DOI: 10.1016/j.jsb.2021.107743. A streamlined workflow for automated cryo focused ion beam milling

A streamlined workflow for automated cryo focused ion beam milling

10.1016/j.jsb.2021.107743

Crossref journal-article

Elsevier BV

Journal of Structural Biology (78)

Bibliography

Tacke, S., Erdmann, P., Wang, Z., Klumpe, S., Grange, M., Plitzko, J., & Raunser, S. (2021). A streamlined workflow for automated cryo focused ion beam milling. Journal of Structural Biology, 213(3), 107743.

Authors 7

Sebastian Tacke (first)
Philipp Erdmann (additional)
Zhexin Wang (additional)
Sven Klumpe (additional)
Michael Grange (additional)
Jürgen Plitzko (additional)
Stefan Raunser (additional)

References 24 Referenced 88

10.1016/j.jsb.2005.01.003 / J. Struct. Biol. / Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy by Al-Alamoudi (2005)
10.1016/j.jsb.2004.03.010 / J. Struct. Biol. / Cryo-electron microscopy of vitreous sections of native biological cells and tissues by Al-Amoudi (2004)
10.1016/j.str.2012.10.016 / Structure / Secondary Structure Determination by Constrained Single-Particle Cryo-Electron Tomography by Bartesaghi (2012)
{'year': '2017', 'series-title': 'Vacuum and Ultravacuum: Physics and Technology', 'author': 'Bello', 'key': '10.1016/j.jsb.2021.107743_b0020'} / Vacuum and Ultravacuum: Physics and Technology by Bello (2017)
10.1016/j.molcel.2010.08.003 / Mol. Cell / The Three-Dimensional Organization of Polyribosomes in Intact Human Cells by Brandt (2010)
10.1016/j.jsb.2020.107488 / J. Struct. Biol. / Automated cryo-lamella preparation for high-throughput in-situ structural biology by Buckley (2020)
{'year': '1992', 'series-title': 'Low-Temperature Microscopy and Analysis', 'author': 'Echling', 'key': '10.1016/j.jsb.2021.107743_b0035'} / Low-Temperature Microscopy and Analysis by Echling (1992)
10.1016/j.jsb.2016.06.024 / J. Struct. Biol. / Cellular electron cryo tomography and in situ sub-volume averaging reveal the context of microtubule-based processes by Grange (2017)
10.1016/j.cell.2015.11.029 / Cell / Structural basis of vesicle formation at the inner nuclear membrane by Hagen (2015)
10.1016/j.jsb.2011.12.003 / J. Struct. Biol. / PyTom: a python-based toolbox for localization of macromolecules in cryo-electron tomograms and subtomogram analysis by Hrabe (2012)
10.1006/jsbi.1996.0013 / J. Struct. Biol. / Computer visualization of three-dimensional image data using IMOD by Kremer (1996)
10.1126/science.aad8857 / Science / Visualizing the molecular sociology at the HeLa cell nuclear periphery by Mahamid (2016)
10.1038/nmeth1014 / Nat. Methods / Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy by Marko (2007)
10.1016/j.jsb.2005.07.007 / J. Struct. Biol. / Automated electron microscope tomography using robust prediction of specimen movements by Mastronarde (2005)
10.1016/j.ultramic.2018.04.002 / Ultramicroscopy / Robust workflow and instrumentation for cryo-focused ion beam milling of samples for electron cryotomography by Medeiros (2018)
10.1016/j.jsb.2010.02.011 / J. Struct. Biol. / Micromachining tools and correlative approaches for cellular cryo-electron tomography by Rigort (2010)
10.1016/j.jsb.2015.08.008 / J. Struct. Biol. / CTFFIND4: Fast and accurate defocus estimation from electron micrographs by Rohou (2015)
10.1126/science.aaf9620 / Science / An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation by Schur (2016)
10.1016/j.bpj.2016.01.024 / Biophysical Journal / A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics by Tacke (2016)
{'key': '10.1016/j.jsb.2021.107743_b0105', 'first-page': '9', 'article-title': 'Berechnung von Gefriertrocknungszeiten für die elektronenmikroskopische Präparation', 'volume': '40', 'author': 'Umrath', 'year': '1983', 'journal-title': 'Mikroskopie'} / Mikroskopie / Berechnung von Gefriertrocknungszeiten für die elektronenmikroskopische Präparation by Umrath (1983)
10.1016/j.jsb.2013.05.008 / J. Struct. Biol. / Image formation modelling in cryo-electron microscopy by Vulovic (2013)
10.1016/j.jsb.2019.09.006 / J. Struct. Biol. / Mind the gap: Micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae by Wolff (2019)
{'key': '10.1016/j.jsb.2021.107743_b0120', 'article-title': 'Fully automated, sequential focused ion beam milling for cryo-electron tomography', 'volume': '9', 'author': 'Zach', 'year': '2020', 'journal-title': 'eLife'} / eLife / Fully automated, sequential focused ion beam milling for cryo-electron tomography by Zach (2020)
10.7554/eLife.42166 / eLife / New tools for automated high-resolution cryo-EM structure determination in RELION-3 by Zivanov (2018)

Dates

Type	When
Created	4 years, 3 months ago (May 8, 2021, 5:12 a.m.)
Deposited	1 year, 3 months ago (May 8, 2024, 11:59 p.m.)
Indexed	14 hours, 48 minutes ago (Aug. 20, 2025, 8:54 a.m.)
Issued	3 years, 11 months ago (Sept. 1, 2021)
Published	3 years, 11 months ago (Sept. 1, 2021)
Published Print	3 years, 11 months ago (Sept. 1, 2021)

Funders 2

European Molecular Biology Organization 10.13039/100004410
Region: Europe
pri (International organizations)
Labels2
1. The European Molecular Biology Organization
2. EMBO
Max-Planck-Gesellschaft 10.13039/501100004189
Region: Europe
gov (Research institutes and centers)
Labels4
1. Max Planck Society for the Advancement of Science
2. Max-Planck-Gesellschaft zur Förderung der Wissenschaften
3. Max Planck Society
4. MPG

BibTeX

@article{Tacke_2021, title={A streamlined workflow for automated cryo focused ion beam milling}, volume={213}, ISSN={1047-8477}, url={http://dx.doi.org/10.1016/j.jsb.2021.107743}, DOI={10.1016/j.jsb.2021.107743}, number={3}, journal={Journal of Structural Biology}, publisher={Elsevier BV}, author={Tacke, Sebastian and Erdmann, Philipp and Wang, Zhexin and Klumpe, Sven and Grange, Michael and Plitzko, Jürgen and Raunser, Stefan}, year={2021}, month=sep, pages={107743} }

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      "label": "Copyright"
    }
  ],
  "article-number": "107743"
}