DOI: 10.1016/j.tibtech.2004.08.012. High-throughput cell-free systems for synthesis of functionally active proteins

High-throughput cell-free systems for synthesis of functionally active proteins

10.1016/j.tibtech.2004.08.012

Crossref journal-article

Elsevier BV

Trends in Biotechnology (78)

Bibliography

Spirin, A. S. (2004). High-throughput cell-free systems for synthesis of functionally active proteins. Trends in Biotechnology, 22(10), 538â545.

Authors 1

Alexander S. Spirin (first)

References 76 Referenced 176

{'key': '10.1016/j.tibtech.2004.08.012_bib1', 'series-title': 'Cell-Free Translation Systems', 'first-page': '3', 'article-title': 'Cell-free protein synthesis', 'author': 'Spirin', 'year': '2002'} / Cell-Free Translation Systems / Cell-free protein synthesis by Spirin (2002)
10.1073/pnas.47.10.1588 / Proc. Natl. Acad. Sci. U. S. A. / The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polynucleotides by Nirenberg (1961)
10.1073/pnas.57.4.1010 / Proc. Natl. Acad. Sci. U. S. A. / DNA-directed peptide synthesis II. The synthesis of the α-fragment of the enzyme β-galactosidase by DeVries (1967)
10.1038/90802 / Nat. Biotechnol. / Cell-free translation reconstituted from purified components by Shimizu (2001)
10.1126/science.3055301 / Science / A continuous cell-free translation system capable of producing polypeptides in high yield by Spirin (1988)
10.1016/0378-1119(89)90521-0 / Gene / Gene expression in a cell-free system on the preparative scale by Baranov (1989)
10.1093/nar/17.11.4412 / Nucleic Acids Res. / Preparative synthesis of globin in a continuous cell-free translation system from rabbit reticulocytes by Ryabova (1989)
{'key': '10.1016/j.tibtech.2004.08.012_bib8', 'series-title': 'Frontiers in Bioprocessing II', 'first-page': '31', 'article-title': 'Cell-free protein synthesis bioreactor', 'author': 'Spirin', 'year': '1992'} / Frontiers in Bioprocessing II / Cell-free protein synthesis bioreactor by Spirin (1992)
10.1016/0076-6879(93)17059-E / Methods Enzymol. / Gene expression in cell-free systems on preparative scale by Baranov (1993)
10.1093/oxfordjournals.jbchem.a123551 / J. Biochem. / A continuous cell-free protein synthesis system for coupled transcription-translation by Kigawa (1991)
10.1016/0168-1656(92)90157-5 / J. Biotechnol. / Production of an enzymatic active protein using a continuous flow cell-free system by Endo (1992)
10.1016/0003-2697(92)90383-I / Anal. Biochem. / High efficiency cell-free synthesis of proteins: refinement of the coupled transcription/translation system by Kudlicki (1992)
10.1111/j.1470-8744.1992.tb00217.x / Biotechnol. Appl. Biochem. / Preparative in vitro synthesis of bioactive human interleukin-2 in a continuous flow translation system by Kolosov (1992)
10.1006/abio.1993.1490 / Anal. Biochem. / Synthesis of preparative amounts of biologically active interleukin-6 using a continuous-flow cell-free translation system by Volyanik (1993)
{'key': '10.1016/j.tibtech.2004.08.012_bib15', 'series-title': 'Cell-Free Translation Systems', 'first-page': '91', 'article-title': 'Continuous-flow and continuous-exchange cell-free translation systems and reactors', 'author': 'Shirokov', 'year': '2002'} / Cell-Free Translation Systems / Continuous-flow and continuous-exchange cell-free translation systems and reactors by Shirokov (2002)
10.1021/bp960052l / Biotechnol. Prog. / A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane by Kim (1996)
10.1016/S0014-5793(98)01620-2 / FEBS Lett. / Cell-free production and stable-isotope labelling of milligram quantities of proteins by Kigawa (1999)
10.1073/pnas.97.2.559 / Proc. Natl. Acad. Sci. U. S. A. / A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomes by Madin (2000)
10.1073/pnas.232580399 / Proc. Natl. Acad. Sci. U. S. A. / A cell-free protein synthesis system for high-throughput proteomics by Sawasaki (2002)
10.1023/B:JSFG.0000029208.83739.49 / J. Struct. Funct. Genomics / High-throughput, genome scale protein production method based on the wheat germ cell-free expression system by Endo (2004)
10.2144/01314pf01 / Biotechniques / High-yield, in vitro protein expression using a continuous-exchange, coupled transcription/translation system by Martin (2001)
10.2174/1389203033380359 / Curr. Protein Pept. Sci. / Rapid Translation System (RTS): A promising alternative for recombinant protein production by Betton (2003)
10.1002/(SICI)1097-0290(19990720)64:2<194::AID-BIT8>3.0.CO;2-5 / Biotechnol. Bioeng. / Efficient coupled transcription/translation for PCR template by a hollow fiber membrane bioreactor by Nakano (1999)
{'key': '10.1016/j.tibtech.2004.08.012_bib24', 'series-title': 'Cell-Free Translation Systems', 'first-page': '121', 'article-title': 'Matrix reactor: A new scalable principle for cell-free protein expression', 'author': 'Buchberger', 'year': '2002'} / Cell-Free Translation Systems / Matrix reactor: A new scalable principle for cell-free protein expression by Buchberger (2002)
10.1016/S0014-5793(02)02329-3 / FEBS Lett. / A bilayer cell-free protein synthesis system for high-throughout screening of gene products by Sawasaki (2002)
10.1074/mcp.T200004-MCP200 / Mol. Cell. Proteomics / An improved protein bioreactor. Efficient product isolation during in vitro protein biosynthesis via affinity tag by Lamla (2002)
10.1111/j.1432-1033.1996.0881u.x / Eur. J. Biochem. / A highly efficient cell-free protein synthesis system from Escherichia coli by Kim (1996)
