DOI: 10.1016/j.molcel.2005.05.024. The Role of the Transcription Bubble and TFIIB in Promoter Clearance by RNA Polymerase II

The Role of the Transcription Bubble and TFIIB in Promoter Clearance by RNA Polymerase II

10.1016/j.molcel.2005.05.024

Crossref journal-article

Elsevier BV

Molecular Cell (78)

Bibliography

Pal, M., Ponticelli, A. S., & Luse, D. S. (2005). The Role of the Transcription Bubble and TFIIB in Promoter Clearance by RNA Polymerase II. Molecular Cell, 19(1), 101â110.

Authors 3

Mahadeb Pal (first)
Alfred S. Ponticelli (additional)
Donal S. Luse (additional)

References 44 Referenced 120

10.1073/pnas.1030608100 / Proc. Natl. Acad. Sci. USA / Architecture of initiation-competent 12-subunit RNA polymerase II by Armache (2003)
10.1038/nsmb1104-1031 / Nat. Struct. Mol. Biol. / Another piece in the transcription initiation puzzle by Asturias (2004)
10.1074/jbc.274.33.23203 / J. Biol. Chem. / An interaction between the N-terminal region and the core domain of yeast TFIIB promotes the formation of TATA-binding protein-TFIIB-DNA complexes by Bangur (1999)
10.1093/emboj/20.23.6826 / EMBO J. / T7 promoter release mediated by DNA scrunching by Brieba (2001)
10.1016/S0955-0674(00)00095-8 / Curr. Opin. Cell Biol. / Snapshots of RNA polymerase II transcription initiation by Buratowski (2000)
10.1073/pnas.1130601100 / Proc. Natl. Acad. Sci. USA / Complete, 12-subunit RNA polymerase II at 4.1-Å resolution: implications for the initiation of transcription by Bushnell (2003)
10.1126/science.1090838 / Science / Structural basis of transcription: An RNA polymerase II-TFIIB cocrystal at 4.5 angstroms by Bushnell (2004)
10.1016/j.cell.2004.09.028 / Cell / Mapping the location of TFIIB within the RNA polymerase II transcription preinitiation complex: A model for the structure of PIC by Chen (2004)
10.1126/science.7824954 / Science / Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators by Chiang (1995)
10.1016/S1097-2765(03)00387-3 / Mol. Cell / RNA polymerase II/TFIIF structure and conserved organization of the initiation complex by Chung (2003)
10.1128/MCB.20.21.8168-8177.2000 / Mol. Cell. Biol. / Mechanism of promoter melting by the xeroderma pigmentosum complementation group B helicase of transcription factor IIH revealed by protein-DNA photo-cross-linking by Douziech (2000)
10.1016/S0167-4781(02)00453-0 / Biochim. Biophys. Acta / Promoter escape by RNA polymerase II by Dvir (2002)
10.1074/jbc.271.13.7245 / J. Biol. Chem. / A role for ATP and TFIIH in activation of the RNA polymerase II preinitiation complex prior to transcription initiation by Dvir (1996)
10.1073/pnas.94.17.9006 / Proc. Natl. Acad. Sci. USA / A role for TFIIH in controlling the activity of early RNA polymerase II elongation complexes by Dvir (1997)
10.1016/S0959-437X(00)00181-7 / Curr. Opin. Genet. Dev. / Mechanism of transcription initiation and promoter escape by RNA polymerase II by Dvir (2001)
10.1128/MCB.21.14.4427-4440.2001 / Mol. Cell. Biol. / Promoter-specific shifts in transcription initiation conferred by yeast TFIIB mutations are determined by the sequence in the immediate vicinity of the start sites by Faitar (2001)
10.1128/MCB.24.3.1122-1131.2004 / Mol. Cell. Biol. / Photo-cross-linking of a purified preinitiation complex reveals central roles for the RNA polymerase II mobile clamp and TFIIE in initiation mechanisms by Forget (2004)
10.1126/science.8342041 / Science / DNA melting on yeast RNA polymerase-II promoters by Giardina (1993)
10.1126/science.1059495 / Science / Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 Å resolution by Gnatt (2001)
10.1038/nsmb763 / Nat. Struct. Mol. Biol. / Structure and mechanism of the RNA polymerase II transcription machinery by Hahn (2004)
