Functional reassembly of membrane proteins in planar lipid bilayers
10.1017/s0033583500002079
Crossref journal-article
Cambridge University Press (CUP)
Quarterly Reviews of Biophysics (56)
Abstract

Recent progress in membrane biology has brought us to a stage where it is possible to associate complex biological processes to identifiable membrane proteins. Technical advances in the biochemical characterization and purification of membrane proteins have contributed a wealth of structural information. The reconstitution approach has proved to be valuable in our efforts to understand the molecular mechanisms of membrane transport and energy transduction.

Bibliography

Montal, M., Darszon, A., & Schindler, H. (1981). Functional reassembly of membrane proteins in planar lipid bilayers. Quarterly Reviews of Biophysics, 14(1), 1–79.

Authors 3
  1. M. Montal (first)
  2. A. Darszon (additional)
  3. H. Schindler (additional)
References 226 Referenced 99
  1. 10.1073/pnas.77.2.952
  2. 10.1085/jgp.75.3.241
  3. {'key': 'S0033583500002079_ref221', 'first-page': '146', 'volume-title': 'Handbook of Sensory Physiology', 'author': 'Yoshizawa', 'year': '1972'} / Handbook of Sensory Physiology by Yoshizawa (1972)
  4. 10.1111/j.1749-6632.1978.tb41981.x
  5. 10.1016/0006-291X(79)90938-0
  6. 10.1085/jgp.73.5.629
  7. 10.1016/0304-4173(79)90014-4
  8. 10.1016/S0021-9258(19)86687-5 / J. biol. Chem. / A voltage-gated anion channel from the electric organ of Torpedo californica by White (1979)
  9. {'key': 'S0033583500002079_ref213', 'first-page': '3238', 'article-title': 'A light-activated GTPase in vertebrate photoreceptors: regulation of light activated cyclic GMP phosphodiesterase', 'volume': '74', 'author': 'Wheeler', 'year': '1979', 'journal-title': 'Proc. natn. Acad. Sci. U.S.A.'} / Proc. natn. Acad. Sci. U.S.A. / A light-activated GTPase in vertebrate photoreceptors: regulation of light activated cyclic GMP phosphodiesterase by Wheeler (1979)
  10. 10.1016/0006-291X(74)90254-X
  11. 10.1016/0009-3084(76)90023-2
  12. 10.1038/266655a0
  13. 10.1111/j.1751-1097.1979.tb07093.x
  14. 10.1515/znb-1971-0527 / Z. Naturf. B / Flash photolysis of rhodopsin. I. Measurements on bovine rod outer segments by Von (1971)
  15. 10.1016/0005-2736(79)90392-4
  16. 10.1111/j.1751-1097.1980.tb03800.x
  17. 10.1016/0304-4173(79)90006-5
  18. 10.1021/ar50154a001
  19. 10.1073/pnas.73.2.396
  20. 10.1085/jgp.70.6.771
  21. 10.1085/jgp.74.5.595
  22. 10.1007/BF00743196
  23. 10.1007/BF01868147
  24. 10.1016/0014-5793(79)81199-0
  25. {'key': 'S0033583500002079_ref083', 'first-page': '41', 'volume-title': 'Vertebrate Photoreception', 'author': 'Hubbell', 'year': '1977'} / Vertebrate Photoreception by Hubbell (1977)
  26. 10.1085/jgp.36.2.269
  27. 10.1016/S0006-3495(78)85512-X
  28. 10.1146/annurev.bb.06.060177.000511
  29. 10.1016/0014-5793(78)80157-4 / FEBS Lett. / Time resolution of the intermediate steps in the bacteriorhodopsin-linked electrogenesis by Drachev (1978)