10.1021/bp000031y / Biotechnol. Prog. / Prolonging cell-free protein synthesis by selective reagent additions by Kim (2000)
Michel-Reydellet, N. et al. Amino acid stabilization for cell-free protein synthesis by modification of the E. coli genome. Metab. Eng. (in press) (10.1016/j.ymben.2004.01.003)
10.1006/abio.1995.1208 / Anal. Biochem. / Acetyl phosphate as an energy source for bacterial cell-free translation systems by Ryabova (1995)
10.1002/(SICI)1097-0290(1999)66:3<180::AID-BIT6>3.0.CO;2-S / Biotechnol. Bioeng. / Prolonging cell-free protein synthesis with a novel ATP regeneration system by Kim (1999)
10.1002/bit.1121 / Biotechnol. Bioeng. / Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis by Kim (2001)
10.1002/bit.20026 / Biotechnol. Bioeng. / Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis by Jewett (2004)
10.1021/bp0341693 / Biotechnol. Prog. / Rapid expression and purification of 100nmol quantities of active protein using cell-free protein synthesis by Jewett (2004)
10.1111/j.1432-1033.1976.tb10656.x / Eur. J. Biochem. / An efficient mRNA-dependent translation system from reticulocyte lysates by Pelham (1976)
10.1002/j.1460-2075.1994.tb06670.x / EMBO J. / Folding of firefly luciferase during translation in a cell-free system by Kolb (1994)
10.1074/jbc.M002030200 / J. Biol. Chem. / Co-translational folding of an eukaryotic multidomain protein in a prokaryotic cell-free system by Kolb (2000)
10.1038/nbt0197-79 / Nat. Biotechnol. / Functional antibody production using cell-free translation: Effect of protein disulfide isomerase and chaperones by Ryabova (1997)
10.1016/S0014-5793(02)02383-9 / FEBS Lett. / Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation system by Jiang (2002)
10.1093/protein/gzg054 / Protein Eng. / Improvement of H2O2 stability of manganese peroxidase by combinatorial mutagenesis and high throughput screening using in vitro expression with protein disulfide isomerase by Miyasaki-Imamura (2003)
10.1002/bit.10827 / Biotechnol. Bioeng. / Enhancing multiple disulfide bonded protein folding in a cell-free system by Yin (2004)
10.1016/S0014-5793(04)00025-0 / FEBS Lett. / Cell-free production of active E. coli thioredoxin reductase and glutathione reductase by Knapp (2004)
10.1074/jbc.272.16.10646 / J. Biol. Chem. / Cotranslational folding of globin by Komar (1997)
{'key': '10.1016/j.tibtech.2004.08.012_bib44_1', 'series-title': 'Cell-Free Protein Expression', 'article-title': 'Optimization of cell-free expression of FAD-dependent D-amino acid oxidase', 'author': 'Lehmann', 'year': '2003'} / Cell-Free Protein Expression / Optimization of cell-free expression of FAD-dependent D-amino acid oxidase by Lehmann (2003)
10.1002/bit.10865 / Biotechnol. Bioeng. / Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts of Escherichia coli by Kim (2004)
Yang, J. et al. Expression of active murine granulocyte-macrophage colony-stimulating factor in an Escherichia coli cell-free system. Biotechnol. Prog. (in press) (10.1021/bp034350b)
{'key': '10.1016/j.tibtech.2004.08.012_bib47', 'series-title': 'Cell-Free Translation Systems', 'first-page': '149', 'article-title': 'Expression of soluble murine endostatin with the RTS system', 'author': 'Traviglia', 'year': '2000'} / Cell-Free Translation Systems / Expression of soluble murine endostatin with the RTS system by Traviglia (2000)
{'key': '10.1016/j.tibtech.2004.08.012_bib48', 'series-title': 'Cell-Free Translation Systems', 'first-page': '157', 'article-title': 'Cell-free expression of soluble human erythropoietin', 'author': 'Nemetz', 'year': '2000'} / Cell-Free Translation Systems / Cell-free expression of soluble human erythropoietin by Nemetz (2000)
{'key': '10.1016/j.tibtech.2004.08.012_bib49', 'series-title': 'Cell-Free Translation Systems', 'first-page': '165', 'article-title': 'Cell-free expression of a 127kDa protein: the catalytic subunit of human telomerase', 'author': 'Steigerwald', 'year': '2000'} / Cell-Free Translation Systems / Cell-free expression of a 127kDa protein: the catalytic subunit of human telomerase by Steigerwald (2000)
{'key': '10.1016/j.tibtech.2004.08.012_bib50', 'series-title': 'Cell-Free Translation Systems', 'first-page': '175', 'article-title': 'The expression of disulfide bonded proteins in cell-free protein expression', 'author': 'Fernholz', 'year': '2000'} / Cell-Free Translation Systems / The expression of disulfide bonded proteins in cell-free protein expression by Fernholz (2000)
{'key': '10.1016/j.tibtech.2004.08.012_bib51', 'series-title': 'Cell-Free Protein Expression', 'first-page': '125', 'article-title': 'Cell-free expression of proteins containing multiple disulfide bonds', 'author': 'Kim', 'year': '2003'} / Cell-Free Protein Expression / Cell-free expression of proteins containing multiple disulfide bonds by Kim (2003)
{'key': '10.1016/j.tibtech.2004.08.012_bib52_1', 'series-title': 'Cell-Free Protein Expression', 'article-title': 'Cell-free synthesis of membrane proteins on a preparative scale', 'author': 'Maurer', 'year': '2003'} / Cell-Free Protein Expression / Cell-free synthesis of membrane proteins on a preparative scale by Maurer (2003)
{'key': '10.1016/j.tibtech.2004.08.012_bib53', 'series-title': 'Cell-Free Protein Expression', 'first-page': '109', 'article-title': 'Screening and increasing soluble expression of recombinant proteins using the RTS 100 E. coli HY in a 96-well format', 'author': 'Busso', 'year': '2003'} / Cell-Free Protein Expression / Screening and increasing soluble expression of recombinant proteins using the RTS 100 E. coli HY in a 96-well format by Busso (2003)
