DOI: 10.1016/j.str.2012.06.010. Structure and 3D Arrangement of Endoplasmic Reticulum Membrane-Associated Ribosomes

Structure and 3D Arrangement of Endoplasmic Reticulum Membrane-Associated Ribosomes

10.1016/j.str.2012.06.010

Crossref journal-article

Elsevier BV

Structure (78)

Bibliography

Pfeffer, S., Brandt, F., Hrabe, T., Lang, S., Eibauer, M., Zimmermann, R., & FÃ¶rster, F. (2012). Structure and 3D Arrangement of Endoplasmic Reticulum Membrane-Associated Ribosomes. Structure, 20(9), 1508â1518.

Authors 7

Stefan Pfeffer (first)
Florian Brandt (additional)
Thomas Hrabe (additional)
Sven Lang (additional)
Matthias Eibauer (additional)
Richard Zimmermann (additional)
Friedrich Förster (additional)

References 60 Referenced 90

10.1073/pnas.1009999107 / Proc. Natl. Acad. Sci. USA / Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution by Armache (2010)
10.1073/pnas.1010005107 / Proc. Natl. Acad. Sci. USA / Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome by Armache (2010)
10.1016/j.sbi.2009.06.005 / Curr. Opin. Struct. Biol. / Membrane protein structure determination using cryo-electron tomography and 3D image averaging by Bartesaghi (2009)
10.1126/science.1178535 / Science / Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome by Becker (2009)
10.1016/S0092-8674(01)00541-4 / Cell / Architecture of the protein-conducting channel associated with the translating 80S ribosome by Beckmann (2001)
10.1126/science.1194294 / Science / Crystal structure of the eukaryotic ribosome by Ben-Shem (2010)
10.1126/science.1212642 / Science / The structure of the eukaryotic ribosome at 3.0 Å resolution by Ben-Shem (2011)
10.1038/nsmb998 / Nat. Struct. Mol. Biol. / ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane by Blau (2005)
10.1083/jcb.67.3.835 / J. Cell Biol. / Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma by Blobel (1975)
10.1083/jcb.67.3.852 / J. Cell Biol. / Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components by Blobel (1975)
10.1016/j.cell.2008.11.016 / Cell / The native 3D organization of bacterial polysomes by Brandt (2009)
10.1016/j.molcel.2010.08.003 / Mol. Cell / The three-dimensional organization of polyribosomes in intact human cells by Brandt (2010)
10.1038/nature00827 / Nature / Three-dimensional structure of the bacterial protein-translocation complex SecYEG by Breyton (2002)
10.1016/j.str.2008.01.007 / Structure / Structure of the mammalian 80S ribosome at 8.7 A resolution by Chandramouli (2008)
10.1016/j.bbamem.2010.08.016 / Biochim. Biophys. Acta / The Sec translocase by du Plessis (2011)
10.1093/emboj/cdf315 / EMBO J. / A novel type of co-chaperone mediates transmembrane recruitment of DnaK-like chaperones to ribosomes by Dudek (2002)
10.1038/nsmb1007 / Nat. Struct. Mol. Biol. / ERj1p has a basic role in protein biogenesis at the endoplasmic reticulum by Dudek (2005)
10.1073/pnas.83.3.581 / Proc. Natl. Acad. Sci. USA / Purification of microsomal signal peptidase as a complex by Evans (1986)
10.1016/j.ultramic.2006.02.004 / Ultramicroscopy / CTF determination and correction in electron cryotomography by Fernández (2006)
10.1016/S0091-679X(06)79029-X / Methods Cell Biol. / Structure determination in situ by averaging of tomograms by Förster (2007)
10.1073/pnas.0409178102 / Proc. Natl. Acad. Sci. USA / Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography by Förster (2005)
10.1016/j.jsb.2007.07.006 / J. Struct. Biol. / Classification of cryo-electron sub-tomograms using constrained correlation by Förster (2008)
10.1016/S0076-6879(10)83011-3 / Methods Enzymol. / Visual proteomics by Förster (2010)
10.1073/pnas.172520299 / Proc. Natl. Acad. Sci. USA / Identification of macromolecular complexes in cryoelectron tomograms of phantom cells by Frangakis (2002)
10.1038/nsmb.2026 / Nat. Struct. Mol. Biol. / Cryo-EM structure of the ribosome-SecYE complex in the membrane environment by Frauenfeld (2011)
10.1016/j.jsb.2006.06.010 / J. Struct. Biol. / Visualizing density maps with UCSF Chimera by Goddard (2007)
10.1016/0092-8674(93)90483-7 / Cell / Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane by Görlich (1993)
10.1038/357047a0 / Nature / A protein of the endoplasmic reticulum involved early in polypeptide translocation by Görlich (1992)
{'key': '10.1016/j.str.2012.06.010_bib29', 'first-page': '589', 'article-title': 'Evolution of the modular structure of rRNA', 'author': 'Gray', 'year': '1990'} / Evolution of the modular structure of rRNA by Gray (1990)
10.1111/j.1432-1033.1993.tb17933.x / Eur. J. Biochem. / A tetrameric complex of membrane proteins in the endoplasmic reticulum by Hartmann (1993)