10.1093/emboj/16.24.7468 / EMBO J. / Three transitions in the RNA polymerase II transcription complex during initiation by Holstege (1997)
10.1074/jbc.274.17.11526 / J. Biol. Chem. / Initially transcribed sequences strongly affect the extent of abortive initiation by RNA polymerase II by Keene (1999)
10.1016/j.molcel.2004.11.040 / Mol. Cell / Complete RNA polymerase II elongation complex structure and its interaction with NTPs and TFIIS by Kettenberger (2004)
10.1126/science.288.5470.1418 / Science / Mechanism of ATP-dependent promoter melting by transcription factor IIH by Kim (2000)
10.1074/jbc.275.9.6530 / J. Biol. Chem. / The 8-nucleotide-long RNA:DNA hybrid is a primary stability determinant of the RNA polymerase II elongation complex by Kireeva (2000)
10.1073/pnas.95.17.9767 / Proc. Natl. Acad. Sci. USA / Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event by Kumar (1998)
10.1016/S0092-8674(01)00223-9 / Cell / Defective interplay of activators and repressors with TFIIH in xeroderma pigmentosum by Liu (2001)
10.1016/S0076-6879(96)74009-0 / Methods Enzymol. / Purification of human RNA polymerase II and general transcription factors by Maldonado (1996)
10.1126/science.1069594 / Science / Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 Å resolution by Murakami (2002)
10.1128/MCB.22.1.30-40.2002 / Mol. Cell. Biol. / Strong natural pausing by RNA polymerase II within 10 bases of transcription start may result in repeated slippage and reextension of the nascent RNA by Pal (2002)
10.1073/pnas.1037057100 / Proc. Natl. Acad. Sci. USA / The initiation-elongation transition: Lateral mobility of RNA in RNA polymerase II complexes is greatly reduced at+8/+9 and absent by+23 by Pal (2003)
10.1128/MCB.21.17.5815-5825.2001 / Mol. Cell. Biol. / Promoter clearance by RNA polymerase II is an extended, multistep process strongly affected by sequence by Pal (2001)
10.1016/S0021-9258(18)43780-5 / J. Biol. Chem. / Initiation of transcription by RNA polymerase II is limited by melting of the promoter DNA in the region immediately upstream of the initiation site by Pan (1994)
10.1016/S0021-9258(18)43851-3 / J. Biol. Chem. / Characterization of sua7 mutations defines a domain of TFIIB involved in transcription start site selection in yeast by Pinto (1994)
10.1073/pnas.0307241101 / Proc. Natl. Acad. Sci. USA / Promoter unwinding and promoter clearance by RNA polymerase: detection by single-molecule DNA nanomanipulation by Revyakin (2004)
10.1146/annurev.biochem.72.121801.161520 / Annu. Rev. Biochem. / The RNA polymerase II core promoter by Smale (2003)
10.1073/pnas.101004498 / Proc. Natl. Acad. Sci. USA / TFIIH action in transcription initiation and promoter escape requires distinct regions of downstream promoter DNA by Spangler (2001)
10.1016/S0021-9258(17)32452-3 / J. Biol. Chem. / A heteroduplex template circumvents the energetic requirement for ATP during activated transcription by RNA polymerase II by Tantin (1994)
10.1038/nature752 / Nature / Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 Å resolution by Vassylyev (2002)
10.1006/prep.1993.1027 / Protein Expr. Purif. / Production of human RAP30 and RAP74 in bacterial cells by Wang (1993)
10.1126/science.1090839 / Science / Structural basis of transcription: separation of RNA from DNA by RNA polymerase II by Westover (2004)
10.1074/jbc.273.20.12492 / J. Biol. Chem. / Properties of PC4 and an RNA polymerase II complex in directing activated and basal transcription in vitro by Wu (1998)
10.1093/emboj/16.24.7457 / EMBO J. / Multiple ATP-dependent steps in RNA polymerase II promoter melting and initiation by Yan (1997)
10.1074/jbc.274.49.34819 / J. Biol. Chem. / The use of ATP and initiating nucleotides during postrecruitment steps at the activated adenovirus E4 promoter by Yan (1999)

