DOI: 10.1016/j.tibs.2014.10.005. How cryo-EM is revolutionizing structural biology

How cryo-EM is revolutionizing structural biology

10.1016/j.tibs.2014.10.005

Crossref journal-article

Elsevier BV

Trends in Biochemical Sciences (78)

Bibliography

Bai, X., McMullan, G., & Scheres, S. H. W. (2015). How cryo-EM is revolutionizing structural biology. Trends in Biochemical Sciences, 40(1), 49â57.

Authors 3

Xiao-chen Bai (first)
Greg McMullan (additional)
Sjors H.W Scheres (additional)

References 93 Referenced 801

10.1007/BF01479367 / Naturwissenschaften / Über ein neues bei der bakteriophagen Lyse auftretendes Formelement by Ruska (1941)
10.1007/BF01243399 / Arch. Für Gesamte Virusforsch. / Die Bedeutung der Übermikroskopie für die Virusforschung by Ruska (1939)
10.1016/0006-3002(59)90237-9 / Biochim. Biophys. Acta / A negative staining method for high resolution electron microscopy of viruses by Brenner (1959)
10.1038/257028a0 / Nature / Three-dimensional model of purple membrane obtained by electron microscopy by Henderson (1975)
10.1016/0022-2836(75)90212-0 / J. Mol. Biol. / Molecular structure determination by electron microscopy of unstained crystalline specimens by Unwin (1975)
10.1126/science.186.4168.1036 / Science / Electron diffraction of frozen, hydrated protein crystals by Taylor (1974)
10.1016/S0304-3991(79)90211-0 / Ultramicroscopy / Radiation damage of purple membrane at low temperature by Hayward (1979)
10.1038/308032a0 / Nature / Cryo-electron microscopy of viruses by Adrian (1984)
10.1146/annurev.bb.10.060181.001025 / Annu. Rev. Biophys. Bioeng. / Low temperature electron microscopy by Dubochet (1981)
10.1017/S0033583500004297 / Q. Rev. Biophys. / Cryo-electron microscopy of vitrified specimens by Dubochet (1988)
10.1038/34465 / Nature / Structure of the alpha beta tubulin dimer by electron crystallography by Nogales (1998)
10.1038/367614a0 / Nature / Atomic model of plant light-harvesting complex by electron crystallography by Kühlbrandt (1994)
10.1038/nature04321 / Nature / Lipid-protein interactions in double-layered two-dimensional AQP0 crystals by Gonen (2005)
10.1016/j.jmb.2004.12.031 / J. Mol. Biol. / Refined structure of the nicotinic acetylcholine receptor at 4A resolution by Unwin (2005)
10.1016/0304-3991(93)90246-T / Ultramicroscopy / Prospects for using an IVEM with a FEG for imaging macromolecules towards atomic resolution by Zhou (1993)
10.1017/S003358350000305X / Q. Rev. Biophys. / The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules by Henderson (1995)
10.1038/386088a0 / Nature / Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy by Böttcher (1997)
10.1038/386091a0 / Nature / Visualization of a 4-helix bundle in the hepatitis B virus capsid by cryo-electron microscopy by Conway (1997)
10.1038/nature06665 / Nature / Backbone structure of the infectious epsilon15 virus capsid revealed by electron cryomicroscopy by Jiang (2008)
10.1038/nature06893 / Nature / 3.88 A structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy by Yu (2008)
10.1073/pnas.0711623105 / Proc. Natl. Acad. Sci. U.S.A. / Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction by Zhang (2008)
10.1016/j.cell.2010.03.041 / Cell / 3.3Å cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry by Zhang (2010)
10.1126/science.1187433 / Science / Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks by Liu (2010)
10.1016/0022-2836(84)90245-6 / J. Mol. Biol. / Three-dimensional reconstruction of the 30 S ribosomal subunit from randomly oriented particles by Verschoor (1984)
10.1002/j.1460-2075.1987.tb04865.x / EMBO J. / Three-dimensional structure of the large ribosomal subunit from Escherichia coli by Radermacher (1987)
10.1126/science.7313694 / Science / Computer averaging of electron micrographs of 40S ribosomal subunits by Frank (1981)
{'key': '10.1016/j.tibs.2014.10.005_bib0135', 'first-page': '187', 'article-title': 'Use of multivariate statistics in analysing the images of biological macromolecules', 'volume': '6', 'author': 'Van Heel', 'year': '1981', 'journal-title': 'Ultramicroscopy'} / Ultramicroscopy / Use of multivariate statistics in analysing the images of biological macromolecules by Van Heel (1981)
{'key': '10.1016/j.tibs.2014.10.005_bib0140', 'first-page': '146', 'article-title': 'Exact filters for general geometry three dimensional reconstruction', 'volume': '73', 'author': 'Harauz', 'year': '1986', 'journal-title': 'Optik'} / Optik / Exact filters for general geometry three dimensional reconstruction by Harauz (1986)
