DOI: 10.1016/j.sbi.2013.12.002. Structural studies of the spliceosome: zooming into the heart of the machine

Structural studies of the spliceosome: zooming into the heart of the machine

10.1016/j.sbi.2013.12.002

Crossref journal-article

Elsevier BV

Current Opinion in Structural Biology (78)

Bibliography

Galej, W. P., Nguyen, T. H. D., Newman, A. J., & Nagai, K. (2014). Structural studies of the spliceosome: zooming into the heart of the machine. Current Opinion in Structural Biology, 25, 57â66.

Authors 4

Wojciech P Galej (first)
Thi Hoang Duong Nguyen (additional)
Andrew J Newman (additional)
Kiyoshi Nagai (additional)

References 61 Referenced 50

10.1016/j.cell.2009.02.009 / Cell / The spliceosome: design principles of a dynamic RNP machine by Wahl (2009)
10.1101/cshperspect.a003707 / Cold Spring Harb Perspect Biol / Spliceosome structure and function by Will (2011)
10.1016/j.ceb.2012.03.004 / Curr Opin Cell Biol / Structure, function and regulation of spliceosomal RNA helicases by Cordin (2012)
10.1016/j.sbi.2009.12.003 / Curr Opin Struct Biol / Structural studies of the spliceosome: blind men and an elephant by Newman (2010)
10.1016/j.sbi.2009.01.001 / Curr Opin Struct Biol / Structural mapping of spliceosomes by electron microscopy by Lührmann (2009)
10.1038/nature07851 / Nature / Crystal structure of human spliceosomal U1 snRNP at 5.5A resolution by Pomeranz Krummel (2009)
10.1038/emboj.2010.295 / EMBO J / Functional organization of the Sm core in the crystal structure of human U1 snRNP by Weber (2010)
10.1038/nature09956 / Nature / Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis by Leung (2011)
10.1038/nature11843 / Nature / Crystal structure of Prp8 reveals active site cavity of the spliceosome by Galej (2013)
10.1073/pnas.1208098109 / Proc Natl Acad Sci U S A / Structural basis for functional cooperation between tandem helicase cassettes in Brr2-mediated remodeling of the spliceosome by Santos (2012)
10.1016/j.str.2013.04.017 / Structure / Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site by Nguyen (2013)
10.1126/science.1237515 / Science / Inhibition of RNA helicase Brr2 by the C-terminal tail of the spliceosomal protein Prp8 by Mozaffari-Jovin (2013)
10.1093/genetics/157.4.1451 / Genetics / Functional contacts with a range of splicing proteins suggest a central role for Brr2p in the dynamic control of the order of events in spliceosomes of Saccharomyces cerevisiae by van Nues (2001)
10.1261/rna.55406 / RNA / The network of protein–protein interactions within the human U4/U6.U5 tri-snRNP by Liu (2006)
10.1261/rna.2229706 / RNA / Dissection of Prp8 protein defines multiple interactions with crucial RNA sequences in the catalytic core of the spliceosome by Turner (2006)
10.1017/S1355838298981109 / RNA / Protein–RNA interactions in the U5 snRNP of Saccharomyces cerevisiae by Dix (1998)
10.1017/S1355838299981785 / RNA / The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5′ splice site by Reyes (1999)
10.1017/S1355838299991355 / RNA / Characterization of U6 snRNA–protein interactions by Vidal (1999)
10.1261/rna.2220705 / RNA / Prp8 protein: at the heart of the spliceosome by Grainger (2005)
10.1093/genetics/143.2.723 / Genetics / Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3′ splice site selection by Umen (1996)
10.1101/gad.13.15.1970 / Genes Dev / Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome by Collins (1999)
10.1038/nsmb1240 / Nat Struct Mol Biol / Opposing classes of prp8 alleles modulate the transition between the catalytic steps of pre-mRNA splicing by Liu (2007)
10.1016/j.molcel.2007.01.023 / Mol Cell / Structure of a multipartite protein–protein interaction domain in splicing factor prp8 and its link to retinitis pigmentosa by Pena (2007)
10.1110/ps.072872007 / Protein Sci / Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants by Zhang (2007)
10.1038/emboj.2008.209 / EMBO J / Structure and function of an RNase H domain at the heart of the spliceosome by Pena (2008)
10.1038/nsmb.1505 / Nat Struct Mol Biol / Structural elucidation of a PRP8 core domain from the heart of the spliceosome by Ritchie (2008)
