DOI: 10.1017/s0424820100110477. Cryofixation of suspensions and tissues by propane-jet freezing and high-pressure freezing

Cryofixation of suspensions and tissues by propane-jet freezing and high-pressure freezing

10.1017/s0424820100110477

Crossref journal-article

Cambridge University Press (CUP)

Proceedings, annual meeting, Electron Microscopy Society of America (56)

Abstract

During the last few years, several techniques for the rapid freezing of thin aqueous layers of cells and tissues have been developed and successfully applied to various biological questions (spray-freezing, propane-jet freezing, metalmirror-freezing, plunge-freezing, high pressure freezing). For practical work, techniques where the biological material (mainly suspensions) is sandwiched between two metal platelets are most favourable, because the frozen sandwiches are easily freeze-fractured, freeze-substituted or freeze-dried. In addition, the thickness that is sufficiently well frozen (vitrified or in the microcrystalline state) is greater in a sandwiched sample than in a specimen cooled only from one side (1). Heat can be extracted from the specimen sandwich either by controlled rapid plunging into a suitable coolant (2) or by shooting the coolant simultaneously from opposite directions onto the specimen surface (propane-jet) (3).

Bibliography

MÃ¼ller, M., & Moor, H. (1984). Cryofixation of suspensions and tissues by propane-jet freezing and high-pressure freezing. Proceedings, Annual Meeting, Electron Microscopy Society of America, 42, 6â9.

Authors 2

Martin Müller (first)
Hans Moor (additional)

References 5 Referenced 14

10.1007/BF00237626
{'key': 'S0424820100110477_ref2', 'first-page': '361', 'volume': 'II', 'author': 'Costello', 'year': '1980', 'journal-title': 'Scanning Electron Microscopy'} / Scanning Electron Microscopy by Costello (1980)
{'key': 'S0424820100110477_ref3', 'first-page': '129', 'volume': '36', 'author': 'Müller', 'year': '1980', 'journal-title': 'Mikroskopie (Wien)'} / Mikroskopie (Wien) by Müller (1980)
{'key': 'S0424820100110477_ref1', 'first-page': '31', 'volume-title': 'Sociētē Françhise de Microscopie Electronique', 'author': 'Riehle', 'year': '1973'} / Sociētē Françhise de Microscopie Electronique by Riehle (1973)
{'key': 'S0424820100110477_ref4', 'volume-title': 'Proc. 4th Congr. on EM', 'author': 'Moor', 'year': '1968'} / Proc. 4th Congr. on EM by Moor (1968)

Dates

Type	When
Created	5 years, 2 months ago (June 18, 2020, 8:06 a.m.)
Deposited	5 years, 2 months ago (June 18, 2020, 8:08 a.m.)
Indexed	1 month, 1 week ago (July 11, 2025, 6:52 a.m.)
Issued	41 years ago (Aug. 1, 1984)
Published	41 years ago (Aug. 1, 1984)
Published Online	5 years, 2 months ago (June 18, 2020)
Published Print	41 years ago (Aug. 1, 1984)

Funders 0

None

BibTeX

@article{M_ller_1984, title={Cryofixation of suspensions and tissues by propane-jet freezing and high-pressure freezing}, volume={42}, ISSN={2690-1315}, url={http://dx.doi.org/10.1017/s0424820100110477}, DOI={10.1017/s0424820100110477}, journal={Proceedings, annual meeting, Electron Microscopy Society of America}, publisher={Cambridge University Press (CUP)}, author={Müller, Martin and Moor, Hans}, year={1984}, month=aug, pages={6–9} }

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