DOI: 10.1016/j.str.2005.06.008. Crystallographic Refinement by Knowledge-Based Exploration of Complex Energy Landscapes

Crystallographic Refinement by Knowledge-Based Exploration of Complex Energy Landscapes

10.1016/j.str.2005.06.008

Crossref journal-article

Elsevier BV

Structure (78)

Authors 4

Mark A. DePristo (first)
Paul I.W. de Bakker (additional)
Russell J.K. Johnson (additional)
Tom L. Blundell (additional)

References 36 Referenced 33

{'key': '10.1016/j.str.2005.06.008_bib1', 'series-title': 'Protein Crystallography', 'author': 'Blundell', 'year': '1976'} / Protein Crystallography by Blundell (1976)
10.1038/326347a0 / Nature / Knowledge-based prediction of protein structures and the design of novel molecules by Blundell (1987)
10.1111/j.1432-1033.1988.tb13917.x / Eur. J. Biochem. / 18th Sir Hans Krebs lecture. Knowledge-based protein modelling and design by Blundell (1988)
10.1038/355472a0 / Nature / The free R value: a novel statistical quantity for assessing the accuracy of crystal structures by Brunger (1992)
10.1126/science.235.4787.458 / Science / Crystallographic R factor refinement by molecular dynamics by Brunger (1987)
10.1107/S0907444998003254 / Acta Crystallogr. D Biol. Crystallogr. / Crystallography & NMR system: a new software suite for macromolecular structure determination by Brunger (1998)
{'key': '10.1016/j.str.2005.06.008_bib7', 'series-title': 'Ab initio Sampling of Polypeptide Conformations and the Prediction of Protein Structure', 'author': 'de Bakker', 'year': '2003'} / Ab initio Sampling of Polypeptide Conformations and the Prediction of Protein Structure by de Bakker (2003)
10.1002/prot.10235 / Proteins / Ab initio construction of polypeptide fragments: accuracy of loop decoy discrimination by an all-atom statistical potential and the AMBER force field with the Generalized Born solvation model by de Bakker (2003)
DeLano, W.L. (2002). The PyMOL Molecular Graphics System (http://www.pymol.org/).
10.1002/prot.10285 / Proteins / Ab initio construction of polypeptide fragments: efficient generation of accurate, representative ensembles by DePristo (2003)
10.1110/ps.0386903 / Protein Sci. / Discrete restraint-based protein modeling and the Cα-trace problem by DePristo (2003)
10.1016/j.str.2004.02.031 / Structure / Heterogeneity and inaccuracy in protein structures solved by x-ray crystallography by DePristo (2004)
{'key': '10.1016/j.str.2005.06.008_bib13', 'series-title': 'Principles of Protein X-Ray Crystallography', 'author': 'Drenth', 'year': '1999'} / Principles of Protein X-Ray Crystallography by Drenth (1999)
10.1107/S0108767391001071 / Acta Crystallogr. A / Accurate bond and angle parameters for X-ray protein structure refinement by Engh (1991)
10.1126/science.299.5613.1663a / Science / Structural genomics: current progress by Gerstein (2003)
10.1038/nsmb0104-12 / Nat. Struct. Mol. Biol. / Whither structural biology? by Harrison (2004)
10.1016/0076-6879(85)15021-4 / In Methods in Enzymology / Stereochemically restrained refinement of macromolecular structures by Hendrickson (1985)
10.1107/S0108767392006044 / Acta Crystallogr. A / Model bias in macromolecular crystal structures by Hodel (1992)
Johnson, R.J.K., Christodoulou, J., Dumoulin, M., Caddy, G.L., Alocer, M.J., Murtagh, G.J., Kumita, J.R., Larsson, G., Robinson, C.V., Archer, D.B., Luisi, B., and Dobson, C.M. (2005). Rationalising Lysozyme Amyloidosis: Insights from the Structure and Solution Dynamics of T70N Lysozyme. J. Mol. Biol., in press (10.1016/j.jmb.2005.07.040)
10.1002/j.1460-2075.1986.tb04287.x / EMBO J. / Using known substructures in protein model building and crystallography by Jones (1986)
10.1107/S0108767390010224 / Acta Crystallogr. A / Improved methods for building protein models in electron density maps and the location of errors in these models by Jones (1991)
10.1016/S0969-2126(02)00743-8 / Structure / Homo crystallographicus–quo vadis? by Kleywegt (2002)
10.1107/S0907444996001783 / Acta Crystallogr. D Biol. Crystallogr. / Efficient rebuilding of protein structures by Kleywegt (1996)
10.1074/jbc.M204371200 / J. Biol. Chem. / X-ray structure and ligand binding study of a moth chemosensory protein by Lartigue (2002)
10.1002/1097-0134(20000815)40:3<389::AID-PROT50>3.0.CO;2-2 / Proteins / The penultimate rotamer library by Lovell (2000)
10.1002/prot.10286 / Proteins / Structure validation by Cα geometry: ϕ, ψ, and Cβ deviation by Lovell (2003)
10.1107/S0907444996012255 / Acta Crystallogr. D Biol. Crystallogr. / Refinement of macromolecular structures by the maximum-likelihood method by Murshudov (1997)
10.1107/S0108767393007597 / Acta Crystallogr. A / AMoRe: an automated package for molecular replacement by Navaza (1994)
10.1016/S0076-6879(97)76066-X / Methods Enzymol. / Processing of X-ray diffraction data collected in oscillation mode by Otwinowski (1997)
10.1038/8263 / Nat. Struct. Biol. / Automated protein model building combined with iterative structure refinement by Perrakis (1999)
10.1107/S0907444901014007 / Acta Crystallogr. D Biol. Crystallogr. / ARP/wARP and molecular replacement by Perrakis (2001)
10.1006/jmbi.1993.1626 / J. Mol. Biol. / Comparative protein modelling by satisfaction of spatial restraints by Sali (1993)
10.1093/protein/gzg143 / Protein Eng. / Advantages of fine-grained side chain conformer libraries for protein modelling by Shetty (2003)
10.1107/S090744490402356X / Acta Crystallogr. D Biol. Crystallogr. / Introduction to macromolecular refinement by Tronrud (2004)
10.1107/S0567740873003675 / Acta Crystallogr. B / Refinement of the model of a protein: rubredoxin at 1.5 Å resolution by Watenpaugh (1973)
10.1073/pnas.96.1.103 / Proc. Natl. Acad. Sci. USA / Disordered water within a hydrophobic protein cavity visualized by X-ray crystallography by Yu (1999)