10.1073/pnas.0805960105 / Proc Natl Acad Sci U S A / Crystal structure of the beta-finger domain of Prp8 reveals analogy to ribosomal proteins by Yang (2008)
10.1038/nsmb1208-1235 / Nat Struct Mol Biol / Is the spliceosome a ribonucleoprotein enzyme? by Abelson (2008)
10.1016/j.cell.2005.04.024 / Cell / Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis by Nowotny (2005)
10.1073/pnas.90.14.6498 / Proc Natl Acad Sci U S A / A general two-metal-ion mechanism for catalytic RNA by Steitz (1993)
10.1038/nsmb.2556 / Nat Struct Mol Biol / A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation by Schellenberg (2013)
10.1038/nsmb.2603 / Nat Struct Mol Biol / Toggling in the spliceosome by Abelson (2013)
10.1261/rna.2396011 / RNA / Prp8, the pivotal protein of the spliceosomal catalytic center, evolved from a retroelement-encoded reverse transcriptase by Dlakić (2011)
{'key': '10.1016/j.sbi.2013.12.002_bib0170', 'series-title': 'RNA Structure and Function', 'first-page': '279', 'article-title': 'RNA–RNA interactions in nuclear Pre-mRNA splicing', 'author': 'Nilsen', 'year': '1998'} / RNA Structure and Function / RNA–RNA interactions in nuclear Pre-mRNA splicing by Nilsen (1998)
10.1038/35048617 / Nature / Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome by Yean (2000)
10.1038/nsb800 / Nat Struct Biol / Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure by Huppler (2002)
10.1038/nature12734 / Nature / RNA catalyzes nuclear pre-mRNA splicing by Fica (2013)
10.1261/rna.031138.111 / RNA / Structure of the yeast U2/U6 snRNA complex by Burke (2012)
10.1126/science.1153803 / Science / Crystal structure of a self-spliced group II intron by Toor (2008)
10.1261/rna.1791310 / RNA / A structural analysis of the group II intron active site and implications for the spliceosome by Keating (2010)
10.1038/emboj.2013.198 / EMBO J / RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core by Anokhina (2013)
10.1016/j.cell.2012.09.033 / Cell / Visualizing group II intron catalysis through the stages of splicing by Marcia (2012)
10.1038/emboj.2011.502 / EMBO J / Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre by Rasche (2012)
10.1042/BJ20111385 / Biochem J / Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition by Lu (2012)
10.1038/emboj.2012.58 / EMBO J / Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein by Schmitzová (2012)
10.1017/S135583829999012X / RNA / The first ATPase domain of the yeast 246-kDa protein is required for in vivo unwinding of the U4/U6 duplex by Kim (1999)
10.1038/nsmb1246 / Nat Struct Mol Biol / Structural basis for DNA duplex separation by a superfamily-2 helicase by Büttner (2007)
10.1038/nsmb.1625 / Nat Struct Mol Biol / Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2 by Zhang (2009)
10.1016/j.molcel.2006.05.043 / Mol Cell / The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase by Small (2006)
10.1074/jbc.M303043200 / J Biol Chem / Mutagenesis suggests several roles of Snu114p in pre-mRNA splicing by Bartels (2003)
10.1038/nsmb.1535 / Nat Struct Mol Biol / ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8 by Maeder (2009)
10.1101/gad.200949.112 / Genes Dev / The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA by Mozaffari-Jovin (2012)
10.1101/gad.199307.112 / Genes Dev / Brr2p-mediated conformational rearrangements in the spliceosome during activation and substrate repositioning by Hahn (2012)
10.1093/genetics/155.4.1667 / Genetics / Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation by Kuhn (2000)
10.1038/nsmb1303 / Nat Struct Mol Biol / prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast by Boon (2007)
10.1101/gad.635911 / Genes Dev / Mechanism for Aar2p function as a U5 snRNP assembly factor by Weber (2011)
10.1016/0092-8674(85)90092-3 / Cell / On the origin of RNA splicing and introns by Sharp (1985)
10.1016/0092-8674(86)90751-8 / Cell / The generality of self-splicing RNA: relationship to nuclear mRNA splicing by Cech (1986)
10.1101/cshperspect.a003616 / Cold Spring Harb Perspect Biol / Group II introns: mobile ribozymes that invade DNA by Lambowitz (2011)
10.1126/science.1948046 / Science / Five easy pieces by Sharp (1991)
10.7554/eLife.00461 / Elife / Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles by Bai (2013)

