Hydrogen exchange and structural dynamics of proteins and nucleic acids
10.1017/s0033583500005217
Crossref journal-article
Cambridge University Press (CUP)
Quarterly Reviews of Biophysics (56)
Abstract

Though the structures presented in crystallographic models of macromolecules appear to possess rock-like solidity, real proteins and nucleic acids are not particularly rigid. Most structural work to date has centred upon the native state of macromolecules, the most probable macromolecular form. But the native state of a molecule is merely its most abundant form, certainly not its only form. Thermodynamics requires that all other possible structural forms, however improbable, must also exist, albeit with representation corresponding to the factor exp( —Gi/RT) for each state of free energyGi(see Moelwyn-Hughes, 1961), and one appreciates that each molecule within a population of molecules will in time explore the vast ensemble ofpossiblestructural states.

Bibliography

Englander, S. W., & Kallenbach, N. R. (1983). Hydrogen exchange and structural dynamics of proteins and nucleic acids. Quarterly Reviews of Biophysics, 16(4), 521–655.

Authors 2
  1. S. Walter Englander (first)
  2. Neville R. Kallenbach (additional)
References 244 Referenced 1,051
  1. 10.1063/1.1731411
  2. 10.1021/ja00960a040
  3. 10.1016/0022-2836(78)90053-0
  4. 10.1016/0022-2836(74)90420-3
  5. 10.1016/S0065-3233(08)60190-4
  6. 10.1016/S0006-3495(80)84989-7
  7. 10.1016/0022-2836(79)90548-5
  8. {'key': 'S0033583500005217_ref233', 'first-page': '549', 'volume-title': 'Protein Folding', 'author': 'Wüthrich', 'year': '1980'} / Protein Folding by Wüthrich (1980)
  9. 10.1016/S0021-9258(18)62060-5 / J. biol. Chem. / The correlation of ribo-nuclease exchange kinetics with the temperature induced transition by Woodward (1971)
  10. 10.1073/pnas.66.4.1067
  11. 10.1146/annurev.bb.08.060179.000531
  12. 10.1016/S0021-9258(19)41918-2 / J. biol. Chem. / The solvent dependence of hydrogen exchange kinetics of folded proteins by Woodward (1975)
  13. 10.1016/S0021-9258(19)41917-0 / J. biol. Chem. / Solvent accessibility in folded proteins: studies of hydrogen exchange in trypsin by Woodward (1975)
  14. {'key': 'S0033583500005217_ref222', 'first-page': '223', 'article-title': 'Hydrogen isotope exchange in the study of protein conformation', 'volume': '38', 'author': 'Willumsen', 'year': '1971', 'journal-title': 'C. r. Trav. Lab. Carlsberg'} / C. r. Trav. Lab. Carlsberg / Hydrogen isotope exchange in the study of protein conformation by Willumsen (1971)
  15. 10.1021/j150626a006
  16. 10.1021/bi00277a025 / Biochemistry / The structure of apamin in solution: 2D NMR study by Wemmer (1983)
  17. 10.1021/ar00069a004
  18. 10.1016/0022-2836(71)90158-6
  19. 10.1016/0022-2836(79)90414-5
  20. 10.1038/275247a0
  21. 10.1016/0022-2836(82)90180-2
  22. 10.1007/BF00863706
  23. 10.1016/0006-291X(80)90308-3
  24. {'key': 'S0033583500005217_ref206', 'first-page': '261', 'article-title': 'Internal mobility in globular proteins', 'volume': '1', 'author': 'Wagner', 'year': '1982', 'journal-title': 'Comments Molec. Cell. Biophysics'} / Comments Molec. Cell. Biophysics / Internal mobility in globular proteins by Wagner (1982)
  25. 10.1111/j.1432-1033.1971.tb01634.x
