DOI: 10.1016/j.molcel.2005.05.006. Cotranscriptional Spliceosome Assembly Dynamics and the Role of U1 snRNA:5′ss Base Pairing in Yeast

Cotranscriptional Spliceosome Assembly Dynamics and the Role of U1 snRNA:5′ss Base Pairing in Yeast

10.1016/j.molcel.2005.05.006

Crossref journal-article

Elsevier BV

Molecular Cell (78)

Authors 2

Scott A. Lacadie (first)
Michael Rosbash (additional)

References 71 Referenced 155

10.1016/S0092-8674(00)80221-4 / Cell / Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals by Abovich (1997)
10.1101/gad.8.7.843 / Genes Dev. / The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition by Abovich (1994)
10.1038/sj.emboj.7600261 / EMBO J. / Biochemical analysis of TREX complex recruitment to intronless and intron-containing yeast genes by Abruzzi (2004)
10.1038/342819a0 / Nature / Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein by Anderson (1989)
10.1017/S1355838299991379 / RNA / A handful of intron-containing genes produces the lion's share of yeast mRNA by Ares (1999)
10.1016/0092-8674(94)90182-1 / Cell / Splicing of balbiani ring 1 gene pre-mRNA occurs simultaneously with transcription by Bauren (1994)
10.1016/S0092-8674(00)80261-5 / Cell / The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC by Berglund (1997)
10.1017/S1355838201002187 / RNA / Crystal structure of a model branchpoint-U2 snRNA duplex containing bulged adenosines by Berglund (2001)
10.1101/gad.2.6.754 / Genes Dev. / Splice site selection, rate of splicing, and alternative splicing on nascent transcripts by Beyer (1988)
{'key': '10.1016/j.molcel.2005.05.006_bib10', 'series-title': 'The RNA World', 'first-page': '525', 'article-title': 'Splicing of precursors to mRNAs by the spliceosomes', 'author': 'Burge', 'year': '1999'} / The RNA World / Splicing of precursors to mRNAs by the spliceosomes by Burge (1999)
10.1093/emboj/17.21.6348 / EMBO J. / The yeast U2A′/U2B complex is required for pre-spliceosome formation by Caspary (1998)
10.1016/S1097-2765(01)00170-8 / Mol. Cell / Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor by Chen (2001)
10.1101/gad.10.13.1699 / Genes Dev. / The yeast splicing factor MUD13 is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex by Colot (1996)
10.1038/nature00947 / Nature / The U1 snRNP protein U1C recognizes the 5′ splice site in the absence of base pairing by Du (2002)
10.1073/pnas.0406319101 / Proc. Natl. Acad. Sci. USA / Effects of the U1C L13 mutation and temperature regulation of yeast commitment complex formation by Du (2004)
10.1101/gad.983602 / Genes Dev. / Promoter proximal splice sites enhance transcription by Furger (2002)
10.1016/S0092-8674(03)01026-2 / Cell / Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1 by Galy (2004)
Görnemann, J., Kotovic, K.M., Hujer, K., and Neugebauer, K.M. (2005). Cotranscriptional spliceosome assembly occurs in a stepwise fashion and requires the cap binding complex. Mol. Cell 19, this issue, 53–63. (10.1016/j.molcel.2005.05.007)
10.1073/pnas.79.23.7410 / Proc. Natl. Acad. Sci. USA / A GAL10–CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site by Guarente (1982)
{'key': '10.1016/j.molcel.2005.05.006_bib20', 'first-page': '389', 'article-title': 'Guide to yeast genetics and molecular biology', 'volume': '194', 'author': 'Guthrie', 'year': '1991', 'journal-title': 'Methods Enzymol.'} / Methods Enzymol. / Guide to yeast genetics and molecular biology by Guthrie (1991)
10.1073/pnas.83.16.5835 / Proc. Natl. Acad. Sci. USA / RNA splicing and intron turnover are greatly diminished by a mutant yeast branch point by Jacquier (1986)
