DOI: 10.1016/j.molcel.2014.01.017. A Splicing-Dependent Transcriptional Checkpoint Associated with Prespliceosome Formation

A Splicing-Dependent Transcriptional Checkpoint Associated with Prespliceosome Formation

10.1016/j.molcel.2014.01.017

Crossref journal-article

Elsevier BV

Molecular Cell (78)

Bibliography

Chathoth, K. T., Barrass, J. D., Webb, S., & Beggs, J. D. (2014). A Splicing-Dependent Transcriptional Checkpoint Associated with Prespliceosome Formation. Molecular Cell, 53(5), 779â790.

Authors 4

Keerthi T. Chathoth (first)
J. David Barrass (additional)
Shaun Webb (additional)
Jean D. Beggs (additional)

References 59 Referenced 86

10.1016/S1097-2765(03)00492-1 / Mol. Cell / Phosphorylation of serine 2 within the RNA polymerase II C-terminal domain couples transcription and 3′ end processing by Ahn (2004)
10.1371/journal.pcbi.1002215 / PLoS Comput. Biol. / Modelling reveals kinetic advantages of co-transcriptional splicing by Aitken (2011)
10.1016/j.molcel.2010.11.005 / Mol. Cell / Splicing-dependent RNA polymerase pausing in yeast by Alexander (2010)
10.1261/rna.2162610 / RNA / RiboSys, a high-resolution, quantitative approach to measure the in vivo kinetics of pre-mRNA splicing and 3′-end processing in Saccharomyces cerevisiae by Alexander (2010)
10.1038/nsmb1030 / Nat. Struct. Mol. Biol. / The human SWI/SNF subunit Brm is a regulator of alternative splicing by Batsché (2006)
10.1016/j.ceb.2005.04.006 / Curr. Opin. Cell Biol. / Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors by Bentley (2005)
10.1016/j.molcel.2009.10.019 / Mol. Cell / Progression through the RNA polymerase II CTD cycle by Buratowski (2009)
10.1016/j.molcel.2010.11.004 / Mol. Cell / Global analysis of nascent RNA reveals transcriptional pausing in terminal exons by Carrillo Oesterreich (2010)
10.1126/science.1145977 / Science / Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7 by Chapman (2007)
10.1101/gad.1834709 / Genes Dev. / DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation by Chen (2009)
10.1101/gad.11.24.3319 / Genes Dev. / mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain by Cho (1997)
10.4161/rna.22547 / RNA Biol. / RNA helicases in splicing by Cordin (2013)
10.1016/S1097-2765(00)80372-X / Mol. Cell / Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer by Cramer (1999)
10.1016/j.molcel.2007.11.035 / Mol. Cell / A 5′ splice site enhances the recruitment of basal transcription initiation factors in vivo by Damgaard (2008)
10.1016/j.molcel.2007.05.036 / Mol. Cell / SR proteins function in coupling RNAP II transcription to pre-mRNA splicing by Das (2007)
10.4161/trns.2.5.17272 / Transcription / The RNA polymerase C-terminal domain: a new role in spliceosome assembly by David (2011)
10.1093/nar/gkq703 / Nucleic Acids Res. / A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination by de Almeida (2010)
10.1261/rna.7186504 / RNA / Modified nucleotides at the 5′ end of human U2 snRNA are required for spliceosomal E-complex formation by Dönmez (2004)
10.1093/nar/30.1.207 / Nucleic Acids Res. / Gene Expression Omnibus: NCBI gene expression and hybridization array data repository by Edgar (2002)
10.1016/j.tig.2008.03.008 / Trends Genet. / Cracking the RNA polymerase II CTD code by Egloff (2008)
10.1038/414929a / Nature / Stimulatory effect of splicing factors on transcriptional elongation by Fong (2001)
10.1101/gad.983602 / Genes Dev. / Promoter proximal splice sites enhance transcription by Furger (2002)
10.1016/j.molcel.2005.05.007 / Mol. Cell / Cotranscriptional spliceosome assembly occurs in a stepwise fashion and requires the cap binding complex by Görnemann (2005)
10.1038/25786 / Nature / RNA polymerase II is an essential mRNA polyadenylation factor by Hirose (1998)
10.1016/j.cell.2013.04.028 / Cell / SR proteins collaborate with 7SK and promoter-associated nascent RNA to release paused polymerase by Ji (2013)
10.1038/sj.emboj.7600187 / EMBO J. / p54(nrb) associates with the 5′ splice site within large transcription/splicing complexes by Kameoka (2004)
10.1101/gad.824700 / Genes Dev. / Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription by Komarnitsky (2000)
10.1261/rna.7100104 / RNA / Multiple links between transcription and splicing by Kornblihtt (2004)