  30. 10.1038/249321a0
  31. 10.1016/0005-2736(78)90375-9
  32. 10.1146/annurev.bi.47.070178.001533
  33. {'key': 'S0033583500002079_ref067', 'first-page': '97', 'volume-title': 'Vertebrate Photoreception', 'author': 'Hagins', 'year': '1977'} / Vertebrate Photoreception by Hagins (1977)
  34. 10.1016/0006-291X(72)90240-9
  35. 10.1038/286395a0
  36. 10.1016/0301-4622(80)80049-4
  37. {'key': 'S0033583500002079_ref060', 'first-page': '261', 'volume-title': 'Ions in hydrocarbons,', 'author': 'Gemant', 'year': '1962'} / Ions in hydrocarbons, by Gemant (1962)
  38. 10.1007/BF01874112
  39. 10.1021/bi00574a004
  40. 10.1016/0022-2836(79)90037-8
  41. 10.1016/S0021-9258(19)42066-8 / Escherichia coli. J. biol. Chem. / Characterization of the major envelope protein from by Rosenbusch (1974)
  42. 10.1016/0005-2728(78)90144-5
  43. 10.1083/jcb.85.3.823
  44. 10.1073/pnas.77.9.5557
  45. 10.1016/S0006-3495(79)85316-3
  46. 10.1038/279643a0
  47. 10.1038/268562a0
  48. 10.1021/bi00568a024
  49. 10.1016/S0021-9258(17)37967-X / J. biol. Chem. / Reconstitution of carbamylcholinedependent sodium ion flux and desensitization of the acetylcholine receptor from Torpedo californica by Epstein (1978)
  50. 10.1085/jgp.75.5.469
  51. 10.1007/BF01869673
  52. 10.1016/0006-291X(77)91574-1
  53. {'key': 'S0033583500002079_ref125', 'volume-title': 'Chemiosmotic Coupling and Energy Transduction', 'author': 'Mitchell', 'year': '1968'} / Chemiosmotic Coupling and Energy Transduction by Mitchell (1968)
  54. 10.1016/S0021-9258(17)33259-3 / J. biol. Chem. / Distribution of enzyme activities in subcellular fractions of bovine retina by Zimmerman (1976)
  55. 10.1101/SQB.1976.040.01.023
  56. 10.1111/j.1432-1033.1980.tb04819.x
  57. 10.1016/0014-5793(79)80498-6
  58. {'key': 'S0033583500002079_ref169', 'first-page': '196', 'article-title': 'A light activated GTPase in retinal rod outer sements', 'volume': '17', 'author': 'Robinson', 'year': '1974', 'journal-title': 'Biophys. J.'} / Biophys. J. / A light activated GTPase in retinal rod outer sements by Robinson (1974)
  59. 10.1016/S0021-9258(18)43718-0 / J. biol. Chem. / Assembly of the mitochondrial membrane system. Sequence of the oxi2 gene of yeast mitochondria DNA by Thalenfold (1980)
  60. {'key': 'S0033583500002079_ref194', 'first-page': '31–73', 'volume-title': 'Energy Transducing Mechanisms', 'author': 'Skulachev', 'year': '1975'} / Energy Transducing Mechanisms by Skulachev (1975)
  61. 10.1021/bi00746a033
  62. 10.1073/pnas.74.1.207
  63. {'key': 'S0033583500002079_ref004', 'first-page': '122', 'article-title': 'Formation and properties of cell-size single bilayer vesicles', 'volume': '21', 'author': 'Antanavage', 'year': '1978', 'journal-title': 'Biophys. J.'} / Biophys. J. / Formation and properties of cell-size single bilayer vesicles by Antanavage (1978)