10.1016/j.jsb.2011.12.003 / J. Struct. Biol. / PyTom: a python-based toolbox for localization of macromolecules in cryo-electron tomograms and subtomogram analysis by Hrabe (2012)
10.1093/glycob/cwj066 / Glycobiology / An evolving view of the eukaryotic oligosaccharyltransferase by Kelleher (2006)
10.1126/science.1211204 / Science / Crystal structure of the eukaryotic 60S ribosomal subunit in complex with initiation factor 6 by Klinge (2011)
10.1083/jcb.49.3.683 / J. Cell Biol. / Attachment of ribosomes to membranes during polysome formation in mouse sarcoma 180 cells by Lee (1971)
10.1146/annurev.biochem.73.011303.074112 / Annu. Rev. Biochem. / Structural studies by electron tomography: from cells to molecules by Lucić (2005)
10.1016/j.str.2008.05.003 / Structure / Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome by Ménétret (2008)
10.1038/nature04133 / Nature / Structure of the E. coli protein-conducting channel bound to a translating ribosome by Mitra (2005)
10.1091/mbc.e09-08-0730 / Mol. Biol. Cell / Evolutionary gain of function for the ER membrane protein Sec62 from yeast to humans by Müller (2010)
10.1016/j.jsb.2004.10.006 / J. Struct. Biol. / TOM software toolbox: acquisition and analysis for electron tomography by Nickell (2005)
10.1016/S0021-9258(18)53389-5 / J. Biol. Chem. / Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane by Nilsson (1993)
10.1016/j.jsb.2010.09.017 / J. Struct. Biol. / Exploring vitreous cryo-section-induced compression at the macromolecular level using electron cryo-tomography; 80S yeast ribosomes appear unaffected by Pierson (2011)
10.1074/jbc.M005294200 / J. Biol. Chem. / Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane by Potter (2000)
10.1074/jbc.M202559200 / J. Biol. Chem. / Endoplasmic reticulum-bound ribosomes reside in stable association with the translocon following termination of protein synthesis by Potter (2002)
10.1126/science.1198308 / Science / Crystal structure of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1 by Rabl (2011)
10.1073/pnas.1201333109 / Proc. Natl. Acad. Sci. USA / Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography by Rigort (2012)
10.1091/mbc.01-10-0514 / Mol. Biol. Cell / Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons by Rolls (2002)
10.1016/j.jmb.2006.11.052 / J. Mol. Biol. / Sec61alpha and TRAM are sequentially adjacent to a nascent viral membrane protein during its ER integration by Saurí (2007)
10.1038/346540a0 / Nature / Identification of a ribosome receptor in the rough endoplasmic reticulum by Savitz (1990)
10.1091/mbc.e06-05-0439 / Mol. Biol. Cell / Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane by Schaletzky (2006)
10.1016/j.cell.2010.11.007 / Cell / Mechanisms determining the morphology of the peripheral ER by Shibata (2010)
10.1083/jcb.200312079 / J. Cell Biol. / The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells by Snapp (2004)
10.1016/j.cell.2004.08.001 / Cell / Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor by Spahn (2004)
10.1091/mbc.e07-07-0677 / Mol. Biol. Cell / Divergent regulation of protein synthesis in the cytosol and endoplasmic reticulum compartments of mammalian cells by Stephens (2008)
{'key': '10.1016/j.str.2012.06.010_bib54', 'first-page': '4203', 'article-title': 'An rRNA variable region has an evolutionarily conserved essential role despite sequence divergence', 'volume': '14', 'author': 'Sweeney', 'year': '1994', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / An rRNA variable region has an evolutionarily conserved essential role despite sequence divergence by Sweeney (1994)
10.1073/pnas.97.13.7214 / Proc. Natl. Acad. Sci. USA / Homologs of the yeast Sec complex subunits Sec62p and Sec63p are abundant proteins in dog pancreas microsomes by Tyedmers (2000)
10.1093/nar/gkr1197 / Nucleic Acids Res. / Regulation of polysome assembly on the endoplasmic reticulum by a coiled-coil protein, p180 by Ueno (2012)
10.1038/nature02218 / Nature / X-ray structure of a protein-conducting channel by Van den Berg (2004)
10.1083/jcb.130.1.29 / J. Cell Biol. / Functional characterization of the 180-kD ribosome receptor in vivo by Wanker (1995)
10.1073/pnas.80.10.2809 / Proc. Natl. Acad. Sci. USA / M13 procoat and a pre-immunoglobulin share processing specificity but use different membrane receptor mechanisms by Watts (1983)
10.1002/pmic.200800722 / Proteomics / Analysis of the membrane proteome of canine pancreatic rough microsomes identifies a novel Hsp40, termed ERj7 by Zahedi (2009)

