DOI: 10.1017/s0022172400028539. Soluble antigens obtained from influenza virus by treatment with non-ionic detergent

Soluble antigens obtained from influenza virus by treatment with non-ionic detergent

10.1017/s0022172400028539

Crossref journal-article

Cambridge University Press (CUP)

Journal of Hygiene (56)

Abstract

SummaryHighly purified influenza virus was degraded using anionic and non-ionic detergents. Best results were obtained using the non-ionic detergent Triton N101. Tests showed that virus extracts contained neuraminidase and a substance that reacted specifically with rabbit antibody to virus haemagglutinin (specific serum blocking substance). Haemagglutination-inhibiting antibody was produced when virus extracts were inoculated into guinea-pigs. Immunodiffusion tests showed that extracts were complex. Host-specific material was regularly found. Under appropriate conditions S-antigen was detected as a single line pattern component. Two or more virus-specific materials were also present. One of these was probably neuraminidase and the other the specific serum blocking substance.

Bibliography

Corbel, M. J., & Rondle, C. J. M. (1970). Soluble antigens obtained from influenza virus by treatment with non-ionic detergent. Journal of Hygiene, 68(1), 81â96.

Authors 2

M. J. Corbel (first)
C. J. M. Rondle (additional)

References 47 Referenced 6

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10.4049/jimmunol.89.1.136 / Journal of Immunology / Evidence for the absence of anaphylactogenic host protein in highly purified PR 8 influenza virus by Kroeger (1962)
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{'key': 'S0022172400028539_ref006', 'first-page': '5', 'article-title': 'Comparison of serological and febrile responses in humans to vaccination with influenza A viruses or their haemagglutinins', 'volume': '63', 'author': 'Davenport', 'year': '1964', 'journal-title': 'Journal of Laboratory and Clinical Medicine'} / Journal of Laboratory and Clinical Medicine / Comparison of serological and febrile responses in humans to vaccination with influenza A viruses or their haemagglutinins by Davenport (1964)
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{'key': 'S0022172400028539_ref025', 'first-page': '17', 'article-title': 'Antigenic host components in the virus structure', 'volume': '10', 'author': 'Kosyakov', 'year': '1965', 'journal-title': 'Voprossy Virusologii'} / Voprossy Virusologii / Antigenic host components in the virus structure by Kosyakov (1965)
{'key': 'S0022172400028539_ref042', 'first-page': '208', 'article-title': 'Antibody response to natural influenza A2 infection of man as studied by gel double diffusion', 'volume': '12', 'author': 'Styk', 'year': '1968', 'journal-title': 'Acta Virologica'} / Acta Virologica / Antibody response to natural influenza A2 infection of man as studied by gel double diffusion by Styk (1968)
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10.1159/000228394

Dates

Type	When
Created	16 years, 3 months ago (May 15, 2009, 6:17 a.m.)
Deposited	6 months, 3 weeks ago (Feb. 9, 2025, 7:31 a.m.)
Indexed	6 months, 3 weeks ago (Feb. 9, 2025, 8:10 a.m.)
Issued	55 years, 6 months ago (March 1, 1970)
Published	55 years, 6 months ago (March 1, 1970)
Published Online	16 years, 3 months ago (May 15, 2009)
Published Print	55 years, 6 months ago (March 1, 1970)

Funders 0

None

BibTeX

@article{Corbel_1970, title={Soluble antigens obtained from influenza virus by treatment with non-ionic detergent}, volume={68}, ISSN={0022-1724}, url={http://dx.doi.org/10.1017/s0022172400028539}, DOI={10.1017/s0022172400028539}, number={1}, journal={Journal of Hygiene}, publisher={Cambridge University Press (CUP)}, author={Corbel, M. J. and Rondle, C. J. M.}, year={1970}, month=mar, pages={81–96} }

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  "alternative-id": [
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  "URL": "http://dx.doi.org/10.1017/S0022172400028539",
  "relation": {},
  "ISSN": [
    "0022-1724"
  ],
  "subject": [],
  "container-title-short": "J. Hyg.",
  "published": {
    "date-parts": [
      [
        1970,
        3
      ]
    ]
  },
  "assertion": [
    {
      "value": "Cambridge University Press 1970",
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        "name": "copyright_and_licensing",
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}