DOI: 10.1016/j.tibtech.2003.10.002. Molecular farming in plants: host systems and expression technology

Molecular farming in plants: host systems and expression technology

10.1016/j.tibtech.2003.10.002

Crossref journal-article

Elsevier BV

Trends in Biotechnology (78)

Bibliography

Twyman, R. M., Stoger, E., Schillberg, S., Christou, P., & Fischer, R. (2003). Molecular farming in plants: host systems and expression technology. Trends in Biotechnology, 21(12), 570â578.

Authors 5

Richard M. Twyman (first)
Eva Stoger (additional)
Stefan Schillberg (additional)
Paul Christou (additional)
Rainer Fischer (additional)

References 86 Referenced 544

10.1023/A:1009676322162 / Mol. Breed. / Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification by Hood (1997)
10.1023/A:1009622429758 / Mol. Breed. / Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plants by Witcher (1998)
10.1002/(SICI)1097-0290(19981005)60:1<44::AID-BIT5>3.0.CO;2-0 / Biotechnol. Bioeng. / Processing of transgenic corn seed and its effect on the recovery of recombinant β-glucuronidase by Kusnadi (1998)
10.1021/bp980047c / Biotechnol. Prog. / Process and economic evaluation of the extraction and purification of recombinant β-glucuronidase from transgenic corn by Evangelista (1998)
10.1023/A:1008975123362 / Transgenic Res. / Molecular farming of pharmaceutical proteins by Fischer (2000)
10.1016/S0958-1669(00)00244-5 / Curr. Opin. Biotechnol. / Transgenic plants as protein factories by Giddings (2001)
Hood, E.E. Plants as enzyme factories. In Handbook of Plant Biotechnology (Christou, P. and Klee, H., eds), Wiley-VCH (in press). (10.1002/0470869143.kc042)
10.1016/S0958-1669(02)00351-8 / Curr. Opin. Biotechnol. / Monoclonal antibody manufacturing in transgenic plants – myths and realities by Hood (2002)
10.1023/A:1015880218651 / Phytochem. Rev. / Antibody molecular farming in plants and plant cells by Schillberg (2002)
10.1016/S1471-4914(02)02360-2 / Trends Mol. Med. / Edible plant vaccines: applications for prophylactic and therapeutic molecular medicine by Mason (2002)
10.1093/ps/77.6.878 / Poult. Sci. / Soybeans transformed with fungal phytase gene improve phosphorus availability for broilers by Denbow (1998)
10.1023/A:1009653011948 / Mol. Breed. / Improved plant-based production of E1 endoglucanase using potato: expression optimization and tissue targeting by Dai (2000)
10.1023/A:1009667524690 / Mol. Breed. / Accumulation of a thermostable endo-1,4-β-D-glucanase in the apoplast of Arabidopsis thaliana leaves by Ziegler (2000)
10.1023/A:1013338312948 / Mol. Breed. / Dramatic effects of truncation and sub-cellular targeting on the accumulation of recombinant microbial cellulase in tobacco by Ziegelhoffer (2001)
10.1023/A:1009657701588 / Mol. Breed. / Transgenic pea seeds as bioreactors for the production of a single-chain Fv fragment (scFV) antibody used in cancer diagnosis and therapy by Perrin (2000)
10.1023/A:1006301519427 / Plant Mol. Biol. / Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies by Stoger (2000)
Moloney, M. et al. (2003) Oil bodies and associated proteins as affinity matrices. US Patent 6509453.
10.1023/A:1009697604758 / Mol. Breed. / Plasma membrane display of anti-viral single chain Fv fragments confers resistance to tobacco mosaic virus by Schillberg (2000)
10.1038/8643 / Nat. Biotechnol. / Production of recombinant proteins in plant root exudates by Borisjuk (1999)
10.1104/pp.124.3.927 / Plant Physiol. / Production of recombinant proteins in tobacco guttation fluid by Komarnytsky (2000)
10.1023/A:1023909331482 / Plant Mol. Biol. / Rhizosecretion of a monoclonal antibody protein complex from transgenic tobacco roots by Drake (2003)
10.1111/j.1470-8744.1999.tb00900.x / Biotechnol. Appl. Biochem. / Towards molecular farming in the future: transient protein expression in plants by Fischer (1999)
10.1111/j.1470-8744.1999.tb00899.x / Biotechnol. Appl. Biochem. / Towards molecular farming in the future: using plant-cell-suspension cultures as bioreactors by Fischer (1999)
{'key': '10.1016/j.tibtech.2003.10.002_BIB24', 'first-page': '110', 'article-title': 'The biosafety of molecular farming in plants', 'volume': '5', 'author': 'Commandeur', 'year': '2003', 'journal-title': 'AgBiotechNet'} / AgBiotechNet / The biosafety of molecular farming in plants by Commandeur (2003)
10.1016/S0958-1669(02)00303-8 / Curr. Opin. Biotechnol. / Plantibodies: applications, advantages and bottlenecks by Stoger (2002)
10.1126/science.7732380 / Science / Generation and assembly of secretory antibodies in plants by Ma (1995)
10.1016/S1389-0344(01)00102-2 / Biomol. Eng. / Production of secretory IgA antibodies in plants by Larrick (2001)
