DOI: 10.1017/s1431927600025113. Correlative Instrumental Neutron Activation Analysis, LM, And TEM to Track in Vivo Distribution of Colloidal Gold Spheres in BALB/C Mice

Correlative Instrumental Neutron Activation Analysis, LM, And TEM to Track in Vivo Distribution of Colloidal Gold Spheres in BALB/C Mice

10.1017/s1431927600025113

Crossref journal-article

Oxford University Press (OUP)

Microscopy and Microanalysis (286)

Abstract

Abstract Colloidal gold is routinely used conjugated to ligands or antibodies as a label for the detection of cell structures by transmission (TEM) and scanning (SEM) electron microscopy. Silver enhancement, a procedure that amplifies the size of gold spheres, is used for light microscopic (LM) observation of colloidal gold. These techniques are valuable in localization studies but it is difficult to quantitate the level of labeling in larger volumes of tissue solely by microscopic means. Instrumental Neutron Activation Analysis (INAA) can be used to provide a quantitative index of gold accumulation at the organ and tissue levels. Given that gold is not naturally found in the body in any substantial amount and that colloidal gold and ligand conjugates can be prepared to yield uniform bead sizes, the amount of label can be determined at the organ and tissue levels in whole animal systems.

Bibliography

Hillyer, J., & Albrecht, R. (1998). Correlative Instrumental Neutron Activation Analysis, LM, And TEM to Track in Vivo Distribution of Colloidal Gold Spheres in BALB/C Mice. Microscopy and Microanalysis, 4(S2), 998â999.

Authors 2

JF Hillyer (first)
RM Albrecht (additional)

References 5 Referenced 2

This research was funded in part by the advanced opportunity fellowship program, University of Wisconsin - Madison. The aid of Paul Sims, Richard Cashwell, and Randall Massey is greatly appreciated.
{'key': 'S1431927600025113_ref003', 'first-page': '773', 'volume': '9', 'author': 'Darien', 'year': '1995', 'journal-title': 'Scanning Microsc.'} / Scanning Microsc. by Darien (1995)
Keisch, B. , The atomic fingerprint: neutron activation analysis, United States Atomic Energy Commission, Technical Information Center (1972).
10.1007/BF02473198
{'key': 'S1431927600025113_ref002', 'first-page': '151', 'volume-title': 'Immunocytochemistry: a practical approach', 'author': 'Albrecht', 'year': '1993'} / Immunocytochemistry: a practical approach by Albrecht (1993)

Dates

Type	When
Created	5 years, 1 month ago (July 6, 2020, 1:33 a.m.)
Deposited	11 months, 2 weeks ago (Sept. 9, 2024, 1:29 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 9, 2024, 8:07 p.m.)
Issued	27 years, 1 month ago (July 1, 1998)
Published	27 years, 1 month ago (July 1, 1998)
Published Online	27 years, 1 month ago (July 1, 1998)
Published Print	27 years, 1 month ago (July 1, 1998)

Funders 0

None

BibTeX

@article{Hillyer_1998, title={Correlative Instrumental Neutron Activation Analysis, LM, And TEM to Track in Vivo Distribution of Colloidal Gold Spheres in BALB/C Mice}, volume={4}, ISSN={1431-9276}, url={http://dx.doi.org/10.1017/s1431927600025113}, DOI={10.1017/s1431927600025113}, number={S2}, journal={Microscopy and Microanalysis}, publisher={Oxford University Press (OUP)}, author={Hillyer, JF and Albrecht, RM}, year={1998}, month=jul, pages={998–999} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        10
      ]
    ],
    "date-time": "2024-09-10T00:07:15Z",
    "timestamp": 1725926835167
  },
  "reference-count": 5,
  "publisher": "Oxford University Press (OUP)",
  "issue": "S2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1998,
            7,
            1
          ]
        ],
        "date-time": "1998-07-01T00:00:00Z",
        "timestamp": 899251200000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1998,
        7,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Colloidal gold is routinely used conjugated to ligands or antibodies as a label for the detection of cell structures by transmission (TEM) and scanning (SEM) electron microscopy. Silver enhancement, a procedure that amplifies the size of gold spheres, is used for light microscopic (LM) observation of colloidal gold. These techniques are valuable in localization studies but it is difficult to quantitate the level of labeling in larger volumes of tissue solely by microscopic means.</jats:p>\n               <jats:p>Instrumental Neutron Activation Analysis (INAA) can be used to provide a quantitative index of gold accumulation at the organ and tissue levels. Given that gold is not naturally found in the body in any substantial amount and that colloidal gold and ligand conjugates can be prepared to yield uniform bead sizes, the amount of label can be determined at the organ and tissue levels in whole animal systems.</jats:p>",
  "DOI": "10.1017/s1431927600025113",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        7,
        6
      ]
    ],
    "date-time": "2020-07-06T05:33:02Z",
    "timestamp": 1594013582000
  },
  "page": "998-999",
  "source": "Crossref",
  "is-referenced-by-count": 2,
  "title": "Correlative Instrumental Neutron Activation Analysis, LM, And TEM to Track in Vivo Distribution of Colloidal Gold Spheres in BALB/C Mice",
  "prefix": "10.1093",
  "volume": "4",
  "author": [
    {
      "given": "JF",
      "family": "Hillyer",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Animal Health and Biomedical Sciences, University of Wisconsin - Madison , Madison, WI 53706"
        }
      ]
    },
    {
      "given": "RM",
      "family": "Albrecht",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Animal Health and Biomedical Sciences, University of Wisconsin - Madison , Madison, WI 53706"
        }
      ]
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        1998,
        7,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "S1431927600025113_ref005",
      "unstructured": "This research was funded in part by the advanced opportunity fellowship program, University of Wisconsin - Madison. The aid of Paul Sims, Richard Cashwell, and Randall Massey is greatly appreciated."
    },
    {
      "key": "S1431927600025113_ref003",
      "first-page": "773",
      "volume": "9",
      "author": "Darien",
      "year": "1995",
      "journal-title": "Scanning Microsc."
    },
    {
      "key": "S1431927600025113_ref004",
      "unstructured": "Keisch, B. , The atomic fingerprint: neutron activation analysis, United States Atomic Energy Commission, Technical Information Center (1972)."
    },
    {
      "key": "S1431927600025113_ref001",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02473198"
    },
    {
      "key": "S1431927600025113_ref002",
      "first-page": "151",
      "volume-title": "Immunocytochemistry: a practical approach",
      "author": "Albrecht",
      "year": "1993"
    }
  ],
  "container-title": "Microscopy and Microanalysis",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://academic.oup.com/mam/article-pdf/4/S2/998/59057529/mam0998.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://academic.oup.com/mam/article-pdf/4/S2/998/59057529/mam0998.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        9,
        9
      ]
    ],
    "date-time": "2024-09-09T17:29:41Z",
    "timestamp": 1725902981000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/mam/article/4/S2/998/6931233"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1998,
        7,
        1
      ]
    ]
  },
  "references-count": 5,
  "journal-issue": {
    "issue": "S2",
    "published-online": {
      "date-parts": [
        [
          1998,
          7,
          1
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          1998,
          7,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1017/S1431927600025113",
  "relation": {},
  "ISSN": [
    "1435-8115",
    "1431-9276"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        1998,
        7,
        1
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        1998,
        7,
        1
      ]
    ]
  }
}