10.1016/0092-8674(85)90172-2 / Cell / A quantitative analysis of the effects of 5′ junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing by Jacquier (1985)
10.1016/S1097-2765(03)00191-6 / Mol. Cell / Early formation of mRNP: license for export or quality control? by Jensen (2003)
10.1016/S1097-2765(03)00270-3 / Mol. Cell / Pre-mRNA splicing: awash in a sea of proteins by Jurica (2003)
10.1126/science.8266100 / Science / Involvement of U6 snRNA in 5′ splice site selection by Kandels-Lewis (1993)
10.1002/j.1460-2075.1994.tb06952.x / EMBO J. / Assembly and disassembly of spliceosomes along a specific pre-messenger RNP fiber by Kiseleva (1994)
10.1016/0092-8674(93)90531-T / Cell / Disruption of base pairing between the 5′ splice site and the 5′ end of U1 snRNA is required for spliceosome assembly by Konforti (1993)
10.1261/rna.7100104 / RNA / Multiple links between transcription and splicing by Kornblihtt (2004)
10.1128/MCB.23.16.5768-5779.2003 / Mol. Cell. Biol. / Cotranscriptional recruitment of the U1 snRNP to intron-containing genes in yeast by Kotovic (2003)
10.1016/0092-8674(89)90127-X / Cell / Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm by Legrain (1989)
10.1101/gad.1032902 / Genes Dev. / Intron status and 3′-end formation control cotranscriptional export of mRNA by Lei (2002)
10.1128/MCB.10.11.6059 / Mol. Cell. Biol. / Splicing precedes polyadenylation during Drosophila E74A transcription by LeMaire (1990)
10.1126/science.8266093 / Science / Mutations in U6 snRNA that alter splice site specificity: implications for the active site by Lesser (1993)
10.1101/gad.10.13.1683 / Genes Dev. / A nuclear cap-binding complex facilitates association of U1 snRNP with the cap-proximal 5′ splice site by Lewis (1996)
10.1093/nar/20.16.4237 / Nucleic Acids Res. / Requirements for U2 snRNP addition to yeast pre-mRNA by Liao (1992)
10.1101/gad.852101 / Genes Dev. / Multiple roles for the yeast SUB2/yUAP56 gene in splicing by Libri (2001)
10.1101/gad.8.24.3008 / Genes Dev. / Dynamic association of proteins with the pre-mRNA branch region by MacMillan (1994)
10.1016/0092-8674(92)90556-R / Cell / A novel base-pairing interaction between U2 and U6 mRNAs suggests a mechanism for the catalytic activation of the spliceosome by Madhani (1992)
10.1016/S1097-2765(00)00032-0 / Mol. Cell / Functional recognition of 5′ splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly by Maroney (2000)
10.1101/gad.5.12b.2534 / Genes Dev. / An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway by Michaud (1991)
10.1242/jcs.00073 / J. Cell Sci. / On the importance of being co-transcriptional by Neugebauer (2002)
10.1016/0092-8674(91)90413-S / Cell / Mutations in yeast U5 snRNA alter the specificity of 5′ splice-site cleavage by Newman (1991)
10.1093/emboj/16.19.5797 / EMBO J. / The role of U5 snRNP in pre-mRNA splicing by Newman (1997)
10.1016/0092-8674(92)90149-7 / Cell / U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites by Newman (1992)
10.1016/0092-8674(87)90564-2 / Cell / Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA by Parker (1987)
10.1128/MCB.17.7.3469 / Mol. Cell. Biol. / The branchpoint residue is recognized during commitment complex formation before being bulged out of the U2 snRNA-pre-mRNA duplex by Pascolo (1997)
10.1016/0092-8674(85)90066-2 / Cell / mRNA splicing efficiency in yeast and the contribution of nonconserved sequences by Pikielny (1985)
10.1101/gad.13.5.569 / Genes Dev. / Interaction of the U1 snRNP with nonconserved intronic sequences affects 5′ splice site selection by Puig (1999)
