Abstract
Commercially available electron microscopes routinely provide resolution of some 2–4 Å, as determined on the spacing of crystalline lattices of certain stable, small-molecular substances. On biological material either macromolecules or macromolecular assemblies— ‘biologically significant’ details below some 20 Å have hitherto not been observed.we consider as ‘biologically significant’ those structural details observed or contained in electronmicrographs which are consistent with, or confirmed by, other data obtained from biochemical or functional experiments or by other physical methods (optical, magnetic, electric).
References
116
Referenced
47
{'key': 'S0033583500001414_ref048', 'first-page': '16', 'volume-title': 'Image Processing and Computer Aided Design in Electron Optics', 'author': 'Hanszen', 'year': '1973'}
/ Image Processing and Computer Aided Design in Electron Optics by Hanszen (1973)10.1038/240404a0
{'key': 'S0033583500001414_ref040', 'first-page': '237', 'article-title': 'A practical algorithm for the determination of phase from image and diffraction plane pictures', 'volume': '35', 'author': 'Gerchberg', 'year': '1972', 'journal-title': 'Optik'}
/ Optik / A practical algorithm for the determination of phase from image and diffraction plane pictures by Gerchberg (1972)10.1126/science.179.4069.175
{'key': 'S0033583500001414_ref038', 'first-page': '29', 'article-title': 'Die ‘kritische Punkt’ Trocknung als Preparationsmethode für die Rasterelektronenmikroskopie', 'volume': '73', 'author': 'Fromme', 'year': '1972', 'journal-title': 'Microscopica Acta'}
/ Microscopica Acta / Die ‘kritische Punkt’ Trocknung als Preparationsmethode für die Rasterelektronenmikroskopie by Fromme (1972){'key': 'S0033583500001414_ref089', 'first-page': '416', 'article-title': 'Phase contrast in the scanning transmission electron microscope', 'volume': '39', 'author': 'Rose', 'year': '1974', 'journal-title': 'Optik'}
/ Optik / Phase contrast in the scanning transmission electron microscope by Rose (1974){'key': 'S0033583500001414_ref055', 'first-page': '123', 'article-title': 'Visualization of single atoms in molecules and crystals bydark-field electron microscopy', 'volume': '22', 'author': 'Hashimoto', 'year': '1973', 'journal-title': 'J. Electron Microsc'}
/ J. Electron Microsc / Visualization of single atoms in molecules and crystals bydark-field electron microscopy by Hashimoto (1973)10.1098/rstb.1971.0039
10.7551/mitpress/2946.001.0001
/ The extrapolation, interpolation and smoothing of stationary time series by Wiener (1949)- Downing K. H. (1972). Compensation of lens aberrations by single- sideband holography 30th Ann. Proc. EMSA, 562–3.
{'key': 'S0033583500001414_ref063', 'first-page': '538', 'article-title': 'Methods for reconstruction of complex image functions in electron microscopy', 'volume': '30', 'author': 'Hoppe', 'year': '1970', 'journal-title': 'Optik'}
/ Optik / Methods for reconstruction of complex image functions in electron microscopy by Hoppe (1970)10.1016/S0022-5320(70)80010-7
- Hanszen K.-J. (1973 b). Neuere theoretische Erkenntnisse und praktische Erfahrungen uber die holographische Rekonstruktion elektronenmikroskopischer Aufnahmen. Physikalisch-Technischen Bundesanstalt als PTB-Bericht A Ph. 4, Braunschweig.
{'key': 'S0033583500001414_ref059', 'first-page': '80', 'article-title': 'On the reconstruction of a weak phase-amplitude object: III', 'volume': '38', 'author': 'Hoenders', 'year': '1973', 'journal-title': 'Optik'}
/ Optik / On the reconstruction of a weak phase-amplitude object: III by Hoenders (1973)10.1126/science.175.4025.1000
- Glaeser R. M. , Kuo I. & Budinger T. F. (1971). Method for processing of periodicimages at reduced levels of electron irradiation. 29th Ann. Proc. EMSA, 466–7.
