanosoma brucei
10.1017/s1431927604040942
Crossref journal-article
Oxford University Press (OUP)
Microscopy and Microanalysis (286)
Abstract

Protozoan parasites are fearsome pathogens responsible for a substantial proportion of human mortality, morbidity, and economic hardship. The principal disease agents are members of the orders Apicomplexa (Plasmodium, Toxoplasma, Eimeria) and Kinetoplastida (Trypanosomes, Leishmania). The majority of humans are at risk from infection from one or more of these organisms, with profound effects on the economy, social structure and quality of life in endemic areas; Plasmodium itself accounts for over one million deaths per annum, and an estimated 4 × 107disability-adjusted life years (DALYs), whereas the Kinetoplastida are responsible for over 100,000 deaths per annum and 4 × 106DALYs. Current control strategies are failing due to drug resistance and inadequate implementation of existing public health strategies.Trypanosoma brucei, the African Trypanosome, has emerged as a favored model system for the study of basic cell biology in Kinetoplastida, because of several recent technical advances (transfection, inducible expression systems, and RNA interference), and these advantages, together with genome sequencing efforts are widely anticipated to provide new strategies of therapeutic intervention. Here we describe a suite of methods that have been developed for the microscopic analysis ofT. bruceiat the light and ultrastructural levels, an essential component of analysis of gene function and hence identification of therapeutic targets.

Bibliography

Field, M. C., Allen, C. L., Dhir, V., Goulding, D., Hall, B. S., Morgan, G. W., Veazey, P., & Engstler, M. (2004). New Approaches to the Microscopic Imaging ofTrypanosoma brucei. Microscopy and Microanalysis, 10(5), 621–636.

Authors 8
  1. Mark C. Field (first)
  2. Clare L. Allen (additional)
  3. Vivek Dhir (additional)
  4. David Goulding (additional)
  5. Belinda S. Hall (additional)
  6. Gareth W. Morgan (additional)
  7. Paul Veazey (additional)
  8. Markus Engstler (additional)
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Dates
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Created 20 years, 9 months ago (Nov. 3, 2004, 11:01 a.m.)
Deposited 2 years, 3 months ago (April 29, 2023, 6:15 p.m.)
Indexed 2 months ago (June 23, 2025, 8:26 a.m.)
Issued 20 years, 10 months ago (Oct. 1, 2004)
Published 20 years, 10 months ago (Oct. 1, 2004)
Published Online 20 years, 10 months ago (Oct. 1, 2004)
Published Print 20 years, 10 months ago (Oct. 1, 2004)
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@article{Field_2004, title={New Approaches to the Microscopic Imaging ofTrypanosoma brucei}, volume={10}, ISSN={1435-8115}, url={http://dx.doi.org/10.1017/s1431927604040942}, DOI={10.1017/s1431927604040942}, number={5}, journal={Microscopy and Microanalysis}, publisher={Oxford University Press (OUP)}, author={Field, Mark C. and Allen, Clare L. and Dhir, Vivek and Goulding, David and Hall, Belinda S. and Morgan, Gareth W. and Veazey, Paul and Engstler, Markus}, year={2004}, month=oct, pages={621–636} }