{'key': '10.1016/j.tibtech.2003.10.002_BIB28', 'series-title': 'Carbohydrates in Chemistry and Biology', 'first-page': '1043', 'article-title': "Metabolic engineering glycosylation: biotechnology's challenge to the glycobiologist in the new millennium", 'author': 'Warner', 'year': '2000'} / Carbohydrates in Chemistry and Biology / Metabolic engineering glycosylation: biotechnology's challenge to the glycobiologist in the new millennium by Warner (2000)
10.1021/ja017881+ / J. Am. Chem. Soc. / Efficient chemoenzymatic synthesis of O-linked sialyl oligosaccharides by Blixt (2002)
10.1073/pnas.031419998 / Proc. Natl. Acad. Sci. U. S. A. / Galactose-extended glycans of antibodies produced by transgenic plants by Bakker (2001)
10.1023/A:1008976219939 / Transgenic Res. / A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice by Chargelegue (2000)
{'key': '10.1016/j.tibtech.2003.10.002_BIB32', 'first-page': '161', 'article-title': 'Plant genes and their expression', 'volume': '80', 'author': 'Tyagi', 'year': '2001', 'journal-title': 'Curr. Sci.'} / Curr. Sci. / Plant genes and their expression by Tyagi (2001)
10.1007/BF01969712 / Transgenic Res. / Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants by Christensen (1996)
10.1007/BF00232980 / Plant Cell Rep. / Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.) by Vain (1996)
10.1016/S0958-1669(00)00073-2 / Curr. Opin. Biotechnol. / Chemical-inducible systems for regulated expression of plant genes by Zuo (2000)
{'key': '10.1016/j.tibtech.2003.10.002_BIB36', 'first-page': '95', 'article-title': 'Transgenic plants for therapeutic proteins: linking upstream and downstream technologies', 'volume': '240', 'author': 'Cramer', 'year': '1999', 'journal-title': 'Curr. Top. Microbiol. Immunol.'} / Curr. Top. Microbiol. Immunol. / Transgenic plants for therapeutic proteins: linking upstream and downstream technologies by Cramer (1999)
10.1016/S1360-1385(99)01484-3 / Trends Plant Sci. / Current perspectives on mRNA stability in plants: multiple levels and mechanisms of control by Gutiérrez (1999)
10.1016/S1369-5266(02)00290-X / Curr. Opin. Plant Biol. / Regulation of translational initiation in plants by Kawaguchi (2002)
10.1007/BF00039392 / Plant Mol. Biol. / Optimizing expression of transgenes with an emphasis on post-transcriptional events by Koziel (1996)
10.1016/S0959-437X(00)00128-3 / Curr. Opin. Genet. Dev. / The silence of the genes by Plasterk (2000)
{'key': '10.1016/j.tibtech.2003.10.002_BIB41', 'first-page': '107', 'article-title': 'Foreign DNA: integration and expression in transgenic plants', 'volume': 'Vol. 24', 'author': 'Twyman', 'year': '2002'} / Foreign DNA: integration and expression in transgenic plants by Twyman (2002)
10.1016/S1369-5266(02)00291-1 / Curr. Opin. Plant Biol. / RNA silencing: small RNAs as ubiquitous regulators of gene expression by Voinnet (2002)
10.1023/A:1023941407376 / Plant Mol. Biol. / Transgene integration, organization and interaction in plants by Kohli (2003)
10.1023/A:1008993730505 / Transgenic Res. / Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns by Fu (2000)
10.1023/A:1009638600492 / Mol. Breed. / Intracellular expression of TMV-specific single-chain Fv fragments leads to improved virus resistance in Nicotiana tabacum by Zimmermann (1998)
10.1023/A:1008937011213 / Transgenic Res. / Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum by Schillberg (1999)
10.1046/j.1432-1327.1999.00060.x / Eur. J. Biochem. / High-level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida by De Jaeger (1999)
10.1074/jbc.M201245200 / J. Biol. Chem. / Formation of disulfide bridges by a single-chain Fv antibody in the reducing ectopic environment of the plant cytosol by Schouten (2002)
10.1046/j.1432-1033.2002.03302.x / Eur. J. Biochem. / ER-resident chaperone interactions with recombinant antibodies in transgenic plants by Nuttall (2002)
10.1023/A:1006029617949 / Plant Mol. Biol. / Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity by Conrad (1998)
Sriraman, R. et al. Glycosylation of recombinant antibodies in plants. Proceedings of the Ranbaxy Science Foundation Symposium 2002, Ranbaxy Science Foundation, New Delhi (in press).
10.1016/S1360-1385(01)02193-8 / Trends Plant Sci. / Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology by Daniell (2002)
10.1038/73796 / Nat. Biotechnol. / High-yield production of a human therapeutic protein in tobacco chloroplasts by Staub (2000)
{'key': '10.1016/j.tibtech.2003.10.002_BIB54', 'first-page': '77', 'article-title': 'A chloroplast transgenic approach to hyper-express and purify human serum albumin, a protein highly susceptible to proteolytic degradation', 'volume': '1', 'author': 'Millan', 'year': '2003', 'journal-title': 'Plant Biotechnol.'} / Plant Biotechnol. / A chloroplast transgenic approach to hyper-express and purify human serum albumin, a protein highly susceptible to proteolytic degradation by Millan (2003)