{'key': 'S0033583500001414_ref046', 'first-page': '21', 'article-title': 'In-line holographic reconstruction methods in electron microscopy', 'volume': 'I', 'author': 'Hanszen', 'year': '1970', 'journal-title': 'Proc. VIIth Int. Congr. Electron Microsc.'}
/ Proc. VIIth Int. Congr. Electron Microsc. / In-line holographic reconstruction methods in electron microscopy by Hanszen (1970)10.1098/rspb.1974.0034
10.1098/rstb.1971.0040
-
Wall J. (1972). Mass and mass loss measurements on DNA and fd phage. 30th Ann. Proc. EMSA, 186–7.
(
10.1017/S0424820100094188
) 10.1098/rspa.1970.0119
{'key': 'S0033583500001414_ref034', 'first-page': '196', 'volume-title': 'Image Processing and Computer-Aided Design in Electron Optics', 'author': 'Frank', 'year': '1973'}
/ Image Processing and Computer-Aided Design in Electron Optics by Frank (1973){'key': 'S0033583500001414_ref007', 'volume-title': 'Symp. Cryodécapage', 'author': 'Bachmann', 'year': '1973'}
/ Symp. Cryodécapage by Bachmann (1973)10.1016/S0006-3495(69)86441-6
10.1038/241445a0
10.1143/JJAP.8.1164
{'key': 'S0033583500001414_ref103', 'first-page': '55', 'article-title': 'Imaging of heavy atoms in dark field electron microscopyusing hollow cone illumination', 'volume': '36', 'author': 'Thon', 'year': '1972', 'journal-title': 'Optik'}
/ Optik / Imaging of heavy atoms in dark field electron microscopyusing hollow cone illumination by Thon (1972)- Henkelmann R. M. (1973). Ph.D. Thesis, University of Toronto.
{'key': 'S0033583500001414_ref081', 'first-page': 'L7', 'article-title': 'Phase determination from image intensity measurements in bright-field optics', 'volume': '7', 'author': 'Misell', 'year': '1974', 'journal-title': 'J. Phys.'}
/ J. Phys. / Phase determination from image intensity measurements in bright-field optics by Misell (1974){'key': 'S0033583500001414_ref102', 'first-page': '13', 'article-title': 'Zonal filtering in optical reconstruction of high-resolution phase-contrast images', 'volume': 'I', 'author': 'Thon', 'year': '1970', 'journal-title': 'VIIth hit. Congr. Electron Microsc., Grenoble'}
/ VIIth hit. Congr. Electron Microsc., Grenoble / Zonal filtering in optical reconstruction of high-resolution phase-contrast images by Thon (1970)10.1016/S0022-5320(71)80118-1
{'key': 'S0033583500001414_ref009', 'first-page': '244', 'article-title': 'Elektronenmikroskopische Untersuchungen bei atomarer Auflösung an Modelimembranen', 'volume': '7', 'author': 'Baumeister', 'year': '1973', 'journal-title': 'Cytobiologie'}
/ Cytobiologie / Elektronenmikroskopische Untersuchungen bei atomarer Auflösung an Modelimembranen by Baumeister (1973)10.1038/212029a0
10.1016/S0006-3495(71)86208-2
{'key': 'S0033583500001414_ref073', 'first-page': '1', 'article-title': 'The use of tilted specimen to obtain the contrast transfer characteristics of an electron microscope imaging system', 'volume': '40', 'author': 'Krakow', 'year': '1974', 'journal-title': 'Optik'}
/ Optik / The use of tilted specimen to obtain the contrast transfer characteristics of an electron microscope imaging system by Krakow (1974)- Hanszen K.-J. & Ade G. (1975). Die Bedeutung der Envelope von Übertragungsfunktionen bei partiell köharenter Beleuchtung. Optik (in preparation).
- Hanszen K.-J. & Ade G. (1974 b). Is it possible to describe the incoherent dark field imaging by transfer functions? Optik (in the press).
10.1016/S0022-5320(74)90015-X
- Ade G. (1972). Non-linear problems in bright and dark field imaging. Optik (in the Press).
- Ferwerda H. A. & Hoenders B. J. (1974 a). Theory of the reconstruction of a weak phase object in the case of non-isoplanatic imaging. Optica Acta (in the Press).
- Ferwerda H. A. (1974). Reconstruction of a weak phase-amplitude object from one or two exposures. (To be published.)
