DOI: 10.1017/s1431927600026544. Cryo-Electron Tomography of Isolated Triad Junctions from Skeletal Muscle

Cryo-Electron Tomography of Isolated Triad Junctions from Skeletal Muscle

10.1017/s1431927600026544

Crossref journal-article

Oxford University Press (OUP)

Microscopy and Microanalysis (286)

Abstract

Abstract In skeletal muscle, depolarization of the plasma membrane, which is initiated at the neuromuscular junction, is transduced to a rise in cytoplasmic calcium at specialized structures known as triad junctions (TJs). TJs occur in the myofiber’s interior at regions near the z-lines, where transversely oriented tubular invaginations of the plasma membrane (T-tubules) form junctions with two elements of the sarcoplasmic reticulum (SR). Isolation of membrane fractions that are enriched in junctional complexes and which retain function has been reported. Figure 1 shows a region of an electron micrograph containing an isolated TJ in the frozen-hydrated state. in the orientation shown, two SR-derived vesicles sandwich a flattened vesicle derived from the T-tubule. The junctional regions contain a complex distribution of density, presumably due to proteins that are known to be present in TJs. Electron tomography offers the means to determine the three-dimensional mass density from such micrographs, which would greatly aid in their interpretation. Only recently has the automated data collection technology for determining tomograms of non-stained, frozen-hydrated specimens become available. Here we describe the first tomographic reconstruction of a frozen-hydrated triad junction by automated electron tomography.

Bibliography

Hsieh, C.-E., Marko, M., Rath, B., Fleischer, S., & Wagenknecht, T. (2001). Cryo-Electron Tomography of Isolated Triad Junctions from Skeletal Muscle. Microscopy and Microanalysis, 7(S2), 94â95.

Authors 5

C-E Hsieh (first)
M Marko (additional)
BK Rath (additional)
S Fleischer (additional)
T Wagenknecht (additional)

References 5 Referenced 5

10.1016/0076-6879(88)57096-9 / Method. Enzymol. by Ikemoto (1988)
10.1016/S0962-8924(98)01423-8 / Trends in Cell Biology by Baumeister (1999)
10.1146/annurev.ph.56.030194.002453 / Ann. Rev. Physiol. by Franzini-Armstrong (1994)
10.1006/jsbi.1997.3924 / J. Struct. Biol. by Rath (1997)
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Dates

Type	When
Created	5 years, 1 month ago (July 2, 2020, 4:36 a.m.)
Deposited	11 months, 1 week ago (Sept. 9, 2024, 3:01 p.m.)
Indexed	11 months, 1 week ago (Sept. 9, 2024, 4:10 p.m.)
Issued	24 years ago (Aug. 1, 2001)
Published	24 years ago (Aug. 1, 2001)
Published Online	24 years ago (Aug. 1, 2001)
Published Print	24 years ago (Aug. 1, 2001)

Funders 0

None

BibTeX

@article{Hsieh_2001, title={Cryo-Electron Tomography of Isolated Triad Junctions from Skeletal Muscle}, volume={7}, ISSN={1431-9276}, url={http://dx.doi.org/10.1017/s1431927600026544}, DOI={10.1017/s1431927600026544}, number={S2}, journal={Microscopy and Microanalysis}, publisher={Oxford University Press (OUP)}, author={Hsieh, C-E and Marko, M and Rath, BK and Fleischer, S and Wagenknecht, T}, year={2001}, month=aug, pages={94–95} }

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