DOI: 10.1017/s0424820100172863. High-resolution SEM of biological macromolecular complexes

High-resolution SEM of biological macromolecular complexes

10.1017/s0424820100172863

Crossref journal-article

Cambridge University Press (CUP)

Proceedings, annual meeting, Electron Microscopy Society of America (56)

Abstract

For HRSEM imaging of insulating specimens, thin “homogeneous” metal films with a thickness smaller than the SE-escape depth (1-2 nm for metals) and with a neglectable or very low BSE-coefficient (low Z e.g. Cr or Ge), continuous enveloping the surface fine structures, are supposed to reveal a high resolution topographic SE-1 signal (Joy 1984). Hermann and Müller (1991) have reported a resolution of about 3-4 nm on freeze-dried thin biological specimens, coated with Cr at low temperature (−80°C). High resolution SE-images were also shown on ft or W sputter coated, frozen-hydrated yeast cells (Walther et al., 1992 and Hermann and Müller, 1993) and on coated, freeze-dried macromolecular structures (Walther et al. 1992; Ya Chen et al. 1993).To study the obtainable resolution and the extractable surface information in SEM from a variety of coating techniques and metals, thin specimens (<20nm) were used as test specimens in order to reduce the signal contribution of the background (SEII).

Bibliography

Wepf, R., Aebi, U., Bremer, A., Haider, M., Tittmann, P., Zach, J., & Gross, H. (1994). High-resolution SEM of biological macromolecular complexes. Proceedings, Annual Meeting, Electron Microscopy Society of America, 52, 1026â1027.

Authors 7

R. Wepf (first)
U. Aebi (additional)
A. Bremer (additional)
M. Haider (additional)
P. Tittmann (additional)
J. Zach (additional)
H. Gross (additional)

References 7 Referenced 2

{'key': 'S0424820100172863_ref07', 'first-page': '49', 'author': 'Zach', 'year': '1992', 'journal-title': 'EM Vol. 1 EUREM 92 Granada'} / EM Vol. 1 EUREM 92 Granada by Zach (1992)
{'key': 'S0424820100172863_ref06', 'first-page': '751', 'author': 'Wepf', 'year': '1992', 'journal-title': 'EM Vol, 3 EUREM 92 Granada'} / EM Vol, 3 EUREM 92 Granada by Wepf (1992)
10.1111/j.1365-2818.1992.tb03259.x
{'key': 'S0424820100172863_ref04', 'first-page': '343', 'volume': '7', 'author': 'Hermann', 'year': '1993', 'journal-title': 'Scanning Microscopy'} / Scanning Microscopy by Hermann (1993)
10.1002/jemt.1060180414
{'key': 'S0424820100172863_ref01', 'first-page': '120', 'volume': '51', 'author': 'Chen', 'year': '1993', 'journal-title': 'Proc. Aim. EMSA Meet'} / Proc. Aim. EMSA Meet by Chen (1993)
10.1111/j.1365-2818.1984.tb00532.x

Dates

Type	When
Created	5 years, 2 months ago (June 18, 2020, 5:52 a.m.)
Deposited	5 years, 2 months ago (June 18, 2020, 5:53 a.m.)
Indexed	11 months, 1 week ago (Sept. 8, 2024, 7:42 a.m.)
Issued	31 years, 7 months ago (Jan. 1, 1994)
Published	31 years, 7 months ago (Jan. 1, 1994)
Published Online	5 years, 2 months ago (June 18, 2020)
Published Print	31 years, 7 months ago (Jan. 1, 1994)

Funders 0

None

BibTeX

@article{Wepf_1994, title={High-resolution SEM of biological macromolecular complexes}, volume={52}, ISSN={2690-1315}, url={http://dx.doi.org/10.1017/s0424820100172863}, DOI={10.1017/s0424820100172863}, journal={Proceedings, annual meeting, Electron Microscopy Society of America}, publisher={Cambridge University Press (CUP)}, author={Wepf, R. and Aebi, U. and Bremer, A. and Haider, M. and Tittmann, P. and Zach, J. and Gross, H.}, year={1994}, pages={1026–1027} }

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