  26. 10.1021/bi00539a001
  27. 10.1007/BF02910866
  28. 10.1016/0022-2836(75)90091-1
  29. 10.1246/bcsj.51.1988
  30. 10.1016/0022-2836(79)90539-4
  31. 10.1021/bi00711a028
  32. 10.1007/978-1-4612-6137-7
  33. 10.1021/bi00638a012
  34. 10.1016/0022-2836(76)90101-7
  35. {'key': 'S0033583500005217_ref184', 'first-page': '65', 'article-title': 'Real space refinement of neutron diffraction data from carbon monoxide sperm whale myoglobin', 'volume': '34', 'author': 'Schoenborn', 'year': '1978', 'journal-title': 'Acta. crystallogr.'} / Acta. crystallogr. / Real space refinement of neutron diffraction data from carbon monoxide sperm whale myoglobin by Schoenborn (1978)
  36. 10.1002/bip.1978.360170515
  37. 10.1021/ja01000a006
  38. 10.1073/pnas.71.10.4154
  39. {'key': 'S0033583500005217_ref177', 'volume-title': 'Biomolecular Stereodynamics', 'author': 'Sarma', 'year': '1981'} / Biomolecular Stereodynamics by Sarma (1981)
  40. 10.1016/S0021-9258(18)92009-0 / J. biol. Chem. / Studies of hydrogen exchange in proteins, i. The exchange kinetics of bovine carbonic anhydrase by Rosenberg (1968)
  41. 10.1063/1.1730390
  42. 10.1016/0022-2836(81)90318-1
  43. {'key': 'S0033583500005217_ref169', 'article-title': 'Exchange kinetics of individual amide protons in thermally unfolded BPTI', 'author': 'Roder', 'year': '1983', 'journal-title': 'Biochim. biophys. Acta'} / Biochim. biophys. Acta / Exchange kinetics of individual amide protons in thermally unfolded BPTI by Roder (1983)
  44. {'key': 'S0033583500005217_ref180', 'first-page': '230', 'article-title': 'The stability of hydrogen bonded peptide structures in aqueous solution', 'volume': '29', 'author': 'Schellman', 'year': '1955', 'journal-title': 'C. r. Lab. Carlsberg (Ser. Chim.)'} / C. r. Lab. Carlsberg (Ser. Chim.) / The stability of hydrogen bonded peptide structures in aqueous solution by Schellman (1955)
  45. Roder H. , Wagner G. & Wüthrich K. (1983 a). Kinetics and cooper-ativity of the solvent exchange of individual amide protons in BPTI. Biochim. biophys. Acta (in the Press).
  46. 10.1021/ja01028a005
  47. 10.1111/j.1432-1033.1979.tb04273.x
  48. 10.1016/S0065-3233(08)60520-3
  49. 10.1007/BF02906521
  50. 10.1146/annurev.bi.50.070181.004541
  51. 10.1016/S0065-3233(08)60460-X
  52. 10.1016/0968-0004(83)90063-4
  53. 10.1073/pnas.53.2.363
  54. {'key': 'S0033583500005217_ref157', 'first-page': '405', 'volume-title': 'Biomolecular Stereodynamics', 'author': 'Preisler', 'year': '1981'} / Biomolecular Stereodynamics by Preisler (1981)
  55. 10.1021/bi00846a052
  56. 10.1002/bip.360220135
  57. {'key': 'S0033583500005217_ref153', 'first-page': '1', 'article-title': 'Crystallographic studies of movement within proteins', 'volume': '46', 'author': 'Phillips', 'year': '1981', 'journal-title': 'Biochem. Soc. Symp.'} / Biochem. Soc. Symp. / Crystallographic studies of movement within proteins by Phillips (1981)
  58. 10.1126/science.6280281
  59. 10.1021/bi00268a038
  60. 10.3109/10409237509102551
  61. 10.1038/296776a0
  62. 10.1016/0022-2836(72)90504-9
  63. Roder H. (1981). Mobilitat in Proteinen unter nativen und denaturierden Bedingungen: Untersuchung von Trypsin Inhibitoren mit spectro-skopischen Methoden. Diss. ETH No. 6932.