10.1111/j.1432-1033.2003.03959.x / Eur. J. Biochem. / High level cell-free expression and specific labeling of integral membrane proteins by Klammt (2004)
10.1016/S0021-9258(18)52291-2 / J. Biol. Chem. / Use of in vitro synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies by Lesley (1991)
10.1016/S0014-5793(97)01011-9 / FEBS Lett. / Direct expression of PCR products in a cell-free transcription/translation system: Synthesis of antibacterial peptide cecropin by Martemyanov (1997)
10.1093/nar/26.19.4339 / Nucleic Acids Res. / In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation by Ohuchi (1998)
10.1016/S0022-2836(02)00094-3 / J. Mol. Biol. / High-throughput, cloning independent protein library construction by combining single-molecule DNA amplification with in vitro expression by Rungpragayphan (2002)
10.1016/S0014-5793(03)00251-5 / FEBS Lett. / PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries by Rungpragayphan (2003)
10.1016/S1367-5931(02)00019-4 / Curr. Opin. Chem. Biol. / Protein expression systems for structural genomics and proteomics by Yokoyama (2003)
10.1016/S0165-022X(03)00049-6 / J. Biochem. Biophys. Methods / Expression of soluble recombinant proteins in a cell-free system using a 96-well format by Busso (2003)
{'key': '10.1016/j.tibtech.2004.08.012_bib62', 'series-title': 'Cell-Free Protein Expression', 'first-page': '15', 'article-title': 'Expression-PCR: from gene pools to purified proteins within 1 day', 'author': 'Merk', 'year': '2003'} / Cell-Free Protein Expression / Expression-PCR: from gene pools to purified proteins within 1 day by Merk (2003)
10.1093/protein/15.2.147 / Protein Eng. / Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate-binding site by Yang (2002)
10.1016/S0022-2836(03)00782-4 / J. Mol. Biol. / Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high throughput screening using single-molecule PCR and in vitro expression by Koga (2003)
{'key': '10.1016/j.tibtech.2004.08.012_bib65', 'series-title': 'Cell-Free Translation Systems', 'first-page': '219', 'article-title': 'Selective labeling of proteins in the RTS 500 system', 'author': 'Betton', 'year': '2002'} / Cell-Free Translation Systems / Selective labeling of proteins in the RTS 500 system by Betton (2002)
{'key': '10.1016/j.tibtech.2004.08.012_bib66', 'series-title': 'Cell-Free Translation Systems', 'first-page': '227', 'article-title': 'In vitro protein production for structure determination with the RTS system', 'author': 'Cho', 'year': '2002'} / Cell-Free Translation Systems / In vitro protein production for structure determination with the RTS system by Cho (2002)
10.1016/S0014-5793(02)03048-X / FEBS Lett. / NMR analysis of in vitro-synthesized proteins without purification: a high-throughput approach by Guignard (2003)
{'key': '10.1016/j.tibtech.2004.08.012_bib68_1', 'series-title': 'Cell-Free Protein Expression', 'article-title': 'Isotope labeling of proteins for NMR spectroscopy using cell-free methods', 'author': 'Pelton', 'year': '2003'} / Cell-Free Protein Expression / Isotope labeling of proteins for NMR spectroscopy using cell-free methods by Pelton (2003)
{'key': '10.1016/j.tibtech.2004.08.012_bib69_1', 'series-title': 'Cell-Free Protein Expression', 'article-title': 'Production of a specifically labeled protein in mg quantities for NMR analysis', 'author': 'Fernholz', 'year': '2003'} / Cell-Free Protein Expression / Production of a specifically labeled protein in mg quantities for NMR analysis by Fernholz (2003)
10.1110/ps.0241203 / Protein Sci. / A wheat germ cell-free system is a novel way to screen protein folding and function by Morita (2003)
10.1023/A:1013203532303 / J. Struct. Funct. Genomics / Selenomethionine incorporation into a protein by cell-free synthesis by Kigawa (2002)
{'key': '10.1016/j.tibtech.2004.08.012_bib72', 'first-page': '1213', 'article-title': 'Structure of a conserved CoA-binding protein synthesized by a cell-free system', 'volume': 'D59', 'author': 'Wada', 'year': '2003', 'journal-title': 'Acta Crystallogr.'} / Acta Crystallogr. / Structure of a conserved CoA-binding protein synthesized by a cell-free system by Wada (2003)
10.1006/bbrc.2000.2556 / Biochem. Biophys. Res. Commun. / Sense codon-dependent introduction of unnatural amino acids into multiple sites of a protein by Kanda (2000)
10.1073/pnas.142220099 / Proc. Natl. Acad. Sci. U. S. A. / An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system by Kiga (2002)
{'key': '10.1016/j.tibtech.2004.08.012_bib75', 'series-title': 'Cell-Free Protein Expression', 'first-page': '81', 'article-title': 'Incorporation of fluorescent labels into cell-free produced proteins', 'author': 'Sengupta', 'year': '2003'} / Cell-Free Protein Expression / Incorporation of fluorescent labels into cell-free produced proteins by Sengupta (2003)
{'key': '10.1016/j.tibtech.2004.08.012_bib76', 'series-title': 'Cell-Free Protein Expression', 'first-page': '89', 'article-title': 'Expression of “tailor-made” proteins via incorporation of synthetic amino acids by using cell-free protein synthesis', 'author': 'Budisa', 'year': '2003'} / Cell-Free Protein Expression / Expression of “tailor-made” proteins via incorporation of synthetic amino acids by using cell-free protein synthesis by Budisa (2003)