Dates

Type	When
Created	11 years, 7 months ago (Jan. 27, 2014, 9:46 p.m.)
Deposited	3 years, 5 months ago (March 24, 2022, 11:10 a.m.)
Indexed	2 weeks ago (Aug. 23, 2025, 9:22 p.m.)
Issued	11 years, 5 months ago (April 1, 2014)
Published	11 years, 5 months ago (April 1, 2014)
Published Print	11 years, 5 months ago (April 1, 2014)

Funders 1

Medical Research Council

BibTeX

@article{Galej_2014, title={Structural studies of the spliceosome: zooming into the heart of the machine}, volume={25}, ISSN={0959-440X}, url={http://dx.doi.org/10.1016/j.sbi.2013.12.002}, DOI={10.1016/j.sbi.2013.12.002}, journal={Current Opinion in Structural Biology}, publisher={Elsevier BV}, author={Galej, Wojciech P and Nguyen, Thi Hoang Duong and Newman, Andrew J and Nagai, Kiyoshi}, year={2014}, month=apr, pages={57–66} }

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      "author": "Ritchie",
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      "DOI": "10.1038/nsmb1208-1235",
      "article-title": "Is the spliceosome a ribonucleoprotein enzyme?",
      "volume": "15",
      "author": "Abelson",
      "year": "2008",
      "journal-title": "Nat Struct Mol Biol"
    },
    {
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      "first-page": "1005",
      "DOI": "10.1016/j.cell.2005.04.024",
      "article-title": "Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis",
      "volume": "121",
      "author": "Nowotny",
      "year": "2005",
      "journal-title": "Cell"
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    {
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      "first-page": "6498",
      "DOI": "10.1073/pnas.90.14.6498",
      "article-title": "A general two-metal-ion mechanism for catalytic RNA",
      "volume": "90",
      "author": "Steitz",
      "year": "1993",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
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      "doi-asserted-by": "crossref",
      "first-page": "728",
      "DOI": "10.1038/nsmb.2556",
      "article-title": "A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation",
      "volume": "20",
      "author": "Schellenberg",
      "year": "2013",
      "journal-title": "Nat Struct Mol Biol"
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      "first-page": "645",
      "DOI": "10.1038/nsmb.2603",
      "article-title": "Toggling in the spliceosome",
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      "author": "Abelson",
      "year": "2013",
      "journal-title": "Nat Struct Mol Biol"
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    {
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      "first-page": "799",
      "DOI": "10.1261/rna.2396011",
      "article-title": "Prp8, the pivotal protein of the spliceosomal catalytic center, evolved from a retroelement-encoded reverse transcriptase",
      "volume": "17",
      "author": "Dlaki\u0107",
      "year": "2011",
      "journal-title": "RNA"
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      "key": "10.1016/j.sbi.2013.12.002_bib0170",
      "series-title": "RNA Structure and Function",
      "first-page": "279",
      "article-title": "RNA\u2013RNA interactions in nuclear Pre-mRNA splicing",
      "author": "Nilsen",
      "year": "1998"
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      "first-page": "881",
      "DOI": "10.1038/35048617",
      "article-title": "Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome",
      "volume": "408",
      "author": "Yean",
      "year": "2000",
      "journal-title": "Nature"
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      "first-page": "431",
      "DOI": "10.1038/nsb800",
      "article-title": "Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure",
      "volume": "9",
      "author": "Huppler",
      "year": "2002",
      "journal-title": "Nat Struct Biol"
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      "doi-asserted-by": "crossref",
      "first-page": "229",
      "DOI": "10.1038/nature12734",
      "article-title": "RNA catalyzes nuclear pre-mRNA splicing",
      "volume": "503",
      "author": "Fica",
      "year": "2013",
      "journal-title": "Nature"