Dates

Type	When
Created	19 years, 11 months ago (Sept. 14, 2005, 6:53 p.m.)
Deposited	6 years, 5 months ago (March 22, 2019, 12:37 p.m.)
Indexed	1 year ago (Aug. 3, 2024, 2:14 p.m.)
Issued	19 years, 11 months ago (Sept. 1, 2005)
Published	19 years, 11 months ago (Sept. 1, 2005)
Published Print	19 years, 11 months ago (Sept. 1, 2005)

Funders 0

None

BibTeX

@article{DePristo_2005, title={Crystallographic Refinement by Knowledge-Based Exploration of Complex Energy Landscapes}, volume={13}, ISSN={0969-2126}, url={http://dx.doi.org/10.1016/j.str.2005.06.008}, DOI={10.1016/j.str.2005.06.008}, number={9}, journal={Structure}, publisher={Elsevier BV}, author={DePristo, Mark A. and de Bakker, Paul I.W. and Johnson, Russell J.K. and Blundell, Tom L.}, year={2005}, month=sep, pages={1311–1319} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        8,
        3
      ]
    ],
    "date-time": "2024-08-03T18:14:04Z",
    "timestamp": 1722708844334
  },
  "reference-count": 36,
  "publisher": "Elsevier BV",
  "issue": "9",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            9,
            1
          ]
        ],
        "date-time": "2005-09-01T00:00:00Z",
        "timestamp": 1125532800000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    },
    {
      "start": {
        "date-parts": [
          [
            2013,
            7,
            17
          ]
        ],
        "date-time": "2013-07-17T00:00:00Z",
        "timestamp": 1374019200000
      },
      "content-version": "vor",
      "delay-in-days": 2876,
      "URL": "https://www.elsevier.com/open-access/userlicense/1.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2005,
        9
      ]
    ]
  },
  "DOI": "10.1016/j.str.2005.06.008",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        9,
        14
      ]
    ],
    "date-time": "2005-09-14T22:53:42Z",
    "timestamp": 1126738422000
  },
  "page": "1311-1319",
  "source": "Crossref",
  "is-referenced-by-count": 33,
  "title": "Crystallographic Refinement by Knowledge-Based Exploration of Complex Energy Landscapes",
  "prefix": "10.1016",
  "volume": "13",
  "author": [
    {
      "given": "Mark A.",
      "family": "DePristo",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Paul I.W.",
      "family": "de Bakker",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Russell J.K.",
      "family": "Johnson",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Tom L.",
      "family": "Blundell",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/j.str.2005.06.008_bib1",
      "series-title": "Protein Crystallography",
      "author": "Blundell",
      "year": "1976"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "347",
      "DOI": "10.1038/326347a0",
      "article-title": "Knowledge-based prediction of protein structures and the design of novel molecules",
      "volume": "326",
      "author": "Blundell",
      "year": "1987",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "513",
      "DOI": "10.1111/j.1432-1033.1988.tb13917.x",
      "article-title": "18th Sir Hans Krebs lecture. Knowledge-based protein modelling and design",
      "volume": "172",
      "author": "Blundell",
      "year": "1988",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "472",
      "DOI": "10.1038/355472a0",
      "article-title": "The free R value: a novel statistical quantity for assessing the accuracy of crystal structures",
      "volume": "355",
      "author": "Brunger",
      "year": "1992",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "458",
      "DOI": "10.1126/science.235.4787.458",
      "article-title": "Crystallographic R factor refinement by molecular dynamics",
      "volume": "235",
      "author": "Brunger",
      "year": "1987",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "905",
      "DOI": "10.1107/S0907444998003254",
      "article-title": "Crystallography & NMR system: a new software suite for macromolecular structure determination",
      "volume": "54",
      "author": "Brunger",
      "year": "1998",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib7",
      "series-title": "Ab initio Sampling of Polypeptide Conformations and the Prediction of Protein Structure",
      "author": "de Bakker",
      "year": "2003"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "21",
      "DOI": "10.1002/prot.10235",
      "article-title": "Ab initio construction of polypeptide fragments: accuracy of loop decoy discrimination by an all-atom statistical potential and the AMBER force field with the Generalized Born solvation model",
      "volume": "51",
      "author": "de Bakker",
