DOI: 10.1017/s0031182000056481. <i>In vitro</i> formation of ookinetes and functional maturity of <i>Plasmodium berghei</i> gametocytes

In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytes

10.1017/s0031182000056481

Crossref journal-article

Cambridge University Press (CUP)

Parasitology (56)

Abstract

In vitro formation of Plasmodium berghei ookinetes was studied. Gametocytes produced in vitro were obtained from heart and tail blood of Swiss mice and from blood removed from mosquitoes directly after feeding on these mice. In vitro produced gametocytes were obtained from short-term cultures of the erythrocytic stages of P. berghei. Reproducible ookinete production was obtained in medium RPMI 1640, pH 7°8–8°0, using in vivo and in vitro produced gametocytes. The morphology of developmental stages of ookinetes and degenerate forms at the light microscope level is described. More ookinetes were produced in medium RPMI 1640 compared to MEM and ookinete yield – defined as the ratio between the number of in vitro produced ookinetes/105 erythrocytes and the number of exflagellations/105 erythrocytes in the infected blood – increased with lower erythrocyte densities in the cultures within the range of dilutions tested. A linear relationship existed between gametocytaemia and the number of ookinetes produced. The methods for in vitro ookinete formation and for estimating ookinete yields enabled us to study aspects of functional maturity of gametocytes independent of mosquitoes. The numbers of exflagellating gametocytes and in vitro ookinete yields in tail blood corresponded with those in heart blood and blood ingested by mosquitoes, suggesting a random distribution of functionally mature gametocytes within the vertebrate host.

Bibliography

Janse, C. J., Mons, B., Rouwenhorst, R. J., van der Klooster, P. F. J., Overdulve, J. P., & van der Kaay, H. J. (1985). In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytes. Parasitology, 91(1), 19â29.

Authors 6

C. J. Janse (first)
B. Mons (additional)
R. J. Rouwenhorst (additional)
P. F. J. van der Klooster (additional)
J. P. Overdulve (additional)
H. J. van der Kaay (additional)

References 13 Referenced 92

10.1016/0014-4894(79)90056-0
{'key': 'S0031182000056481_ref008', 'article-title': 'Development of sexual and asexual stages of Plasmodium berghei in synchronous infections and in vitro cultures', 'author': 'Moss', 'year': '1985', 'journal-title': 'Parasitology'} / Parasitology / Development of sexual and asexual stages of Plasmodium berghei in synchronous infections and in vitro cultures by Moss (1985)
{'key': 'S0031182000056481_ref002', 'first-page': '21', 'volume-title': 'Abstracts of the 5th International Congress of Protozoology', 'author': 'Chen', 'year': '1977'} / Abstracts of the 5th International Congress of Protozoology by Chen (1977)
10.2307/3279917
{'key': 'S0031182000056481_ref011', 'first-page': '154', 'volume-title': 'Advances in Parasitology', 'author': 'Sinden', 'year': '1983'} / Advances in Parasitology by Sinden (1983)
10.1016/B978-0-12-426103-7.50013-6
{'key': 'S0031182000056481_ref012', 'first-page': '13', 'article-title': 'A susceptible and refractive strain of Anopheles atroparvus to infection with Plasmodium berghei berghei', 'volume': '45', 'author': 'Van der Kaay', 'year': '1977', 'journal-title': 'Acta Leidensia'} / Acta Leidensia / A susceptible and refractive strain of Anopheles atroparvus to infection with Plasmodium berghei berghei by Van der Kaay (1977)
{'key': 'S0031182000056481_ref003', 'first-page': '153', 'article-title': 'Morphological observations on the ookinete formation in rodent plasmodium (Plasmodiuin berghei yoelii) in vitro', 'volume': '27', 'author': 'Gao', 'year': '1981', 'journal-title': 'Acta Zoologica Sinica'} / Acta Zoologica Sinica / Morphological observations on the ookinete formation in rodent plasmodium (Plasmodiuin berghei yoelii) in vitro by Gao (1981)
10.1016/0020-7519(83)90015-2
{'key': 'S0031182000056481_ref004', 'first-page': '127', 'article-title': 'Influences of blood digestion on the development of Plasmodiuni gallinaceum (Brumpt) in the midgut of Aedes aegypti (L.)', 'volume': '34', 'author': 'Gass', 'year': '1977', 'journal-title': 'Acta tropica'} / Acta tropica / Influences of blood digestion on the development of Plasmodiuni gallinaceum (Brumpt) in the midgut of Aedes aegypti (L.) by Gass (1977)
{'key': 'S0031182000056481_ref005', 'first-page': '243', 'article-title': 'In vitro damage of cultured ookinetes of Plasmodium gallinaceum by digestive proteinases from susceptible', 'volume': '36', 'author': 'Gass', 'year': '1979', 'journal-title': 'Aedes aegypti Acta tropica'} / Aedes aegypti Acta tropica / In vitro damage of cultured ookinetes of Plasmodium gallinaceum by digestive proteinases from susceptible by Gass (1979)
10.1051/parasite/1979542145
10.1016/0020-7519(84)90083-3

Dates

Type	When
Created	16 years, 3 months ago (June 5, 2009, 8:23 a.m.)
Deposited	6 years, 3 months ago (May 22, 2019, 2:02 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 13, 2024, 11:53 a.m.)
Issued	40 years, 1 month ago (Aug. 1, 1985)
Published	40 years, 1 month ago (Aug. 1, 1985)
Published Online	16 years, 4 months ago (April 6, 2009)
Published Print	40 years, 1 month ago (Aug. 1, 1985)

Funders 0

None

BibTeX

@article{Janse_1985, title={In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytes}, volume={91}, ISSN={1469-8161}, url={http://dx.doi.org/10.1017/s0031182000056481}, DOI={10.1017/s0031182000056481}, number={1}, journal={Parasitology}, publisher={Cambridge University Press (CUP)}, author={Janse, C. J. and Mons, B. and Rouwenhorst, R. J. and van der Klooster, P. F. J. and Overdulve, J. P. and van der Kaay, H. J.}, year={1985}, month=aug, pages={19–29} }

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