10.1038/nrm3525 / Nat. Rev. Mol. Cell Biol. / Alternative splicing: a pivotal step between eukaryotic transcription and translation by Kornblihtt (2013)
10.1261/rna.1065209 / RNA / DExD/H-box Prp5 protein is in the spliceosome during most of the splicing cycle by Kosowski (2009)
10.1016/j.molcel.2005.05.006 / Mol. Cell / Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5’ss base pairing in yeast by Lacadie (2005)
10.1016/j.cell.2011.04.021 / Cell / Paused RNA polymerase II as a developmental checkpoint by Levine (2011)
10.1016/j.gde.2011.01.010 / Curr. Opin. Genet. Dev. / Promoter proximal pausing and the control of gene expression by Li (2011)
10.1038/nsmb.1461 / Nat. Struct. Mol. Biol. / The splicing factor SC35 has an active role in transcriptional elongation by Lin (2008)
10.1038/385357a0 / Nature / The C-terminal domain of RNA polymerase II couples mRNA processing to transcription by McCracken (1997)
10.1371/journal.pone.0016077 / PLoS ONE / An investigation of a role for U2 snRNP spliceosomal components in regulating transcription by McKay (2011)
10.1261/rna.2462011 / RNA / Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing by Miller (2011)
10.1016/j.cell.2009.02.001 / Cell / Pre-mRNA processing reaches back to transcription and ahead to translation by Moore (2009)
10.1016/S0092-8674(02)00655-4 / Cell / A unified theory of gene expression by Orphanides (2002)
10.1101/gad.14.1.97 / Genes Dev. / ATP can be dispensable for prespliceosome formation in yeast by Perriman (2000)
10.1101/gad.1524307 / Genes Dev. / Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing by Perriman (2007)
10.1016/j.molcel.2010.02.036 / Mol. Cell / Invariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing by Perriman (2010)
10.1073/pnas.2036312100 / Proc. Natl. Acad. Sci. USA / ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA by Perriman (2003)
10.1371/journal.pbio.0050090 / PLoS Biol. / Transcript specificity in yeast pre-mRNA splicing revealed by mutations in core spliceosomal components by Pleiss (2007)
10.1101/gad.7.10.1909 / Genes Dev. / Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA by Ruby (1993)
10.1016/j.ymeth.2009.03.001 / Methods / ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions by Schmidt (2009)
10.1074/jbc.M312715200 / J. Biol. Chem. / Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8 by Schneider (2004)
10.1101/gad.836300 / Genes Dev. / Dynamic association of capping enzymes with transcribing RNA polymerase II by Schroeder (2000)
10.1128/MCB.06234-11 / Mol. Cell. Biol. / A U1-U2 snRNP interaction network during intron definition by Shao (2012)
10.1038/nsmb.1666 / Nat. Struct. Mol. Biol. / Rates of in situ transcription and splicing in large human genes by Singh (2009)
10.1261/rna.50506 / RNA / Arrested yeast splicing complexes indicate stepwise snRNP recruitment during in vivo spliceosome assembly by Tardiff (2006)
10.1016/j.cell.2009.02.009 / Cell / The spliceosome: design principles of a dynamic RNP machine by Wahl (2009)
10.1101/gad.10.2.220 / Genes Dev. / CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145 by Wells (1996)
10.1016/j.tig.2010.12.001 / Trends Genet. / Understanding splicing regulation through RNA splicing maps by Witten (2011)
10.1016/j.molcel.2007.09.022 / Mol. Cell / Competition between the ATPase Prp5 and branch region-U2 snRNA pairing modulates the fidelity of spliceosome assembly by Xu (2007)
10.1038/sj.emboj.7600050 / EMBO J. / Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA by Xu (2004)
10.1128/MCB.18.9.5000 / Mol. Cell. Biol. / CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif by Yan (1998)
10.1101/gad.5.12b.2521 / Genes Dev. / Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural element by Zavanelli (1991)
10.1126/science.274.5287.605 / Science / Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat by Zhou (1996)

Dates

Type	When
Created	11 years, 6 months ago (Feb. 20, 2014, 2 p.m.)
Deposited	3 years, 4 months ago (March 27, 2022, 12:48 a.m.)
Indexed	1 month, 3 weeks ago (July 1, 2025, 1:34 p.m.)
Issued	11 years, 5 months ago (March 1, 2014)
Published	11 years, 5 months ago (March 1, 2014)
Published Print	11 years, 5 months ago (March 1, 2014)