Dates

Type	When
Created	20 years, 1 month ago (July 1, 2005, 9:15 a.m.)
Deposited	4 years, 1 month ago (July 12, 2021, 4:31 p.m.)
Indexed	3 months, 2 weeks ago (May 6, 2025, 3:46 a.m.)
Issued	20 years, 1 month ago (July 1, 2005)
Published	20 years, 1 month ago (July 1, 2005)
Published Print	20 years, 1 month ago (July 1, 2005)

Funders 0

None

BibTeX

@article{Pal_2005, title={The Role of the Transcription Bubble and TFIIB in Promoter Clearance by RNA Polymerase II}, volume={19}, ISSN={1097-2765}, url={http://dx.doi.org/10.1016/j.molcel.2005.05.024}, DOI={10.1016/j.molcel.2005.05.024}, number={1}, journal={Molecular Cell}, publisher={Elsevier BV}, author={Pal, Mahadeb and Ponticelli, Alfred S. and Luse, Donal S.}, year={2005}, month=jul, pages={101–110} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        6
      ]
    ],
    "date-time": "2025-05-06T07:46:46Z",
    "timestamp": 1746517606509
  },
  "reference-count": 44,
  "publisher": "Elsevier BV",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            7,
            1
          ]
        ],
        "date-time": "2005-07-01T00:00:00Z",
        "timestamp": 1120176000000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    },
    {
      "start": {
        "date-parts": [
          [
            2013,
            7,
            17
          ]
        ],
        "date-time": "2013-07-17T00:00:00Z",
        "timestamp": 1374019200000
      },
      "content-version": "vor",
      "delay-in-days": 2938,
      "URL": "https://www.elsevier.com/open-access/userlicense/1.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2005,
        7
      ]
    ]
  },
  "DOI": "10.1016/j.molcel.2005.05.024",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        7,
        1
      ]
    ],
    "date-time": "2005-07-01T13:15:18Z",
    "timestamp": 1120223718000
  },
  "page": "101-110",
  "source": "Crossref",
  "is-referenced-by-count": 120,
  "title": "The Role of the Transcription Bubble and TFIIB in Promoter Clearance by RNA Polymerase II",
  "prefix": "10.1016",
  "volume": "19",
  "author": [
    {
      "given": "Mahadeb",
      "family": "Pal",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Alfred S.",
      "family": "Ponticelli",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Donal S.",
      "family": "Luse",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/j.molcel.2005.05.024_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "6964",
      "DOI": "10.1073/pnas.1030608100",
      "article-title": "Architecture of initiation-competent 12-subunit RNA polymerase II",
      "volume": "100",
      "author": "Armache",
      "year": "2003",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "1031",
      "DOI": "10.1038/nsmb1104-1031",
      "article-title": "Another piece in the transcription initiation puzzle",
      "volume": "11",
      "author": "Asturias",
      "year": "2004",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "23203",
      "DOI": "10.1074/jbc.274.33.23203",
      "article-title": "An interaction between the N-terminal region and the core domain of yeast TFIIB promotes the formation of TATA-binding protein-TFIIB-DNA complexes",
      "volume": "274",
      "author": "Bangur",
      "year": "1999",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "6826",
      "DOI": "10.1093/emboj/20.23.6826",
      "article-title": "T7 promoter release mediated by DNA scrunching",
      "volume": "20",
      "author": "Brieba",
      "year": "2001",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "320",
      "DOI": "10.1016/S0955-0674(00)00095-8",
      "article-title": "Snapshots of RNA polymerase II transcription initiation",
      "volume": "12",
      "author": "Buratowski",
      "year": "2000",
      "journal-title": "Curr. Opin. Cell Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "6969",
      "DOI": "10.1073/pnas.1130601100",
      "article-title": "Complete, 12-subunit RNA polymerase II at 4.1-\u00c5 resolution: implications for the initiation of transcription",
      "volume": "100",
      "author": "Bushnell",
      "year": "2003",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "983",
      "DOI": "10.1126/science.1090838",
      "article-title": "Structural basis of transcription: An RNA polymerase II-TFIIB cocrystal at 4.5 angstroms",