{'key': '10.1016/j.tibs.2014.10.005_bib0145', 'first-page': '125', 'article-title': 'On the correction of the contrast transfer function in biological electron microscopy', 'volume': '98', 'author': 'Frank', 'year': '1987', 'journal-title': 'Optik'} / Optik / On the correction of the contrast transfer function in biological electron microscopy by Frank (1987)
{'key': '10.1016/j.tibs.2014.10.005_bib0150', 'series-title': 'Three-dimensional Electron Microscopy of Macromolecular Assemblies', 'author': 'Frank', 'year': '2006'} / Three-dimensional Electron Microscopy of Macromolecular Assemblies by Frank (2006)
10.1083/jcb.115.3.597 / J. Cell Biol. / Three-dimensional reconstruction of the 70S Escherichia coli ribosome in ice: the distribution of ribosomal RNA by Frank (1991)
10.1016/S0092-8674(00)80690-X / Cell / Solution structure of the E. coli 70S ribosome at 11.5Å resolution by Gabashvili (2000)
10.1126/science.1251652 / Science / Biochemistry. The resolution revolution by Kühlbrandt (2014)
{'key': '10.1016/j.tibs.2014.10.005_bib0170', 'series-title': 'Image Science: Principles, Analysis and Evaluation of Photographic-type Imaging Processes', 'author': 'Dainty', 'year': '1974'} / Image Science: Principles, Analysis and Evaluation of Photographic-type Imaging Processes by Dainty (1974)
10.1364/JOSA.57.000232 / J. Opt. Soc. Am. / Image recording in electron microscopy by Hamilton (1967)
10.1016/j.ultramic.2009.04.002 / Ultramicroscopy / Detective quantum efficiency of electron area detectors in electron microscopy by McMullan (2009)
10.1016/0304-3991(82)90061-4 / Ultramicroscopy / A CCD-based image recording system for the CTEM by Roberts (1982)
10.1063/1.1140039 / Rev. Sci. Instrum. / Large dynamic range, parallel detection system for electron diffraction and imaging by Spence (1988)
10.1016/0304-3991(93)90216-K / Ultramicroscopy / Applications of slow-scan CCD cameras in transmission electron microscopy by Krivanek (1993)
10.1016/S0304-3991(98)00056-4 / Ultramicroscopy / Cooled CCD detector with tapered fibre optics for recording electron diffraction patterns by Faruqi (1999)
10.1016/j.jsb.2005.03.010 / J. Struct. Biol. / Automated molecular microscopy: the new Leginon system by Suloway (2005)
10.1016/j.jsb.2005.04.004 / J. Struct. Biol. / Advantages of CCD detectors for de novo three-dimensional structure determination in single-particle electron microscopy by Sander (2005)
10.1016/j.jsb.2004.02.004 / J. Struct. Biol. / A 9 angstroms single particle reconstruction from CCD captured images on a 200kV electron cryomicroscope by Booth (2004)
10.1017/S0033583511000035 / Q. Rev. Biophys. / Electronic detectors for electron microscopy by Faruqi (2011)
10.1016/S0168-9002(00)00893-7 / Nucl. Instrum. Methods Phys. Res. Sect. Accel. Spectrometers Detect. Assoc. Equip. / A monolithic active pixel sensor for charged particle tracking and imaging using standard VLSI CMOS technology by Turchetta (2001)
10.1016/j.ultramic.2005.03.006 / Ultramicroscopy / Active pixel sensor array as a detector for electron microscopy by Milazzo (2005)
10.1016/j.nima.2005.03.023 / Nucl. Instrum. Methods Phys. Res. Sect. Accel. Spectrometers Detect. Assoc. Equip. / Direct single electron detection with a CMOS detector for electron microscopy by Faruqi (2005)
10.1016/j.ultramic.2009.05.005 / Ultramicroscopy / Experimental observation of the improvement in MTF from backthinning a CMOS direct electron detector by McMullan (2009)
10.1016/j.jsb.2013.10.016 / J. Struct. Biol. / Quantitative characterization of electron detectors for transmission electron microscopy by Ruskin (2013)
10.1016/j.ultramic.2014.08.002 / Ultramicroscopy / Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy by McMullan (2014)
10.1103/RevModPhys.60.663 / Rev. Mod. Phys. / Straggling in thin silicon detectors by Bichsel (1988)
10.1016/j.ultramic.2009.07.004 / Ultramicroscopy / Enhanced imaging in low dose electron microscopy using electron counting by McMullan (2009)
10.1038/nmeth.2472 / Nat. Methods / Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM by Li (2013)
10.1016/j.jsb.2013.08.005 / J. Struct. Biol. / Influence of electron dose rate on electron counting images recorded with the K2 camera by Li (2013)
10.1016/j.jsb.2004.02.008 / J. Struct. Biol. / Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset by Gao (2004)
10.1093/emboj/cdf326 / EMBO J. / Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process by Valle (2002)
10.1006/jsbi.1998.4014 / J. Struct. Biol. / A maximum-likelihood approach to single-particle image refinement by Sigworth (1998)
10.1038/nmeth992 / Nat. Methods / Disentangling conformational states of macromolecules in 3D-EM through likelihood optimization by Scheres (2007)