10.1006/jmbi.2001.4921 / J. Mol. Biol. / Expression of the native cholera B toxin subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts by Daniell (2001)
10.1093/nar/gkg221 / Nucleic Acids Res. / Expression of tetanus toxin fragment C in tobacco chloroplasts by Tregoning (2003)
10.1023/A:1022168321380 / Mol. Breed. / Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme by Leelavathi (2003)
10.1128/AEM.68.7.3345-3351.2002 / Appl. Environ. Microbiol. / In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria by Kay (2002)
10.1038/nbt772 / Nat. Biotechnol. / Immunomodulation of enzyme function in plants by single domain antibody fragments by Jobling (2003)
10.1023/A:1019714614827 / Mol. Breed. / Practical considerations for pharmaceutical antibody production in different crop systems by Stöger (2002)
D'Aoust, M.A. Perennial plants as a production system for pharmaceuticals. In Handbook of Plant Biotechnology (Christou, P. and Klee, H., eds), Wiley-VCH (in press). (10.1002/0470869143.kc043)
10.1038/4344 / Nat. Biotechnol. / A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes by Zeitlin (1998)
10.1002/(SICI)1097-0290(19990720)64:2<135::AID-BIT2>3.0.CO;2-Q / Biotechnol. Bioeng. / Production of a diagnostic monoclonal antibody in perennial alfalfa plants by Khoudi (1999)
10.1096/fasebj.13.13.1796 / FASEB J. / A plant-derived edible vaccine against hepatitis B virus by Kapusta (1999)
10.1016/S0141-0229(01)00502-6 / Enzyme & Microbial Technol / From green plants to industrial enzymes by Hood (2002)
{'key': '10.1016/j.tibtech.2003.10.002_BIB66', 'first-page': '485', 'article-title': 'Microplate assay for soybean seed coat peroxidase activity', 'volume': '24', 'author': 'Vierling', 'year': '1996', 'journal-title': 'Seed Science Technol.'} / Seed Science Technol. / Microplate assay for soybean seed coat peroxidase activity by Vierling (1996)
10.1038/nm0598-607 / Nat. Med. / Immunogenicity in humans of a recombinant bacterial-antigen delivered in transgenic potato by Tacket (1998)
10.1086/315653 / J. Infect. Dis. / Human immune responses to a novel Norwalk virus vaccine delivered in transgenic potatoes by Tacket (2000)
10.1038/81153 / Nat. Biotechnol. / Production of hepatitis B surface antigen in transgenic plants for oral immunization by Richter (2000)
10.1023/A:1022912130286 / Transgenic Res. / Expression of rotavirus capsid protein VP6 in transgenic potato and its oral immunogenicity in mice by Yu (2003)
10.1023/A:1009676832273 / Mol. Breed. / Potato tubers as a biofactory for recombinant antibodies by Artsaenko (1998)
10.1023/A:1020306917914 / Mol. Breed. / Expression of antibodies and Fab fragments in transgenic potato plants: a case study for bulk production in crop plants by De Wilde (2002)
10.1023/A:1026752805494 / Mol. Breed. / Biopharming the SimpliRED™ HIV diagnostic reagent in barley, potato and tobacco by Schunmann (2002)
10.1023/A:1008977630179 / Transgenic Res. / Expression of full-length bioactive antimicrobial human lactoferrin in potato plants by Chong (2000)
10.1023/A:1018410712288 / Transgenic Res. / Expression of the human milk protein β-casein in transgenic potato plants by Chong (1997)
10.1038/nbt1295-1484 / Biotechnology (N.Y.) / Expression of the rabies virus glycoprotein in transgenic tomatoes by McGarvey (1995)
10.1016/S0264-410X(02)00603-5 / Vaccine / Vaccine antigen production in transgenic plants: strategies, gene constructs and perspectives by Sala (2003)
10.1016/S0168-9452(96)04541-4 / Plant Sci. / An agrobacterium-mediated transient gene expression system for intact leaves by Kapila (1997)
10.1096/fj.01-0363fje / FASEB J. / A carcinoembryonic antigen-specific diabody produced in tobacco by Vaquero (2002)
10.1016/S0022-1759(98)00149-5 / J. Immunol. Methods / Expression and assembly of a full-length monoclonal antibody in plants using a plant virus vector by Verch (1998)
10.1073/pnas.96.2.703 / Proc. Natl. Acad. Sci. U. S. A. / Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants by McCormick (1999)
Mor, T.S. and Mason, H.S. Plants as a source of subunit vaccines. In Handbook of Plant Biotechnology (Christou, P. and Klee, H., eds), Wiley-VCH (in press). (10.1002/0470869143.kc041)
10.1016/S0958-1669(00)00086-0 / Curr. Opin. Biotechnol. / Foreign protein production in plant tissue cultures by Doran (2000)
10.1023/A:1008969031219 / Transgenic Res. / Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies by Torres (1999)
10.1093/glycob/cwg024 / Glycobiology / Immunoreactivity in mammals of two typical plant glyco-epitopes, core α(1,3)-fucose and core xylose by Bardor (2003)
10.1023/A:1006012005654 / Plant Mol. Biol. / N-glycoprotein biosynthesis in plants: recent developments and future trends by Lerouge (1998)