  64. {'key': 'S0033583500002079_ref222', 'first-page': '1', 'volume-title': 'Methods in Membrane Biology', 'author': 'Zahler', 'year': '1977'} / Methods in Membrane Biology by Zahler (1977)
  65. 10.1016/B978-0-12-511050-1.50038-7
  66. 10.1073/pnas.76.2.690
  67. 10.1126/science.7384786
  68. 10.1073/pnas.77.5.3052
  69. 10.1016/S0021-9258(19)70677-2 / J. biol. Chem. / Energy-transducing H+-ATP-ase of Escherichia coli. Reconstitution of proton translocation activity of the intrinsic membrane sector by Negrin (1980)
  70. 10.1073/pnas.77.3.1283
  71. 10.1007/BF00423232
  72. 10.1016/S0006-291X(73)80083-X
  73. 10.1113/jphysiol.1973.sp010410
  74. 10.1101/SQB.1976.040.01.022
  75. 10.1007/BF01868148
  76. 10.1021/bi00553a036
  77. 10.1016/0005-2736(78)90269-9
  78. {'key': 'S0033583500002079_ref202', 'first-page': '107', 'article-title': 'A new method for the formation of bilayer membranes in aqueous solution', 'volume': '13', 'author': 'Takagi', 'year': '1965', 'journal-title': 'Annu. Rep. Biol. Works. Fac. Sci. Osaka Univ.'} / Annu. Rep. Biol. Works. Fac. Sci. Osaka Univ. / A new method for the formation of bilayer membranes in aqueous solution by Takagi (1965)
  79. 10.1016/0014-5793(80)81083-0
  80. 10.1146/annurev.bb.01.060172.001023
  81. 10.1085/jgp.75.5.493
  82. {'key': 'S0033583500002079_ref091', 'first-page': '191', 'volume-title': 'Cell Surface Reviews', 'author': 'Karlin', 'year': '1980'} / Cell Surface Reviews by Karlin (1980)
  83. {'key': 'S0033583500002079_ref013', 'first-page': '553', 'article-title': 'Light- and GTP-activated photoreceptor PDE regulation by a light-activated GTPase and identification of rhodopsin as the binding site', 'volume': '9', 'author': 'Bitensky', 'year': '1978', 'journal-title': 'Adv. Cyclic Nucleotide Res.'} / Adv. Cyclic Nucleotide Res. / Light- and GTP-activated photoreceptor PDE regulation by a light-activated GTPase and identification of rhodopsin as the binding site by Bitensky (1978)
  84. 10.1016/0014-5793(79)80913-8
  85. 10.1038/285140a0
  86. 10.1146/annurev.ne.02.030179.000313
  87. 10.1016/0005-2728(76)90114-6
  88. 10.1007/BF02426663
  89. 10.1038/278643a0
  90. 10.1085/jgp.75.3.251
  91. 10.1016/S0022-2836(77)80167-8
  92. 10.1042/bj1820149
  93. 10.1111/j.1432-1033.1978.tb12209.x
  94. 10.1016/0005-2736(89)90002-3
  95. 10.1016/0304-4157(79)90002-9
  96. 10.1016/S0021-9258(19)85942-2 / J. biol. Chem. / Mitochondrial outer membrane contains a protein-producing non-specific diffusion channels by Zalman (1980)
  97. {'key': 'S0033583500002079_ref028', 'first-page': '345', 'volume-title': 'Handbook of Sensory Physiology', 'author': 'Cone', 'year': '1971'} / Handbook of Sensory Physiology by Cone (1971)
  98. 10.1016/0006-291X(76)90913-X
  99. 10.1038/282189a0
  100. 10.1016/0014-5793(72)80743-9
  101. Feher G. & Okamura M. Y. (1978). Chemical composition and properties of reaction centres. In The Photosynthetic Bacteria (ed. Clayton R. K. and Sistrom W. R. ), pp. 349–386.