10.1101/gad.8.5.587 / Genes Dev. / Branch nucleophile selection in pre-mRNA splicing: evidence for the bulged duplex model by Query (1994)
{'key': '10.1016/j.molcel.2005.05.006_bib50', 'first-page': '213', 'article-title': 'The canonical GU dinucleotide at the 5′ splice site is recognized by p220 of the U5 snRNP within the spliceosome', 'volume': '2', 'author': 'Reyes', 'year': '1996', 'journal-title': 'RNA'} / RNA / The canonical GU dinucleotide at the 5′ splice site is recognized by p220 of the U5 snRNP within the spliceosome by Reyes (1996)
10.1101/gad.7.10.1909 / Genes Dev. / Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA by Ruby (1993)
10.1017/S1355838299982286 / RNA / Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly by Rutz (1999)
10.1093/emboj/19.8.1873 / EMBO J. / A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing by Rutz (2000)
10.1073/pnas.89.23.11269 / Proc. Natl. Acad. Sci. USA / Evidence for a base-pairing interaction between U6 snRNA and the 5′ splice site during the splicing reaction in yeast by Sawa (1992)
10.1016/0092-8674(89)90296-1 / Cell / Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing by Seraphin (1989)
10.1002/j.1460-2075.1991.tb08062.x / EMBO J. / The yeast branchpoint sequence is not required for the formation of a stable U1 snRNP-pre-mRNA complex and is recognized in the absence of U2 snRNA by Seraphin (1991)
10.1002/j.1460-2075.1988.tb03101.x / EMBO J. / A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site by Seraphin (1988)
10.1017/S1355838299981682 / RNA / Genome-wide bioinformatic and molecular analysis of introns in Saccharomyces cerevisiae by Spingola (1999)
10.1016/S1097-2765(00)80174-4 / Mol. Cell / An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p by Staley (1999)
10.1016/S1097-2765(02)00436-7 / Mol. Cell / Composition and functional characterization of the yeast spliceosomal penta-snRNP by Stevens (2002)
10.1093/emboj/16.13.4082 / EMBO J. / Identification and characterization of the yeast homologue of U1 snRNP-specific protein C by Tang (1997)
10.1073/pnas.80.14.4403 / Proc. Natl. Acad. Sci. USA / Expression of a β-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast by Teem (1983)
10.1002/j.1460-2075.1995.tb07258.x / EMBO J. / Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA by Teigelkamp (1995)
{'key': '10.1016/j.molcel.2005.05.006_bib64', 'first-page': '584', 'article-title': 'Prp16p, Slu7p, and Prp8p interact with the 3′ splice site in two distinct stages during the second catalytic step of pre-mRNA splicing', 'volume': '1', 'author': 'Umen', 'year': '1995', 'journal-title': 'RNA'} / RNA / Prp16p, Slu7p, and Prp8p interact with the 3′ splice site in two distinct stages during the second catalytic step of pre-mRNA splicing by Umen (1995)
10.1016/S0092-8674(02)00726-2 / Cell / Spliceosomal snRNAs: Mg(2+)-dependent chemistry at the catalytic core? by Villa (2002)
10.1126/science.1411506 / Science / Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing by Wassarman (1992)
10.1128/MCB.14.11.7219 / Mol. Cell. Biol. / Physical isolation of nascent RNA chains transcribed by RNA polymerase II: evidence for cotranscriptional splicing by Wuarin (1994)
10.1101/gad.6.12b.2542 / Genes Dev. / Site-specific cross-linking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing by Wyatt (1992)
10.1101/gad.13.5.581 / Genes Dev. / Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex by Zhang (1999)
10.1016/S1097-2765(01)00180-0 / Mol. Cell / A biochemical function for the Sm complex by Zhang (2001)
10.1101/gad.851701 / Genes Dev. / Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56 by Zhang (2001)