{'key': 'S0033583500001414_ref110', 'first-page': '412', 'article-title': 'Preparation of smooth graphite support films for high resolution electron microscopy', 'volume': '2', 'author': 'White', 'year': '1971', 'journal-title': 'Micron'}
/ Micron / Preparation of smooth graphite support films for high resolution electron microscopy by White (1971)10.1073/pnas.68.9.2149
10.1016/0022-2836(70)90181-6
10.1016/S0022-5320(72)80042-X
{'key': 'S0033583500001414_ref035', 'first-page': '582', 'article-title': 'A remark on phase determination in electron microscopy', 'volume': '38', 'author': 'Frank', 'year': '1973', 'journal-title': 'Optik'}
/ Optik / A remark on phase determination in electron microscopy by Frank (1973){'key': 'S0033583500001414_ref001', 'first-page': '196', 'volume-title': 'Principles and Techniques of Electron Microscopy', 'volume': '2', 'author': 'Abermann', 'year': '1972'}
/ Principles and Techniques of Electron Microscopy by Abermann (1972)10.1002/jss.400010606
10.1111/j.2164-0947.1951.tb01007.x
10.1111/j.2164-0947.1954.tb00377.x
10.1002/bbpc.19700741109
/ Ber. Bunsenges physik Chem. / The Fourier transform of an electron micrograph: effects of defocussing and aberrations, and implications for the use of underfocus contrast enhancement by Erickson (1970){'key': 'S0033583500001414_ref008', 'first-page': '1115', 'article-title': 'The elementary composition oforganic objects after electron irradiation', 'volume': '14', 'author': 'Bahr', 'year': '1965', 'journal-title': 'Lab. Invest.'}
/ Lab. Invest. / The elementary composition oforganic objects after electron irradiation by Bahr (1965)- Baumeister V. & Hahn M. H. (1973 c). Vermiculite single layers. J. Microscopy (in the Press).
10.1101/SQB.1972.036.01.063
- Bussler P. , Feltynowski A. & Hoppe W. (1972). Reconstruction of the complex image function from electron micrographs. Proc. Vth Europ. Congr. Electron Microsc., Manchester, 626–9.
10.1126/science.168.3937.1338
10.1101/SQB.1972.036.01.062
- Crowther R. A. , Amos L. A. & Klug A. (1972). Three-dimensional image reconstruction using functional expansions. Proc. Vth Europ. Congr. Electron Microsc., Manchester, pp. 593–7.
10.1038/217130a0
/ Nature / Reconstruction of 3-dimensional structures from electron micrographs by De (1968){'key': 'S0033583500001414_ref020', 'first-page': '21', 'article-title': 'Phase shift determination in single-sideband holography', 'volume': '38', 'author': 'Downing', 'year': '1973', 'journal-title': 'Optik'}
/ Optik / Phase shift determination in single-sideband holography by Downing (1973)-
Downing K. H. & Siegel B. M. (1973 b). Object wave determination by single sideband methods. 31st Ann. Proc. EMSA, 266–7.
(
10.1017/S0424820100071776
) - Hoppe W. & Köstler D. (1973). The inference of chromatic aberration and partial coherence for image reconstruction with tilted illumination. Joint session on Electron Microscopy, Li´ge.
{'key': 'S0033583500001414_ref025', 'first-page': '163', 'article-title': 'The Fourier transform of an electron micrograph - First-order and second-order theory of image formation', 'volume': '5', 'author': 'Erickson', 'year': '1973', 'journal-title': 'Adv. Opt. electron Microsc.'}
/ Adv. Opt. electron Microsc. / The Fourier transform of an electron micrograph - First-order and second-order theory of image formation by Erickson (1973)- Erickson H. P. (1974). Microtubule surface lattice and subunit structure. J. Cell Biol. (in the Press).
- Feltinowski A. & Hoppe W. (1973). Point resolution beyond the Scherzer limit in electron micrographs. Joint Session on Electron Microscopy, Liège.