Funders 0

None

BibTeX

@article{Chathoth_2014, title={A Splicing-Dependent Transcriptional Checkpoint Associated with Prespliceosome Formation}, volume={53}, ISSN={1097-2765}, url={http://dx.doi.org/10.1016/j.molcel.2014.01.017}, DOI={10.1016/j.molcel.2014.01.017}, number={5}, journal={Molecular Cell}, publisher={Elsevier BV}, author={Chathoth, Keerthi T. and Barrass, J. David and Webb, Shaun and Beggs, Jean D.}, year={2014}, month=mar, pages={779–790} }

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      "article-title": "RiboSys, a high-resolution, quantitative approach to measure the in\u00a0vivo kinetics of pre-mRNA splicing and 3\u2032-end processing in Saccharomyces cerevisiae",
      "volume": "16",
      "author": "Alexander",
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      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib5",
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      "first-page": "22",
      "DOI": "10.1038/nsmb1030",
      "article-title": "The human SWI/SNF subunit Brm is a regulator of alternative splicing",
      "volume": "13",
      "author": "Batsch\u00e9",
      "year": "2006",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
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      "first-page": "251",
      "DOI": "10.1016/j.ceb.2005.04.006",
      "article-title": "Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors",
      "volume": "17",
      "author": "Bentley",
      "year": "2005",
      "journal-title": "Curr. Opin. Cell Biol."
    },
    {
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      "first-page": "541",
      "DOI": "10.1016/j.molcel.2009.10.019",
      "article-title": "Progression through the RNA polymerase II CTD cycle",
      "volume": "36",
      "author": "Buratowski",
      "year": "2009",
      "journal-title": "Mol. Cell"
    },
    {
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      "first-page": "571",
      "DOI": "10.1016/j.molcel.2010.11.004",
      "article-title": "Global analysis of nascent RNA reveals transcriptional pausing in terminal exons",
      "volume": "40",
      "author": "Carrillo Oesterreich",
      "year": "2010",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "1780",
      "DOI": "10.1126/science.1145977",
      "article-title": "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7",
      "volume": "318",
      "author": "Chapman",
      "year": "2007",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib10",
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      "volume": "23",
      "author": "Chen",
      "year": "2009",
      "journal-title": "Genes Dev."
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      "volume": "11",
      "author": "Cho",
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      "journal-title": "Genes Dev."
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    {
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      "first-page": "83",
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      "volume": "10",
      "author": "Cordin",
      "year": "2013",
      "journal-title": "RNA Biol."
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      "volume": "4",
      "author": "Cramer",
      "year": "1999",
      "journal-title": "Mol. Cell"
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      "volume": "29",
      "author": "Damgaard",
      "year": "2008",
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      "volume": "26",
      "author": "Das",
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      "volume": "2",
      "author": "David",
      "year": "2011",
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      "volume": "38",
      "author": "de Almeida",
      "year": "2010",
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      "volume": "10",
      "author": "D\u00f6nmez",
      "year": "2004",
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      "volume": "30",
      "author": "Edgar",
      "year": "2002",
      "journal-title": "Nucleic Acids Res."
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      "first-page": "280",
      "DOI": "10.1016/j.tig.2008.03.008",
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      "volume": "24",
      "author": "Egloff",
      "year": "2008",
      "journal-title": "Trends Genet."
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    {
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      "first-page": "929",
      "DOI": "10.1038/414929a",
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      "volume": "414",
      "author": "Fong",
      "year": "2001",
      "journal-title": "Nature"
    },
    {
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      "DOI": "10.1101/gad.983602",
      "article-title": "Promoter proximal splice sites enhance transcription",
      "volume": "16",
      "author": "Furger",
      "year": "2002",
      "journal-title": "Genes Dev."
    },
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      "first-page": "53",
      "DOI": "10.1016/j.molcel.2005.05.007",
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      "volume": "19",
      "author": "G\u00f6rnemann",
      "year": "2005",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib24",
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      "first-page": "93",
      "DOI": "10.1038/25786",
      "article-title": "RNA polymerase II is an essential mRNA polyadenylation factor",
      "volume": "395",
      "author": "Hirose",
      "year": "1998",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "855",
      "DOI": "10.1016/j.cell.2013.04.028",