Dates

Type	When
Created	21 years, 9 months ago (Nov. 6, 2003, 4:52 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 3:14 a.m.)
Indexed	5 days, 3 hours ago (Aug. 28, 2025, 8:18 a.m.)
Issued	21 years, 9 months ago (Dec. 1, 2003)
Published	21 years, 9 months ago (Dec. 1, 2003)
Published Print	21 years, 9 months ago (Dec. 1, 2003)

Funders 0

None

BibTeX

@article{Twyman_2003, title={Molecular farming in plants: host systems and expression technology}, volume={21}, ISSN={0167-7799}, url={http://dx.doi.org/10.1016/j.tibtech.2003.10.002}, DOI={10.1016/j.tibtech.2003.10.002}, number={12}, journal={Trends in Biotechnology}, publisher={Elsevier BV}, author={Twyman, Richard M. and Stoger, Eva and Schillberg, Stefan and Christou, Paul and Fischer, Rainer}, year={2003}, month=dec, pages={570–578} }

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      "author": "Stoger",
      "year": "2000",
      "journal-title": "Plant Mol. Biol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB17",
      "unstructured": "Moloney, M. et al. (2003) Oil bodies and associated proteins as affinity matrices. US Patent 6509453."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB18",
      "doi-asserted-by": "crossref",
      "first-page": "317",
      "DOI": "10.1023/A:1009697604758",
      "article-title": "Plasma membrane display of anti-viral single chain Fv fragments confers resistance to tobacco mosaic virus",
      "volume": "6",
      "author": "Schillberg",
      "year": "2000",
      "journal-title": "Mol. Breed."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB19",
      "doi-asserted-by": "crossref",
      "first-page": "466",
      "DOI": "10.1038/8643",
      "article-title": "Production of recombinant proteins in plant root exudates",
      "volume": "17",
      "author": "Borisjuk",
      "year": "1999",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB20",
      "doi-asserted-by": "crossref",
      "first-page": "927",
      "DOI": "10.1104/pp.124.3.927",
      "article-title": "Production of recombinant proteins in tobacco guttation fluid",
      "volume": "124",
      "author": "Komarnytsky",
      "year": "2000",
      "journal-title": "Plant Physiol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB21",
      "doi-asserted-by": "crossref",
      "first-page": "233",
      "DOI": "10.1023/A:1023909331482",
      "article-title": "Rhizosecretion of a monoclonal antibody protein complex from transgenic tobacco roots",
      "volume": "52",
      "author": "Drake",
      "year": "2003",
      "journal-title": "Plant Mol. Biol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "113",
      "DOI": "10.1111/j.1470-8744.1999.tb00900.x",
      "article-title": "Towards molecular farming in the future: transient protein expression in plants",
      "volume": "30",
      "author": "Fischer",
      "year": "1999",
      "journal-title": "Biotechnol. Appl. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB23",
      "doi-asserted-by": "crossref",
      "first-page": "109",
      "DOI": "10.1111/j.1470-8744.1999.tb00899.x",
      "article-title": "Towards molecular farming in the future: using plant-cell-suspension cultures as bioreactors",
      "volume": "30",
      "author": "Fischer",
      "year": "1999",
      "journal-title": "Biotechnol. Appl. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB24",
      "first-page": "110",
      "article-title": "The biosafety of molecular farming in plants",
      "volume": "5",
      "author": "Commandeur",
      "year": "2003",
      "journal-title": "AgBiotechNet"
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB25",
      "doi-asserted-by": "crossref",
      "first-page": "161",
      "DOI": "10.1016/S0958-1669(02)00303-8",
      "article-title": "Plantibodies: applications, advantages and bottlenecks",
      "volume": "13",
      "author": "Stoger",
      "year": "2002",
      "journal-title": "Curr. Opin. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "716",
      "DOI": "10.1126/science.7732380",
      "article-title": "Generation and assembly of secretory antibodies in plants",
      "volume": "268",
      "author": "Ma",
      "year": "1995",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "87",
      "DOI": "10.1016/S1389-0344(01)00102-2",
      "article-title": "Production of secretory IgA antibodies in plants",
      "volume": "18",
      "author": "Larrick",
      "year": "2001",
      "journal-title": "Biomol. Eng."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB28",
      "series-title": "Carbohydrates in Chemistry and Biology",
      "first-page": "1043",
      "article-title": "Metabolic engineering glycosylation: biotechnology's challenge to the glycobiologist in the new millennium",
      "author": "Warner",
      "year": "2000"
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "5739",
      "DOI": "10.1021/ja017881+",
      "article-title": "Efficient chemoenzymatic synthesis of O-linked sialyl oligosaccharides",
      "volume": "124",