10.1016/S0006-3495(72)86099-5
10.1098/rstb.1971.0048
{'key': 'S0033583500001414_ref036', 'first-page': '519', 'article-title': 'The envelope of electron microscopic transfer functions for partially coherent illumination', 'volume': '38', 'author': 'Frank', 'year': '1973', 'journal-title': 'Optik'}
/ Optik / The envelope of electron microscopic transfer functions for partially coherent illumination by Frank (1973)10.1002/bbpc.19700741105
/ Ber. Bunsenges / Einige Erfahrungen mit der rechnerischen Analyse und Synthese von elektronen mikroskopischen Bildern hoher Auflösung by Frank (1970)10.1016/0022-2836(72)90234-3
{'key': 'S0033583500001414_ref043', 'first-page': '326', 'article-title': 'An optical high-resolution spatial filtering and correlation unit for processingof electron micrographs', 'volume': '35', 'author': 'Hahn', 'year': '1972', 'journal-title': 'Optik'}
/ Optik / An optical high-resolution spatial filtering and correlation unit for processingof electron micrographs by Hahn (1972){'key': 'S0033583500001414_ref044', 'first-page': '224', 'article-title': 'Possibilities and limitations for electron microscopical imaging of single atoms in sub- and supramolecular systems', 'volume': '7', 'author': 'Hahn', 'year': '1973', 'journal-title': 'Cytobiologie'}
/ Cytobiologie / Possibilities and limitations for electron microscopical imaging of single atoms in sub- and supramolecular systems by Hahn (1973){'key': 'S0033583500001414_ref080', 'first-page': '168', 'volume-title': 'Image Processing and Computer-Aided Design in Electron Optics', 'author': 'Misell', 'year': '1973'}
/ Image Processing and Computer-Aided Design in Electron Optics by Misell (1973){'key': 'S0033583500001414_ref045', 'first-page': '125', 'article-title': 'Problems of image interpretation in electron microscopy with linear and non-linear transfer', 'volume': '27', 'author': 'Hanszen', 'year': '1969', 'journal-title': 'Z. angew. Phys.'}
/ Z. angew. Phys. / Problems of image interpretation in electron microscopy with linear and non-linear transfer by Hanszen (1969){'key': 'S0033583500001414_ref047', 'first-page': '1', 'article-title': 'The optical transfer theory of the electron microscope: Fundamental principles and applications', 'volume': '4', 'author': 'Hanszen', 'year': '1971', 'journal-title': 'Adv. Opt. and Electron Microsc'}
/ Adv. Opt. and Electron Microsc / The optical transfer theory of the electron microscope: Fundamental principles and applications by Hanszen (1971)- Hanszen K.-J. & Ade G. (1974 a). Problems and results of the optical transfer theory and of reconstruction methods in electron microscopy. Physikalisch-Technischen Bundesanstalt als PTB-Bericht A Ph. 5, Braunschweig.
- Hanszen K.-J. & Trepte L. (1971). The contrast transfer of the electron microscope with partial coherent illumination. Part A. The ring condensor. Part B: Disc-shaped source. Optik 33, 16681, 182–98.
10.1107/S0567739470001080
{'key': 'S0033583500001414_ref062', 'first-page': '612', 'volume-title': 'Proc. 5th Eur. Reg. Congr. Electron Microsc.', 'author': 'Hoppe', 'year': '1972'}
/ Proc. 5th Eur. Reg. Congr. Electron Microsc. by Hoppe (1972){'key': 'S0033583500001414_ref065', 'first-page': '92', 'volume-title': 'Image Processing and Computer-Aided Deszgn in Electron Optics', 'author': 'Hoppe', 'year': '1973'}
/ Image Processing and Computer-Aided Deszgn in Electron Optics by Hoppe (1973)- Koller TH. , Sogo J. M. & Bujard H. (1974). An electron microscopic method for studying nucleic acid-protein complexes. Visualization of E. coli RNA polymerase and T7 and T3 DNA. J. Polym. Sci. D (in the Press).
-
Krakow W. , Downing K. & Siegel B. (1973). A technique for the rapid determination of the contrast transfer characteristic of an electron microscope. 31st Ann. Proc. EMSA, 278–9.
(
10.1017/S0424820100071831
) 10.1515/zna-1971-0710
/ Z. Naturf. / Zur ‘Abbildung’ komplexer Bildfunktionen in der Elektronenmikroskopie by Hoppe (1971)-
Krakow W. , & Siegel B. M. (1972). Characteristics of an electrostatic phase plate. 30thAnn. Proc. EMSA, 618–19.