Dates

Type	When
Created	20 years, 11 months ago (Sept. 10, 2004, 9:21 a.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 4:51 a.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 9:21 p.m.)
Issued	20 years, 11 months ago (Oct. 1, 2004)
Published	20 years, 11 months ago (Oct. 1, 2004)
Published Print	20 years, 11 months ago (Oct. 1, 2004)

Funders 0

None

BibTeX

@article{Spirin_2004, title={High-throughput cell-free systems for synthesis of functionally active proteins}, volume={22}, ISSN={0167-7799}, url={http://dx.doi.org/10.1016/j.tibtech.2004.08.012}, DOI={10.1016/j.tibtech.2004.08.012}, number={10}, journal={Trends in Biotechnology}, publisher={Elsevier BV}, author={Spirin, Alexander S.}, year={2004}, month=oct, pages={538–545} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        12
      ]
    ],
    "date-time": "2025-07-12T01:21:53Z",
    "timestamp": 1752283313305
  },
  "reference-count": 76,
  "publisher": "Elsevier BV",
  "issue": "10",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2004,
            10,
            1
          ]
        ],
        "date-time": "2004-10-01T00:00:00Z",
        "timestamp": 1096588800000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2004,
        10
      ]
    ]
  },
  "DOI": "10.1016/j.tibtech.2004.08.012",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        9,
        10
      ]
    ],
    "date-time": "2004-09-10T13:21:56Z",
    "timestamp": 1094822516000
  },
  "page": "538-545",
  "source": "Crossref",
  "is-referenced-by-count": 176,
  "title": "High-throughput cell-free systems for synthesis of functionally active proteins",
  "prefix": "10.1016",
  "volume": "22",
  "author": [
    {
      "given": "Alexander S.",
      "family": "Spirin",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib1",
      "series-title": "Cell-Free Translation Systems",
      "first-page": "3",
      "article-title": "Cell-free protein synthesis",
      "author": "Spirin",
      "year": "2002"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "1588",
      "DOI": "10.1073/pnas.47.10.1588",
      "article-title": "The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polynucleotides",
      "volume": "47",
      "author": "Nirenberg",
      "year": "1961",
      "journal-title": "Proc. Natl. Acad. Sci. U. S. A."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "1010",
      "DOI": "10.1073/pnas.57.4.1010",
      "article-title": "DNA-directed peptide synthesis II. The synthesis of the \u03b1-fragment of the enzyme \u03b2-galactosidase",
      "volume": "57",
      "author": "DeVries",
      "year": "1967",
      "journal-title": "Proc. Natl. Acad. Sci. U. S. A."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "751",
      "DOI": "10.1038/90802",
      "article-title": "Cell-free translation reconstituted from purified components",
      "volume": "19",
      "author": "Shimizu",
      "year": "2001",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "1162",
      "DOI": "10.1126/science.3055301",
      "article-title": "A continuous cell-free translation system capable of producing polypeptides in high yield",
      "volume": "242",
      "author": "Spirin",
      "year": "1988",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "463",
      "DOI": "10.1016/0378-1119(89)90521-0",
      "article-title": "Gene expression in a cell-free system on the preparative scale",
      "volume": "84",
      "author": "Baranov",
      "year": "1989",
      "journal-title": "Gene"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "4412",
      "DOI": "10.1093/nar/17.11.4412",
      "article-title": "Preparative synthesis of globin in a continuous cell-free translation system from rabbit reticulocytes",
      "volume": "17",
      "author": "Ryabova",
      "year": "1989",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib8",
      "series-title": "Frontiers in Bioprocessing II",
      "first-page": "31",
      "article-title": "Cell-free protein synthesis bioreactor",
      "author": "Spirin",
      "year": "1992"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "123",
      "DOI": "10.1016/0076-6879(93)17059-E",
      "article-title": "Gene expression in cell-free systems on preparative scale",
      "volume": "217",
      "author": "Baranov",
      "year": "1993",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "166",
      "DOI": "10.1093/oxfordjournals.jbchem.a123551",
      "article-title": "A continuous cell-free protein synthesis system for coupled transcription-translation",
      "volume": "110",
      "author": "Kigawa",
      "year": "1991",
      "journal-title": "J. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "221",
      "DOI": "10.1016/0168-1656(92)90157-5",
      "article-title": "Production of an enzymatic active protein using a continuous flow cell-free system",
      "volume": "25",
      "author": "Endo",
      "year": "1992",
      "journal-title": "J. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "389",
      "DOI": "10.1016/0003-2697(92)90383-I",
      "article-title": "High efficiency cell-free synthesis of proteins: refinement of the coupled transcription/translation system",
      "volume": "206",
      "author": "Kudlicki",
      "year": "1992",
      "journal-title": "Anal. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "125",
      "DOI": "10.1111/j.1470-8744.1992.tb00217.x",
      "article-title": "Preparative in vitro synthesis of bioactive human interleukin-2 in a continuous flow translation system",