      "volume": "303",
      "author": "Bushnell",
      "year": "2004",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "169",
      "DOI": "10.1016/j.cell.2004.09.028",
      "article-title": "Mapping the location of TFIIB within the RNA polymerase II transcription preinitiation complex: A model for the structure of PIC",
      "volume": "119",
      "author": "Chen",
      "year": "2004",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "531",
      "DOI": "10.1126/science.7824954",
      "article-title": "Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators",
      "volume": "267",
      "author": "Chiang",
      "year": "1995",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "1003",
      "DOI": "10.1016/S1097-2765(03)00387-3",
      "article-title": "RNA polymerase II/TFIIF structure and conserved organization of the initiation complex",
      "volume": "12",
      "author": "Chung",
      "year": "2003",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "8168",
      "DOI": "10.1128/MCB.20.21.8168-8177.2000",
      "article-title": "Mechanism of promoter melting by the xeroderma pigmentosum complementation group B helicase of transcription factor IIH revealed by protein-DNA photo-cross-linking",
      "volume": "20",
      "author": "Douziech",
      "year": "2000",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "208",
      "DOI": "10.1016/S0167-4781(02)00453-0",
      "article-title": "Promoter escape by RNA polymerase II",
      "volume": "1577",
      "author": "Dvir",
      "year": "2002",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "7245",
      "DOI": "10.1074/jbc.271.13.7245",
      "article-title": "A role for ATP and TFIIH in activation of the RNA polymerase II preinitiation complex prior to transcription initiation",
      "volume": "271",
      "author": "Dvir",
      "year": "1996",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "9006",
      "DOI": "10.1073/pnas.94.17.9006",
      "article-title": "A role for TFIIH in controlling the activity of early RNA polymerase II elongation complexes",
      "volume": "94",
      "author": "Dvir",
      "year": "1997",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "209",
      "DOI": "10.1016/S0959-437X(00)00181-7",
      "article-title": "Mechanism of transcription initiation and promoter escape by RNA polymerase II",
      "volume": "11",
      "author": "Dvir",
      "year": "2001",
      "journal-title": "Curr. Opin. Genet. Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "4427",
      "DOI": "10.1128/MCB.21.14.4427-4440.2001",
      "article-title": "Promoter-specific shifts in transcription initiation conferred by yeast TFIIB mutations are determined by the sequence in the immediate vicinity of the start sites",
      "volume": "21",
      "author": "Faitar",
      "year": "2001",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "1122",
      "DOI": "10.1128/MCB.24.3.1122-1131.2004",
      "article-title": "Photo-cross-linking of a purified preinitiation complex reveals central roles for the RNA polymerase II mobile clamp and TFIIE in initiation mechanisms",
      "volume": "24",
      "author": "Forget",
      "year": "2004",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "759",
      "DOI": "10.1126/science.8342041",
      "article-title": "DNA melting on yeast RNA polymerase-II promoters",
      "volume": "261",
      "author": "Giardina",
      "year": "1993",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib19",
      "doi-asserted-by": "crossref",
      "first-page": "1876",
      "DOI": "10.1126/science.1059495",
      "article-title": "Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 \u00c5 resolution",
      "volume": "292",
      "author": "Gnatt",
      "year": "2001",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib20",
      "doi-asserted-by": "crossref",
      "first-page": "394",
      "DOI": "10.1038/nsmb763",
      "article-title": "Structure and mechanism of the RNA polymerase II transcription machinery",
      "volume": "11",
      "author": "Hahn",
      "year": "2004",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "7468",
      "DOI": "10.1093/emboj/16.24.7468",
      "article-title": "Three transitions in the RNA polymerase II transcription complex during initiation",