(
10.1017/S0424820100096345
) 10.1080/713826306
{'key': 'S0033583500001414_ref076', 'first-page': '607', 'article-title': "Un filtre de fréquénces spatiales pour l'amélioration du contraste des images optiques", 'volume': '237', 'author': 'Maréchal', 'journal-title': 'C. r. hebd. Seanc. Acad. Sci'}
/ C. r. hebd. Seanc. Acad. Sci / Un filtre de fréquénces spatiales pour l'amélioration du contraste des images optiques by Maréchal10.1016/0042-6822(63)90143-0
10.1126/science.177.4045.268
{'key': 'S0033583500001414_ref079', 'first-page': 'L6', 'article-title': 'A method for the solution of the phase problem in electron microscopy', 'volume': '6', 'author': 'Misell', 'year': '1973', 'journal-title': 'J. Phys.'}
/ J. Phys. / A method for the solution of the phase problem in electron microscopy by Misell (1973){'key': 'S0033583500001414_ref082', 'first-page': '287', 'article-title': 'Preparation of aluminium oxide films for high-resolution electron microscopy', 'volume': '35', 'author': 'Müller', 'year': '1972', 'journal-title': 'Optik'}
/ Optik / Preparation of aluminium oxide films for high-resolution electron microscopy by Müller (1972)-
Parsons D. F. , Matricardi V. R. , Moretz R. C. & Tuner J. N. (1973 a). Electron microscopy and diffraction of wet, unstained and unfixed biological objects. Adv. biol. med. Phys. 15 (in the Press).
(
10.1016/B978-0-12-005215-8.50012-7
) -
Parsons D. F. , Matricardi V. R. & Uydess I. (1973 b). High-voltage electron microscopy of wet whole cancer and normal cells. II. Influence of specimen preparation conditions. J. Microscopy (inthe ress.)
(
10.1016/0005-2736(72)90056-9
) {'key': 'S0033583500001414_ref088', 'first-page': '226', 'article-title': 'Thin pyrolytic graphite films for electron microscope substrates', 'author': 'Riddle', 'year': '1971', 'journal-title': '29th Ann. Proc. EMSA'}
/ 29th Ann. Proc. EMSA / Thin pyrolytic graphite films for electron microscope substrates by Riddle (1971){'key': 'S0033583500001414_ref090', 'first-page': '63', 'article-title': 'Phase determination in bright-field electron microscopy using complementary half-plane apertures', 'volume': '7', 'author': 'Saxton', 'year': '1974', 'journal-title': 'J. Phys'}
/ J. Phys / Phase determination in bright-field electron microscopy using complementary half-plane apertures by Saxton (1974)10.1016/S0022-5320(68)80065-6
{'key': 'S0033583500001414_ref091', 'first-page': '145', 'article-title': 'Zur Frage der Bildrekonstruktion durch Fokusreihen', 'volume': 'I', 'author': 'Schiske', 'year': '1968', 'journal-title': 'Proc. IVth Eur. Reg. Congr. Electron Microsc., Rome'}
/ Proc. IVth Eur. Reg. Congr. Electron Microsc., Rome / Zur Frage der Bildrekonstruktion durch Fokusreihen by Schiske (1968){'key': 'S0033583500001414_ref092', 'first-page': '82', 'volume-title': '‘Image processing and computer aided design’', 'author': 'Schiske', 'year': '1973'}
/ ‘Image processing and computer aided design’ by Schiske (1973){'key': 'S0033583500001414_ref057', 'first-page': '116', 'article-title': 'On the reconstruction of a weak phase-amplitude object: II', 'volume': '35', 'author': 'Hoenders', 'year': '1972', 'journal-title': 'Optik'}
/ Optik / On the reconstruction of a weak phase-amplitude object: II by Hoenders (1972)- Schwertfeger W. (1974). Zur Kleinwinkel streuung von mittelschnellen Elektronen beim Durchgang durch amorphe Festkörperschichten. Thesis, University of Tübingen.