      "year": "2003",
      "journal-title": "Proteins"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib9",
      "unstructured": "DeLano, W.L. (2002). The PyMOL Molecular Graphics System (http://www.pymol.org/)."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "41",
      "DOI": "10.1002/prot.10285",
      "article-title": "Ab initio construction of polypeptide fragments: efficient generation of accurate, representative ensembles",
      "volume": "51",
      "author": "DePristo",
      "year": "2003",
      "journal-title": "Proteins"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "2032",
      "DOI": "10.1110/ps.0386903",
      "article-title": "Discrete restraint-based protein modeling and the C\u03b1-trace problem",
      "volume": "12",
      "author": "DePristo",
      "year": "2003",
      "journal-title": "Protein Sci."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "831",
      "DOI": "10.1016/j.str.2004.02.031",
      "article-title": "Heterogeneity and inaccuracy in protein structures solved by x-ray crystallography",
      "volume": "12",
      "author": "DePristo",
      "year": "2004",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib13",
      "series-title": "Principles of Protein X-Ray Crystallography",
      "author": "Drenth",
      "year": "1999"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "392",
      "DOI": "10.1107/S0108767391001071",
      "article-title": "Accurate bond and angle parameters for X-ray protein structure refinement",
      "volume": "47",
      "author": "Engh",
      "year": "1991",
      "journal-title": "Acta Crystallogr. A"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "1663",
      "DOI": "10.1126/science.299.5613.1663a",
      "article-title": "Structural genomics: current progress",
      "volume": "299",
      "author": "Gerstein",
      "year": "2003",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "12",
      "DOI": "10.1038/nsmb0104-12",
      "article-title": "Whither structural biology?",
      "volume": "11",
      "author": "Harrison",
      "year": "2004",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib17",
      "series-title": "In Methods in Enzymology",
      "doi-asserted-by": "crossref",
      "first-page": "252",
      "DOI": "10.1016/0076-6879(85)15021-4",
      "article-title": "Stereochemically restrained refinement of macromolecular structures",
      "author": "Hendrickson",
      "year": "1985"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "851",
      "DOI": "10.1107/S0108767392006044",
      "article-title": "Model bias in macromolecular crystal structures",
      "volume": "48",
      "author": "Hodel",
      "year": "1992",
      "journal-title": "Acta Crystallogr. A"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib19",
      "doi-asserted-by": "crossref",
      "unstructured": "Johnson, R.J.K., Christodoulou, J., Dumoulin, M., Caddy, G.L., Alocer, M.J., Murtagh, G.J., Kumita, J.R., Larsson, G., Robinson, C.V., Archer, D.B., Luisi, B., and Dobson, C.M. (2005). Rationalising Lysozyme Amyloidosis: Insights from the Structure and Solution Dynamics of T70N Lysozyme. J. Mol. Biol., in press",
      "DOI": "10.1016/j.jmb.2005.07.040"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib20",
      "doi-asserted-by": "crossref",
      "first-page": "819",
      "DOI": "10.1002/j.1460-2075.1986.tb04287.x",
      "article-title": "Using known substructures in protein model building and crystallography",
      "volume": "5",
      "author": "Jones",
      "year": "1986",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "110",
      "DOI": "10.1107/S0108767390010224",
      "article-title": "Improved methods for building protein models in electron density maps and the location of errors in these models",
      "volume": "47",
      "author": "Jones",
      "year": "1991",
      "journal-title": "Acta Crystallogr. A"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "465",
      "DOI": "10.1016/S0969-2126(02)00743-8",
      "article-title": "Homo crystallographicus\u2013quo vadis?",
      "volume": "10",
      "author": "Kleywegt",
      "year": "2002",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "829",
      "DOI": "10.1107/S0907444996001783",
      "article-title": "Efficient rebuilding of protein structures",
      "volume": "52",
      "author": "Kleywegt",
      "year": "1996",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "32094",
      "DOI": "10.1074/jbc.M204371200",
      "article-title": "X-ray structure and ligand binding study of a moth chemosensory protein",
      "volume": "277",
      "author": "Lartigue",