10.1016/j.jsb.2007.11.007 / J. Struct. Biol. / Detection and separation of heterogeneity in molecular complexes by statistical analysis of their two-dimensional projections by Elad (2008)
10.1016/j.str.2008.10.011 / Structure / Heterogeneity of large macromolecular complexes revealed by 3D cryo-EM variance analysis by Zhang (2008)
10.1016/j.sbi.2009.08.001 / Curr. Opin. Struct. Biol. / Exploring conformational modes of macromolecular assemblies by multiparticle cryo-EM by Spahn (2009)
10.1016/j.jsb.2013.07.005 / J. Struct. Biol. / Likelihood-based classification of cryo-EM images using FREALIGN by Lyumkis (2013)
10.1016/j.jmb.2011.11.010 / J. Mol. Biol. / A Bayesian view on cryo-EM structure determination by Scheres (2012)
10.1016/j.jsb.2012.09.006 / J. Struct. Biol. / RELION: Implementation of a Bayesian approach to cryo-EM structure determination by Scheres (2012)
10.1016/j.jsb.2012.02.003 / J. Struct. Biol. / Beam-induced motion of vitrified specimen on holey carbon film by Brilot (2012)
10.1016/j.str.2012.08.026 / Structure / Movies of ice-embedded particles enhance resolution in electron cryo-microscopy by Campbell (2012)
10.7554/eLife.00461 / Elife / Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles by Bai (2013)
10.1038/nature12823 / Nature / TRPV1 structures in distinct conformations reveal activation mechanisms by Cao (2013)
10.1038/nature12822 / Nature / Structure of the TRPV1 ion channel determined by electron cryo-microscopy by Liao (2013)
10.7554/eLife.01963 / Elife / Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector by Allegretti (2014)
10.1126/science.1249410 / Science / Structure of the yeast mitochondrial large ribosomal subunit by Amunts (2014)
10.1107/S0907444910007493 / Acta Crystallogr. D: Biol. Crystallogr. / Features and development of Coot by Emsley (2010)
10.1107/S0907444996012255 / Acta Crystallogr. Sect. D / Refinement of macromolecular structures by the maximum-likelihood method by Murshudov (1997)
10.1002/pro.2267 / Protein Sci. / Cryo-EM model validation using independent map reconstructions by DiMaio (2013)
10.1073/pnas.1119041110 / Proc. Natl. Acad. Sci. U.S.A. / Cross-validation in cryo-EM-based structural modeling by Falkner (2013)
10.7554/eLife.03080 / Elife / Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine by Wong (2014)
10.1038/nature13567 / Nature / Three-dimensional structure of human γ-secretase by Lu (2014)
10.7554/eLife.03665 / Elife / Beam-induced motion correction for sub-megadalton cryo-EM particles by Scheres (2014)
10.1126/science.1240585 / Science / Molecular architecture of a eukaryotic translational initiation complex by Fernández (2013)
10.1073/pnas.1406335111 / Proc. Natl. Acad. Sci. U.S.A. / Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA-like structural element in the ribosomal decoding center by Koh (2014)
10.1016/j.cell.2014.04.015 / Cell / Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome by Fernández (2014)
10.1016/0304-3991(96)00045-9 / Ultramicroscopy / Electron radiation damage to protein crystals of bacteriorhodopsin at different temperatures by Stark (1996)
10.1016/j.jsb.2013.04.014 / J. Struct. Biol. / Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: reduction of electron-optical effects of charging by Sader (2013)
10.1038/nmeth.2931 / Nat. Methods / Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas by Russo (2014)
10.1016/j.jsb.2013.11.002 / J. Struct. Biol. / Fabrication of carbon films with ∼500nm holes for cryo-EM with a direct detector device by Marr (2014)
10.1016/j.ultramic.2012.03.006 / Ultramicroscopy / Paraxial charge compensator for electron cryomicroscopy by Berriman (2012)
10.1016/j.jsb.2006.05.004 / J. Struct. Biol. / FREALIGN: high-resolution refinement of single particle structures by Grigorieff (2007)
10.1006/jsbi.1999.4174 / J. Struct. Biol. / EMAN: semiautomated software for high-resolution single-particle reconstructions by Ludtke (1999)
10.1016/j.jsb.2007.06.009 / J. Struct. Biol. / Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a Multi-Path Simulated Annealing optimization algorithm by Liu (2007)
10.1128/JVI.02641-13 / J. Virol. / Near-atomic resolution cryo-electron microscopic structure of dengue serotype 4 virus by Kostyuchenko (2014)
10.1073/pnas.1300601110 / Proc. Natl. Acad. Sci. U.S.A. / Atomic structure of the 75MDa extremophile Sulfolobus turreted icosahedral virus determined by CryoEM and X-ray crystallography by Veesler (2013)
10.1006/jsbi.1996.0004 / J. Struct. Biol. / A new generation of the IMAGIC image processing system by Van Heel (1996)
10.1038/nature12890 / Nature / Architecture of the large subunit of the mammalian mitochondrial ribosome by Greber (2013)