  64. 10.1021/bi00686a019
  65. 10.1111/j.1399-3011.1981.tb01966.x
  66. {'key': 'S0033583500005217_ref195', 'first-page': '122', 'article-title': 'Protein denaturation', 'volume': '23', 'author': 'Tanford', 'year': '1968', 'journal-title': 'Adv. Protein Chem.'} / Adv. Protein Chem. / Protein denaturation by Tanford (1968)
  67. 10.1016/S0021-9258(18)70031-8 / J. biol. Chem. / The preparation of subtilisin-modified ribonuclease and the separation of the peptide and protein components by Richards (1959)
  68. 10.1021/bi00276a003
  69. 10.1021/bi00276a002
  70. 10.1016/S0006-3495(80)84912-5
  71. 10.1111/j.1749-6632.1975.tb41518.x
  72. 10.1016/0022-2836(80)90044-3
  73. 10.1016/0022-2836(76)90191-1
  74. 10.1016/S0006-3495(80)84984-8
  75. 10.1093/nar/9.24.7073
  76. 10.1016/B978-0-12-571803-5.50010-9
  77. 10.1016/S0021-9258(18)63519-7 / J. biol. Chem. / Studies of hydrogen exchange in proteins. II. The reversible thermal unfolding of chymotrypsinogen A as studied by exchange kinetics by Rosenberg (1969)
  78. 10.1002/bip.1981.360200116
  79. 10.1016/S0021-9258(19)45590-7 / J. biol. Chem. / Conformational changes in human serum albumin as revealed by hydrogen deuterium exchange studies by Hvidt (1972)
  80. 10.1246/bcsj.47.293
  81. 10.1021/bi00846a053
  82. 10.1107/S0567740875002415
  83. Connolly M. L. (1981). Molecular surfaces and interior cavities of proteins. Ph.D. Dissertation UCSF.
  84. 10.1016/S0021-9258(19)62340-9 / J. biol. Chem. / Pressure effects on folded proteins in solution by Carter (1978)
  85. 10.1073/pnas.73.8.2740
  86. 10.1111/j.1432-1033.1978.tb12425.x
  87. 10.1016/S0022-2836(83)80105-3
  88. 10.1016/S0006-3495(80)84990-3
  89. {'key': 'S0033583500005217_ref007', 'first-page': '217', 'volume-title': 'Protein Folding', 'author': 'Baldwin', 'year': '1980'} / Protein Folding by Baldwin (1980)
  90. 10.1016/S0021-9258(19)43744-7 / J. biol. Chem. / Structural and free energy changes in hemoglobin by use of a difference method by Englander (1973)
  91. 10.1146/annurev.bi.44.070175.002321
  92. 10.1038/280563a0
  93. 10.1016/0006-3002(54)90241-3
  94. 10.1021/bi00666a009
  95. 10.1016/0022-2836(79)90265-1
  96. Wüthrich K. , Wagner G. & Richarz R. (1978). A dynamic model for globular protein conformations based on high resolution NMR data. In Protein: Structure, Function and Industrial Applications, Proceedings of the I2th FEES Meeting, Dresden 1978 (ed. E. Hofmann, W. Pfeil and H. Aurich).
  97. 10.1016/0165-022X(83)90060-X
  98. 10.1016/0022-2836(82)90180-2
  99. 10.1146/annurev.bi.51.070182.002331
  100. 10.1016/S0021-9258(18)34285-6 / J. biol. Chem. / Reexamination of rhodopsin structure by hydrogen exchange by Englander (1982)
  101. {'key': 'S0033583500005217_ref161', 'first-page': '148', 'volume-title': 'Thermodynamics and Thermochemistry of Biologically Important Systems', 'author': 'Rialdi', 'year': '1975'} / Thermodynamics and Thermochemistry of Biologically Important Systems by Rialdi (1975)
  102. 10.1021/bi00516a015
  103. 10.1073/pnas.72.9.3290
  104. 10.1016/S0021-9258(19)45441-0 / J. biol. Chem. / Hydrogen exchange detection of discrete ligand-induced changes in hemoglobin by Englander (1972)
  105. 10.1021/bi00673a023
  106. 10.1002/hlca.19630460436
  107. {'key': 'S0033583500005217_ref035', 'first-page': '253', 'article-title': 'Solvent effects and approaches for the fine structure analysis of peptidyl amide 1H NMR spectra', 'volume': '39', 'author': 'DeMarco', 'year': '1980', 'journal-title': 'J. magn. Reson.'} / J. magn. Reson. / Solvent effects and approaches for the fine structure analysis of peptidyl amide 1H NMR spectra by DeMarco (1980)
  108. 10.1007/BF00863707
  109. 10.1016/0005-2795(79)90020-5
  110. 10.1021/ja00402a053
  111. 10.1016/0022-2836(74)90560-9
  112. 10.1073/pnas.76.12.6341
  113. 10.1021/ja01577a001
  114. 10.1021/ja00722a002
  115. 10.1021/bi00745a028
  116. {'key': 'S0033583500005217_ref220', 'first-page': '1944', 'article-title': 'Binding kinetics of mercury (II) to polynucleotides', 'volume': '14', 'author': 'Williams', 'year': '1975', 'journal-title': 'J. molec. Biol.'} / J. molec. Biol. / Binding kinetics of mercury (II) to polynucleotides by Williams (1975)
  117. 10.1016/0022-2836(71)90324-X
  118. 10.1101/SQB.1972.036.01.019
  119. 10.1021/ja00521a006
  120. 10.1016/S0021-9258(19)44353-6 / J. biol. Chem. / The solution conformation of the ferrichromes by Llinas (1973)
  121. 10.1016/S0006-3495(80)84913-7
  122. 10.1021/bi00517a008
  123. 10.1021/bi00593a013
  124. 10.1016/S0021-9258(19)44354-8 / J. biol. Chem. / The solution conformation of the ferrichromes by Llinas (1973)
  125. 10.1021/bi00573a001
  126. Schinkle J. (1983). Protein hydration dependence of the amide hydrogen exchange of lysozyme. Ph.D. Dissertation, University of Arizona, Tucson.