      "article-title": "SR proteins collaborate with 7SK and promoter-associated nascent RNA to release paused polymerase",
      "volume": "153",
      "author": "Ji",
      "year": "2013",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "1782",
      "DOI": "10.1038/sj.emboj.7600187",
      "article-title": "p54(nrb) associates with the 5\u2032 splice site within large transcription/splicing complexes",
      "volume": "23",
      "author": "Kameoka",
      "year": "2004",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "2452",
      "DOI": "10.1101/gad.824700",
      "article-title": "Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription",
      "volume": "14",
      "author": "Komarnitsky",
      "year": "2000",
      "journal-title": "Genes Dev."
    },
    {
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      "doi-asserted-by": "crossref",
      "first-page": "1489",
      "DOI": "10.1261/rna.7100104",
      "article-title": "Multiple links between transcription and splicing",
      "volume": "10",
      "author": "Kornblihtt",
      "year": "2004",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib29",
      "doi-asserted-by": "crossref",
      "first-page": "153",
      "DOI": "10.1038/nrm3525",
      "article-title": "Alternative splicing: a pivotal step between eukaryotic transcription and translation",
      "volume": "14",
      "author": "Kornblihtt",
      "year": "2013",
      "journal-title": "Nat. Rev. Mol. Cell Biol."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "1345",
      "DOI": "10.1261/rna.1065209",
      "article-title": "DExD/H-box Prp5 protein is in the spliceosome during most of the splicing cycle",
      "volume": "15",
      "author": "Kosowski",
      "year": "2009",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "65",
      "DOI": "10.1016/j.molcel.2005.05.006",
      "article-title": "Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5\u2019ss base pairing in yeast",
      "volume": "19",
      "author": "Lacadie",
      "year": "2005",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib32",
      "doi-asserted-by": "crossref",
      "first-page": "502",
      "DOI": "10.1016/j.cell.2011.04.021",
      "article-title": "Paused RNA polymerase II as a developmental checkpoint",
      "volume": "145",
      "author": "Levine",
      "year": "2011",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "231",
      "DOI": "10.1016/j.gde.2011.01.010",
      "article-title": "Promoter proximal pausing and the control of gene expression",
      "volume": "21",
      "author": "Li",
      "year": "2011",
      "journal-title": "Curr. Opin. Genet. Dev."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "819",
      "DOI": "10.1038/nsmb.1461",
      "article-title": "The splicing factor SC35 has an active role in transcriptional elongation",
      "volume": "15",
      "author": "Lin",
      "year": "2008",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "357",
      "DOI": "10.1038/385357a0",
      "article-title": "The C-terminal domain of RNA polymerase II couples mRNA processing to transcription",
      "volume": "385",
      "author": "McCracken",
      "year": "1997",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib36",
      "doi-asserted-by": "crossref",
      "first-page": "e16077",
      "DOI": "10.1371/journal.pone.0016077",
      "article-title": "An investigation of a role for U2 snRNP spliceosomal components in regulating transcription",
      "volume": "6",
      "author": "McKay",
      "year": "2011",
      "journal-title": "PLoS ONE"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib37",
      "doi-asserted-by": "crossref",
      "first-page": "665",
      "DOI": "10.1261/rna.2462011",
      "article-title": "Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing",
      "volume": "17",
      "author": "Miller",
      "year": "2011",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib38",
      "doi-asserted-by": "crossref",
      "first-page": "688",
      "DOI": "10.1016/j.cell.2009.02.001",
      "article-title": "Pre-mRNA processing reaches back to transcription and ahead to translation",
      "volume": "136",
      "author": "Moore",
      "year": "2009",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib39",
      "doi-asserted-by": "crossref",
      "first-page": "439",
      "DOI": "10.1016/S0092-8674(02)00655-4",
      "article-title": "A unified theory of gene expression",
      "volume": "108",
      "author": "Orphanides",
      "year": "2002",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib40",
      "doi-asserted-by": "crossref",
      "first-page": "97",
      "DOI": "10.1101/gad.14.1.97",
      "article-title": "ATP can be dispensable for prespliceosome formation in yeast",
      "volume": "14",
      "author": "Perriman",
      "year": "2000",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib41",
      "doi-asserted-by": "crossref",
      "first-page": "811",
      "DOI": "10.1101/gad.1524307",
      "article-title": "Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing",
      "volume": "21",
      "author": "Perriman",
      "year": "2007",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib42",
      "doi-asserted-by": "crossref",
      "first-page": "416",
      "DOI": "10.1016/j.molcel.2010.02.036",
      "article-title": "Invariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing",
      "volume": "38",
      "author": "Perriman",
      "year": "2010",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib43",
      "doi-asserted-by": "crossref",
      "first-page": "13857",
      "DOI": "10.1073/pnas.2036312100",
      "article-title": "ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA",
