DOI: 10.1016/j.jsb.2014.12.002. Seeing tobacco mosaic virus through direct electron detectors

Seeing tobacco mosaic virus through direct electron detectors

10.1016/j.jsb.2014.12.002

Crossref journal-article

Elsevier BV

Journal of Structural Biology (78)

Bibliography

Fromm, S. A., Bharat, T. A. M., Jakobi, A. J., Hagen, W. J. H., & Sachse, C. (2015). Seeing tobacco mosaic virus through direct electron detectors. Journal of Structural Biology, 189(2), 87â97.

Authors 5

Simon A. Fromm (first)
Tanmay A.M. Bharat (additional)
Arjen J. Jakobi (additional)
Wim J.H. Hagen (additional)
Carsten Sachse (additional)

References 51 Referenced 90

{'issue': '2010', 'key': '10.1016/j.jsb.2014.12.002_b0005', 'first-page': '213', 'article-title': 'PHENIX: a comprehensive python-based system for macromolecular structure solution', 'volume': 'D66', 'author': 'Adams', 'year': '2010', 'journal-title': 'Acta Crystallogr.'} / Acta Crystallogr. / PHENIX: a comprehensive python-based system for macromolecular structure solution by Adams (2010)
10.1038/308032a0 / Nature / Cryo-electron microscopy of viruses by Adrian (1984)
10.7554/eLife.01963 / eLife / Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector by Allegretti (2014)
10.1126/science.1249410 / Science / Structure of the yeast mitochondrial large ribosomal subunit by Amunts (2014)
10.7554/eLife.00461 / eLife / Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles by Bai (2013)
10.1073/pnas.1402809111 / Proc. Natl. Acad. Sci. U.S.A. / Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy by Bartesaghi (2014)
10.1085/jgp.25.1.147 / J. Gen. Physiol. / X-ray and crystallographic studies of plant virus preparations. III by Bernal (1941)
10.1016/j.jsb.2012.02.003 / J. Struct. Biol. / Beam-induced motion of vitrified specimen on holey carbon film by Brilot (2012)
10.1016/j.str.2012.08.026 / Structure / Movies of ice-embedded particles enhance resolution in electron cryo-microscopy by Campbell (2012)
10.1016/j.ultramic.2013.06.004 / Ultramicroscopy by Chen (2013)
10.1016/j.jsb.2010.06.011 / J. Struct. Biol. / 4.6Å Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300keV on a 4k×4k CCD camera by Clare (2009)
10.1016/0304-3991(94)90165-1 / Ultramicroscopy / High-resolution electron microscopy of biological specimens in cubic ice by Cyrklaff (1994)
10.1016/j.jsb.2013.11.003 / J. Struct. Biol. / SPRING – an image processing package for single-particle based helical reconstruction from electron cryomicrographs by Desfosses (2014)
10.1016/S0304-3991(00)00062-0 / Ultramicroscopy / A robust algorithm for the reconstruction of helical filaments using single-particle methods by Egelman (2000)
10.1107/S0907444904019158 / Acta Crystallogr. D Biol. Crystallogr. / Coot: model-building tools for molecular graphics by Emsley (2004)
10.1016/j.sbi.2007.08.014 / Curr. Opin. Struct. Biol. / Electronic detectors for electron microscopy by Faruqi (2007)
10.1126/science.1240585 / Science / Molecular architecture of a eukaryotic translational initiation complex by Fernandez (2013)
10.1073/pnas.1018104108 / Proc. Natl. Acad. Sci. U.S.A. / Hydrogen-bonding networks and RNA bases revealed by cryo electron microscopy suggest a triggering mechanism for calcium switches by Ge (2011)
10.7554/eLife.00573 / eLife / Direct detection pays off for electron cryo-microscopy by Grigorieff (2013)
10.1006/jmbi.1996.0328 / J. Mol. Biol. / Electron-crystallographic refinement of the structure of bacteriorhodopsin by Grigorieff (1996)
10.1098/rspb.1990.0057 / Proc. R. Soc. Lond. B Biol. Sci. / Cryo-protection of protein crystals against radiation damage in electron and X-ray diffraction by Henderson (1990)
10.1016/S0022-2836(05)80271-2 / J. Mol. Biol. / Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy by Henderson (1990)
ICRU (1984). Stopping Powers for Electrons and Positrons (Report 37) (Bethesda, Maryland, USA)