      "author": "Blixt",
      "year": "2002",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB30",
      "doi-asserted-by": "crossref",
      "first-page": "2899",
      "DOI": "10.1073/pnas.031419998",
      "article-title": "Galactose-extended glycans of antibodies produced by transgenic plants",
      "volume": "98",
      "author": "Bakker",
      "year": "2001",
      "journal-title": "Proc. Natl. Acad. Sci. U. S. A."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "187",
      "DOI": "10.1023/A:1008976219939",
      "article-title": "A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice",
      "volume": "9",
      "author": "Chargelegue",
      "year": "2000",
      "journal-title": "Transgenic Res."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB32",
      "first-page": "161",
      "article-title": "Plant genes and their expression",
      "volume": "80",
      "author": "Tyagi",
      "year": "2001",
      "journal-title": "Curr. Sci."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB33",
      "doi-asserted-by": "crossref",
      "first-page": "213",
      "DOI": "10.1007/BF01969712",
      "article-title": "Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants",
      "volume": "5",
      "author": "Christensen",
      "year": "1996",
      "journal-title": "Transgenic Res."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB34",
      "doi-asserted-by": "crossref",
      "first-page": "489",
      "DOI": "10.1007/BF00232980",
      "article-title": "Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.)",
      "volume": "15",
      "author": "Vain",
      "year": "1996",
      "journal-title": "Plant Cell Rep."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB35",
      "doi-asserted-by": "crossref",
      "first-page": "146",
      "DOI": "10.1016/S0958-1669(00)00073-2",
      "article-title": "Chemical-inducible systems for regulated expression of plant genes",
      "volume": "11",
      "author": "Zuo",
      "year": "2000",
      "journal-title": "Curr. Opin. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB36",
      "first-page": "95",
      "article-title": "Transgenic plants for therapeutic proteins: linking upstream and downstream technologies",
      "volume": "240",
      "author": "Cramer",
      "year": "1999",
      "journal-title": "Curr. Top. Microbiol. Immunol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB37",
      "doi-asserted-by": "crossref",
      "first-page": "429",
      "DOI": "10.1016/S1360-1385(99)01484-3",
      "article-title": "Current perspectives on mRNA stability in plants: multiple levels and mechanisms of control",
      "volume": "4",
      "author": "Guti\u00e9rrez",
      "year": "1999",
      "journal-title": "Trends Plant Sci."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB38",
      "doi-asserted-by": "crossref",
      "first-page": "460",
      "DOI": "10.1016/S1369-5266(02)00290-X",
      "article-title": "Regulation of translational initiation in plants",
      "volume": "5",
      "author": "Kawaguchi",
      "year": "2002",
      "journal-title": "Curr. Opin. Plant Biol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB39",
      "doi-asserted-by": "crossref",
      "first-page": "393",
      "DOI": "10.1007/BF00039392",
      "article-title": "Optimizing expression of transgenes with an emphasis on post-transcriptional events",
      "volume": "32",
      "author": "Koziel",
      "year": "1996",
      "journal-title": "Plant Mol. Biol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB40",
      "doi-asserted-by": "crossref",
      "first-page": "562",
      "DOI": "10.1016/S0959-437X(00)00128-3",
      "article-title": "The silence of the genes",
      "volume": "10",
      "author": "Plasterk",
      "year": "2000",
      "journal-title": "Curr. Opin. Genet. Dev."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB41",
      "first-page": "107",
      "article-title": "Foreign DNA: integration and expression in transgenic plants",
      "volume": "Vol. 24",
      "author": "Twyman",
      "year": "2002"
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB42",
      "doi-asserted-by": "crossref",
      "first-page": "444",
      "DOI": "10.1016/S1369-5266(02)00291-1",
      "article-title": "RNA silencing: small RNAs as ubiquitous regulators of gene expression",
      "volume": "5",
      "author": "Voinnet",
      "year": "2002",
      "journal-title": "Curr. Opin. Plant Biol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB43",
      "doi-asserted-by": "crossref",
      "first-page": "247",
      "DOI": "10.1023/A:1023941407376",
      "article-title": "Transgene integration, organization and interaction in plants",
      "volume": "52",
      "author": "Kohli",
      "year": "2003",
      "journal-title": "Plant Mol. Biol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB44",
      "doi-asserted-by": "crossref",
      "first-page": "11",
      "DOI": "10.1023/A:1008993730505",