Dates

Type	When
Created	10 years, 9 months ago (Nov. 7, 2014, 1:14 a.m.)
Deposited	6 years ago (Aug. 16, 2019, 11:53 p.m.)
Indexed	3 days ago (Aug. 30, 2025, 12:27 p.m.)
Issued	10 years, 8 months ago (Jan. 1, 2015)
Published	10 years, 8 months ago (Jan. 1, 2015)
Published Print	10 years, 8 months ago (Jan. 1, 2015)

Funders 0

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BibTeX

@article{Bai_2015, title={How cryo-EM is revolutionizing structural biology}, volume={40}, ISSN={0968-0004}, url={http://dx.doi.org/10.1016/j.tibs.2014.10.005}, DOI={10.1016/j.tibs.2014.10.005}, number={1}, journal={Trends in Biochemical Sciences}, publisher={Elsevier BV}, author={Bai, Xiao-chen and McMullan, Greg and Scheres, Sjors H.W}, year={2015}, month=jan, pages={49–57} }

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      "article-title": "A negative staining method for high resolution electron microscopy of viruses",
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      "author": "Henderson",
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      "first-page": "425",
      "DOI": "10.1016/0022-2836(75)90212-0",
      "article-title": "Molecular structure determination by electron microscopy of unstained crystalline specimens",
      "volume": "94",
      "author": "Unwin",
      "year": "1975",
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      "author": "Taylor",
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      "author": "Hayward",
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    {
      "key": "10.1016/j.tibs.2014.10.005_bib0040",
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      "first-page": "32",
      "DOI": "10.1038/308032a0",
      "article-title": "Cryo-electron microscopy of viruses",
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      "author": "Adrian",
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      "journal-title": "Nature"
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    {
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      "first-page": "133",
      "DOI": "10.1146/annurev.bb.10.060181.001025",
      "article-title": "Low temperature electron microscopy",
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      "author": "Dubochet",
      "year": "1981",
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      "article-title": "Cryo-electron microscopy of vitrified specimens",
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      "author": "Dubochet",
      "year": "1988",
      "journal-title": "Q. Rev. Biophys."
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    {
      "key": "10.1016/j.tibs.2014.10.005_bib0055",
      "doi-asserted-by": "crossref",
      "first-page": "199",
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      "author": "Nogales",
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      "first-page": "614",
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      "author": "K\u00fchlbrandt",
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      "journal-title": "Nature"
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      "first-page": "633",
      "DOI": "10.1038/nature04321",
      "article-title": "Lipid-protein interactions in double-layered two-dimensional AQP0 crystals",
      "volume": "438",
      "author": "Gonen",
      "year": "2005",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0070",
      "doi-asserted-by": "crossref",
      "first-page": "967",
      "DOI": "10.1016/j.jmb.2004.12.031",
      "article-title": "Refined structure of the nicotinic acetylcholine receptor at 4A resolution",
      "volume": "346",
      "author": "Unwin",
      "year": "2005",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0075",
      "doi-asserted-by": "crossref",
      "first-page": "407",
      "DOI": "10.1016/0304-3991(93)90246-T",
      "article-title": "Prospects for using an IVEM with a FEG for imaging macromolecules towards atomic resolution",
      "volume": "49",
      "author": "Zhou",
      "year": "1993",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0080",
      "doi-asserted-by": "crossref",
      "first-page": "171",
      "DOI": "10.1017/S003358350000305X",
      "article-title": "The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules",
      "volume": "28",
      "author": "Henderson",
      "year": "1995",
      "journal-title": "Q. Rev. Biophys."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0085",
      "doi-asserted-by": "crossref",
      "first-page": "88",
      "DOI": "10.1038/386088a0",
      "article-title": "Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy",
      "volume": "386",
      "author": "B\u00f6ttcher",
      "year": "1997",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0090",
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      "first-page": "91",
      "DOI": "10.1038/386091a0",
      "article-title": "Visualization of a 4-helix bundle in the hepatitis B virus capsid by cryo-electron microscopy",
      "volume": "386",
      "author": "Conway",
      "year": "1997",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0095",
      "doi-asserted-by": "crossref",
      "first-page": "1130",
      "DOI": "10.1038/nature06665",
      "article-title": "Backbone structure of the infectious epsilon15 virus capsid revealed by electron cryomicroscopy",
      "volume": "451",
      "author": "Jiang",
      "year": "2008",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0100",
      "doi-asserted-by": "crossref",
      "first-page": "415",
      "DOI": "10.1038/nature06893",
      "article-title": "3.88 A structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy",
      "volume": "453",
      "author": "Yu",
      "year": "2008",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0105",
      "doi-asserted-by": "crossref",
      "first-page": "1867",
      "DOI": "10.1073/pnas.0711623105",
      "article-title": "Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction",
      "volume": "105",
      "author": "Zhang",
      "year": "2008",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0110",