      "volume": "16",
      "author": "Kolosov",
      "year": "1992",
      "journal-title": "Biotechnol. Appl. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "289",
      "DOI": "10.1006/abio.1993.1490",
      "article-title": "Synthesis of preparative amounts of biologically active interleukin-6 using a continuous-flow cell-free translation system",
      "volume": "214",
      "author": "Volyanik",
      "year": "1993",
      "journal-title": "Anal. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib15",
      "series-title": "Cell-Free Translation Systems",
      "first-page": "91",
      "article-title": "Continuous-flow and continuous-exchange cell-free translation systems and reactors",
      "author": "Shirokov",
      "year": "2002"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "645",
      "DOI": "10.1021/bp960052l",
      "article-title": "A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane",
      "volume": "12",
      "author": "Kim",
      "year": "1996",
      "journal-title": "Biotechnol. Prog."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "15",
      "DOI": "10.1016/S0014-5793(98)01620-2",
      "article-title": "Cell-free production and stable-isotope labelling of milligram quantities of proteins",
      "volume": "442",
      "author": "Kigawa",
      "year": "1999",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "559",
      "DOI": "10.1073/pnas.97.2.559",
      "article-title": "A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomes",
      "volume": "97",
      "author": "Madin",
      "year": "2000",
      "journal-title": "Proc. Natl. Acad. Sci. U. S. A."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib19",
      "doi-asserted-by": "crossref",
      "first-page": "14652",
      "DOI": "10.1073/pnas.232580399",
      "article-title": "A cell-free protein synthesis system for high-throughput proteomics",
      "volume": "99",
      "author": "Sawasaki",
      "year": "2002",
      "journal-title": "Proc. Natl. Acad. Sci. U. S. A."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib20",
      "doi-asserted-by": "crossref",
      "first-page": "45",
      "DOI": "10.1023/B:JSFG.0000029208.83739.49",
      "article-title": "High-throughput, genome scale protein production method based on the wheat germ cell-free expression system",
      "volume": "5",
      "author": "Endo",
      "year": "2004",
      "journal-title": "J. Struct. Funct. Genomics"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "948",
      "DOI": "10.2144/01314pf01",
      "article-title": "High-yield, in vitro protein expression using a continuous-exchange, coupled transcription/translation system",
      "volume": "31",
      "author": "Martin",
      "year": "2001",
      "journal-title": "Biotechniques"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "73",
      "DOI": "10.2174/1389203033380359",
      "article-title": "Rapid Translation System (RTS): A promising alternative for recombinant protein production",
      "volume": "4",
      "author": "Betton",
      "year": "2003",
      "journal-title": "Curr. Protein Pept. Sci."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "194",
      "DOI": "10.1002/(SICI)1097-0290(19990720)64:2<194::AID-BIT8>3.0.CO;2-5",
      "article-title": "Efficient coupled transcription/translation for PCR template by a hollow fiber membrane bioreactor",
      "volume": "64",
      "author": "Nakano",
      "year": "1999",
      "journal-title": "Biotechnol. Bioeng."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib24",
      "series-title": "Cell-Free Translation Systems",
      "first-page": "121",
      "article-title": "Matrix reactor: A new scalable principle for cell-free protein expression",
      "author": "Buchberger",
      "year": "2002"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "102",
      "DOI": "10.1016/S0014-5793(02)02329-3",
      "article-title": "A bilayer cell-free protein synthesis system for high-throughout screening of gene products",
      "volume": "514",
      "author": "Sawasaki",
      "year": "2002",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "466",
      "DOI": "10.1074/mcp.T200004-MCP200",
      "article-title": "An improved protein bioreactor. Efficient product isolation during in vitro protein biosynthesis via affinity tag",
      "volume": "6",
      "author": "Lamla",
      "year": "2002",
      "journal-title": "Mol. Cell. Proteomics"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "881",
      "DOI": "10.1111/j.1432-1033.1996.0881u.x",
      "article-title": "A highly efficient cell-free protein synthesis system from Escherichia coli",
      "volume": "239",
      "author": "Kim",
      "year": "1996",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "385",
      "DOI": "10.1021/bp000031y",
      "article-title": "Prolonging cell-free protein synthesis by selective reagent additions",
      "volume": "16",
      "author": "Kim",
      "year": "2000",
      "journal-title": "Biotechnol. Prog."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib29",
      "doi-asserted-by": "crossref",
      "unstructured": "Michel-Reydellet, N. et al. Amino acid stabilization for cell-free protein synthesis by modification of the E. coli genome. Metab. Eng. (in press)",
      "DOI": "10.1016/j.ymben.2004.01.003"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "184",
      "DOI": "10.1006/abio.1995.1208",