      "article-title": "Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns",
      "volume": "9",
      "author": "Fu",
      "year": "2000",
      "journal-title": "Transgenic Res."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB45",
      "doi-asserted-by": "crossref",
      "first-page": "369",
      "DOI": "10.1023/A:1009638600492",
      "article-title": "Intracellular expression of TMV-specific single-chain Fv fragments leads to improved virus resistance in Nicotiana tabacum",
      "volume": "4",
      "author": "Zimmermann",
      "year": "1998",
      "journal-title": "Mol. Breed."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB46",
      "doi-asserted-by": "crossref",
      "first-page": "255",
      "DOI": "10.1023/A:1008937011213",
      "article-title": "Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum",
      "volume": "8",
      "author": "Schillberg",
      "year": "1999",
      "journal-title": "Transgenic Res."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB47",
      "doi-asserted-by": "crossref",
      "first-page": "426",
      "DOI": "10.1046/j.1432-1327.1999.00060.x",
      "article-title": "High-level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida",
      "volume": "259",
      "author": "De Jaeger",
      "year": "1999",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB48",
      "doi-asserted-by": "crossref",
      "first-page": "19339",
      "DOI": "10.1074/jbc.M201245200",
      "article-title": "Formation of disulfide bridges by a single-chain Fv antibody in the reducing ectopic environment of the plant cytosol",
      "volume": "277",
      "author": "Schouten",
      "year": "2002",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB49",
      "doi-asserted-by": "crossref",
      "first-page": "6042",
      "DOI": "10.1046/j.1432-1033.2002.03302.x",
      "article-title": "ER-resident chaperone interactions with recombinant antibodies in transgenic plants",
      "volume": "269",
      "author": "Nuttall",
      "year": "2002",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB50",
      "doi-asserted-by": "crossref",
      "first-page": "101",
      "DOI": "10.1023/A:1006029617949",
      "article-title": "Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity",
      "volume": "38",
      "author": "Conrad",
      "year": "1998",
      "journal-title": "Plant Mol. Biol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB51",
      "unstructured": "Sriraman, R. et al. Glycosylation of recombinant antibodies in plants. Proceedings of the Ranbaxy Science Foundation Symposium 2002, Ranbaxy Science Foundation, New Delhi (in press)."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB52",
      "doi-asserted-by": "crossref",
      "first-page": "84",
      "DOI": "10.1016/S1360-1385(01)02193-8",
      "article-title": "Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology",
      "volume": "7",
      "author": "Daniell",
      "year": "2002",
      "journal-title": "Trends Plant Sci."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB53",
      "doi-asserted-by": "crossref",
      "first-page": "333",
      "DOI": "10.1038/73796",
      "article-title": "High-yield production of a human therapeutic protein in tobacco chloroplasts",
      "volume": "18",
      "author": "Staub",
      "year": "2000",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB54",
      "first-page": "77",
      "article-title": "A chloroplast transgenic approach to hyper-express and purify human serum albumin, a protein highly susceptible to proteolytic degradation",
      "volume": "1",
      "author": "Millan",
      "year": "2003",
      "journal-title": "Plant Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB55",
      "doi-asserted-by": "crossref",
      "first-page": "1001",
      "DOI": "10.1006/jmbi.2001.4921",
      "article-title": "Expression of the native cholera B toxin subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts",
      "volume": "311",
      "author": "Daniell",
      "year": "2001",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB56",
      "doi-asserted-by": "crossref",
      "first-page": "1174",
      "DOI": "10.1093/nar/gkg221",
      "article-title": "Expression of tetanus toxin fragment C in tobacco chloroplasts",
      "volume": "31",
      "author": "Tregoning",
      "year": "2003",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB57",
      "doi-asserted-by": "crossref",
      "first-page": "59",
      "DOI": "10.1023/A:1022168321380",
      "article-title": "Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme",
      "volume": "11",
      "author": "Leelavathi",
      "year": "2003",
      "journal-title": "Mol. Breed."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB58",
      "doi-asserted-by": "crossref",
      "first-page": "3345",
      "DOI": "10.1128/AEM.68.7.3345-3351.2002",
      "article-title": "In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria",
      "volume": "68",
      "author": "Kay",
      "year": "2002",