  102. 10.1021/bi00626a009
  103. {'key': 'S0033583500002079_ref023', 'first-page': '155', 'article-title': "Observation d'un photopotentiel transitoire de la rhodopsine en préparation vesiculaire de membranes de bâtonnets rétiniens", 'volume': '288', 'author': 'Chapron', 'year': '1979', 'journal-title': 'C. r. hebd. Séanc. Acad. Sci.'} / C. r. hebd. Séanc. Acad. Sci. / Observation d'un photopotentiel transitoire de la rhodopsine en préparation vesiculaire de membranes de bâtonnets rétiniens by Chapron (1979)
  104. 10.1083/jcb.81.2.446
  105. 10.1126/science.22932
  106. 10.1021/bi00588a003
  107. 10.1016/S0074-7742(08)60650-9
  108. 10.1038/282329a0
  109. 10.1113/jphysiol.1960.sp006489
  110. 10.1038/280807a0
  111. 10.1007/BF01870576
  112. 10.1042/bst0050491
  113. 10.1016/S0021-9258(17)34263-1 / J. biol. Chem. / Light-activated phosphodiesterase of the rod outer segment. Kinetics and parameters of activation and deactivation by Yee (1978)
  114. 10.1016/0005-2736(79)90171-8
  115. 10.1016/0014-5793(75)80492-3
  116. 10.1021/bi00590a027
  117. 10.1016/0014-5793(76)80829-0
  118. 10.1016/0014-5793(80)80405-4
  119. {'key': 'S0033583500002079_ref076', 'first-page': '1988', 'article-title': 'Electron transfer across membranes and energy coupling', 'volume': '32', 'author': 'Hinkle', 'year': '1973', 'journal-title': 'Fedn Proc. Fedn Am. Socs exp. Biol.'} / Fedn Proc. Fedn Am. Socs exp. Biol. / Electron transfer across membranes and energy coupling by Hinkle (1973)
  120. 10.1016/S0006-3495(78)85392-2
  121. 10.1152/physrev.1979.59.1.165
  122. {'key': 'S0033583500002079_ref024', 'first-page': '1599', 'article-title': 'The reconstitution of cytochrome oxidase proteolipid into bilayer membranes', 'volume': '35', 'author': 'Chien', 'year': '1976', 'journal-title': 'Fedn Proc. Fedn Am. Socs. exp. Biol.'} / Fedn Proc. Fedn Am. Socs. exp. Biol. / The reconstitution of cytochrome oxidase proteolipid into bilayer membranes by Chien (1976)
  123. 10.1113/jphysiol.1978.sp012431
  124. 10.1016/0014-5793(77)80770-9
  125. 10.1073/pnas.77.4.1796
  126. 10.1073/pnas.76.12.6351
  127. 10.1016/S0021-9258(19)41069-7 / J. biol. Chem. / Purification and properties of the light-activated cyclic nucleotide phosphodiesterase of rod outer segments by Miki (1975)
  128. {'key': 'S0033583500002079_ref005', 'first-page': '182', 'article-title': 'Rhodopsin mediated proton fluxes in lipid bilayers', 'volume': '17', 'author': 'Antanavage', 'year': '1977', 'journal-title': 'Biophys. J.'} / Biophys. J. / Rhodopsin mediated proton fluxes in lipid bilayers by Antanavage (1977)
  129. 10.1021/bi00549a001
  130. 10.1113/jphysiol.1972.sp009918
  131. 10.1016/0005-2728(76)90048-7
  132. 10.1016/S0021-9258(19)85623-5 / J. biol. Chem. / Dicyclohexylcarbodiimide binds specifically and covalently to cytochrome c oxidase while inhibiting its H+-translocating activity by Casey (1980)
  133. 10.1038/260799a0
  134. {'key': 'S0033583500002079_ref189', 'first-page': '159', 'volume-title': 'In Energy Conservation in Biological Membranes', 'author': 'Sebald', 'year': '1978'} / In Energy Conservation in Biological Membranes by Sebald (1978)
  135. 10.1038/286071a0
  136. 10.1007/BF01869042
  137. 10.1021/bi00893a017
  138. 10.1113/jphysiol.1972.sp009840
  139. 10.1007/978-3-642-66224-9_23
  140. {'key': 'S0033583500002079_ref010', 'first-page': '613', 'article-title': 'Responses of retinal rods to single photons', 'volume': '288', 'author': 'Baylor', 'year': '1979', 'journal-title': 'J. Pizysiol.'} / J. Pizysiol. / Responses of retinal rods to single photons by Baylor (1979)
  141. 10.1073/pnas.77.1.239
  142. 10.1016/0014-5793(79)81092-3
  143. 10.1021/bi00639a028
  144. 10.1016/S0021-9258(17)32940-X / J. Biol. Chem. / Reconstitution of biological molecular generators of electric current: Bacteriorhodopsin by Drachev (1976)
  145. 10.1007/BF01940934
  146. 10.1021/bi00708a031
  147. 10.1016/0005-2736(78)90049-4
  148. 10.1128/JB.107.3.718-735.1971 / J. Bact. / Molecular sieving by bacillus megaterium cell wall and protoplast by Scherrer (1971)