10.1107/S090904951100820X / J. Synchrotron Radiat. / Radiation damage by Karuppasamy (2011)
10.1038/nmeth.2727 / Nat. Methods / Quantifying the local resolution of cryo-EM density maps by Kucukelbir (2014)
10.1038/nmeth.2472 / Nat. Methods / Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM by Li (2013)
10.1016/j.jsb.2013.08.005 / J. Struct. Biol. by Li (2013)
10.1038/nature12822 / Nature / Structure of the TRPV1 ion channel determined by electron cryo-microscopy by Liao (2013)
10.1038/nature13567 / Nature / Three-dimensional structure of human γ-secretase by Lu (2014)
10.1016/j.jsb.2005.07.007 / J. Struct. Biol. / Automated electron microscope tomography using robust prediction of specimen movements by Mastronarde (2005)
10.1016/j.ultramic.2014.08.002 / Ultramicroscopy / Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy by McMullan (2014)
10.1016/S1047-8477(03)00069-8 / J. Struct. Biol. / Accurate determination of local defocus and specimen tilt in electron microscopy by Mindell (2003)
10.1073/pnas.0600973103 / Proc. Natl. Acad. Sci. U.S.A. / Experimental determination of the radiation dose limit for cryocooled protein crystals by Owen (2006)
10.1002/jcc.20084 / J. Comput. Chem. / UCSF Chimera – a visualization system for exploratory research and analysis by Pettersen (2004)
10.1016/j.sbi.2006.08.001 / Curr. Opin. Struct. Biol. / Radiation damage in macromolecular cryocrystallography by Ravelli (2006)
10.1016/j.jmb.2003.07.013 / J. Mol. Biol. / Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy by Rosenthal (2003)
10.1016/j.jsb.2013.10.016 / J. Struct. Biol. / Quantitative characterization of electron detectors for transmission electron microscopy by Ruskin (2013)
10.1016/j.jmb.2007.05.088 / J. Mol. Biol. / High-resolution electron microscopy of helical specimens: a fresh look at tobacco mosaic virus by Sachse (2007)
10.7554/eLife.03665 / eLife / Beam-induced motion correction for sub-megadalton cryo-EM particles by Scheres (2014)
10.1038/nmeth.2115 / Nat. Methods / Prevention of overfitting in cryo-EM structure determination by Scheres (2012)
10.1016/j.ultramic.2013.04.001 / Ultramicroscopy / Noise models and cryo-EM drift correction with a direct-electron camera by Shigematsu (2013)
10.1016/j.jsb.2006.05.009 / J. Struct. Biol. / EMAN2: an extensible image processing suite for electron microscopy by Tang (2007)
10.1016/j.jmb.2004.12.031 / J. Mol. Biol. / Refined structure of the nicotinic acetylcholine receptor at 4A resolution by Unwin (2005)
10.1016/j.str.2014.01.011 / Structure / Molecular mechanism of antibody-mediated activation of β-galactosidase by Vinothkumar (2014)
10.1016/j.cell.2014.05.024 / Cell / Structure of the Mammalian ribosome-sec61 complex to 3.4Å resolution by Voorhees (2014)
10.1038/ncomms5808 / Nat. Commun. / An atomic model of brome mosaic virus using direct electron detection and real-space optimization by Wang (2014)
10.1073/pnas.97.2.623 / Proc. Natl. Acad. Sci. U.S.A. / Specific chemical and structural damage to proteins produced by synchrotron radiation by Weik (2000)
10.1107/S0907444910045749 / Acta Crystallogr. D Biol. Crystallogr. / Overview of the CCP4 suite and current developments by Winn (2011)
10.1038/nature06893 / Nature / 3.88A structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy by Yu (2008)
10.1016/j.jsb.2011.05.004 / J. Struct. Biol. / Limiting factors in atomic resolution cryo electron microscopy: no simple tricks by Zhang (2011)
10.1073/pnas.0711623105 / Proc. Natl. Acad. Sci. U.S.A. / Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction by Zhang (2008)

Dates

Type	When
Created	10 years, 8 months ago (Dec. 19, 2014, 7:58 a.m.)
Deposited	6 years, 11 months ago (Sept. 26, 2018, 6:20 p.m.)
Indexed	15 hours, 5 minutes ago (Sept. 4, 2025, 9:18 a.m.)
Issued	10 years, 7 months ago (Feb. 1, 2015)
Published	10 years, 7 months ago (Feb. 1, 2015)
Published Print	10 years, 7 months ago (Feb. 1, 2015)

