DOI: 10.1017/s0022029900005288. 381. A rapid and simple phosphatase test for milk

381. A rapid and simple phosphatase test for milk

10.1017/s0022029900005288

Crossref journal-article

Cambridge University Press (CUP)

Journal of Dairy Research (56)

Abstract

A simple and rapid phosphatase test is described which is based on the use ofp–nitrophenylphosphate as the substrate. Execution of the test requires few and easy operations: incubation of the milk at 37° C. with a buffered solution of the substrate, followed directly by a visual comparison of the intensity of yellow colour of liberatedp–nitrophenol with that of boiled-milk standards containing known amounts ofp–nitrophenol. The test permits the detection of minor processing faults with an incubation time of only 30 min. In this form it should prove of value in creamery control. A more searching, but equally simple test is obtained by increasing the period of incubation to 2 hr.

Bibliography

Aschaffenburg, R., & Mullen, J. E. C. (1949). 381. A rapid and simple phosphatase test for milk. Journal of Dairy Research, 16(1), 58â67.

Authors 2

R. Aschaffenburg (first)
J. E. C. Mullen (additional)

References 11 Referenced 68

(7) Hartmann S. & Andersen K. (1942–1943). Beretn. Forsoksm., Kbh., No. 38, p. 15. (10.1259/0007-1285-15-170-38)
10.1016/S0021-9258(17)35142-6 / J. biol. Chem. by Axelrod (1947)
10.1017/S0022029900004921
10.1017/S0022029900003095
{'key': 'S0022029900005288_ref010', 'first-page': '278', 'volume': '8', 'author': 'King', 'year': '1940', 'journal-title': 'Enzymologia'} / Enzymologia by King (1940)
10.1016/S0021-9258(19)52801-0 / J. biol. Chem. by Huggins (1945)
10.1016/S0021-9258(18)43072-4 / J. biol. Chem. by Bessey (1946)
10.1017/S0022029900001333
{'key': 'S0022029900005288_ref011', 'first-page': '129', 'volume': '29', 'author': 'Horwitz', 'year': '1946', 'journal-title': 'J. Ass. off. agric. Chem., Wash.'} / J. Ass. off. agric. Chem., Wash. by Horwitz (1946)
10.3168/jds.S0022-0302(38)95608-5
{'key': 'S0022029900005288_ref008', 'first-page': '217', 'volume': '4', 'author': 'Ohmori', 'year': '1937', 'journal-title': 'Enzymologia'} / Enzymologia by Ohmori (1937)

Dates

Type	When
Created	16 years, 3 months ago (June 5, 2009, 8:29 a.m.)
Deposited	3 years, 10 months ago (Oct. 6, 2021, 6:44 p.m.)
Indexed	1 week, 1 day ago (Aug. 29, 2025, 6:01 a.m.)
Issued	76 years, 8 months ago (Jan. 1, 1949)
Published	76 years, 8 months ago (Jan. 1, 1949)
Published Online	16 years, 3 months ago (June 1, 2009)
Published Print	76 years, 8 months ago (Jan. 1, 1949)

Funders 0

None

BibTeX

@article{Aschaffenburg_1949, title={381. A rapid and simple phosphatase test for milk}, volume={16}, ISSN={1469-7629}, url={http://dx.doi.org/10.1017/s0022029900005288}, DOI={10.1017/s0022029900005288}, number={1}, journal={Journal of Dairy Research}, publisher={Cambridge University Press (CUP)}, author={Aschaffenburg, R. and Mullen, J. E. C.}, year={1949}, month=jan, pages={58–67} }

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