DOI: 10.1017/s0016672300011174. Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controlling resistance

Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controlling resistance

10.1017/s0016672300011174

Crossref journal-article

Hindawi Limited

Genetical Research (98)

Abstract

Erythromycin-resistant mutants were separated into two categories showing Mendelian and non-Mendelian inheritance of resistance respectively. The cytoplasmic factor was located in the mitochondrion based on the fact that the loss of the mitochondrial genetic unit associated with the petite mutation (the ρ factor, assumed to be the mitochondrial DNA) resulted in a corresponding permanent loss of resistance. Conversely, the petite mutation in gene resistant mutants had no effect on the inheritance of resistance.Analysis of vegetative and sexual progeny of isolated zygotes from a number of crosses between resistant and sensitive strains indicated that different mitochondrial types, both sensitive and resistant, co-exist in the yeast cell and are distributed at random among daughter cells under non-selective conditions. Only resistant mitochondria are in evidence if zygotes are put down to proliferate on medium containing the antibiotic.Resistant strains were tested for retention of resistance following anaerobic culture (known to cause disruption of mitochondria). The cytoplasmic mutants retained their resistance but two gene-resistant mutants tested both lost their resistance. It was suggested in the latter case that resistance is dependent on the existence of intact mitochondrial membrane (possibly inner membrane). In the former mutants the mechanism of resistance may be the alteration of one or other of the components of the protein-synthesizing system of the organelle.

Bibliography

Thomas, D. Y., & Wilkie, D. (1968). Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controlling resistance. Genetical Research, 11(1), 33â41.

Authors 2

D. Y. Thomas (first)
D. Wilkie (additional)

References 24 Referenced 110

{'key': 'S0016672300011174_ref020', 'volume-title': 'Biochemical Aspects of the Biogenesis of Mitochondria', 'author': 'Wilkie', 'year': '1967'} / Biochemical Aspects of the Biogenesis of Mitochondria by Wilkie (1967)
10.1016/0304-4165(64)90113-8
{'key': 'S0016672300011174_ref013', 'volume-title': 'The Biogenesis of Mitochondria', 'author': 'Roodyn', 'year': '1967'} / The Biogenesis of Mitochondria by Roodyn (1967)
10.1016/0006-291X(66)90723-6
10.1016/0006-291X(66)90734-0
{'key': 'S0016672300011174_ref016', 'volume-title': 'Formation des enzymes respiratoires chez la levure', 'author': 'Slonimski', 'year': '1953'} / Formation des enzymes respiratoires chez la levure by Slonimski (1953)
{'key': 'S0016672300011174_ref008', 'volume-title': 'Biochemical Aspects of the Biogenesis of Mitochondria', 'author': 'Linnane', 'year': '1967'} / Biochemical Aspects of the Biogenesis of Mitochondria by Linnane (1967)
10.1073/pnas.55.4.872
{'key': 'S0016672300011174_ref006', 'volume-title': 'Nucleo-cytoplasmic relationships in micro-organisms', 'author': 'Ephrussi', 'year': '1953'} / Nucleo-cytoplasmic relationships in micro-organisms by Ephrussi (1953)
{'key': 'S0016672300011174_ref001', 'volume-title': 'Teaching Genetics', 'author': 'Beavan', 'year': '1964'} / Teaching Genetics by Beavan (1964)
10.1093/genetics/45.8.1085 / Genetics / Chromosome mapping in Saccharomyces by Hawthorne (1960)
10.1083/jcb.34.1.1
10.1038/176022a0
10.1126/science.151.3711.687
10.1016/0022-2836(67)90302-6
10.1016/0006-291X(66)90631-0
10.1016/S0022-5320(62)80031-8
10.1016/0005-2787(66)90309-1
10.1016/0022-2836(66)90003-9
10.1017/S0016672300010934
10.1126/science.156.3779.1234
{'key': 'S0016672300011174_ref022', 'first-page': '409', 'article-title': 'Proteinsynthese in isolierten Hefe-Mitochondrien', 'volume': '341', 'author': 'Wintersberger', 'year': '1965', 'journal-title': 'Biochem. Z.'} / Biochem. Z. / Proteinsynthese in isolierten Hefe-Mitochondrien by Wintersberger (1965)
10.1042/bj1030003C
10.1016/0006-291X(66)90268-3

Dates

Type	When
Created	15 years, 11 months ago (Sept. 17, 2009, 3:56 a.m.)
Deposited	3 years, 10 months ago (Oct. 10, 2021, 6:53 p.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 10:38 a.m.)
Issued	57 years, 7 months ago (Feb. 1, 1968)
Published	57 years, 7 months ago (Feb. 1, 1968)
Published Online	16 years, 4 months ago (April 14, 2009)
Published Print	57 years, 7 months ago (Feb. 1, 1968)

Funders 0

None

BibTeX

@article{Thomas_1968, title={Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controlling resistance}, volume={11}, ISSN={1469-5073}, url={http://dx.doi.org/10.1017/s0016672300011174}, DOI={10.1017/s0016672300011174}, number={1}, journal={Genetical Research}, publisher={Hindawi Limited}, author={Thomas, D. Y. and Wilkie, D.}, year={1968}, month=feb, pages={33–41} }

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