  127. {'key': 'S0033583500005217_ref117', 'volume-title': 'Symposium on Protein Structure', 'author': 'Linderstrøm-Lang', 'year': '1958'} / Symposium on Protein Structure by Linderstrøm-Lang (1958)
  128. 10.1021/bi00564a001
  129. {'key': 'S0033583500005217_ref138', 'first-page': '27', 'volume-title': 'Physical Chemistry', 'author': 'Moelwyn-Hughes', 'year': '1961'} / Physical Chemistry by Moelwyn-Hughes (1961)
  130. 10.1073/pnas.77.12.7222
  131. 10.1016/0022-2836(69)90105-3
  132. 10.1021/bi00745a020
  133. 10.1016/S0022-2836(77)80112-5
  134. 10.1016/S0022-2836(83)80235-6
  135. 10.1002/anie.196400011
  136. 10.1016/0022-2836(73)90096-X
  137. 10.1016/S0022-2836(83)80185-5
  138. 10.1073/pnas.80.18.5569
  139. 10.1002/bip.1964.360020507
  140. 10.1063/1.437838
  141. 10.1016/S0065-3233(08)60129-1
  142. 10.1021/ja01510a014
  143. 10.1103/PhysRevLett.44.1160
  144. 10.1021/j100804a509
  145. 10.1021/bi00658a014
  146. 10.1002/bip.1973.360120809
  147. 10.1021/bi00530a001
  148. 10.1016/0079-6107(83)90015-9
  149. 10.1016/S0021-9258(19)46935-4 / J. biol. Chem. / Is protein turnover thermodyn-amically controlled? by McLendon (1978)
  150. 10.1002/bip.1977.360160311
  151. 10.1017/S0033583500004911
  152. {'key': 'S0033583500005217_ref122', 'volume-title': 'Dynamic Properties of Polyion Systems', 'author': 'Lumry', 'year': '1978'} / Dynamic Properties of Polyion Systems by Lumry (1978)
  153. 10.1002/bip.1968.360060904
  154. 10.1002/bip.1980.360190509
  155. {'key': 'S0033583500005217_ref009', 'volume-title': 'Determination of pH: Theory and Practice', 'author': 'Bates', 'year': '1964'} / Determination of pH: Theory and Practice by Bates (1964)