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      "first-page": "673",
      "DOI": "10.1261/rna.031138.111",
      "article-title": "Structure of the yeast U2/U6 snRNA complex",
      "volume": "18",
      "author": "Burke",
      "year": "2012",
      "journal-title": "RNA"
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      "doi-asserted-by": "crossref",
      "first-page": "77",
      "DOI": "10.1126/science.1153803",
      "article-title": "Crystal structure of a self-spliced group II intron",
      "volume": "320",
      "author": "Toor",
      "year": "2008",
      "journal-title": "Science"
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      "first-page": "1",
      "DOI": "10.1261/rna.1791310",
      "article-title": "A structural analysis of the group II intron active site and implications for the spliceosome",
      "volume": "16",
      "author": "Keating",
      "year": "2010",
      "journal-title": "RNA"
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      "first-page": "2804",
      "DOI": "10.1038/emboj.2013.198",
      "article-title": "RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core",
      "volume": "32",
      "author": "Anokhina",
      "year": "2013",
      "journal-title": "EMBO J"
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      "first-page": "497",
      "DOI": "10.1016/j.cell.2012.09.033",
      "article-title": "Visualizing group II intron catalysis through the stages of splicing",
      "volume": "151",
      "author": "Marcia",
      "year": "2012",
      "journal-title": "Cell"
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      "first-page": "1591",
      "DOI": "10.1038/emboj.2011.502",
      "article-title": "Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre",
      "volume": "31",
      "author": "Rasche",
      "year": "2012",
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      "first-page": "591",
      "DOI": "10.1042/BJ20111385",
      "article-title": "Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition",
      "volume": "441",
      "author": "Lu",
      "year": "2012",
      "journal-title": "Biochem J"
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    {
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      "first-page": "2222",
      "DOI": "10.1038/emboj.2012.58",
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      "volume": "31",
      "author": "Schmitzov\u00e1",
      "year": "2012",
      "journal-title": "EMBO J"
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    {
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      "first-page": "959",
      "DOI": "10.1017/S135583829999012X",
      "article-title": "The first ATPase domain of the yeast 246-kDa protein is required for in vivo unwinding of the U4/U6 duplex",
      "volume": "5",
      "author": "Kim",
      "year": "1999",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.sbi.2013.12.002_bib0235",
      "doi-asserted-by": "crossref",
      "first-page": "647",
      "DOI": "10.1038/nsmb1246",
      "article-title": "Structural basis for DNA duplex separation by a superfamily-2 helicase",
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      "author": "B\u00fcttner",
      "year": "2007",
      "journal-title": "Nat Struct Mol Biol"
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      "first-page": "731",
      "DOI": "10.1038/nsmb.1625",
      "article-title": "Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2",
      "volume": "16",
      "author": "Zhang",
      "year": "2009",
      "journal-title": "Nat Struct Mol Biol"
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    {
      "key": "10.1016/j.sbi.2013.12.002_bib0245",
      "doi-asserted-by": "crossref",
      "first-page": "389",
      "DOI": "10.1016/j.molcel.2006.05.043",
      "article-title": "The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase",
      "volume": "23",
      "author": "Small",
      "year": "2006",
      "journal-title": "Mol Cell"
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    {
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      "doi-asserted-by": "crossref",
      "first-page": "28324",
