DOI: 10.1017/s0424820100142803. A controlled environment system for vitrification of liquid tem samples

A controlled environment system for vitrification of liquid tem samples

10.1017/s0424820100142803

Crossref journal-article

Cambridge University Press (CUP)

Proceedings, annual meeting, Electron Microscopy Society of America (56)

Abstract

Vitrification of aqueous samples is becoming an important technique for visualization of biological and colloidal dispersions and aggregates by electron microscopy. Vitrification prevents phase separation, rearrangement and problems of electron optics interaction with a crystalline matrix and artifact formation due to crystal growth. Vitrification also reduces radiation damage in some samples. Water can be vitrified with ultrafast cooling (> 100,000 K/s) by rapid plunging into melting ethane. Electron diffraction of bulk water films, thinned by blotting and cryo-fixed by quenching, has provided direct evidence for vitrification.

Bibliography

Bellare, O. (1986). A controlled environment system for vitrification of liquid tem samples. Proceedings, Annual Meeting, Electron Microscopy Society of America, 44, 236â237.

Authors 1

Oayesh Bellare (first)

References 3 Referenced 4

10.1038/308032a0
3. Talmon, Y. , Colloids and Surfaces (in press).
2. Talmon, Y. , Adrian, M. and Dubochet, J. , J. Microscopy (in press).

Dates

Type	When
Created	5 years, 2 months ago (June 18, 2020, 7:29 a.m.)
Deposited	5 years, 2 months ago (June 18, 2020, 7:47 a.m.)
Indexed	1 year, 10 months ago (Oct. 22, 2023, 8:19 a.m.)
Issued	39 years ago (Aug. 1, 1986)
Published	39 years ago (Aug. 1, 1986)
Published Online	5 years, 2 months ago (June 18, 2020)
Published Print	39 years ago (Aug. 1, 1986)

Funders 0

None

BibTeX

@article{Bellare_1986, title={A controlled environment system for vitrification of liquid tem samples}, volume={44}, ISSN={2690-1315}, url={http://dx.doi.org/10.1017/s0424820100142803}, DOI={10.1017/s0424820100142803}, journal={Proceedings, annual meeting, Electron Microscopy Society of America}, publisher={Cambridge University Press (CUP)}, author={Bellare, Oayesh}, year={1986}, month=aug, pages={236–237} }

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