      "doi-asserted-by": "crossref",
      "first-page": "472",
      "DOI": "10.1016/j.cell.2010.03.041",
      "article-title": "3.3\u00c5 cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry",
      "volume": "141",
      "author": "Zhang",
      "year": "2010",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0115",
      "doi-asserted-by": "crossref",
      "first-page": "1038",
      "DOI": "10.1126/science.1187433",
      "article-title": "Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks",
      "volume": "329",
      "author": "Liu",
      "year": "2010",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0120",
      "doi-asserted-by": "crossref",
      "first-page": "677",
      "DOI": "10.1016/0022-2836(84)90245-6",
      "article-title": "Three-dimensional reconstruction of the 30 S ribosomal subunit from randomly oriented particles",
      "volume": "178",
      "author": "Verschoor",
      "year": "1984",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0125",
      "doi-asserted-by": "crossref",
      "first-page": "1107",
      "DOI": "10.1002/j.1460-2075.1987.tb04865.x",
      "article-title": "Three-dimensional structure of the large ribosomal subunit from Escherichia coli",
      "volume": "6",
      "author": "Radermacher",
      "year": "1987",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0130",
      "doi-asserted-by": "crossref",
      "first-page": "1353",
      "DOI": "10.1126/science.7313694",
      "article-title": "Computer averaging of electron micrographs of 40S ribosomal subunits",
      "volume": "214",
      "author": "Frank",
      "year": "1981",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0135",
      "first-page": "187",
      "article-title": "Use of multivariate statistics in analysing the images of biological macromolecules",
      "volume": "6",
      "author": "Van Heel",
      "year": "1981",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0140",
      "first-page": "146",
      "article-title": "Exact filters for general geometry three dimensional reconstruction",
      "volume": "73",
      "author": "Harauz",
      "year": "1986",
      "journal-title": "Optik"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0145",
      "first-page": "125",
      "article-title": "On the correction of the contrast transfer function in biological electron microscopy",
      "volume": "98",
      "author": "Frank",
      "year": "1987",
      "journal-title": "Optik"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0150",
      "series-title": "Three-dimensional Electron Microscopy of Macromolecular Assemblies",
      "author": "Frank",
      "year": "2006"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0155",
      "doi-asserted-by": "crossref",
      "first-page": "597",
      "DOI": "10.1083/jcb.115.3.597",
      "article-title": "Three-dimensional reconstruction of the 70S Escherichia coli ribosome in ice: the distribution of ribosomal RNA",
      "volume": "115",
      "author": "Frank",
      "year": "1991",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0160",
      "doi-asserted-by": "crossref",
      "first-page": "537",
      "DOI": "10.1016/S0092-8674(00)80690-X",
      "article-title": "Solution structure of the E. coli 70S ribosome at 11.5\u00c5 resolution",
      "volume": "100",
      "author": "Gabashvili",
      "year": "2000",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0165",
      "doi-asserted-by": "crossref",
      "first-page": "1443",
      "DOI": "10.1126/science.1251652",
      "article-title": "Biochemistry. The resolution revolution",
      "volume": "343",
      "author": "K\u00fchlbrandt",
      "year": "2014",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0170",
      "series-title": "Image Science: Principles, Analysis and Evaluation of Photographic-type Imaging Processes",
      "author": "Dainty",
      "year": "1974"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0175",
      "doi-asserted-by": "crossref",
      "first-page": "232",
      "DOI": "10.1364/JOSA.57.000232",
      "article-title": "Image recording in electron microscopy",
      "volume": "57",
      "author": "Hamilton",
      "year": "1967",
      "journal-title": "J. Opt. Soc. Am."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0180",
      "doi-asserted-by": "crossref",
      "first-page": "1126",
      "DOI": "10.1016/j.ultramic.2009.04.002",
      "article-title": "Detective quantum efficiency of electron area detectors in electron microscopy",
      "volume": "109",
      "author": "McMullan",
      "year": "2009",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0185",
      "doi-asserted-by": "crossref",
      "first-page": "385",
      "DOI": "10.1016/0304-3991(82)90061-4",
      "article-title": "A CCD-based image recording system for the CTEM",
      "volume": "8",
      "author": "Roberts",
      "year": "1982",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0190",
      "doi-asserted-by": "crossref",
      "first-page": "2102",
      "DOI": "10.1063/1.1140039",
      "article-title": "Large dynamic range, parallel detection system for electron diffraction and imaging",
      "volume": "59",
      "author": "Spence",
      "year": "1988",
      "journal-title": "Rev. Sci. Instrum."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0195",
      "doi-asserted-by": "crossref",
      "first-page": "95",
      "DOI": "10.1016/0304-3991(93)90216-K",
      "article-title": "Applications of slow-scan CCD cameras in transmission electron microscopy",
      "volume": "49",
      "author": "Krivanek",
      "year": "1993",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0200",
      "doi-asserted-by": "crossref",
      "first-page": "235",
      "DOI": "10.1016/S0304-3991(98)00056-4",
      "article-title": "Cooled CCD detector with tapered fibre optics for recording electron diffraction patterns",
      "volume": "75",
      "author": "Faruqi",
      "year": "1999",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0205",
      "doi-asserted-by": "crossref",
      "first-page": "41",