Dates

Type	When
Created	20 years, 1 month ago (July 1, 2005, 9:15 a.m.)
Deposited	6 years, 5 months ago (March 3, 2019, 4:24 p.m.)
Indexed	1 month, 3 weeks ago (July 5, 2025, 8:33 p.m.)
Issued	20 years, 1 month ago (July 1, 2005)
Published	20 years, 1 month ago (July 1, 2005)
Published Print	20 years, 1 month ago (July 1, 2005)

Funders 0

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BibTeX

@article{Lacadie_2005, title={Cotranscriptional Spliceosome Assembly Dynamics and the Role of U1 snRNA:5′ss Base Pairing in Yeast}, volume={19}, ISSN={1097-2765}, url={http://dx.doi.org/10.1016/j.molcel.2005.05.006}, DOI={10.1016/j.molcel.2005.05.006}, number={1}, journal={Molecular Cell}, publisher={Elsevier BV}, author={Lacadie, Scott A. and Rosbash, Michael}, year={2005}, month=jul, pages={65–75} }

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      "author": "Ares",
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      "author": "Beyer",
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      "author": "Guarente",
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      "author": "Guthrie",
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      "volume": "83",
      "author": "Jacquier",
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      "article-title": "A quantitative analysis of the effects of 5\u2032 junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing",
      "volume": "43",
      "author": "Jacquier",
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      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "1129",
      "DOI": "10.1016/S1097-2765(03)00191-6",
      "article-title": "Early formation of mRNP: license for export or quality control?",
      "volume": "11",
      "author": "Jensen",
      "year": "2003",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "5",
      "DOI": "10.1016/S1097-2765(03)00270-3",
      "article-title": "Pre-mRNA splicing: awash in a sea of proteins",
      "volume": "12",
      "author": "Jurica",
      "year": "2003",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "2035",
      "DOI": "10.1126/science.8266100",
      "article-title": "Involvement of U6 snRNA in 5\u2032 splice site selection",
      "volume": "262",
      "author": "Kandels-Lewis",
      "year": "1993",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "6052",
      "DOI": "10.1002/j.1460-2075.1994.tb06952.x",
      "article-title": "Assembly and disassembly of spliceosomes along a specific pre-messenger RNP fiber",
      "volume": "13",
      "author": "Kiseleva",
      "year": "1994",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "863",
      "DOI": "10.1016/0092-8674(93)90531-T",
      "article-title": "Disruption of base pairing between the 5\u2032 splice site and the 5\u2032 end of U1 snRNA is required for spliceosome assembly",
      "volume": "75",
      "author": "Konforti",
      "year": "1993",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "1489",
      "DOI": "10.1261/rna.7100104",
      "article-title": "Multiple links between transcription and splicing",
      "volume": "10",
      "author": "Kornblihtt",
      "year": "2004",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib29",
      "doi-asserted-by": "crossref",
      "first-page": "5768",
      "DOI": "10.1128/MCB.23.16.5768-5779.2003",
      "article-title": "Cotranscriptional recruitment of the U1 snRNP to intron-containing genes in yeast",
      "volume": "23",
      "author": "Kotovic",
      "year": "2003",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "573",
      "DOI": "10.1016/0092-8674(89)90127-X",
      "article-title": "Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm",
      "volume": "57",
      "author": "Legrain",
      "year": "1989",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "2761",
      "DOI": "10.1101/gad.1032902",
      "article-title": "Intron status and 3\u2032-end formation control cotranscriptional export of mRNA",
      "volume": "16",
      "author": "Lei",
      "year": "2002",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib32",
      "doi-asserted-by": "crossref",
      "first-page": "6059",
      "DOI": "10.1128/MCB.10.11.6059",
      "article-title": "Splicing precedes polyadenylation during Drosophila E74A transcription",
      "volume": "10",
      "author": "LeMaire",
      "year": "1990",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "1982",