  156. Levitt M. (1983). Molecular dynamics of native protein. J. molec. Biol. (in the Press).
  157. 10.1016/S0021-9258(18)63395-2 / J. biol. Chem. / The 3-dimensional structuring of ribo-nuclease S. Interpretation of an electron density map at nominal resolution of 2 Å by Wyckoff (1970)
  158. 10.1073/pnas.68.6.1203
  159. 10.1073/pnas.68.6.1199
  160. 10.1111/j.1749-6632.1981.tb50567.x
  161. 10.1073/pnas.74.10.4130
  162. 10.1016/S0021-9258(18)62061-7 / J. biol. Chem. / Urea effects on hydrogen exchange kinetics leading to a general model for hydrogen exchange from folded proteins by Woodward (1971)
  163. 10.1038/228726a0
  164. 10.1073/pnas.53.2.370
  165. 10.1016/0022-2836(72)90527-X
  166. 10.1016/0003-9861(57)90478-2
  167. 10.1016/S0022-2836(83)80186-7
  168. {'key': 'S0033583500005217_ref079', 'first-page': '299', 'article-title': 'Discussion of the pH dependence of the H-D exchange of proteins', 'volume': '34', 'author': 'Hvidt', 'year': '1964', 'journal-title': 'C. r. Trav. Lab. Carlsberg'} / C. r. Trav. Lab. Carlsberg / Discussion of the pH dependence of the H-D exchange of proteins by Hvidt (1964)
  169. 10.1016/0003-2697(81)90474-7
  170. 10.1016/S0006-3495(80)84991-5
  171. 10.1146/annurev.bi.41.070172.004351
  172. 10.1002/bip.1969.360070309
  173. 10.1002/bbpc.19820860511
  174. 10.1038/280558a0
  175. {'key': 'S0033583500005217_ref062', 'volume-title': 'The Theory of Rate Processes', 'author': 'Glasstone', 'year': '1941'} / The Theory of Rate Processes by Glasstone (1941)
  176. 10.1016/S0022-2836(77)80066-1
  177. 10.1016/0022-2836(72)90227-6
  178. {'key': 'S0033583500005217_ref108', 'first-page': '720', 'article-title': 'Origin of the absorption band at 1550 cm−1 in proteins', 'volume': '172', 'author': 'Lenormant', 'year': '1953', 'journal-title': 'Nature'} / Nature / Origin of the absorption band at 1550 cm−1 in proteins by Lenormant (1953)
  179. 10.1021/bi00534a042
  180. 10.1021/bi00268a031
  181. 10.1016/S0065-3233(08)60459-3
  182. 10.1021/bi00864a032
  183. 10.1021/bi00537a020
  184. 10.1021/j100872a049
  185. 10.1038/261552a0
  186. 10.1038/224175a0
  187. 10.1021/bi00519a027
  188. 10.1016/0022-2836(81)90343-0
  189. 10.1021/bi00609a024
  190. 10.1002/bip.360220136
  191. 10.1007/978-94-010-2579-9_6
  192. {'key': 'S0033583500005217_ref085', 'first-page': '1', 'article-title': 'Isotope exchange and Conformational fluctuation in polypeptides', 'volume': '6', 'author': 'Ikegami', 'year': '1974', 'journal-title': 'Adv. Biophys'} / Adv. Biophys / Isotope exchange and Conformational fluctuation in polypeptides by Ikegami (1974)
  193. 10.1021/bi00658a015
  194. 10.1016/S0021-9258(19)70363-9 / J. biol. Chem. / The slowest allosterically responsive hydrogens in hemoglobin: completion of the hydrogen exchange survey by Malin (1980)
  195. 10.1073/pnas.79.6.1849
  196. 10.1016/0022-2836(67)90215-X
  197. 10.1021/ja00752a007
  198. Kallenbach N. R. & Kim P. S. (1984). Effects of bases on fluctuations at the heme pocket of cyanometmyoglobin. Proc. natn. Acad. Sci. U.S.A. (in the Press).
  199. 10.1016/0022-2836(79)90549-7
  200. {'key': 'S0033583500005217_ref051', 'first-page': '421', 'volume-title': 'Structure and Dynamics of Nucleic Acids and Proteins', 'author': 'Englander', 'year': '1983'} / Structure and Dynamics of Nucleic Acids and Proteins by Englander (1983)
  201. 10.1016/S0079-6603(08)60120-8
  202. 10.1016/S0022-2836(65)80285-6
  203. 10.3109/10409238109105437
  204. 10.1016/S0065-3233(08)60608-7
  205. 10.1073/pnas.79.18.5537
  206. 10.1016/S0065-3233(08)60241-7
  207. {'key': 'S0033583500005217_ref097', 'first-page': '25', 'article-title': 'Non-covalent bonds in protein structure', 'volume': '13', 'author': 'Klotz', 'year': '1960', 'journal-title': 'Brookhaven Symp. Biol.'} / Brookhaven Symp. Biol. / Non-covalent bonds in protein structure by Klotz (1960)