Dates

Type	When
Created	13 years, 1 month ago (July 19, 2012, 1:16 p.m.)
Deposited	3 years, 9 months ago (Nov. 2, 2021, 9:29 p.m.)
Indexed	20 minutes ago (Aug. 21, 2025, 4:34 a.m.)
Issued	12 years, 11 months ago (Sept. 1, 2012)
Published	12 years, 11 months ago (Sept. 1, 2012)
Published Print	12 years, 11 months ago (Sept. 1, 2012)

Funders 0

None

BibTeX

@article{Pfeffer_2012, title={Structure and 3D Arrangement of Endoplasmic Reticulum Membrane-Associated Ribosomes}, volume={20}, ISSN={0969-2126}, url={http://dx.doi.org/10.1016/j.str.2012.06.010}, DOI={10.1016/j.str.2012.06.010}, number={9}, journal={Structure}, publisher={Elsevier BV}, author={Pfeffer, Stefan and Brandt, Florian and Hrabe, Thomas and Lang, Sven and Eibauer, Matthias and Zimmermann, Richard and Förster, Friedrich}, year={2012}, month=sep, pages={1508–1518} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        21
      ]
    ],
    "date-time": "2025-08-21T08:34:54Z",
    "timestamp": 1755765294680
  },
  "reference-count": 60,
  "publisher": "Elsevier BV",
  "issue": "9",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2012,
            9,
            1
          ]
        ],
        "date-time": "2012-09-01T00:00:00Z",
        "timestamp": 1346457600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    },
    {
      "start": {
        "date-parts": [
          [
            2013,
            9,
            5
          ]
        ],
        "date-time": "2013-09-05T00:00:00Z",
        "timestamp": 1378339200000
      },
      "content-version": "vor",
      "delay-in-days": 369,
      "URL": "http://www.elsevier.com/open-access/userlicense/1.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2012,
        9
      ]
    ]
  },
  "DOI": "10.1016/j.str.2012.06.010",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2012,
        7,
        19
      ]
    ],
    "date-time": "2012-07-19T17:16:17Z",
    "timestamp": 1342718177000
  },
  "page": "1508-1518",
  "source": "Crossref",
  "is-referenced-by-count": 90,
  "title": "Structure and 3D Arrangement of Endoplasmic Reticulum Membrane-Associated Ribosomes",
  "prefix": "10.1016",
  "volume": "20",
  "author": [
    {
      "given": "Stefan",
      "family": "Pfeffer",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Florian",
      "family": "Brandt",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Thomas",
      "family": "Hrabe",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Sven",
      "family": "Lang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Matthias",
      "family": "Eibauer",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Richard",
      "family": "Zimmermann",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Friedrich",
      "family": "F\u00f6rster",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/j.str.2012.06.010_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "19748",
      "DOI": "10.1073/pnas.1009999107",
      "article-title": "Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution",
      "volume": "107",
      "author": "Armache",
      "year": "2010",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "19754",
      "DOI": "10.1073/pnas.1010005107",
      "article-title": "Localization of eukaryote-specific ribosomal proteins in a 5.5-\u00c5 cryo-EM map of the 80S eukaryotic ribosome",
      "volume": "107",
      "author": "Armache",
      "year": "2010",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "402",
      "DOI": "10.1016/j.sbi.2009.06.005",
      "article-title": "Membrane protein structure determination using cryo-electron tomography and 3D image averaging",
      "volume": "19",
      "author": "Bartesaghi",
      "year": "2009",
      "journal-title": "Curr. Opin. Struct. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "1369",
      "DOI": "10.1126/science.1178535",
      "article-title": "Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome",
      "volume": "326",
      "author": "Becker",
      "year": "2009",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "361",
      "DOI": "10.1016/S0092-8674(01)00541-4",
      "article-title": "Architecture of the protein-conducting channel associated with the translating 80S ribosome",
      "volume": "107",
      "author": "Beckmann",
      "year": "2001",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "1203",
      "DOI": "10.1126/science.1194294",
      "article-title": "Crystal structure of the eukaryotic ribosome",
      "volume": "330",
      "author": "Ben-Shem",