      "DOI": "10.1126/science.8266093",
      "article-title": "Mutations in U6 snRNA that alter splice site specificity: implications for the active site",
      "volume": "262",
      "author": "Lesser",
      "year": "1993",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "1683",
      "DOI": "10.1101/gad.10.13.1683",
      "article-title": "A nuclear cap-binding complex facilitates association of U1 snRNP with the cap-proximal 5\u2032 splice site",
      "volume": "10",
      "author": "Lewis",
      "year": "1996",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "4237",
      "DOI": "10.1093/nar/20.16.4237",
      "article-title": "Requirements for U2 snRNP addition to yeast pre-mRNA",
      "volume": "20",
      "author": "Liao",
      "year": "1992",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib36",
      "doi-asserted-by": "crossref",
      "first-page": "36",
      "DOI": "10.1101/gad.852101",
      "article-title": "Multiple roles for the yeast SUB2/yUAP56 gene in splicing",
      "volume": "15",
      "author": "Libri",
      "year": "2001",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib37",
      "doi-asserted-by": "crossref",
      "first-page": "3008",
      "DOI": "10.1101/gad.8.24.3008",
      "article-title": "Dynamic association of proteins with the pre-mRNA branch region",
      "volume": "8",
      "author": "MacMillan",
      "year": "1994",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib38",
      "doi-asserted-by": "crossref",
      "first-page": "803",
      "DOI": "10.1016/0092-8674(92)90556-R",
      "article-title": "A novel base-pairing interaction between U2 and U6 mRNAs suggests a mechanism for the catalytic activation of the spliceosome",
      "volume": "71",
      "author": "Madhani",
      "year": "1992",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib39",
      "doi-asserted-by": "crossref",
      "first-page": "317",
      "DOI": "10.1016/S1097-2765(00)00032-0",
      "article-title": "Functional recognition of 5\u2032 splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly",
      "volume": "6",
      "author": "Maroney",
      "year": "2000",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib40",
      "doi-asserted-by": "crossref",
      "first-page": "2534",
      "DOI": "10.1101/gad.5.12b.2534",
      "article-title": "An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway",
      "volume": "5",
      "author": "Michaud",
      "year": "1991",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib41",
      "doi-asserted-by": "crossref",
      "first-page": "3865",
      "DOI": "10.1242/jcs.00073",
      "article-title": "On the importance of being co-transcriptional",
      "volume": "115",
      "author": "Neugebauer",
      "year": "2002",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib42",
      "doi-asserted-by": "crossref",
      "first-page": "115",
      "DOI": "10.1016/0092-8674(91)90413-S",
      "article-title": "Mutations in yeast U5 snRNA alter the specificity of 5\u2032 splice-site cleavage",
      "volume": "65",
      "author": "Newman",
      "year": "1991",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib43",
      "doi-asserted-by": "crossref",
      "first-page": "5797",
      "DOI": "10.1093/emboj/16.19.5797",
      "article-title": "The role of U5 snRNP in pre-mRNA splicing",
      "volume": "16",
      "author": "Newman",
      "year": "1997",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib44",
      "doi-asserted-by": "crossref",
      "first-page": "743",
      "DOI": "10.1016/0092-8674(92)90149-7",
      "article-title": "U5 snRNA interacts with exon sequences at 5\u2032 and 3\u2032 splice sites",
      "volume": "68",
      "author": "Newman",
      "year": "1992",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib45",
      "doi-asserted-by": "crossref",
      "first-page": "229",
      "DOI": "10.1016/0092-8674(87)90564-2",
      "article-title": "Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA",
      "volume": "49",
      "author": "Parker",
      "year": "1987",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib46",
      "doi-asserted-by": "crossref",
      "first-page": "3469",
      "DOI": "10.1128/MCB.17.7.3469",
      "article-title": "The branchpoint residue is recognized during commitment complex formation before being bulged out of the U2 snRNA-pre-mRNA duplex",