Funders 1

EMBL Interdisciplinary Postdoc
Awards1
1. PCOFUND-GA-2008-229597

BibTeX

@article{Fromm_2015, title={Seeing tobacco mosaic virus through direct electron detectors}, volume={189}, ISSN={1047-8477}, url={http://dx.doi.org/10.1016/j.jsb.2014.12.002}, DOI={10.1016/j.jsb.2014.12.002}, number={2}, journal={Journal of Structural Biology}, publisher={Elsevier BV}, author={Fromm, Simon A. and Bharat, Tanmay A.M. and Jakobi, Arjen J. and Hagen, Wim J.H. and Sachse, Carsten}, year={2015}, month=feb, pages={87–97} }

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      "volume": "3",
      "author": "Allegretti",
      "year": "2014",
      "journal-title": "eLife"
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      "first-page": "1485",
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      "author": "Amunts",
      "year": "2014",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0025",
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      "first-page": "e00461",
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      "article-title": "Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles",
      "volume": "2",
      "author": "Bai",
      "year": "2013",
      "journal-title": "eLife"
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    {
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      "first-page": "11709",
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      "volume": "111",
      "author": "Bartesaghi",
      "year": "2014",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0035",
      "doi-asserted-by": "crossref",
      "first-page": "147",
      "DOI": "10.1085/jgp.25.1.147",
      "article-title": "X-ray and crystallographic studies of plant virus preparations. III",
      "volume": "25",
      "author": "Bernal",
      "year": "1941",
      "journal-title": "J. Gen. Physiol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0040",
      "doi-asserted-by": "crossref",
      "first-page": "630",
      "DOI": "10.1016/j.jsb.2012.02.003",
      "article-title": "Beam-induced motion of vitrified specimen on holey carbon film",
      "volume": "177",
      "author": "Brilot",
      "year": "2012",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0045",
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      "first-page": "1823",
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      "volume": "20",
      "author": "Campbell",
      "year": "2012",
      "journal-title": "Structure"
    },
    {
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      "volume": "135",
      "author": "Chen",
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    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0055",
      "doi-asserted-by": "crossref",
      "first-page": "303",
      "DOI": "10.1016/j.jsb.2010.06.011",
      "article-title": "4.6\u00c5 Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300keV on a 4k\u00d74k CCD camera",
      "volume": "171",
      "author": "Clare",
      "year": "2009",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0060",
      "doi-asserted-by": "crossref",
      "first-page": "141",
      "DOI": "10.1016/0304-3991(94)90165-1",
      "article-title": "High-resolution electron microscopy of biological specimens in cubic ice",
      "volume": "55",
      "author": "Cyrklaff",
      "year": "1994",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0065",
      "doi-asserted-by": "crossref",
      "first-page": "15",
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      "volume": "185",
      "author": "Desfosses",
      "year": "2014",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0070",
      "doi-asserted-by": "crossref",
      "first-page": "225",
      "DOI": "10.1016/S0304-3991(00)00062-0",
      "article-title": "A robust algorithm for the reconstruction of helical filaments using single-particle methods",
      "volume": "85",
      "author": "Egelman",
      "year": "2000",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0075",
      "doi-asserted-by": "crossref",
      "first-page": "2126",
      "DOI": "10.1107/S0907444904019158",
      "article-title": "Coot: model-building tools for molecular graphics",
      "volume": "60",
      "author": "Emsley",
      "year": "2004",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0080",
      "doi-asserted-by": "crossref",
      "first-page": "549",
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      "author": "Faruqi",
      "year": "2007",
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      "author": "Fernandez",
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      "journal-title": "Science"
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      "volume": "108",
      "author": "Ge",
      "year": "2011",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
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    {
      "key": "10.1016/j.jsb.2014.12.002_b0095",
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      "author": "Grigorieff",
      "year": "2013",
      "journal-title": "eLife"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0100",
      "doi-asserted-by": "crossref",
      "first-page": "393",
      "DOI": "10.1006/jmbi.1996.0328",
      "article-title": "Electron-crystallographic refinement of the structure of bacteriorhodopsin",
      "volume": "259",
      "author": "Grigorieff",
      "year": "1996",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0105",
      "doi-asserted-by": "crossref",
      "first-page": "6",
      "DOI": "10.1098/rspb.1990.0057",