      "article-title": "Acetyl phosphate as an energy source for bacterial cell-free translation systems",
      "volume": "226",
      "author": "Ryabova",
      "year": "1995",
      "journal-title": "Anal. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "180",
      "DOI": "10.1002/(SICI)1097-0290(1999)66:3<180::AID-BIT6>3.0.CO;2-S",
      "article-title": "Prolonging cell-free protein synthesis with a novel ATP regeneration system",
      "volume": "66",
      "author": "Kim",
      "year": "1999",
      "journal-title": "Biotechnol. Bioeng."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib32",
      "doi-asserted-by": "crossref",
      "first-page": "309",
      "DOI": "10.1002/bit.1121",
      "article-title": "Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis",
      "volume": "74",
      "author": "Kim",
      "year": "2001",
      "journal-title": "Biotechnol. Bioeng."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "19",
      "DOI": "10.1002/bit.20026",
      "article-title": "Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis",
      "volume": "86",
      "author": "Jewett",
      "year": "2004",
      "journal-title": "Biotechnol. Bioeng."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "102",
      "DOI": "10.1021/bp0341693",
      "article-title": "Rapid expression and purification of 100nmol quantities of active protein using cell-free protein synthesis",
      "volume": "20",
      "author": "Jewett",
      "year": "2004",
      "journal-title": "Biotechnol. Prog."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "247",
      "DOI": "10.1111/j.1432-1033.1976.tb10656.x",
      "article-title": "An efficient mRNA-dependent translation system from reticulocyte lysates",
      "volume": "67",
      "author": "Pelham",
      "year": "1976",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib36",
      "doi-asserted-by": "crossref",
      "first-page": "3631",
      "DOI": "10.1002/j.1460-2075.1994.tb06670.x",
      "article-title": "Folding of firefly luciferase during translation in a cell-free system",
      "volume": "13",
      "author": "Kolb",
      "year": "1994",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib37",
      "doi-asserted-by": "crossref",
      "first-page": "16597",
      "DOI": "10.1074/jbc.M002030200",
      "article-title": "Co-translational folding of an eukaryotic multidomain protein in a prokaryotic cell-free system",
      "volume": "275",
      "author": "Kolb",
      "year": "2000",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib38",
      "doi-asserted-by": "crossref",
      "first-page": "79",
      "DOI": "10.1038/nbt0197-79",
      "article-title": "Functional antibody production using cell-free translation: Effect of protein disulfide isomerase and chaperones",
      "volume": "15",
      "author": "Ryabova",
      "year": "1997",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib39",
      "doi-asserted-by": "crossref",
      "first-page": "290",
      "DOI": "10.1016/S0014-5793(02)02383-9",
      "article-title": "Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation system",
      "volume": "514",
      "author": "Jiang",
      "year": "2002",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib40",
      "doi-asserted-by": "crossref",
      "first-page": "423",
      "DOI": "10.1093/protein/gzg054",
      "article-title": "Improvement of H2O2 stability of manganese peroxidase by combinatorial mutagenesis and high throughput screening using in vitro expression with protein disulfide isomerase",
      "volume": "16",
      "author": "Miyasaki-Imamura",
      "year": "2003",
      "journal-title": "Protein Eng."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib41",
      "doi-asserted-by": "crossref",
      "first-page": "188",
      "DOI": "10.1002/bit.10827",
      "article-title": "Enhancing multiple disulfide bonded protein folding in a cell-free system",
      "volume": "86",
      "author": "Yin",
      "year": "2004",
      "journal-title": "Biotechnol. Bioeng."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib42",
      "doi-asserted-by": "crossref",
      "first-page": "66",
      "DOI": "10.1016/S0014-5793(04)00025-0",
      "article-title": "Cell-free production of active E. coli thioredoxin reductase and glutathione reductase",
      "volume": "559",
      "author": "Knapp",
      "year": "2004",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib43",
      "doi-asserted-by": "crossref",
      "first-page": "10646",
      "DOI": "10.1074/jbc.272.16.10646",
      "article-title": "Cotranslational folding of globin",
      "volume": "272",
      "author": "Komar",
      "year": "1997",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib44_1",
      "series-title": "Cell-Free Protein Expression",
      "article-title": "Optimization of cell-free expression of FAD-dependent D-amino acid oxidase",
      "author": "Lehmann",
      "year": "2003"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib45",
      "doi-asserted-by": "crossref",
      "first-page": "122",
      "DOI": "10.1002/bit.10865",
      "article-title": "Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts of Escherichia coli",
      "volume": "85",
      "author": "Kim",
      "year": "2004",
      "journal-title": "Biotechnol. Bioeng."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib46",
      "doi-asserted-by": "crossref",
