DOI: 10.1017/s0022029900010955. The reactivation of milk alkaline phosphatase after heat treatment

The reactivation of milk alkaline phosphatase after heat treatment

10.1017/s0022029900010955

Crossref journal-article

Cambridge University Press (CUP)

Journal of Dairy Research (56)

Abstract

SummaryA simple system was developed, consisting of a solution of β-glycerophosphate, β-lactoglobulin and magnesium ions, in which alkaline phosphatase isolated from milk became strongly reactivated following heat treatment for 45 sec in boiling water. From 10 to 30% of the original enzyme activity reappeared after incubation at 37°C. Variations in the components of the system, and factors affecting it, were studied. Salts of Hg, Zn and Cd inhibited reactivation at low concentrations.Milk which, after the same heat treatment, became reactivated to a much smaller extent (about 1%), was found to contain a dialysable, heat labile inhibitor whose presence is thought to be largely responsible for the low level of reactivation, though other factors, e.g. the suboptimal concentration of phosphate esters must be considered as contributory causes.Milk contains also a non-dialysable, heat stable activator of the reactivation process, capable of replacing β-lactoglobulin in the simple system and active at very low concentration, e.g. 0·2% (v/v).

Bibliography

Lyster, R. L. J., & Aschaffenburg, R. (1962). The reactivation of milk alkaline phosphatase after heat treatment. Journal of Dairy Research, 29(1), 21â35.

Authors 2

R. L. J. Lyster (first)
R. Aschaffenburg (additional)

References 19 Referenced 25

10.1042/bj0550795
10.1017/S0022029900006828
10.1021/ja01132a041
10.3168/jds.S0022-0302(57)94695-7
10.1017/S0022029900007111
10.1016/0003-9861(54)90093-4
10.1017/S0022029900006919
10.1042/bj0650273
10.3168/jds.S0022-0302(57)94436-3
10.1016/0003-9861(52)90277-4
10.1017/S0022029900008268
{'key': 'S0022029900010955_ref007', 'first-page': '1018', 'volume': '3', 'author': 'Fuchs', 'year': '1953', 'journal-title': 'Proc. 13th Int. Dairy Congr.'} / Proc. 13th Int. Dairy Congr. by Fuchs (1953)
10.1017/S0022029900005756
Andersen K. P. , Møller-Madsen A. , Wittig G. & Faxholm H. (1948). Beretn. Forsøgsm. Kbh. no. 57.
{'key': 'S0022029900010955_ref011', 'first-page': '414', 'volume': '43', 'author': 'McFarren', 'year': '1960', 'journal-title': 'J. Ass. off. agric. Chem.'} / J. Ass. off. agric. Chem. by McFarren (1960)
10.1017/S0022029900005288
10.3168/jds.S0022-0302(58)91152-4
{'key': 'S0022029900010955_ref004', 'volume-title': 'The Enzymes', 'author': 'Dixon', 'year': '1958'} / The Enzymes by Dixon (1958)
10.1042/bj0550786

Dates

Type	When
Created	16 years, 3 months ago (June 5, 2009, 7:51 a.m.)
Deposited	6 years, 3 months ago (June 4, 2019, 4:04 p.m.)
Indexed	1 year, 10 months ago (Nov. 4, 2023, 2:42 p.m.)
Issued	63 years, 7 months ago (Feb. 1, 1962)
Published	63 years, 7 months ago (Feb. 1, 1962)
Published Online	16 years, 3 months ago (June 1, 2009)
Published Print	63 years, 7 months ago (Feb. 1, 1962)

Funders 0

None

BibTeX

@article{Lyster_1962, title={The reactivation of milk alkaline phosphatase after heat treatment}, volume={29}, ISSN={1469-7629}, url={http://dx.doi.org/10.1017/s0022029900010955}, DOI={10.1017/s0022029900010955}, number={1}, journal={Journal of Dairy Research}, publisher={Cambridge University Press (CUP)}, author={Lyster, R. L. J. and Aschaffenburg, R.}, year={1962}, month=feb, pages={21–35} }

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