      "DOI": "10.1016/j.jsb.2005.03.010",
      "article-title": "Automated molecular microscopy: the new Leginon system",
      "volume": "151",
      "author": "Suloway",
      "year": "2005",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0210",
      "doi-asserted-by": "crossref",
      "first-page": "92",
      "DOI": "10.1016/j.jsb.2005.04.004",
      "article-title": "Advantages of CCD detectors for de novo three-dimensional structure determination in single-particle electron microscopy",
      "volume": "151",
      "author": "Sander",
      "year": "2005",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0215",
      "doi-asserted-by": "crossref",
      "first-page": "116",
      "DOI": "10.1016/j.jsb.2004.02.004",
      "article-title": "A 9 angstroms single particle reconstruction from CCD captured images on a 200kV electron cryomicroscope",
      "volume": "147",
      "author": "Booth",
      "year": "2004",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0220",
      "doi-asserted-by": "crossref",
      "first-page": "357",
      "DOI": "10.1017/S0033583511000035",
      "article-title": "Electronic detectors for electron microscopy",
      "volume": "44",
      "author": "Faruqi",
      "year": "2011",
      "journal-title": "Q. Rev. Biophys."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0225",
      "doi-asserted-by": "crossref",
      "first-page": "677",
      "DOI": "10.1016/S0168-9002(00)00893-7",
      "article-title": "A monolithic active pixel sensor for charged particle tracking and imaging using standard VLSI CMOS technology",
      "volume": "458",
      "author": "Turchetta",
      "year": "2001",
      "journal-title": "Nucl. Instrum. Methods Phys. Res. Sect. Accel. Spectrometers Detect. Assoc. Equip."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0230",
      "doi-asserted-by": "crossref",
      "first-page": "152",
      "DOI": "10.1016/j.ultramic.2005.03.006",
      "article-title": "Active pixel sensor array as a detector for electron microscopy",
      "volume": "104",
      "author": "Milazzo",
      "year": "2005",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0235",
      "doi-asserted-by": "crossref",
      "first-page": "170",
      "DOI": "10.1016/j.nima.2005.03.023",
      "article-title": "Direct single electron detection with a CMOS detector for electron microscopy",
      "volume": "546",
      "author": "Faruqi",
      "year": "2005",
      "journal-title": "Nucl. Instrum. Methods Phys. Res. Sect. Accel. Spectrometers Detect. Assoc. Equip."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0240",
      "doi-asserted-by": "crossref",
      "first-page": "1144",
      "DOI": "10.1016/j.ultramic.2009.05.005",
      "article-title": "Experimental observation of the improvement in MTF from backthinning a CMOS direct electron detector",
      "volume": "109",
      "author": "McMullan",
      "year": "2009",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0245",
      "doi-asserted-by": "crossref",
      "first-page": "385",
      "DOI": "10.1016/j.jsb.2013.10.016",
      "article-title": "Quantitative characterization of electron detectors for transmission electron microscopy",
      "volume": "184",
      "author": "Ruskin",
      "year": "2013",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0250",
      "doi-asserted-by": "crossref",
      "first-page": "156",
      "DOI": "10.1016/j.ultramic.2014.08.002",
      "article-title": "Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy",
      "volume": "147C",
      "author": "McMullan",
      "year": "2014",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0255",
      "doi-asserted-by": "crossref",
      "first-page": "663",
      "DOI": "10.1103/RevModPhys.60.663",
      "article-title": "Straggling in thin silicon detectors",
      "volume": "60",
      "author": "Bichsel",
      "year": "1988",
      "journal-title": "Rev. Mod. Phys."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0260",
      "doi-asserted-by": "crossref",
      "first-page": "1411",
      "DOI": "10.1016/j.ultramic.2009.07.004",
      "article-title": "Enhanced imaging in low dose electron microscopy using electron counting",
      "volume": "109",
      "author": "McMullan",
      "year": "2009",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0265",
      "doi-asserted-by": "crossref",
      "first-page": "584",
      "DOI": "10.1038/nmeth.2472",
      "article-title": "Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM",
      "volume": "10",
      "author": "Li",
      "year": "2013",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0270",
      "doi-asserted-by": "crossref",
      "first-page": "251",
      "DOI": "10.1016/j.jsb.2013.08.005",
      "article-title": "Influence of electron dose rate on electron counting images recorded with the K2 camera",
      "volume": "184",
      "author": "Li",
      "year": "2013",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0275",
      "doi-asserted-by": "crossref",
      "first-page": "283",
      "DOI": "10.1016/j.jsb.2004.02.008",
      "article-title": "Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset",
      "volume": "147",
      "author": "Gao",
      "year": "2004",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0280",
      "doi-asserted-by": "crossref",
      "first-page": "3557",
      "DOI": "10.1093/emboj/cdf326",
      "article-title": "Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process",
      "volume": "21",
      "author": "Valle",
      "year": "2002",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0285",
      "doi-asserted-by": "crossref",
      "first-page": "328",
      "DOI": "10.1006/jsbi.1998.4014",
      "article-title": "A maximum-likelihood approach to single-particle image refinement",
      "volume": "122",
      "author": "Sigworth",
      "year": "1998",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0290",
      "doi-asserted-by": "crossref",
      "first-page": "27",
      "DOI": "10.1038/nmeth992",
      "article-title": "Disentangling conformational states of macromolecules in 3D-EM through likelihood optimization",