      "volume": "100",
      "author": "Perriman",
      "year": "2003",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib44",
      "doi-asserted-by": "crossref",
      "first-page": "e90",
      "DOI": "10.1371/journal.pbio.0050090",
      "article-title": "Transcript specificity in yeast pre-mRNA splicing revealed by mutations in core spliceosomal components",
      "volume": "5",
      "author": "Pleiss",
      "year": "2007",
      "journal-title": "PLoS Biol."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib45",
      "doi-asserted-by": "crossref",
      "first-page": "1909",
      "DOI": "10.1101/gad.7.10.1909",
      "article-title": "Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA",
      "volume": "7",
      "author": "Ruby",
      "year": "1993",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib46",
      "doi-asserted-by": "crossref",
      "first-page": "240",
      "DOI": "10.1016/j.ymeth.2009.03.001",
      "article-title": "ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions",
      "volume": "48",
      "author": "Schmidt",
      "year": "2009",
      "journal-title": "Methods"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib47",
      "doi-asserted-by": "crossref",
      "first-page": "8617",
      "DOI": "10.1074/jbc.M312715200",
      "article-title": "Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8",
      "volume": "279",
      "author": "Schneider",
      "year": "2004",
      "journal-title": "J.\u00a0Biol. Chem."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib48",
      "doi-asserted-by": "crossref",
      "first-page": "2435",
      "DOI": "10.1101/gad.836300",
      "article-title": "Dynamic association of capping enzymes with transcribing RNA polymerase II",
      "volume": "14",
      "author": "Schroeder",
      "year": "2000",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib49",
      "doi-asserted-by": "crossref",
      "first-page": "470",
      "DOI": "10.1128/MCB.06234-11",
      "article-title": "A U1-U2 snRNP interaction network during intron definition",
      "volume": "32",
      "author": "Shao",
      "year": "2012",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib50",
      "doi-asserted-by": "crossref",
      "first-page": "1128",
      "DOI": "10.1038/nsmb.1666",
      "article-title": "Rates of in\u00a0situ transcription and splicing in large human genes",
      "volume": "16",
      "author": "Singh",
      "year": "2009",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib51",
      "doi-asserted-by": "crossref",
      "first-page": "968",
      "DOI": "10.1261/rna.50506",
      "article-title": "Arrested yeast splicing complexes indicate stepwise snRNP recruitment during in\u00a0vivo spliceosome assembly",
      "volume": "12",
      "author": "Tardiff",
      "year": "2006",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib52",
      "doi-asserted-by": "crossref",
      "first-page": "701",
      "DOI": "10.1016/j.cell.2009.02.009",
      "article-title": "The spliceosome: design principles of a dynamic RNP machine",
      "volume": "136",
      "author": "Wahl",
      "year": "2009",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib53",
      "doi-asserted-by": "crossref",
      "first-page": "220",
      "DOI": "10.1101/gad.10.2.220",
      "article-title": "CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145",
      "volume": "10",
      "author": "Wells",
      "year": "1996",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib54",
      "doi-asserted-by": "crossref",
      "first-page": "89",
      "DOI": "10.1016/j.tig.2010.12.001",
      "article-title": "Understanding splicing regulation through RNA splicing maps",
      "volume": "27",
      "author": "Witten",
      "year": "2011",
      "journal-title": "Trends Genet."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib55",
      "doi-asserted-by": "crossref",
      "first-page": "838",
      "DOI": "10.1016/j.molcel.2007.09.022",
      "article-title": "Competition between the ATPase Prp5 and branch region-U2 snRNA pairing modulates the fidelity of spliceosome assembly",
      "volume": "28",
      "author": "Xu",
      "year": "2007",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib56",
      "doi-asserted-by": "crossref",
      "first-page": "376",
      "DOI": "10.1038/sj.emboj.7600050",
      "article-title": "Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA",
      "volume": "23",
      "author": "Xu",
      "year": "2004",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib57",
      "doi-asserted-by": "crossref",
      "first-page": "5000",
      "DOI": "10.1128/MCB.18.9.5000",
      "article-title": "CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif",
      "volume": "18",
      "author": "Yan",
      "year": "1998",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "issue": "12B",
      "key": "10.1016/j.molcel.2014.01.017_bib58",
      "doi-asserted-by": "crossref",
      "first-page": "2521",
      "DOI": "10.1101/gad.5.12b.2521",
      "article-title": "Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural element",
      "volume": "5",
      "author": "Zavanelli",
      "year": "1991",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2014.01.017_bib59",
      "doi-asserted-by": "crossref",
      "first-page": "605",
      "DOI": "10.1126/science.274.5287.605",
      "article-title": "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat",
      "volume": "274",
      "author": "Zhou",
      "year": "1996",
      "journal-title": "Science"
    }
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