DOI: 10.1017/s0424820100127311. LVSEM: A new way of seeing biology

10.1017/s0424820100127311

Crossref journal-article

Cambridge University Press (CUP)

Proceedings, annual meeting, Electron Microscopy Society of America (56)

Abstract

When dried, soft tissue biological samples have a density of .3 to .02 g/cm3. In such material, the range of electrons having an energy of 20-30 keV can be tens or hundreds of microns, and electons emitted from any surface of the sample within this range can contribute to the detected secondary electron signal. However, only those originating from the immediate vicinity of the beam contribute useful topographic information. Those emerging farther away contribute not only a slowly varying noise signal, but the statistical variations in this signal constitute an important noise source. In many cases, it may be so great as to totally mask the signal variations due to topographic contrast. In biology, the limitations caused by this fact are usually ameliorated by coating the outer surface of the sample with 10-20 nm of a heavy metal such as gold (density = 19 g/cm3).

Bibliography

Pawley, J. B. (1987). LVSEM: A new way of seeing biology. Proceedings, Annual Meeting, Electron Microscopy Society of America, 45, 550â553.

Authors 1

J.B. Pawley (first)

References 8 Referenced 6

{'key': 'S0424820100127311_ref2', 'first-page': '28', 'article-title': 'Scanning Device Combined with Conventional Electron Microscope', 'volume': '29', 'author': 'Koike', 'year': '1971', 'journal-title': 'Proc. EMSA'} / Proc. EMSA / Scanning Device Combined with Conventional Electron Microscope by Koike (1971)
{'key': 'S0424820100127311_ref4', 'first-page': '2105', 'volume-title': 'Is There a Limit to the Resolving Power of the SEM?', 'author': 'Crewe'} / Is There a Limit to the Resolving Power of the SEM? by Crewe
Pawley, J .B. , Use of Pseudo-Stereo Techniques to Detect Magnetic Stray Field in the SEM, Scanning (in press).
10.1111/j.1365-2818.1984.tb02545.x
Nagatani, T. and Saito, S. , Instrumentation for Ultra High Resolution Scanning Electron Microscopy, in Imura, T. , Maruse, S. and Suzuki, T. , Eds., Electron Microscopy 1986, Proc. XI Inti. Cong. EM, Kyoto (1986) 2101.
Albrecht, R. , this volume.
10.1002/sca.4950040402
Thanks are due to Stan Erlandsen of the University of Minnesota and Hans Ris of the IMR for, respectively, figures 3/4 & 5. This work is supported by NIH grant #RR00570-16 to the Madison Integrated Microscopy Resource.

Dates

Type	When
Created	5 years, 2 months ago (June 18, 2020, 7:28 a.m.)
Deposited	5 years, 2 months ago (June 18, 2020, 7:52 a.m.)
Indexed	1 year, 10 months ago (Oct. 23, 2023, 2:58 a.m.)
Issued	38 years ago (Aug. 1, 1987)
Published	38 years ago (Aug. 1, 1987)
Published Online	5 years, 2 months ago (June 18, 2020)
Published Print	38 years ago (Aug. 1, 1987)

Funders 0

None

BibTeX

@article{Pawley_1987, title={LVSEM: A new way of seeing biology}, volume={45}, ISSN={2690-1315}, url={http://dx.doi.org/10.1017/s0424820100127311}, DOI={10.1017/s0424820100127311}, journal={Proceedings, annual meeting, Electron Microscopy Society of America}, publisher={Cambridge University Press (CUP)}, author={Pawley, J.B.}, year={1987}, month=aug, pages={550–553} }

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