      "volume": "17",
      "author": "Pascolo",
      "year": "1997",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib47",
      "doi-asserted-by": "crossref",
      "first-page": "119",
      "DOI": "10.1016/0092-8674(85)90066-2",
      "article-title": "mRNA splicing efficiency in yeast and the contribution of nonconserved sequences",
      "volume": "41",
      "author": "Pikielny",
      "year": "1985",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib48",
      "doi-asserted-by": "crossref",
      "first-page": "569",
      "DOI": "10.1101/gad.13.5.569",
      "article-title": "Interaction of the U1 snRNP with nonconserved intronic sequences affects 5\u2032 splice site selection",
      "volume": "13",
      "author": "Puig",
      "year": "1999",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib49",
      "doi-asserted-by": "crossref",
      "first-page": "587",
      "DOI": "10.1101/gad.8.5.587",
      "article-title": "Branch nucleophile selection in pre-mRNA splicing: evidence for the bulged duplex model",
      "volume": "8",
      "author": "Query",
      "year": "1994",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib50",
      "first-page": "213",
      "article-title": "The canonical GU dinucleotide at the 5\u2032 splice site is recognized by p220 of the U5 snRNP within the spliceosome",
      "volume": "2",
      "author": "Reyes",
      "year": "1996",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib51",
      "doi-asserted-by": "crossref",
      "first-page": "1909",
      "DOI": "10.1101/gad.7.10.1909",
      "article-title": "Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA",
      "volume": "7",
      "author": "Ruby",
      "year": "1993",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib52",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1017/S1355838299982286",
      "article-title": "Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly",
      "volume": "5",
      "author": "Rutz",
      "year": "1999",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib53",
      "doi-asserted-by": "crossref",
      "first-page": "1873",
      "DOI": "10.1093/emboj/19.8.1873",
      "article-title": "A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing",
      "volume": "19",
      "author": "Rutz",
      "year": "2000",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib54",
      "doi-asserted-by": "crossref",
      "first-page": "11269",
      "DOI": "10.1073/pnas.89.23.11269",
      "article-title": "Evidence for a base-pairing interaction between U6 snRNA and the 5\u2032 splice site during the splicing reaction in yeast",
      "volume": "89",
      "author": "Sawa",
      "year": "1992",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib55",
      "doi-asserted-by": "crossref",
      "first-page": "349",
      "DOI": "10.1016/0092-8674(89)90296-1",
      "article-title": "Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing",
      "volume": "59",
      "author": "Seraphin",
      "year": "1989",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib56",
      "doi-asserted-by": "crossref",
      "first-page": "1209",
      "DOI": "10.1002/j.1460-2075.1991.tb08062.x",
      "article-title": "The yeast branchpoint sequence is not required for the formation of a stable U1 snRNP-pre-mRNA complex and is recognized in the absence of U2 snRNA",
      "volume": "10",
      "author": "Seraphin",
      "year": "1991",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib57",
      "doi-asserted-by": "crossref",
      "first-page": "2533",
      "DOI": "10.1002/j.1460-2075.1988.tb03101.x",
      "article-title": "A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5\u2032 cleavage site",
      "volume": "7",
      "author": "Seraphin",
      "year": "1988",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib58",
      "doi-asserted-by": "crossref",
      "first-page": "221",
      "DOI": "10.1017/S1355838299981682",
      "article-title": "Genome-wide bioinformatic and molecular analysis of introns in Saccharomyces cerevisiae",
      "volume": "5",
      "author": "Spingola",
      "year": "1999",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib59",
      "doi-asserted-by": "crossref",
      "first-page": "55",
      "DOI": "10.1016/S1097-2765(00)80174-4",
      "article-title": "An RNA switch at the 5\u2032 splice site requires ATP and the DEAD box protein Prp28p",