      "volume": "4",
      "author": "Scheres",
      "year": "2007",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0295",
      "doi-asserted-by": "crossref",
      "first-page": "108",
      "DOI": "10.1016/j.jsb.2007.11.007",
      "article-title": "Detection and separation of heterogeneity in molecular complexes by statistical analysis of their two-dimensional projections",
      "volume": "162",
      "author": "Elad",
      "year": "2008",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0300",
      "doi-asserted-by": "crossref",
      "first-page": "1770",
      "DOI": "10.1016/j.str.2008.10.011",
      "article-title": "Heterogeneity of large macromolecular complexes revealed by 3D cryo-EM variance analysis",
      "volume": "16",
      "author": "Zhang",
      "year": "2008",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0305",
      "doi-asserted-by": "crossref",
      "first-page": "623",
      "DOI": "10.1016/j.sbi.2009.08.001",
      "article-title": "Exploring conformational modes of macromolecular assemblies by multiparticle cryo-EM",
      "volume": "19",
      "author": "Spahn",
      "year": "2009",
      "journal-title": "Curr. Opin. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0310",
      "doi-asserted-by": "crossref",
      "first-page": "377",
      "DOI": "10.1016/j.jsb.2013.07.005",
      "article-title": "Likelihood-based classification of cryo-EM images using FREALIGN",
      "volume": "183",
      "author": "Lyumkis",
      "year": "2013",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0315",
      "doi-asserted-by": "crossref",
      "first-page": "406",
      "DOI": "10.1016/j.jmb.2011.11.010",
      "article-title": "A Bayesian view on cryo-EM structure determination",
      "volume": "415",
      "author": "Scheres",
      "year": "2012",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0320",
      "doi-asserted-by": "crossref",
      "first-page": "519",
      "DOI": "10.1016/j.jsb.2012.09.006",
      "article-title": "RELION: Implementation of a Bayesian approach to cryo-EM structure determination",
      "volume": "180",
      "author": "Scheres",
      "year": "2012",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0325",
      "doi-asserted-by": "crossref",
      "first-page": "630",
      "DOI": "10.1016/j.jsb.2012.02.003",
      "article-title": "Beam-induced motion of vitrified specimen on holey carbon film",
      "volume": "177",
      "author": "Brilot",
      "year": "2012",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0330",
      "doi-asserted-by": "crossref",
      "first-page": "1823",
      "DOI": "10.1016/j.str.2012.08.026",
      "article-title": "Movies of ice-embedded particles enhance resolution in electron cryo-microscopy",
      "volume": "20",
      "author": "Campbell",
      "year": "2012",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0335",
      "doi-asserted-by": "crossref",
      "first-page": "e00461",
      "DOI": "10.7554/eLife.00461",
      "article-title": "Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles",
      "volume": "2",
      "author": "Bai",
      "year": "2013",
      "journal-title": "Elife"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0340",
      "doi-asserted-by": "crossref",
      "first-page": "113",
      "DOI": "10.1038/nature12823",
      "article-title": "TRPV1 structures in distinct conformations reveal activation mechanisms",
      "volume": "504",
      "author": "Cao",
      "year": "2013",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0345",
      "doi-asserted-by": "crossref",
      "first-page": "107",
      "DOI": "10.1038/nature12822",
      "article-title": "Structure of the TRPV1 ion channel determined by electron cryo-microscopy",
      "volume": "504",
      "author": "Liao",
      "year": "2013",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0350",
      "doi-asserted-by": "crossref",
      "first-page": "e01963",
      "DOI": "10.7554/eLife.01963",
      "article-title": "Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector",
      "volume": "3",
      "author": "Allegretti",
      "year": "2014",
      "journal-title": "Elife"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0355",
      "doi-asserted-by": "crossref",
      "first-page": "1485",
      "DOI": "10.1126/science.1249410",
      "article-title": "Structure of the yeast mitochondrial large ribosomal subunit",
      "volume": "343",
      "author": "Amunts",
      "year": "2014",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0360",
      "doi-asserted-by": "crossref",
      "first-page": "486",
      "DOI": "10.1107/S0907444910007493",
      "article-title": "Features and development of Coot",
      "volume": "66",
      "author": "Emsley",
      "year": "2010",
      "journal-title": "Acta Crystallogr. D: Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0365",
      "doi-asserted-by": "crossref",
      "first-page": "240",
      "DOI": "10.1107/S0907444996012255",
      "article-title": "Refinement of macromolecular structures by the maximum-likelihood method",
      "volume": "53",
      "author": "Murshudov",
      "year": "1997",
      "journal-title": "Acta Crystallogr. Sect. D"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0370",
      "doi-asserted-by": "crossref",
      "first-page": "865",
      "DOI": "10.1002/pro.2267",
      "article-title": "Cryo-EM model validation using independent map reconstructions",
      "volume": "22",
      "author": "DiMaio",
      "year": "2013",
      "journal-title": "Protein Sci."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0375",
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      "first-page": "8930",
      "DOI": "10.1073/pnas.1119041110",
      "article-title": "Cross-validation in cryo-EM-based structural modeling",
      "volume": "110",
      "author": "Falkner",
      "year": "2013",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0380",
      "doi-asserted-by": "crossref",
      "DOI": "10.7554/eLife.03080",