      "journal-title": "Appl. Environ. Microbiol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB59",
      "doi-asserted-by": "crossref",
      "first-page": "77",
      "DOI": "10.1038/nbt772",
      "article-title": "Immunomodulation of enzyme function in plants by single domain antibody fragments",
      "volume": "21",
      "author": "Jobling",
      "year": "2003",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB60",
      "doi-asserted-by": "crossref",
      "first-page": "149",
      "DOI": "10.1023/A:1019714614827",
      "article-title": "Practical considerations for pharmaceutical antibody production in different crop systems",
      "volume": "9",
      "author": "St\u00f6ger",
      "year": "2002",
      "journal-title": "Mol. Breed."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB61",
      "doi-asserted-by": "crossref",
      "unstructured": "D'Aoust, M.A. Perennial plants as a production system for pharmaceuticals. In Handbook of Plant Biotechnology (Christou, P. and Klee, H., eds), Wiley-VCH (in press).",
      "DOI": "10.1002/0470869143.kc043"
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB62",
      "doi-asserted-by": "crossref",
      "first-page": "1361",
      "DOI": "10.1038/4344",
      "article-title": "A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes",
      "volume": "16",
      "author": "Zeitlin",
      "year": "1998",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB63",
      "doi-asserted-by": "crossref",
      "first-page": "135",
      "DOI": "10.1002/(SICI)1097-0290(19990720)64:2<135::AID-BIT2>3.0.CO;2-Q",
      "article-title": "Production of a diagnostic monoclonal antibody in perennial alfalfa plants",
      "volume": "64",
      "author": "Khoudi",
      "year": "1999",
      "journal-title": "Biotechnol. Bioeng."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB64",
      "doi-asserted-by": "crossref",
      "first-page": "1796",
      "DOI": "10.1096/fasebj.13.13.1796",
      "article-title": "A plant-derived edible vaccine against hepatitis B virus",
      "volume": "13",
      "author": "Kapusta",
      "year": "1999",
      "journal-title": "FASEB J."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB65",
      "doi-asserted-by": "crossref",
      "first-page": "279",
      "DOI": "10.1016/S0141-0229(01)00502-6",
      "article-title": "From green plants to industrial enzymes",
      "volume": "30",
      "author": "Hood",
      "year": "2002",
      "journal-title": "Enzyme & Microbial Technol"
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB66",
      "first-page": "485",
      "article-title": "Microplate assay for soybean seed coat peroxidase activity",
      "volume": "24",
      "author": "Vierling",
      "year": "1996",
      "journal-title": "Seed Science Technol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB67",
      "doi-asserted-by": "crossref",
      "first-page": "607",
      "DOI": "10.1038/nm0598-607",
      "article-title": "Immunogenicity in humans of a recombinant bacterial-antigen delivered in transgenic potato",
      "volume": "4",
      "author": "Tacket",
      "year": "1998",
      "journal-title": "Nat. Med."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB68",
      "doi-asserted-by": "crossref",
      "first-page": "302",
      "DOI": "10.1086/315653",
      "article-title": "Human immune responses to a novel Norwalk virus vaccine delivered in transgenic potatoes",
      "volume": "182",
      "author": "Tacket",
      "year": "2000",
      "journal-title": "J. Infect. Dis."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB69",
      "doi-asserted-by": "crossref",
      "first-page": "1167",
      "DOI": "10.1038/81153",
      "article-title": "Production of hepatitis B surface antigen in transgenic plants for oral immunization",
      "volume": "18",
      "author": "Richter",
      "year": "2000",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB70",
      "doi-asserted-by": "crossref",
      "first-page": "163",
      "DOI": "10.1023/A:1022912130286",
      "article-title": "Expression of rotavirus capsid protein VP6 in transgenic potato and its oral immunogenicity in mice",
      "volume": "12",
      "author": "Yu",
      "year": "2003",
      "journal-title": "Transgenic Res."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB71",
      "doi-asserted-by": "crossref",
      "first-page": "313",
      "DOI": "10.1023/A:1009676832273",
      "article-title": "Potato tubers as a biofactory for recombinant antibodies",
      "volume": "4",
      "author": "Artsaenko",
      "year": "1998",
      "journal-title": "Mol. Breed."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB72",
      "doi-asserted-by": "crossref",
      "first-page": "2871",
      "DOI": "10.1023/A:1020306917914",
      "article-title": "Expression of antibodies and Fab fragments in transgenic potato plants: a case study for bulk production in crop plants",
      "volume": "9",
      "author": "De Wilde",
      "year": "2002",
      "journal-title": "Mol. Breed."
