DOI: 10.1017/s0016672300030135. Effect of ecd1 mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

Effect of ecd1 mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

10.1017/s0016672300030135

Crossref journal-article

Hindawi Limited

Genetical Research (98)

Abstract

SummaryThe Drosophila melanogasterecd1 mutation causes a severe temperature-sensitive deficiency in the titre of the steroid hormone eedysone. This mutation was used to investigate the role of eedysone in both the transcription of the genes mapped at the 3C11–12 intermoult puff region and the puff formation. Thoroughly synchronized ecd1 larvae were shifted to the non-permissive temperature at various times of the development; after 24 or 48 h, the levels of the transcripts derived from Sgs-4, Pig-1 and ng-1, the three genes located at the 3C11–12 polytene bands, were determined. The results showed that the levels of the transcripts encoded by Pig-1 and ng-1 are unaffected by the drop in the ecdysone titre occurring in non-permissive conditions whereas the amount of Sgs-4 mRNA is greatly reduced. These data clearly indicate that transcription of the three genes mapped within the puff region is affected differently by the hormone. Furthermore, ecd1 larvae cultured at the non-permissive temperature show a prominent puff at the 3C11–12 polytene bands, indicating that eedysone is not essential for puff induction and that puff size is not simply correlated with high-level Sgs-4 transcription.

Bibliography

Furia, M., Dâavino, P. P., Digilio, F. A., Crispi, S., Giordano, E., & Polito, L. C. (1992). Effect of ecd1 mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff. Genetical Research, 59(1), 19â26.

Authors 6

Maria Furia (first)
Pier Paolo D'avino (additional)
Filomena A. Digilio (additional)
Stefania Crispi (additional)
Ennio Giordano (additional)
Lino C. Polito (additional)

References 30 Referenced 8

10.1093/nar/12.13.5495
10.1073/pnas.77.12.7362
10.1073/pnas.80.4.1063
{'key': 'S0016672300030135_ref019', 'volume-title': 'Molecular Cloning', 'author': 'Maniatis', 'year': '1982'} / Molecular Cloning by Maniatis (1982)
{'key': 'S0016672300030135_ref018', 'first-page': '324', 'article-title': 'Promoter is an important determinant of Cellularityly regulated puffing at the Sgs-4 locus of Drosophila melanogaster', 'volume': '138', 'author': 'Korge', 'year': '1990', 'journal-title': 'Cellularity Biology'} / Cellularity Biology / Promoter is an important determinant of Cellularityly regulated puffing at the Sgs-4 locus of Drosophila melanogaster by Korge (1990)
10.1002/j.1460-2075.1983.tb01587.x / EMBO Journal / Puffing activities and binding of ecdysteroid to polytene chromosomes of Drosophila melanogaster by Dworniczak (1983)
10.1007/BF00399132
{'key': 'S0016672300030135_ref002', 'first-page': '1', 'volume-title': 'The Genetics and Biology of Drosophila', 'volume': '2', 'author': 'Ashburner', 'year': '1978'} / The Genetics and Biology of Drosophila by Ashburner (1978)
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{'key': 'S0016672300030135_ref001', 'first-page': '319', 'volume-title': 'The Genetics and Biology of Drosophila', 'volume': '2', 'author': 'Ashburner', 'year': '1978'} / The Genetics and Biology of Drosophila by Ashburner (1978)
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10.1016/0303-7207(81)90013-7

Dates

Type	When
Created	15 years, 11 months ago (Sept. 17, 2009, 3:28 a.m.)
Deposited	4 years, 8 months ago (Dec. 31, 2020, 5:46 p.m.)
Indexed	1 year, 11 months ago (Sept. 7, 2023, 7:27 a.m.)
Issued	33 years, 7 months ago (Feb. 1, 1992)
Published	33 years, 7 months ago (Feb. 1, 1992)
Published Online	16 years, 4 months ago (April 14, 2009)
Published Print	33 years, 7 months ago (Feb. 1, 1992)

Funders 0

None

BibTeX

@article{Furia_1992, title={Effect of ecd1 mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff}, volume={59}, ISSN={1469-5073}, url={http://dx.doi.org/10.1017/s0016672300030135}, DOI={10.1017/s0016672300030135}, number={1}, journal={Genetical Research}, publisher={Hindawi Limited}, author={Furia, Maria and D’avino, Pier Paolo and Digilio, Filomena A. and Crispi, Stefania and Giordano, Ennio and Polito, Lino C.}, year={1992}, month=feb, pages={19–26} }

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