      "article-title": "Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine",
      "author": "Wong",
      "year": "2014",
      "journal-title": "Elife"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0385",
      "doi-asserted-by": "crossref",
      "first-page": "166",
      "DOI": "10.1038/nature13567",
      "article-title": "Three-dimensional structure of human \u03b3-secretase",
      "volume": "512",
      "author": "Lu",
      "year": "2014",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0390",
      "doi-asserted-by": "crossref",
      "first-page": "e03665",
      "DOI": "10.7554/eLife.03665",
      "article-title": "Beam-induced motion correction for sub-megadalton cryo-EM particles",
      "volume": "3",
      "author": "Scheres",
      "year": "2014",
      "journal-title": "Elife"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0395",
      "doi-asserted-by": "crossref",
      "first-page": "1240585",
      "DOI": "10.1126/science.1240585",
      "article-title": "Molecular architecture of a eukaryotic translational initiation complex",
      "volume": "342",
      "author": "Fern\u00e1ndez",
      "year": "2013",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0400",
      "doi-asserted-by": "crossref",
      "first-page": "9139",
      "DOI": "10.1073/pnas.1406335111",
      "article-title": "Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA-like structural element in the ribosomal decoding center",
      "volume": "111",
      "author": "Koh",
      "year": "2014",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0405",
      "doi-asserted-by": "crossref",
      "first-page": "823",
      "DOI": "10.1016/j.cell.2014.04.015",
      "article-title": "Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome",
      "volume": "157",
      "author": "Fern\u00e1ndez",
      "year": "2014",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0410",
      "doi-asserted-by": "crossref",
      "first-page": "75",
      "DOI": "10.1016/0304-3991(96)00045-9",
      "article-title": "Electron radiation damage to protein crystals of bacteriorhodopsin at different temperatures",
      "volume": "63",
      "author": "Stark",
      "year": "1996",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0415",
      "doi-asserted-by": "crossref",
      "first-page": "531",
      "DOI": "10.1016/j.jsb.2013.04.014",
      "article-title": "Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: reduction of electron-optical effects of charging",
      "volume": "183",
      "author": "Sader",
      "year": "2013",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0420",
      "doi-asserted-by": "crossref",
      "first-page": "649",
      "DOI": "10.1038/nmeth.2931",
      "article-title": "Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas",
      "volume": "11",
      "author": "Russo",
      "year": "2014",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0425",
      "doi-asserted-by": "crossref",
      "first-page": "42",
      "DOI": "10.1016/j.jsb.2013.11.002",
      "article-title": "Fabrication of carbon films with \u223c500nm holes for cryo-EM with a direct detector device",
      "volume": "185",
      "author": "Marr",
      "year": "2014",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0430",
      "doi-asserted-by": "crossref",
      "first-page": "106",
      "DOI": "10.1016/j.ultramic.2012.03.006",
      "article-title": "Paraxial charge compensator for electron cryomicroscopy",
      "volume": "116",
      "author": "Berriman",
      "year": "2012",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0435",
      "doi-asserted-by": "crossref",
      "first-page": "117",
      "DOI": "10.1016/j.jsb.2006.05.004",
      "article-title": "FREALIGN: high-resolution refinement of single particle structures",
      "volume": "157",
      "author": "Grigorieff",
      "year": "2007",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0440",
      "doi-asserted-by": "crossref",
      "first-page": "82",
      "DOI": "10.1006/jsbi.1999.4174",
      "article-title": "EMAN: semiautomated software for high-resolution single-particle reconstructions",
      "volume": "128",
      "author": "Ludtke",
      "year": "1999",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0445",
      "doi-asserted-by": "crossref",
      "first-page": "11",
      "DOI": "10.1016/j.jsb.2007.06.009",
      "article-title": "Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a Multi-Path Simulated Annealing optimization algorithm",
      "volume": "160",
      "author": "Liu",
      "year": "2007",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0450",
      "doi-asserted-by": "crossref",
      "first-page": "477",
      "DOI": "10.1128/JVI.02641-13",
      "article-title": "Near-atomic resolution cryo-electron microscopic structure of dengue serotype 4 virus",
      "volume": "88",
      "author": "Kostyuchenko",
      "year": "2014",
      "journal-title": "J. Virol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0455",
      "doi-asserted-by": "crossref",
      "first-page": "5504",
      "DOI": "10.1073/pnas.1300601110",
      "article-title": "Atomic structure of the 75MDa extremophile Sulfolobus turreted icosahedral virus determined by CryoEM and X-ray crystallography",
      "volume": "110",
      "author": "Veesler",
      "year": "2013",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0460",
      "doi-asserted-by": "crossref",
      "first-page": "17",
      "DOI": "10.1006/jsbi.1996.0004",
      "article-title": "A new generation of the IMAGIC image processing system",
      "volume": "116",
      "author": "Van Heel",
      "year": "1996",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.tibs.2014.10.005_bib0465",
      "doi-asserted-by": "crossref",
      "first-page": "515",
      "DOI": "10.1038/nature12890",
      "article-title": "Architecture of the large subunit of the mammalian mitochondrial ribosome",
      "volume": "505",
      "author": "Greber",
      "year": "2013",
      "journal-title": "Nature"
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