      "volume": "3",
      "author": "Staley",
      "year": "1999",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib60",
      "doi-asserted-by": "crossref",
      "first-page": "31",
      "DOI": "10.1016/S1097-2765(02)00436-7",
      "article-title": "Composition and functional characterization of the yeast spliceosomal penta-snRNP",
      "volume": "9",
      "author": "Stevens",
      "year": "2002",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib61",
      "doi-asserted-by": "crossref",
      "first-page": "4082",
      "DOI": "10.1093/emboj/16.13.4082",
      "article-title": "Identification and characterization of the yeast homologue of U1 snRNP-specific protein C",
      "volume": "16",
      "author": "Tang",
      "year": "1997",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib62",
      "doi-asserted-by": "crossref",
      "first-page": "4403",
      "DOI": "10.1073/pnas.80.14.4403",
      "article-title": "Expression of a \u03b2-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast",
      "volume": "80",
      "author": "Teem",
      "year": "1983",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib63",
      "doi-asserted-by": "crossref",
      "first-page": "2602",
      "DOI": "10.1002/j.1460-2075.1995.tb07258.x",
      "article-title": "Extensive interactions of PRP8 protein with the 5\u2032 and 3\u2032 splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA",
      "volume": "14",
      "author": "Teigelkamp",
      "year": "1995",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib64",
      "first-page": "584",
      "article-title": "Prp16p, Slu7p, and Prp8p interact with the 3\u2032 splice site in two distinct stages during the second catalytic step of pre-mRNA splicing",
      "volume": "1",
      "author": "Umen",
      "year": "1995",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib65",
      "doi-asserted-by": "crossref",
      "first-page": "149",
      "DOI": "10.1016/S0092-8674(02)00726-2",
      "article-title": "Spliceosomal snRNAs: Mg(2+)-dependent chemistry at the catalytic core?",
      "volume": "109",
      "author": "Villa",
      "year": "2002",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib66",
      "doi-asserted-by": "crossref",
      "first-page": "1918",
      "DOI": "10.1126/science.1411506",
      "article-title": "Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing",
      "volume": "257",
      "author": "Wassarman",
      "year": "1992",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib67",
      "doi-asserted-by": "crossref",
      "first-page": "7219",
      "DOI": "10.1128/MCB.14.11.7219",
      "article-title": "Physical isolation of nascent RNA chains transcribed by RNA polymerase II: evidence for cotranscriptional splicing",
      "volume": "14",
      "author": "Wuarin",
      "year": "1994",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib68",
      "doi-asserted-by": "crossref",
      "first-page": "2542",
      "DOI": "10.1101/gad.6.12b.2542",
      "article-title": "Site-specific cross-linking of mammalian U5 snRNP to the 5\u2032 splice site before the first step of pre-mRNA splicing",
      "volume": "6",
      "author": "Wyatt",
      "year": "1992",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib69",
      "doi-asserted-by": "crossref",
      "first-page": "581",
      "DOI": "10.1101/gad.13.5.581",
      "article-title": "Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex",
      "volume": "13",
      "author": "Zhang",
      "year": "1999",
      "journal-title": "Genes Dev."
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib70",
      "doi-asserted-by": "crossref",
      "first-page": "319",
      "DOI": "10.1016/S1097-2765(01)00180-0",
      "article-title": "A biochemical function for the Sm complex",
      "volume": "7",
      "author": "Zhang",
      "year": "2001",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.molcel.2005.05.006_bib71",
      "doi-asserted-by": "crossref",
      "first-page": "30",
      "DOI": "10.1101/gad.851701",
      "article-title": "Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56",
      "volume": "15",
      "author": "Zhang",
      "year": "2001",
      "journal-title": "Genes Dev."
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  "container-title-short": "Molecular Cell",
  "published": {
    "date-parts": [
      [
        2005,
        7
      ]
    ]
  }
}