      "year": "2010",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "1524",
      "DOI": "10.1126/science.1212642",
      "article-title": "The structure of the eukaryotic ribosome at 3.0\u00a0\u00c5 resolution",
      "volume": "334",
      "author": "Ben-Shem",
      "year": "2011",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "1015",
      "DOI": "10.1038/nsmb998",
      "article-title": "ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane",
      "volume": "12",
      "author": "Blau",
      "year": "2005",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "835",
      "DOI": "10.1083/jcb.67.3.835",
      "article-title": "Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma",
      "volume": "67",
      "author": "Blobel",
      "year": "1975",
      "journal-title": "J.\u00a0Cell Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "852",
      "DOI": "10.1083/jcb.67.3.852",
      "article-title": "Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components",
      "volume": "67",
      "author": "Blobel",
      "year": "1975",
      "journal-title": "J.\u00a0Cell Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "261",
      "DOI": "10.1016/j.cell.2008.11.016",
      "article-title": "The native 3D organization of bacterial polysomes",
      "volume": "136",
      "author": "Brandt",
      "year": "2009",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "560",
      "DOI": "10.1016/j.molcel.2010.08.003",
      "article-title": "The three-dimensional organization of polyribosomes in intact human cells",
      "volume": "39",
      "author": "Brandt",
      "year": "2010",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "662",
      "DOI": "10.1038/nature00827",
      "article-title": "Three-dimensional structure of the bacterial protein-translocation complex SecYEG",
      "volume": "418",
      "author": "Breyton",
      "year": "2002",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "535",
      "DOI": "10.1016/j.str.2008.01.007",
      "article-title": "Structure of the mammalian 80S ribosome at 8.7 A resolution",
      "volume": "16",
      "author": "Chandramouli",
      "year": "2008",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "851",
      "DOI": "10.1016/j.bbamem.2010.08.016",
      "article-title": "The Sec translocase",
      "volume": "1808",
      "author": "du Plessis",
      "year": "2011",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "2958",
      "DOI": "10.1093/emboj/cdf315",
      "article-title": "A novel type of co-chaperone mediates transmembrane recruitment of DnaK-like chaperones to ribosomes",
      "volume": "21",
      "author": "Dudek",
      "year": "2002",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "1008",
      "DOI": "10.1038/nsmb1007",
      "article-title": "ERj1p has a basic role in protein biogenesis at the endoplasmic reticulum",
      "volume": "12",
      "author": "Dudek",
      "year": "2005",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "581",
      "DOI": "10.1073/pnas.83.3.581",
      "article-title": "Purification of microsomal signal peptidase as a complex",
      "volume": "83",
      "author": "Evans",
      "year": "1986",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib19",
      "doi-asserted-by": "crossref",
      "first-page": "587",
      "DOI": "10.1016/j.ultramic.2006.02.004",
      "article-title": "CTF determination and correction in electron cryotomography",
      "volume": "106",
      "author": "Fern\u00e1ndez",
      "year": "2006",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib20",
      "doi-asserted-by": "crossref",
      "first-page": "741",
      "DOI": "10.1016/S0091-679X(06)79029-X",
      "article-title": "Structure determination in\u00a0situ by averaging of tomograms",
      "volume": "79",
      "author": "F\u00f6rster",
      "year": "2007",
      "journal-title": "Methods Cell Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "4729",
      "DOI": "10.1073/pnas.0409178102",
      "article-title": "Retrovirus envelope protein complex structure in\u00a0situ studied by cryo-electron tomography",
      "volume": "102",
      "author": "F\u00f6rster",
      "year": "2005",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "276",
      "DOI": "10.1016/j.jsb.2007.07.006",
      "article-title": "Classification of cryo-electron sub-tomograms using constrained correlation",
      "volume": "161",
      "author": "F\u00f6rster",
      "year": "2008",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "215",