      "unstructured": "Yang, J. et al. Expression of active murine granulocyte-macrophage colony-stimulating factor in an Escherichia coli cell-free system. Biotechnol. Prog. (in press)",
      "DOI": "10.1021/bp034350b"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib47",
      "series-title": "Cell-Free Translation Systems",
      "first-page": "149",
      "article-title": "Expression of soluble murine endostatin with the RTS system",
      "author": "Traviglia",
      "year": "2000"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib48",
      "series-title": "Cell-Free Translation Systems",
      "first-page": "157",
      "article-title": "Cell-free expression of soluble human erythropoietin",
      "author": "Nemetz",
      "year": "2000"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib49",
      "series-title": "Cell-Free Translation Systems",
      "first-page": "165",
      "article-title": "Cell-free expression of a 127kDa protein: the catalytic subunit of human telomerase",
      "author": "Steigerwald",
      "year": "2000"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib50",
      "series-title": "Cell-Free Translation Systems",
      "first-page": "175",
      "article-title": "The expression of disulfide bonded proteins in cell-free protein expression",
      "author": "Fernholz",
      "year": "2000"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib51",
      "series-title": "Cell-Free Protein Expression",
      "first-page": "125",
      "article-title": "Cell-free expression of proteins containing multiple disulfide bonds",
      "author": "Kim",
      "year": "2003"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib52_1",
      "series-title": "Cell-Free Protein Expression",
      "article-title": "Cell-free synthesis of membrane proteins on a preparative scale",
      "author": "Maurer",
      "year": "2003"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib53",
      "series-title": "Cell-Free Protein Expression",
      "first-page": "109",
      "article-title": "Screening and increasing soluble expression of recombinant proteins using the RTS 100 E. coli HY in a 96-well format",
      "author": "Busso",
      "year": "2003"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib54",
      "doi-asserted-by": "crossref",
      "first-page": "568",
      "DOI": "10.1111/j.1432-1033.2003.03959.x",
      "article-title": "High level cell-free expression and specific labeling of integral membrane proteins",
      "volume": "271",
      "author": "Klammt",
      "year": "2004",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib55",
      "doi-asserted-by": "crossref",
      "first-page": "2632",
      "DOI": "10.1016/S0021-9258(18)52291-2",
      "article-title": "Use of in vitro synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies",
      "volume": "262",
      "author": "Lesley",
      "year": "1991",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib56",
      "doi-asserted-by": "crossref",
      "first-page": "268",
      "DOI": "10.1016/S0014-5793(97)01011-9",
      "article-title": "Direct expression of PCR products in a cell-free transcription/translation system: Synthesis of antibacterial peptide cecropin",
      "volume": "414",
      "author": "Martemyanov",
      "year": "1997",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib57",
      "doi-asserted-by": "crossref",
      "first-page": "4339",
      "DOI": "10.1093/nar/26.19.4339",
      "article-title": "In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation",
      "volume": "26",
      "author": "Ohuchi",
      "year": "1998",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib58",
      "doi-asserted-by": "crossref",
      "first-page": "395",
      "DOI": "10.1016/S0022-2836(02)00094-3",
      "article-title": "High-throughput, cloning independent protein library construction by combining single-molecule DNA amplification with in vitro expression",
      "volume": "318",
      "author": "Rungpragayphan",
      "year": "2002",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib59",
      "doi-asserted-by": "crossref",
      "first-page": "147",
      "DOI": "10.1016/S0014-5793(03)00251-5",
      "article-title": "PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries",
      "volume": "540",
      "author": "Rungpragayphan",
      "year": "2003",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib60",
      "doi-asserted-by": "crossref",
      "first-page": "39",
      "DOI": "10.1016/S1367-5931(02)00019-4",
      "article-title": "Protein expression systems for structural genomics and proteomics",
      "volume": "7",
      "author": "Yokoyama",
      "year": "2003",
      "journal-title": "Curr. Opin. Chem. Biol."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib61",
      "doi-asserted-by": "crossref",
      "first-page": "233",
      "DOI": "10.1016/S0165-022X(03)00049-6",
      "article-title": "Expression of soluble recombinant proteins in a cell-free system using a 96-well format",
      "volume": "55",
      "author": "Busso",
      "year": "2003",
      "journal-title": "J. Biochem. Biophys. Methods"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib62",
      "series-title": "Cell-Free Protein Expression",
      "first-page": "15",
      "article-title": "Expression-PCR: from gene pools to purified proteins within 1 day",
      "author": "Merk",
      "year": "2003"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib63",
      "doi-asserted-by": "crossref",
      "first-page": "147",