      "year": "2002",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "389",
      "DOI": "10.1002/1097-0134(20000815)40:3<389::AID-PROT50>3.0.CO;2-2",
      "article-title": "The penultimate rotamer library",
      "volume": "40",
      "author": "Lovell",
      "year": "2000",
      "journal-title": "Proteins"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "437",
      "DOI": "10.1002/prot.10286",
      "article-title": "Structure validation by C\u03b1 geometry: \u03d5, \u03c8, and C\u03b2 deviation",
      "volume": "50",
      "author": "Lovell",
      "year": "2003",
      "journal-title": "Proteins"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "240",
      "DOI": "10.1107/S0907444996012255",
      "article-title": "Refinement of macromolecular structures by the maximum-likelihood method",
      "volume": "53",
      "author": "Murshudov",
      "year": "1997",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "157",
      "DOI": "10.1107/S0108767393007597",
      "article-title": "AMoRe: an automated package for molecular replacement",
      "volume": "50",
      "author": "Navaza",
      "year": "1994",
      "journal-title": "Acta Crystallogr. A"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib29",
      "doi-asserted-by": "crossref",
      "first-page": "307",
      "DOI": "10.1016/S0076-6879(97)76066-X",
      "article-title": "Processing of X-ray diffraction data collected in oscillation mode",
      "volume": "276",
      "author": "Otwinowski",
      "year": "1997",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "458",
      "DOI": "10.1038/8263",
      "article-title": "Automated protein model building combined with iterative structure refinement",
      "volume": "6",
      "author": "Perrakis",
      "year": "1999",
      "journal-title": "Nat. Struct. Biol."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "1445",
      "DOI": "10.1107/S0907444901014007",
      "article-title": "ARP/wARP and molecular replacement",
      "volume": "57",
      "author": "Perrakis",
      "year": "2001",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib32",
      "doi-asserted-by": "crossref",
      "first-page": "779",
      "DOI": "10.1006/jmbi.1993.1626",
      "article-title": "Comparative protein modelling by satisfaction of spatial restraints",
      "volume": "234",
      "author": "Sali",
      "year": "1993",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "963",
      "DOI": "10.1093/protein/gzg143",
      "article-title": "Advantages of fine-grained side chain conformer libraries for protein modelling",
      "volume": "16",
      "author": "Shetty",
      "year": "2003",
      "journal-title": "Protein Eng."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "2156",
      "DOI": "10.1107/S090744490402356X",
      "article-title": "Introduction to macromolecular refinement",
      "volume": "60",
      "author": "Tronrud",
      "year": "2004",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "943",
      "DOI": "10.1107/S0567740873003675",
      "article-title": "Refinement of the model of a protein: rubredoxin at 1.5 \u00c5 resolution",
      "volume": "29",
      "author": "Watenpaugh",
      "year": "1973",
      "journal-title": "Acta Crystallogr. B"
    },
    {
      "key": "10.1016/j.str.2005.06.008_bib36",
      "doi-asserted-by": "crossref",
      "first-page": "103",
      "DOI": "10.1073/pnas.96.1.103",
      "article-title": "Disordered water within a hydrophobic protein cavity visualized by X-ray crystallography",
      "volume": "96",
      "author": "Yu",
      "year": "1999",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    }
  ],
  "container-title": "Structure",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0969212605002406?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0969212605002406?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2019,
        3,
        22
      ]
    ],
    "date-time": "2019-03-22T16:37:37Z",
    "timestamp": 1553272657000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S0969212605002406"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2005,
        9
      ]
    ]
  },
  "references-count": 36,
  "journal-issue": {
    "issue": "9",
    "published-print": {
      "date-parts": [
        [
          2005,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "S0969212605002406"
  ],
  "URL": "http://dx.doi.org/10.1016/j.str.2005.06.008",
  "relation": {},
  "ISSN": [
    "0969-2126"
  ],
  "subject": [],
  "container-title-short": "Structure",
  "published": {
    "date-parts": [
      [
        2005,
        9
      ]
    ]
  }
}