      "DOI": "10.1016/S0076-6879(10)83011-3",
      "article-title": "Visual proteomics",
      "volume": "483",
      "author": "F\u00f6rster",
      "year": "2010",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "14153",
      "DOI": "10.1073/pnas.172520299",
      "article-title": "Identification of macromolecular complexes in cryoelectron tomograms of phantom cells",
      "volume": "99",
      "author": "Frangakis",
      "year": "2002",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "614",
      "DOI": "10.1038/nsmb.2026",
      "article-title": "Cryo-EM structure of the ribosome-SecYE complex in the membrane environment",
      "volume": "18",
      "author": "Frauenfeld",
      "year": "2011",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "281",
      "DOI": "10.1016/j.jsb.2006.06.010",
      "article-title": "Visualizing density maps with UCSF Chimera",
      "volume": "157",
      "author": "Goddard",
      "year": "2007",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "615",
      "DOI": "10.1016/0092-8674(93)90483-7",
      "article-title": "Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane",
      "volume": "75",
      "author": "G\u00f6rlich",
      "year": "1993",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "47",
      "DOI": "10.1038/357047a0",
      "article-title": "A protein of the\u00a0endoplasmic reticulum involved early in polypeptide translocation",
      "volume": "357",
      "author": "G\u00f6rlich",
      "year": "1992",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib29",
      "first-page": "589",
      "article-title": "Evolution of the modular structure of rRNA",
      "author": "Gray",
      "year": "1990"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "375",
      "DOI": "10.1111/j.1432-1033.1993.tb17933.x",
      "article-title": "A tetrameric complex of membrane proteins in the endoplasmic reticulum",
      "volume": "214",
      "author": "Hartmann",
      "year": "1993",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "177",
      "DOI": "10.1016/j.jsb.2011.12.003",
      "article-title": "PyTom: a python-based toolbox for localization of macromolecules in\u00a0cryo-electron tomograms and subtomogram analysis",
      "volume": "178",
      "author": "Hrabe",
      "year": "2012",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib32",
      "doi-asserted-by": "crossref",
      "first-page": "47R",
      "DOI": "10.1093/glycob/cwj066",
      "article-title": "An evolving view of the eukaryotic oligosaccharyltransferase",
      "volume": "16",
      "author": "Kelleher",
      "year": "2006",
      "journal-title": "Glycobiology"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "941",
      "DOI": "10.1126/science.1211204",
      "article-title": "Crystal structure of the eukaryotic 60S ribosomal subunit in complex with initiation factor 6",
      "volume": "334",
      "author": "Klinge",
      "year": "2011",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "683",
      "DOI": "10.1083/jcb.49.3.683",
      "article-title": "Attachment of ribosomes to membranes during polysome formation in mouse sarcoma 180 cells",
      "volume": "49",
      "author": "Lee",
      "year": "1971",
      "journal-title": "J.\u00a0Cell Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "833",
      "DOI": "10.1146/annurev.biochem.73.011303.074112",
      "article-title": "Structural studies by electron tomography: from cells to molecules",
      "volume": "74",
      "author": "Luci\u0107",
      "year": "2005",
      "journal-title": "Annu. Rev. Biochem."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib36",
      "doi-asserted-by": "crossref",
      "first-page": "1126",
      "DOI": "10.1016/j.str.2008.05.003",
      "article-title": "Single copies of Sec61 and TRAP associate with\u00a0a nontranslating mammalian ribosome",
      "volume": "16",
      "author": "M\u00e9n\u00e9tret",
      "year": "2008",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib37",
      "doi-asserted-by": "crossref",
      "first-page": "318",
      "DOI": "10.1038/nature04133",
      "article-title": "Structure of the E. coli protein-conducting channel bound to a translating ribosome",
      "volume": "438",
      "author": "Mitra",
      "year": "2005",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib38",
      "doi-asserted-by": "crossref",
      "first-page": "691",
      "DOI": "10.1091/mbc.e09-08-0730",