      "volume": "16",
      "author": "Holstege",
      "year": "1997",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "11526",
      "DOI": "10.1074/jbc.274.17.11526",
      "article-title": "Initially transcribed sequences strongly affect the extent of abortive initiation by RNA polymerase II",
      "volume": "274",
      "author": "Keene",
      "year": "1999",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "955",
      "DOI": "10.1016/j.molcel.2004.11.040",
      "article-title": "Complete RNA polymerase II elongation complex structure and its interaction with NTPs and TFIIS",
      "volume": "16",
      "author": "Kettenberger",
      "year": "2004",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "1418",
      "DOI": "10.1126/science.288.5470.1418",
      "article-title": "Mechanism of ATP-dependent promoter melting by transcription factor IIH",
      "volume": "288",
      "author": "Kim",
      "year": "2000",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "6530",
      "DOI": "10.1074/jbc.275.9.6530",
      "article-title": "The 8-nucleotide-long RNA:DNA hybrid is a primary stability determinant of the RNA polymerase II elongation complex",
      "volume": "275",
      "author": "Kireeva",
      "year": "2000",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "9767",
      "DOI": "10.1073/pnas.95.17.9767",
      "article-title": "Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event",
      "volume": "95",
      "author": "Kumar",
      "year": "1998",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "353",
      "DOI": "10.1016/S0092-8674(01)00223-9",
      "article-title": "Defective interplay of activators and repressors with TFIIH in xeroderma pigmentosum",
      "volume": "104",
      "author": "Liu",
      "year": "2001",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "72",
      "DOI": "10.1016/S0076-6879(96)74009-0",
      "article-title": "Purification of human RNA polymerase II and general transcription factors",
      "volume": "274",
      "author": "Maldonado",
      "year": "1996",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib29",
      "doi-asserted-by": "crossref",
      "first-page": "1280",
      "DOI": "10.1126/science.1069594",
      "article-title": "Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 \u00c5 resolution",
      "volume": "296",
      "author": "Murakami",
      "year": "2002",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "30",
      "DOI": "10.1128/MCB.22.1.30-40.2002",
      "article-title": "Strong natural pausing by RNA polymerase II within 10 bases of transcription start may result in repeated slippage and reextension of the nascent RNA",
      "volume": "22",
      "author": "Pal",
      "year": "2002",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "5700",
      "DOI": "10.1073/pnas.1037057100",
      "article-title": "The initiation-elongation transition: Lateral mobility of RNA in RNA polymerase II complexes is greatly reduced at+8/+9 and absent by+23",
      "volume": "100",
      "author": "Pal",
      "year": "2003",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib32",
      "doi-asserted-by": "crossref",
      "first-page": "5815",
      "DOI": "10.1128/MCB.21.17.5815-5825.2001",
      "article-title": "Promoter clearance by RNA polymerase II is an extended, multistep process strongly affected by sequence",
      "volume": "21",
      "author": "Pal",
      "year": "2001",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "30101",
      "DOI": "10.1016/S0021-9258(18)43780-5",
      "article-title": "Initiation of transcription by RNA polymerase II is limited by melting of the promoter DNA in the region immediately upstream of the initiation site",
      "volume": "269",
      "author": "Pan",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "30569",
      "DOI": "10.1016/S0021-9258(18)43851-3",
      "article-title": "Characterization of sua7 mutations defines a domain of TFIIB involved in transcription start site selection in yeast",
      "volume": "269",
      "author": "Pinto",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "4776",
      "DOI": "10.1073/pnas.0307241101",
      "article-title": "Promoter unwinding and promoter clearance by RNA polymerase: detection by single-molecule DNA nanomanipulation",