  149. 10.1016/0006-291X(80)91092-X
  150. 10.1146/annurev.ph.39.030177.000315
  151. 10.1016/0005-2728(78)90018-X
  152. 10.1016/0005-2728(72)90233-2
  153. 10.1073/pnas.77.5.2500
  154. 10.1038/280064a0
  155. 10.1016/0005-2736(80)90266-7
  156. 10.1021/bi00714a005
  157. 10.1152/physrev.1969.49.1.48
  158. Latorrs R. & Alvarez O. (1980). Voltage-dependent channels in planar lipid bilayers. Physiol. Rev. (in the Press).
  159. 10.1007/BF01869672
  160. 10.1016/0042-6989(79)90097-X
  161. 10.1113/jphysiol.1974.sp010733
  162. 10.1016/S0021-9258(19)70652-8 / J. biol. Chem. / Purification of acetyicholine receptors, reconstitution into lipid vesicles, and study of agonist-induced cation channel regulation by Lindstrom (1980)
  163. {'key': 'S0033583500002079_ref126', 'first-page': '591', 'volume-title': 'Perspectives in Membrane Biology', 'author': 'Montal', 'year': '1974'} / Perspectives in Membrane Biology by Montal (1974)
  164. 10.1021/bi00562a012
  165. {'key': 'S0033583500002079_ref113', 'first-page': '191', 'volume-title': 'Synaptic Transmission and Neuronal Interaction', 'author': 'Lindstrom', 'year': '1974'} / Synaptic Transmission and Neuronal Interaction by Lindstrom (1974)
  166. 10.1016/S0006-3495(75)85875-9
  167. 10.1016/S0021-9258(19)45651-2 / J. biol. Chem. / Ion transport and respiratory control in vesicles formed from cytochrome oxidase and phospholipids by Hinkle (1972)
  168. 10.1016/S0021-9258(17)40039-1 / J. biol. Chem. / Purified proton conductor in proton translocating adenosine triphosphatase of a thermophyllic bacterium by Okamoto (1977)
  169. 10.1111/j.1432-1033.1979.tb12859.x
  170. 10.1073/pnas.77.1.196
  171. 10.1146/annurev.bb.04.060175.002113
  172. 10.1038/267221a0
  173. 10.1038/287447a0
  174. 10.1152/physrev.1976.56.1.177
  175. 10.1007/BF01909736
  176. 10.1016/0304-4157(79)90003-0
  177. {'key': 'S0033583500002079_ref130', 'first-page': '177', 'volume-title': 'Vth Int. Biophys. Congr.', 'author': 'Montal', 'year': '1975'} / Vth Int. Biophys. Congr. by Montal (1975)
  178. 10.1016/S0021-9258(19)43080-9 / J. biol. Chem. / Reconstitution of purple membrane vesicles catalyzing light-driven proton uptake and adenosine triphosphate formation by Racker (1974)
  179. 10.1016/B978-0-12-225402-4.50045-0
  180. 10.1038/246219a0
  181. 10.1016/0005-2736(78)90374-7
  182. 10.1021/bi00604a001
  183. 10.1073/pnas.69.12.3561
  184. 10.1038/194979a0
  185. 10.1016/S0021-9258(17)33673-6 / J. biol. Chem. / Outer membrane of Salmonella. Isolation of protein complex that produces transmembrane channels by Nakae (1976)
  186. 10.1021/bi00582a028
  187. 10.1113/jphysiol.1978.sp012267
  188. 10.1073/pnas.77.5.3057
  189. 10.1016/S0021-9258(17)32992-7 / J. biol. Chem. / Purification of the carbodiimide-reactive protein component of the ATP energy-transducing system of Escherichia coli by Fillingame (1976)