    },
    {
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      "DOI": "10.1023/A:1026752805494",
      "article-title": "Biopharming the SimpliRED\u2122 HIV diagnostic reagent in barley, potato and tobacco",
      "volume": "9",
      "author": "Schunmann",
      "year": "2002",
      "journal-title": "Mol. Breed."
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      "DOI": "10.1023/A:1008977630179",
      "article-title": "Expression of full-length bioactive antimicrobial human lactoferrin in potato plants",
      "volume": "9",
      "author": "Chong",
      "year": "2000",
      "journal-title": "Transgenic Res."
    },
    {
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      "doi-asserted-by": "crossref",
      "first-page": "289",
      "DOI": "10.1023/A:1018410712288",
      "article-title": "Expression of the human milk protein \u03b2-casein in transgenic potato plants",
      "volume": "6",
      "author": "Chong",
      "year": "1997",
      "journal-title": "Transgenic Res."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB76",
      "doi-asserted-by": "crossref",
      "first-page": "1484",
      "DOI": "10.1038/nbt1295-1484",
      "article-title": "Expression of the rabies virus glycoprotein in transgenic tomatoes",
      "volume": "13",
      "author": "McGarvey",
      "year": "1995",
      "journal-title": "Biotechnology (N.Y.)"
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB77",
      "doi-asserted-by": "crossref",
      "first-page": "803",
      "DOI": "10.1016/S0264-410X(02)00603-5",
      "article-title": "Vaccine antigen production in transgenic plants: strategies, gene constructs and perspectives",
      "volume": "21",
      "author": "Sala",
      "year": "2003",
      "journal-title": "Vaccine"
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      "first-page": "101",
      "DOI": "10.1016/S0168-9452(96)04541-4",
      "article-title": "An agrobacterium-mediated transient gene expression system for intact leaves",
      "volume": "122",
      "author": "Kapila",
      "year": "1997",
      "journal-title": "Plant Sci."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB79",
      "doi-asserted-by": "crossref",
      "first-page": "408",
      "DOI": "10.1096/fj.01-0363fje",
      "article-title": "A carcinoembryonic antigen-specific diabody produced in tobacco",
      "volume": "16",
      "author": "Vaquero",
      "year": "2002",
      "journal-title": "FASEB J."
    },
    {
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      "first-page": "69",
      "DOI": "10.1016/S0022-1759(98)00149-5",
      "article-title": "Expression and assembly of a full-length monoclonal antibody in plants using a plant virus vector",
      "volume": "220",
      "author": "Verch",
      "year": "1998",
      "journal-title": "J. Immunol. Methods"
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB81",
      "doi-asserted-by": "crossref",
      "first-page": "703",
      "DOI": "10.1073/pnas.96.2.703",
      "article-title": "Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants",
      "volume": "96",
      "author": "McCormick",
      "year": "1999",
      "journal-title": "Proc. Natl. Acad. Sci. U. S. A."
    },
    {
      "key": "10.1016/j.tibtech.2003.10.002_BIB82",
      "doi-asserted-by": "crossref",
      "unstructured": "Mor, T.S. and Mason, H.S. Plants as a source of subunit vaccines. In Handbook of Plant Biotechnology (Christou, P. and Klee, H., eds), Wiley-VCH (in press).",
      "DOI": "10.1002/0470869143.kc041"
    },
    {
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      "first-page": "199",
      "DOI": "10.1016/S0958-1669(00)00086-0",
      "article-title": "Foreign protein production in plant tissue cultures",
      "volume": "11",
      "author": "Doran",
      "year": "2000",
      "journal-title": "Curr. Opin. Biotechnol."
    },
    {
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      "first-page": "441",
      "DOI": "10.1023/A:1008969031219",
      "article-title": "Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies",
      "volume": "8",
      "author": "Torres",
      "year": "1999",
      "journal-title": "Transgenic Res."
    },
    {
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      "doi-asserted-by": "crossref",
      "first-page": "427",
      "DOI": "10.1093/glycob/cwg024",
      "article-title": "Immunoreactivity in mammals of two typical plant glyco-epitopes, core \u03b1(1,3)-fucose and core xylose",
      "volume": "13",
      "author": "Bardor",
      "year": "2003",
      "journal-title": "Glycobiology"
    },
    {
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      "first-page": "31",
      "DOI": "10.1023/A:1006012005654",
      "article-title": "N-glycoprotein biosynthesis in plants: recent developments and future trends",
      "volume": "38",
      "author": "Lerouge",
      "year": "1998",
      "journal-title": "Plant Mol. Biol."
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