      "DOI": "10.1074/jbc.M303043200",
      "article-title": "Mutagenesis suggests several roles of Snu114p in pre-mRNA splicing",
      "volume": "278",
      "author": "Bartels",
      "year": "2003",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1016/j.sbi.2013.12.002_bib0255",
      "doi-asserted-by": "crossref",
      "first-page": "42",
      "DOI": "10.1038/nsmb.1535",
      "article-title": "ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8",
      "volume": "16",
      "author": "Maeder",
      "year": "2009",
      "journal-title": "Nat Struct Mol Biol"
    },
    {
      "key": "10.1016/j.sbi.2013.12.002_bib0260",
      "doi-asserted-by": "crossref",
      "first-page": "2422",
      "DOI": "10.1101/gad.200949.112",
      "article-title": "The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA",
      "volume": "26",
      "author": "Mozaffari-Jovin",
      "year": "2012",
      "journal-title": "Genes Dev"
    },
    {
      "key": "10.1016/j.sbi.2013.12.002_bib0265",
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      "first-page": "2408",
      "DOI": "10.1101/gad.199307.112",
      "article-title": "Brr2p-mediated conformational rearrangements in the spliceosome during activation and substrate repositioning",
      "volume": "26",
      "author": "Hahn",
      "year": "2012",
      "journal-title": "Genes Dev"
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    {
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      "first-page": "1667",
      "DOI": "10.1093/genetics/155.4.1667",
      "article-title": "Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation",
      "volume": "155",
      "author": "Kuhn",
      "year": "2000",
      "journal-title": "Genetics"
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    {
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      "first-page": "1077",
      "DOI": "10.1038/nsmb1303",
      "article-title": "prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast",
      "volume": "14",
      "author": "Boon",
      "year": "2007",
      "journal-title": "Nat Struct Mol Biol"
    },
    {
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      "doi-asserted-by": "crossref",
      "first-page": "1601",
      "DOI": "10.1101/gad.635911",
      "article-title": "Mechanism for Aar2p function as a U5 snRNP assembly factor",
      "volume": "25",
      "author": "Weber",
      "year": "2011",
      "journal-title": "Genes Dev"
    },
    {
      "key": "10.1016/j.sbi.2013.12.002_bib0285",
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      "first-page": "397",
      "DOI": "10.1016/0092-8674(85)90092-3",
      "article-title": "On the origin of RNA splicing and introns",
      "volume": "42",
      "author": "Sharp",
      "year": "1985",
      "journal-title": "Cell"
    },
    {
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      "first-page": "207",
      "DOI": "10.1016/0092-8674(86)90751-8",
      "article-title": "The generality of self-splicing RNA: relationship to nuclear mRNA splicing",
      "volume": "44",
      "author": "Cech",
      "year": "1986",
      "journal-title": "Cell"
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    {
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      "first-page": "a003616",
      "DOI": "10.1101/cshperspect.a003616",
      "article-title": "Group II introns: mobile ribozymes that invade DNA",
      "volume": "3",
      "author": "Lambowitz",
      "year": "2011",
      "journal-title": "Cold Spring Harb Perspect Biol"
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      "first-page": "663",
      "DOI": "10.1126/science.1948046",
      "article-title": "Five easy pieces",
      "volume": "254",
      "author": "Sharp",
      "year": "1991",
      "journal-title": "Science"
    },
    {
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      "first-page": "e00461",
      "DOI": "10.7554/eLife.00461",
      "article-title": "Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles",
      "volume": "2",
      "author": "Bai",
      "year": "2013",
      "journal-title": "Elife"
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