      "volume": "101",
      "author": "Revyakin",
      "year": "2004",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib36",
      "doi-asserted-by": "crossref",
      "first-page": "449",
      "DOI": "10.1146/annurev.biochem.72.121801.161520",
      "article-title": "The RNA polymerase II core promoter",
      "volume": "72",
      "author": "Smale",
      "year": "2003",
      "journal-title": "Annu. Rev. Biochem."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib37",
      "doi-asserted-by": "crossref",
      "first-page": "5544",
      "DOI": "10.1073/pnas.101004498",
      "article-title": "TFIIH action in transcription initiation and promoter escape requires distinct regions of downstream promoter DNA",
      "volume": "98",
      "author": "Spangler",
      "year": "2001",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib38",
      "doi-asserted-by": "crossref",
      "first-page": "17397",
      "DOI": "10.1016/S0021-9258(17)32452-3",
      "article-title": "A heteroduplex template circumvents the energetic requirement for ATP during activated transcription by RNA polymerase II",
      "volume": "269",
      "author": "Tantin",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib39",
      "doi-asserted-by": "crossref",
      "first-page": "712",
      "DOI": "10.1038/nature752",
      "article-title": "Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 \u00c5 resolution",
      "volume": "417",
      "author": "Vassylyev",
      "year": "2002",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib40",
      "doi-asserted-by": "crossref",
      "first-page": "207",
      "DOI": "10.1006/prep.1993.1027",
      "article-title": "Production of human RAP30 and RAP74 in bacterial cells",
      "volume": "4",
      "author": "Wang",
      "year": "1993",
      "journal-title": "Protein Expr. Purif."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib41",
      "doi-asserted-by": "crossref",
      "first-page": "1014",
      "DOI": "10.1126/science.1090839",
      "article-title": "Structural basis of transcription: separation of RNA from DNA by RNA polymerase II",
      "volume": "303",
      "author": "Westover",
      "year": "2004",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib42",
      "doi-asserted-by": "crossref",
      "first-page": "12492",
      "DOI": "10.1074/jbc.273.20.12492",
      "article-title": "Properties of PC4 and an RNA polymerase II complex in directing activated and basal transcription in vitro",
      "volume": "273",
      "author": "Wu",
      "year": "1998",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib43",
      "doi-asserted-by": "crossref",
      "first-page": "7457",
      "DOI": "10.1093/emboj/16.24.7457",
      "article-title": "Multiple ATP-dependent steps in RNA polymerase II promoter melting and initiation",
      "volume": "16",
      "author": "Yan",
      "year": "1997",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.024_bib44",
      "doi-asserted-by": "crossref",
      "first-page": "34819",
      "DOI": "10.1074/jbc.274.49.34819",
      "article-title": "The use of ATP and initiating nucleotides during postrecruitment steps at the activated adenovirus E4 promoter",
      "volume": "274",
      "author": "Yan",
      "year": "1999",
      "journal-title": "J. Biol. Chem."
    }
  ],
  "container-title": "Molecular Cell",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S109727650501350X?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S109727650501350X?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        12
      ]
    ],
    "date-time": "2021-07-12T20:31:08Z",
    "timestamp": 1626121868000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S109727650501350X"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2005,
        7
      ]
    ]
  },
  "references-count": 44,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          2005,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "S109727650501350X"
  ],
  "URL": "http://dx.doi.org/10.1016/j.molcel.2005.05.024",
  "relation": {},
  "ISSN": [
    "1097-2765"
  ],
  "subject": [],
  "container-title-short": "Molecular Cell",
  "published": {
    "date-parts": [
      [
        2005,
        7
      ]
    ]
  }
}