  190. 10.1073/pnas.74.6.2375
  191. 10.1016/S0065-2911(08)60208-8
  192. 10.1146/annurev.bb.05.060176.001003
  193. Schindler H. (1980 b). Methods for reconstituting receptors in planar membranes. NRP Bulletin. (In the Press).
  194. 10.1016/0005-2736(79)90373-0
  195. {'key': 'S0033583500002079_ref015', 'first-page': '2151', 'article-title': 'Action spectrum and quantum efficiency for proton pumping', 'volume': '19', 'author': 'Bogomolni', 'year': '1980', 'journal-title': 'Halobacterium halobium. Biochemistry'} / Halobacterium halobium. Biochemistry / Action spectrum and quantum efficiency for proton pumping by Bogomolni (1980)
  196. 10.1101/SQB.1976.040.01.021
  197. 10.1073/pnas.74.12.5222
  198. 10.1016/0304-4173(77)90004-0
  199. 10.1016/0014-5793(79)80338-5
  200. 10.1073/pnas.77.2.928
  201. 10.1085/jgp.74.5.615
  202. 10.1016/0014-5793(80)80033-0
  203. 10.1016/S0006-3495(72)86145-9
  204. 10.1113/jphysiol.1957.sp005838
  205. 10.1021/bi00555a010
  206. 10.1126/science.111.2877.179
  207. 10.1016/0014-5793(79)81105-9
  208. 10.1042/bj1171011
  209. 10.1111/j.1749-6632.1980.tb15384.x
  210. {'key': 'S0033583500002079_ref167', 'first-page': '282', 'article-title': 'Acetyicholine receptors', 'volume': '7', 'author': 'Rang', 'year': '1975', 'journal-title': 'Q. Rev. Biophys.'} / Q. Rev. Biophys. / Acetyicholine receptors by Rang (1975)
  211. 10.1083/jcb.72.2.292
  212. 10.1085/jgp.76.4.397
  213. 10.1351/pac197749030341
  214. 10.1139/y62-145
  215. 10.1007/BF01869662
  216. 10.1073/pnas.75.8.3751
  217. Schindler H. & Rosenbusch J. P. (1981). Matrix protein in planar membranes: Clusters of channels in a native environment and their functional reassembly. Proc. natn. Acad. Sci. U.S.A. (In the Press.)
  218. {'key': 'S0033583500002079_ref097', 'first-page': '54', 'article-title': 'Reaction centres in hexane', 'volume': '25', 'author': 'Kendall-Tobias', 'year': '1979', 'journal-title': 'Biophys. J.'} / Biophys. J. / Reaction centres in hexane by Kendall-Tobias (1979)
  219. 10.1085/jgp.76.6.649
  220. 10.1016/0197-0186(80)90036-4
  221. 10.1073/pnas.77.2.785
  222. 10.1016/B978-0-12-152509-5.50011-7
  223. 10.1073/pnas.77.3.1408
  224. 10.1016/0006-291X(77)91391-2
  225. 10.1083/jcb.86.1.327
  226. 10.1111/j.1432-1033.1977.tb11874.x
Dates
Type When
Created 16 years, 5 months ago (March 17, 2009, 12:09 p.m.)
Deposited 3 years, 11 months ago (Oct. 3, 2021, 5:22 a.m.)
Indexed 4 months, 3 weeks ago (April 9, 2025, 12:47 a.m.)
Issued 44 years, 7 months ago (Feb. 1, 1981)
Published 44 years, 7 months ago (Feb. 1, 1981)
Published Online 16 years, 5 months ago (March 17, 2009)
Published Print 44 years, 7 months ago (Feb. 1, 1981)
Funders 0

None

@article{Montal_1981, title={Functional reassembly of membrane proteins in planar lipid bilayers}, volume={14}, ISSN={1469-8994}, url={http://dx.doi.org/10.1017/s0033583500002079}, DOI={10.1017/s0033583500002079}, number={1}, journal={Quarterly Reviews of Biophysics}, publisher={Cambridge University Press (CUP)}, author={Montal, M. and Darszon, A. and Schindler, H.}, year={1981}, month=feb, pages={1–79} }