  208. 10.1021/bi00865a047
  209. 10.1038/296713a0
  210. 10.1021/bi00745a021
  211. 10.1002/bip.360220703
  212. {'key': 'S0033583500005217_ref124', 'first-page': '55', 'article-title': 'The mobile defect hypothesis of protein function', 'volume': '246', 'author': 'Lumry', 'year': '1975', 'journal-title': "Col. Int. C.N.R.S. L'Eau Syst. Biol."} / Col. Int. C.N.R.S. L'Eau Syst. Biol. / The mobile defect hypothesis of protein function by Lumry (1975)
  213. 10.1021/bi00585a002
  214. 10.1002/bip.1981.360200810
  215. 10.1021/bi00876a037
  216. 10.1007/BF00421225
  217. 10.1038/294379a0
  218. 10.1146/annurev.bb.11.060182.001343
  219. 10.3109/10409237509102555
  220. 10.1016/S0021-9258(19)68727-2 / J. biol. Chem. / Carbon-13 spin lattice relaxation, linewidth and nuclear overhauser enhancement measurements of nucleosome length DNA by Levy (1981)
  221. 10.1002/bip.1979.360181008
  222. 10.1021/bi00262a026
  223. {'key': 'S0033583500005217_ref116', 'first-page': '1', 'article-title': 'Deuterium exchange between peptides and water', 'volume': '2', 'author': 'Linderstrøm-Lang', 'year': '1955', 'journal-title': 'Symposium on Peptide Chemistry. Chem. Soc. Spec. Publ.'} / Symposium on Peptide Chemistry. Chem. Soc. Spec. Publ. / Deuterium exchange between peptides and water by Linderstrøm-Lang (1955)
  224. {'key': 'S0033583500005217_ref118', 'volume-title': 'The Enzymes', 'volume': '1', 'author': 'Linderstrøm-Lang', 'year': '1959'} / The Enzymes by Linderstrøm-Lang (1959)
  225. 10.1016/0079-6107(79)90030-0
  226. 10.1038/267585a0
  227. 10.1016/0022-2836(70)90195-6
  228. 10.1021/bi00709a001
  229. 10.1021/bi00719a007
  230. 10.1016/0003-2697(79)90693-6
  231. 10.1021/bi00677a029
  232. 10.1016/0022-2836(79)90550-3
  233. 10.1016/0022-2836(79)90443-1
  234. {'key': 'S0033583500005217_ref134', 'volume-title': 'Neutrons in Biology: Neutron Scattering Analysis for Biological structures', 'author': 'Mason', 'year': '1983'} / Neutrons in Biology: Neutron Scattering Analysis for Biological structures by Mason (1983)
  235. {'key': 'S0033583500005217_ref200', 'first-page': '101', 'article-title': 'Overall and localized fluctuation in the structure of a protein molecule', 'volume': '12', 'author': 'Tsuboi', 'year': '1979', 'journal-title': 'Adv. Biophys.'} / Adv. Biophys. / Overall and localized fluctuation in the structure of a protein molecule by Tsuboi (1979)
  236. Matthew J. B. & Richards F. M. (1983). The pH dependence of hydrogen exchange in proteins. J. biol. Chem. (in the Press).
  237. 10.1063/1.443182
  238. 10.1016/0022-2836(73)90300-8
  239. 10.1016/0022-2836(79)90400-5
  240. 10.1021/bi00752a003
  241. 10.1021/ja00798a041
  242. 10.1021/ja00469a046
  243. 10.1073/pnas.79.5.1418
  244. 10.1016/0022-2836(72)90544-X
Dates
Type When
Created 16 years, 5 months ago (March 17, 2009, noon)
Deposited 3 years, 10 months ago (Oct. 3, 2021, 5:20 a.m.)
Indexed 3 weeks, 4 days ago (Aug. 5, 2025, 8:23 a.m.)
Issued 41 years, 9 months ago (Nov. 1, 1983)
Published 41 years, 9 months ago (Nov. 1, 1983)
Published Online 16 years, 5 months ago (March 17, 2009)
Published Print 41 years, 9 months ago (Nov. 1, 1983)
Funders 0

None

@article{Englander_1983, title={Hydrogen exchange and structural dynamics of proteins and nucleic acids}, volume={16}, ISSN={1469-8994}, url={http://dx.doi.org/10.1017/s0033583500005217}, DOI={10.1017/s0033583500005217}, number={4}, journal={Quarterly Reviews of Biophysics}, publisher={Cambridge University Press (CUP)}, author={Englander, S. Walter and Kallenbach, Neville R.}, year={1983}, month=nov, pages={521–655} }