      "article-title": "Evolutionary gain of function for the ER membrane protein Sec62 from yeast to humans",
      "volume": "21",
      "author": "M\u00fcller",
      "year": "2010",
      "journal-title": "Mol. Biol. Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib39",
      "doi-asserted-by": "crossref",
      "first-page": "227",
      "DOI": "10.1016/j.jsb.2004.10.006",
      "article-title": "TOM software toolbox: acquisition and analysis for electron tomography",
      "volume": "149",
      "author": "Nickell",
      "year": "2005",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib40",
      "doi-asserted-by": "crossref",
      "first-page": "5798",
      "DOI": "10.1016/S0021-9258(18)53389-5",
      "article-title": "Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane",
      "volume": "268",
      "author": "Nilsson",
      "year": "1993",
      "journal-title": "J.\u00a0Biol. Chem."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib41",
      "doi-asserted-by": "crossref",
      "first-page": "345",
      "DOI": "10.1016/j.jsb.2010.09.017",
      "article-title": "Exploring vitreous cryo-section-induced compression at the macromolecular level using electron cryo-tomography; 80S yeast ribosomes appear unaffected",
      "volume": "173",
      "author": "Pierson",
      "year": "2011",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib42",
      "doi-asserted-by": "crossref",
      "first-page": "33828",
      "DOI": "10.1074/jbc.M005294200",
      "article-title": "Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane",
      "volume": "275",
      "author": "Potter",
      "year": "2000",
      "journal-title": "J.\u00a0Biol. Chem."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib43",
      "doi-asserted-by": "crossref",
      "first-page": "23314",
      "DOI": "10.1074/jbc.M202559200",
      "article-title": "Endoplasmic reticulum-bound ribosomes reside in stable association with the translocon following termination of protein synthesis",
      "volume": "277",
      "author": "Potter",
      "year": "2002",
      "journal-title": "J.\u00a0Biol. Chem."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib44",
      "doi-asserted-by": "crossref",
      "first-page": "730",
      "DOI": "10.1126/science.1198308",
      "article-title": "Crystal structure of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1",
      "volume": "331",
      "author": "Rabl",
      "year": "2011",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib45",
      "doi-asserted-by": "crossref",
      "first-page": "4449",
      "DOI": "10.1073/pnas.1201333109",
      "article-title": "Focused ion beam micromachining of eukaryotic cells\u00a0for cryoelectron tomography",
      "volume": "109",
      "author": "Rigort",
      "year": "2012",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib46",
      "doi-asserted-by": "crossref",
      "first-page": "1778",
      "DOI": "10.1091/mbc.01-10-0514",
      "article-title": "Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons",
      "volume": "13",
      "author": "Rolls",
      "year": "2002",
      "journal-title": "Mol. Biol. Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib47",
      "doi-asserted-by": "crossref",
      "first-page": "366",
      "DOI": "10.1016/j.jmb.2006.11.052",
      "article-title": "Sec61alpha and TRAM are sequentially adjacent to a nascent viral membrane protein during its ER integration",
      "volume": "366",
      "author": "Saur\u00ed",
      "year": "2007",
      "journal-title": "J.\u00a0Mol. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib48",
      "doi-asserted-by": "crossref",
      "first-page": "540",
      "DOI": "10.1038/346540a0",
      "article-title": "Identification of a ribosome receptor in the rough endoplasmic reticulum",
      "volume": "346",
      "author": "Savitz",
      "year": "1990",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib49",
      "doi-asserted-by": "crossref",
      "first-page": "3860",
      "DOI": "10.1091/mbc.e06-05-0439",
      "article-title": "Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane",
      "volume": "17",
      "author": "Schaletzky",
      "year": "2006",
      "journal-title": "Mol. Biol. Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib50",
      "doi-asserted-by": "crossref",
      "first-page": "774",
      "DOI": "10.1016/j.cell.2010.11.007",
      "article-title": "Mechanisms determining the morphology of the peripheral ER",
      "volume": "143",
      "author": "Shibata",