10.1002/j.1538-7305.1964.tb01037.x
10.1038/238435a0
{'key': 'S0033583500001414_ref098', 'first-page': '516', 'article-title': 'Appendix of ‘A study of the structure of the T-layer of Bacillus brevis’', 'volume': 'II', 'author': 'Smith', 'year': '1973', 'journal-title': 'J. Supramol. Struct.'}
/ J. Supramol. Struct. / Appendix of ‘A study of the structure of the T-layer of Bacillus brevis’ by Smith (1973)10.1515/zna-1965-0129
/ Z. Naturf / Elektronenmikroskopische Untersuchungen an dünnen Kohlefolien by Thon (1965)10.1098/rspa.1972.0116
{'key': 'S0033583500001414_ref032', 'first-page': '317', 'article-title': 'Theory of a weak phase- amplitude object IV', 'volume': '39', 'author': 'Ferwerda', 'year': '1974', 'journal-title': 'Optik'}
/ Optik / Theory of a weak phase- amplitude object IV by Ferwerda (1974)- Voter W. A. (1973). Thesis: Duke University Physics Dept
-
Welton T. A. (1971). Computational correction of aberrations in electron microscopy. 29th Ann. Proc. EMSA, 94–5.
(
10.1017/S0424820100064529
) - Welton T. A. (1974). Electron-optical factors limiting resolution in transmission electron microscopy. (To be published.)
- Willasch D. (1973). Versuche zur Kontrastverbesserung in der Elektronenmikroskopie durch Heilfeldabbildung mittels Phasenplatten und Dunkelfeldabbildung hei hohlkegelförmiger Beleuchtung. Thesis. Tübingen.
10.1016/0014-4827(53)90202-X
{'key': 'S0033583500001414_ref093', 'first-page': '261', 'article-title': 'Ein- und Mehrdeutigkeit der Phasenbestimmung aus Bud und Beugungsfigur', 'volume': '40', 'author': 'Schiske', 'year': '1974', 'journal-title': 'Optik.'}
/ Optik. / Ein- und Mehrdeutigkeit der Phasenbestimmung aus Bud und Beugungsfigur by Schiske (1974)- Elliott A. & Offer J. (1974). Unpublished results.
{'key': 'S0033583500001414_ref058', 'first-page': '542', 'article-title': 'On the reconstruction of a weak phase-amplitude object: II', 'volume': '37', 'author': 'Hoenders', 'year': '1973', 'journal-title': 'Optik'}
/ Optik / On the reconstruction of a weak phase-amplitude object: II by Hoenders (1973)10.1111/j.1365-2818.1974.tb03940.x
{'key': 'S0033583500001414_ref022', 'first-page': '119', 'article-title': 'Electron adsorption of particles to the support film and its consequences on particle counts in electron microscopy,', 'volume': '72', 'author': 'Dubochet', 'year': '1972', 'journal-title': 'Microscopica Acta'}
/ Microscopica Acta / Electron adsorption of particles to the support film and its consequences on particle counts in electron microscopy, by Dubochet (1972)10.1016/S0021-9258(18)60301-1
/ J. biol. Chem / Effect of ethylene glycol on the conformation of γ-globulin and β-lactalbumin by Tanford (1962)
Dates
Type | When |
---|---|
Created | 16 years, 5 months ago (March 17, 2009, 12:19 p.m.) |
Deposited | 1 year, 5 months ago (March 11, 2024, 4:06 p.m.) |
Indexed | 4 weeks ago (July 25, 2025, 6:59 a.m.) |
Issued | 51 years, 3 months ago (May 1, 1974) |
Published | 51 years, 3 months ago (May 1, 1974) |
Published Online | 16 years, 5 months ago (March 17, 2009) |
Published Print | 51 years, 3 months ago (May 1, 1974) |
@article{Beer_1974, title={The possibilities and prospects of obtaining high-resolution information (below 30 Å) on biological material using the electron microscope: Some comments and reports inspired by an EMBO workshop held at Gais, Switzerland, October 1973}, volume={7}, ISSN={1469-8994}, url={http://dx.doi.org/10.1017/s0033583500001414}, DOI={10.1017/s0033583500001414}, number={2}, journal={Quarterly Reviews of Biophysics}, publisher={Cambridge University Press (CUP)}, author={Beer, M. and Frank, J. and Hanszen, K.-J. and Kellenberger, E. and Williams, R. C.}, year={1974}, month=may, pages={211–238} }