DOI: 10.1017/s0424820100128961. Three-dimensional reconstruction of the 40S ribosomal subunit from rabbit reticulocytes

Three-dimensional reconstruction of the 40S ribosomal subunit from rabbit reticulocytes

10.1017/s0424820100128961

Crossref journal-article

Cambridge University Press (CUP)

Proceedings, annual meeting, Electron Microscopy Society of America (56)

Abstract

We have reconstructed the 40S ribosomal subunit at a resolution of 4 nm using the single-exposure pseudo-conical reconstruction method of Radermacher et al.Small (40S) ribosomal subunits were Isolated from rabbit reticulocytes, applied to grids and negatively stained (0.5% uranyl acetate) in a manner that “sandwiches” the specimen between two layers of carbon. Regions of the grid exhibiting uniform and thick staining were identified and photographed twice (magnification 49,000X). The first micrograph was always taken with the specimen tilted by 50° and the second was of the Identical area untilted (Fig. 1). For each of the micrographs the specimen was subjected to an electron dose of 2000-3000 el/nm2.Three hundred thirty particles appearing in the L view (defined in [4]) were selected from both tilted- and untilted-specimen micrographs. The untilted particles were aligned and their rotational alignment produced the azimuthal angles of the tilted particles in the conical tilt series.

Bibliography

Zhang, N.-Y., Wagenknecht, T., Radermacher, M., Obrig, T., & Frank, J. (1987). Three-dimensional reconstruction of the 40S ribosomal subunit from rabbit reticulocytes. Proceedings, Annual Meeting, Electron Microscopy Society of America, 45, 936â937.

Authors 5

Neng-Yu Zhang (first)
Terence Wagenknecht (additional)
Michael Radermacher (additional)
Tom Obrig (additional)
Joachim Frank (additional)

References 7 Referenced 2

Supported by NIH grant IROI GM29169 and NSF grant PCM8312045.
10.1126/science.7313694
10.1515/9783111439846-018
Radermacher, M. Wagenknecht, T. , Verschoor, A. and Frank, J. , J. Microsc., in press.
10.1073/pnas.75.6.2829
10.1016/0304-3991(82)90239-X
10.1073/pnas.68.2.390

Dates

Type	When
Created	5 years, 2 months ago (June 18, 2020, 7:28 a.m.)
Deposited	5 years, 2 months ago (June 18, 2020, 7:33 a.m.)
Indexed	1 year, 10 months ago (Oct. 21, 2023, 2:55 p.m.)
Issued	38 years, 1 month ago (Aug. 1, 1987)
Published	38 years, 1 month ago (Aug. 1, 1987)
Published Online	5 years, 2 months ago (June 18, 2020)
Published Print	38 years, 1 month ago (Aug. 1, 1987)

Funders 0

None

BibTeX

@article{Zhang_1987, title={Three-dimensional reconstruction of the 40S ribosomal subunit from rabbit reticulocytes}, volume={45}, ISSN={2690-1315}, url={http://dx.doi.org/10.1017/s0424820100128961}, DOI={10.1017/s0424820100128961}, journal={Proceedings, annual meeting, Electron Microscopy Society of America}, publisher={Cambridge University Press (CUP)}, author={Zhang, Neng-Yu and Wagenknecht, Terence and Radermacher, Michael and Obrig, Tom and Frank, Joachim}, year={1987}, month=aug, pages={936–937} }

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