      "year": "2010",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib51",
      "doi-asserted-by": "crossref",
      "first-page": "997",
      "DOI": "10.1083/jcb.200312079",
      "article-title": "The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells",
      "volume": "164",
      "author": "Snapp",
      "year": "2004",
      "journal-title": "J.\u00a0Cell Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib52",
      "doi-asserted-by": "crossref",
      "first-page": "465",
      "DOI": "10.1016/j.cell.2004.08.001",
      "article-title": "Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor",
      "volume": "118",
      "author": "Spahn",
      "year": "2004",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib53",
      "doi-asserted-by": "crossref",
      "first-page": "623",
      "DOI": "10.1091/mbc.e07-07-0677",
      "article-title": "Divergent regulation of protein synthesis in the cytosol and endoplasmic reticulum compartments of mammalian cells",
      "volume": "19",
      "author": "Stephens",
      "year": "2008",
      "journal-title": "Mol. Biol. Cell"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib54",
      "first-page": "4203",
      "article-title": "An rRNA variable region has an evolutionarily conserved essential role despite sequence divergence",
      "volume": "14",
      "author": "Sweeney",
      "year": "1994",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib55",
      "doi-asserted-by": "crossref",
      "first-page": "7214",
      "DOI": "10.1073/pnas.97.13.7214",
      "article-title": "Homologs of the yeast Sec complex subunits Sec62p and Sec63p are abundant proteins in dog pancreas microsomes",
      "volume": "97",
      "author": "Tyedmers",
      "year": "2000",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib56",
      "doi-asserted-by": "crossref",
      "first-page": "3006",
      "DOI": "10.1093/nar/gkr1197",
      "article-title": "Regulation of polysome assembly on the endoplasmic reticulum by a coiled-coil protein, p180",
      "volume": "40",
      "author": "Ueno",
      "year": "2012",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib57",
      "doi-asserted-by": "crossref",
      "first-page": "36",
      "DOI": "10.1038/nature02218",
      "article-title": "X-ray structure of a protein-conducting channel",
      "volume": "427",
      "author": "Van den Berg",
      "year": "2004",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib58",
      "doi-asserted-by": "crossref",
      "first-page": "29",
      "DOI": "10.1083/jcb.130.1.29",
      "article-title": "Functional characterization of the 180-kD ribosome receptor in\u00a0vivo",
      "volume": "130",
      "author": "Wanker",
      "year": "1995",
      "journal-title": "J.\u00a0Cell Biol."
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib59",
      "doi-asserted-by": "crossref",
      "first-page": "2809",
      "DOI": "10.1073/pnas.80.10.2809",
      "article-title": "M13 procoat and a pre-immunoglobulin share processing specificity but use different membrane receptor mechanisms",
      "volume": "80",
      "author": "Watts",
      "year": "1983",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.str.2012.06.010_bib60",
      "doi-asserted-by": "crossref",
      "first-page": "3463",
      "DOI": "10.1002/pmic.200800722",
      "article-title": "Analysis of the membrane proteome of canine pancreatic rough microsomes identifies a novel\u00a0Hsp40, termed ERj7",
      "volume": "9",
      "author": "Zahedi",
      "year": "2009",
      "journal-title": "Proteomics"
    }
  ],
  "container-title": "Structure",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0969212612002328?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0969212612002328?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        3
      ]
    ],
    "date-time": "2021-11-03T01:29:50Z",
    "timestamp": 1635902990000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S0969212612002328"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2012,
        9
      ]
    ]
  },
  "references-count": 60,
  "journal-issue": {
    "issue": "9",
    "published-print": {
      "date-parts": [
        [
          2012,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "S0969212612002328"
  ],
  "URL": "http://dx.doi.org/10.1016/j.str.2012.06.010",
  "relation": {},
  "ISSN": [
    "0969-2126"
  ],
  "subject": [],
  "container-title-short": "Structure",
  "published": {
    "date-parts": [
      [
        2012,
        9
      ]
    ]
  }
}