DOI: 10.1017/s002217240002458x. A surface antigen influenza vaccine. 1. Purification of haemagglutinin and neuraminidase proteins

A surface antigen influenza vaccine. 1. Purification of haemagglutinin and neuraminidase proteins

10.1017/s002217240002458x

Crossref journal-article

Cambridge University Press (CUP)

Journal of Hygiene (56)

Abstract

SummaryInfluenza virus was centrifuged in a KII rotor through a sucrose gradient containing Triton N101, a non-ionic surfactant. The micelles of surfactant formed a band in the gradient. As virus particles passed through the surfactant, the haemagglutinin and neuraminidase proteins were stripped from the surface and remained near the surfactant micelles. The residual virus particles sedimented into a denser region of the gradient and were thus separated from the haemagglutinin and neuraminidase antigens. Fractions containing the surface antigens were pooled and Triton was removed by phase-separation at the cloud point.

Bibliography

Brady, M. I., & Furminger, I. G. S. (1976). A surface antigen influenza vaccine. 1. Purification of haemagglutinin and neuraminidase proteins. Journal of Hygiene, 77(2), 161â172.

Authors 2

Margaret I. Brady (first)
I. G. S. Furminger (additional)

References 27 Referenced 39

10.1128/JVI.1.6.1207-1216.1967 / Journal of Virology / Purification of large quantities of influenza virus by density gradient centrifugation by Reimer (1967)
10.1136/pgmj.52.608.368
10.1038/2211255a0
10.1042/bj0340858
10.1139/o59-099
10.1128/JVI.2.8.778-786.1968 / Journal of Virology / Protective effects of specific immunity to viral neuraminidase on influenza virus infection of mice by Schulman (1968)
10.1016/0042-6822(71)90271-6
{'key': 'S002217240002458X_ref010', 'first-page': '5', 'article-title': 'Comparisons of serologic and febrile responses in humans to vaccination with influenza A viruses or their haemagglutinins', 'volume': '64', 'author': 'Davenport', 'year': '1964', 'journal-title': 'Journal of Laboratory and Clinical Medicine'} / Journal of Laboratory and Clinical Medicine / Comparisons of serologic and febrile responses in humans to vaccination with influenza A viruses or their haemagglutinins by Davenport (1964)
10.1016/S0021-9258(18)69851-5 / Journal of Biological Chemistry / The thiobarbituric acid assay of sialic acids by Warren (1959)
{'key': 'S002217240002458X_ref018', 'first-page': '145', 'article-title': 'The effects of non-aqueous solvents on the quaternary structure of viruses: a procedure for the simultaneous concentration, purification and disruption of influenza viruses', 'volume': '4', 'author': 'Neurath', 'year': '1971', 'journal-title': 'Microbios'} / Microbios / The effects of non-aqueous solvents on the quaternary structure of viruses: a procedure for the simultaneous concentration, purification and disruption of influenza viruses by Neurath (1971)
10.1017/S0022172400028527
10.1017/S0022172400024591
{'key': 'S002217240002458X_ref017', 'volume-title': 'Lancet i', 'author': 'Monto', 'year': '1973'} / Lancet i by Monto (1973)
10.1093/infdis/129.4.411
10.1001/jama.1968.03150100027005
{'key': 'S002217240002458X_ref004', 'first-page': '167', 'article-title': 'The protection of ferrets against influenza by immunisation with a split influenza vaccine. International Symposium on Immunity to Infections of the Respiratory System', 'volume': '28', 'author': 'Brady', 'year': '1975', 'journal-title': 'Developments of Biological Standardisation'} / Developments of Biological Standardisation / The protection of ferrets against influenza by immunisation with a split influenza vaccine. International Symposium on Immunity to Infections of the Respiratory System by Brady (1975)
10.4049/jimmunol.96.4.596 / Journal of Immunology / Influenza subunit vaccines: immunogenicity and lack of toxicity for rabbits of ether and detergent-disrupted virus by Webster (1966)
10.1099/0022-1317-4-3-355
10.1017/S0022172400021847
10.1017/S0022172400022610
10.1093/infdis/124.5.449
10.1016/0042-6822(61)90276-8
10.1016/S0021-9258(19)52451-6 / Journal of Biological Chemistry / Protein measurement with the Folin-phenol reagent by Lowry (1951)
10.1001/jama.1969.03160340046009
10.1099/0022-1317-16-2-231
10.1038/newbio238145a0
10.1017/S0022172400024402

Dates

Type	When
Created	16 years, 3 months ago (May 15, 2009, 5:51 a.m.)
Deposited	1 year, 5 months ago (March 13, 2024, 7:12 a.m.)
Indexed	1 year, 5 months ago (March 13, 2024, 7:40 a.m.)
Issued	48 years, 10 months ago (Oct. 1, 1976)
Published	48 years, 10 months ago (Oct. 1, 1976)
Published Online	16 years, 3 months ago (May 15, 2009)
Published Print	48 years, 10 months ago (Oct. 1, 1976)

Funders 0

None

BibTeX

@article{Brady_1976, title={A surface antigen influenza vaccine. 1. Purification of haemagglutinin and neuraminidase proteins}, volume={77}, ISSN={0022-1724}, url={http://dx.doi.org/10.1017/s002217240002458x}, DOI={10.1017/s002217240002458x}, number={2}, journal={Journal of Hygiene}, publisher={Cambridge University Press (CUP)}, author={Brady, Margaret I. and Furminger, I. G. S.}, year={1976}, month=oct, pages={161–172} }

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