      "DOI": "10.1093/protein/15.2.147",
      "article-title": "Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate-binding site",
      "volume": "15",
      "author": "Yang",
      "year": "2002",
      "journal-title": "Protein Eng."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib64",
      "doi-asserted-by": "crossref",
      "first-page": "585",
      "DOI": "10.1016/S0022-2836(03)00782-4",
      "article-title": "Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high throughput screening using single-molecule PCR and in vitro expression",
      "volume": "331",
      "author": "Koga",
      "year": "2003",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib65",
      "series-title": "Cell-Free Translation Systems",
      "first-page": "219",
      "article-title": "Selective labeling of proteins in the RTS 500 system",
      "author": "Betton",
      "year": "2002"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib66",
      "series-title": "Cell-Free Translation Systems",
      "first-page": "227",
      "article-title": "In vitro protein production for structure determination with the RTS system",
      "author": "Cho",
      "year": "2002"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib67",
      "doi-asserted-by": "crossref",
      "first-page": "159",
      "DOI": "10.1016/S0014-5793(02)03048-X",
      "article-title": "NMR analysis of in vitro-synthesized proteins without purification: a high-throughput approach",
      "volume": "524",
      "author": "Guignard",
      "year": "2003",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib68_1",
      "series-title": "Cell-Free Protein Expression",
      "article-title": "Isotope labeling of proteins for NMR spectroscopy using cell-free methods",
      "author": "Pelton",
      "year": "2003"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib69_1",
      "series-title": "Cell-Free Protein Expression",
      "article-title": "Production of a specifically labeled protein in mg quantities for NMR analysis",
      "author": "Fernholz",
      "year": "2003"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib70",
      "doi-asserted-by": "crossref",
      "first-page": "1216",
      "DOI": "10.1110/ps.0241203",
      "article-title": "A wheat germ cell-free system is a novel way to screen protein folding and function",
      "volume": "12",
      "author": "Morita",
      "year": "2003",
      "journal-title": "Protein Sci."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib71",
      "doi-asserted-by": "crossref",
      "first-page": "29",
      "DOI": "10.1023/A:1013203532303",
      "article-title": "Selenomethionine incorporation into a protein by cell-free synthesis",
      "volume": "2",
      "author": "Kigawa",
      "year": "2002",
      "journal-title": "J. Struct. Funct. Genomics"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib72",
      "first-page": "1213",
      "article-title": "Structure of a conserved CoA-binding protein synthesized by a cell-free system",
      "volume": "D59",
      "author": "Wada",
      "year": "2003",
      "journal-title": "Acta Crystallogr."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib73",
      "doi-asserted-by": "crossref",
      "first-page": "1136",
      "DOI": "10.1006/bbrc.2000.2556",
      "article-title": "Sense codon-dependent introduction of unnatural amino acids into multiple sites of a protein",
      "volume": "270",
      "author": "Kanda",
      "year": "2000",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib74",
      "doi-asserted-by": "crossref",
      "first-page": "9715",
      "DOI": "10.1073/pnas.142220099",
      "article-title": "An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system",
      "volume": "99",
      "author": "Kiga",
      "year": "2002",
      "journal-title": "Proc. Natl. Acad. Sci. U. S. A."
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib75",
      "series-title": "Cell-Free Protein Expression",
      "first-page": "81",
      "article-title": "Incorporation of fluorescent labels into cell-free produced proteins",
      "author": "Sengupta",
      "year": "2003"
    },
    {
      "key": "10.1016/j.tibtech.2004.08.012_bib76",
      "series-title": "Cell-Free Protein Expression",
      "first-page": "89",
      "article-title": "Expression of \u201ctailor-made\u201d proteins via incorporation of synthetic amino acids by using cell-free protein synthesis",
      "author": "Budisa",
      "year": "2003"
    }
  ],
  "container-title": "Trends in Biotechnology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0167779904002343?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0167779904002343?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        14
      ]
    ],
    "date-time": "2024-01-14T09:51:16Z",
    "timestamp": 1705225876000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S0167779904002343"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2004,
        10
      ]
    ]
  },
  "references-count": 76,
  "journal-issue": {
    "issue": "10",
    "published-print": {
      "date-parts": [
        [
          2004,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "S0167779904002343"
  ],
  "URL": "http://dx.doi.org/10.1016/j.tibtech.2004.08.012",
  "relation": {},
  "ISSN": [
    "0167-7799"
  ],
  "subject": [],
  "container-title-short": "Trends in Biotechnology",
  "published": {
    "date-parts": [
      [
        2004,
        10
      ]
    ]
  }
}