      "article-title": "Cryo-protection of protein crystals against radiation damage in electron and X-ray diffraction",
      "volume": "241",
      "author": "Henderson",
      "year": "1990",
      "journal-title": "Proc. R. Soc. Lond. B Biol. Sci."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0110",
      "doi-asserted-by": "crossref",
      "first-page": "899",
      "DOI": "10.1016/S0022-2836(05)80271-2",
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      "volume": "213",
      "author": "Henderson",
      "year": "1990",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0115",
      "unstructured": "ICRU (1984). Stopping Powers for Electrons and Positrons (Report 37) (Bethesda, Maryland, USA)"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0120",
      "doi-asserted-by": "crossref",
      "first-page": "398",
      "DOI": "10.1107/S090904951100820X",
      "article-title": "Radiation damage",
      "volume": "18",
      "author": "Karuppasamy",
      "year": "2011",
      "journal-title": "J. Synchrotron Radiat."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0125",
      "doi-asserted-by": "crossref",
      "first-page": "63",
      "DOI": "10.1038/nmeth.2727",
      "article-title": "Quantifying the local resolution of cryo-EM density maps",
      "volume": "11",
      "author": "Kucukelbir",
      "year": "2014",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0130",
      "doi-asserted-by": "crossref",
      "first-page": "584",
      "DOI": "10.1038/nmeth.2472",
      "article-title": "Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM",
      "volume": "10",
      "author": "Li",
      "year": "2013",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0135",
      "doi-asserted-by": "crossref",
      "first-page": "251",
      "DOI": "10.1016/j.jsb.2013.08.005",
      "volume": "184",
      "author": "Li",
      "year": "2013",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0140",
      "doi-asserted-by": "crossref",
      "first-page": "107",
      "DOI": "10.1038/nature12822",
      "article-title": "Structure of the TRPV1 ion channel determined by electron cryo-microscopy",
      "volume": "504",
      "author": "Liao",
      "year": "2013",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0145",
      "doi-asserted-by": "crossref",
      "first-page": "166",
      "DOI": "10.1038/nature13567",
      "article-title": "Three-dimensional structure of human \u03b3-secretase",
      "volume": "512",
      "author": "Lu",
      "year": "2014",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0150",
      "doi-asserted-by": "crossref",
      "first-page": "36",
      "DOI": "10.1016/j.jsb.2005.07.007",
      "article-title": "Automated electron microscope tomography using robust prediction of specimen movements",
      "volume": "152",
      "author": "Mastronarde",
      "year": "2005",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0155",
      "doi-asserted-by": "crossref",
      "first-page": "156",
      "DOI": "10.1016/j.ultramic.2014.08.002",
      "article-title": "Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy",
      "volume": "147",
      "author": "McMullan",
      "year": "2014",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0160",
      "doi-asserted-by": "crossref",
      "first-page": "334",
      "DOI": "10.1016/S1047-8477(03)00069-8",
      "article-title": "Accurate determination of local defocus and specimen tilt in electron microscopy",
      "volume": "142",
      "author": "Mindell",
      "year": "2003",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0165",
      "doi-asserted-by": "crossref",
      "first-page": "4912",
      "DOI": "10.1073/pnas.0600973103",
      "article-title": "Experimental determination of the radiation dose limit for cryocooled protein crystals",
      "volume": "103",
      "author": "Owen",
      "year": "2006",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0170",
      "doi-asserted-by": "crossref",
      "first-page": "1605",
      "DOI": "10.1002/jcc.20084",
      "article-title": "UCSF Chimera \u2013 a visualization system for exploratory research and analysis",
      "volume": "25",
      "author": "Pettersen",
      "year": "2004",
      "journal-title": "J. Comput. Chem."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0175",
      "doi-asserted-by": "crossref",
      "first-page": "624",
      "DOI": "10.1016/j.sbi.2006.08.001",
      "article-title": "Radiation damage in macromolecular cryocrystallography",
      "volume": "16",
      "author": "Ravelli",
      "year": "2006",
      "journal-title": "Curr. Opin. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0180",
      "doi-asserted-by": "crossref",
      "first-page": "721",
      "DOI": "10.1016/j.jmb.2003.07.013",
      "article-title": "Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy",
      "volume": "333",
      "author": "Rosenthal",
      "year": "2003",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0185",
      "doi-asserted-by": "crossref",
      "first-page": "385",
      "DOI": "10.1016/j.jsb.2013.10.016",
      "article-title": "Quantitative characterization of electron detectors for transmission electron microscopy",
      "volume": "184",
      "author": "Ruskin",
      "year": "2013",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0190",
      "doi-asserted-by": "crossref",
      "first-page": "812",
      "DOI": "10.1016/j.jmb.2007.05.088",
      "article-title": "High-resolution electron microscopy of helical specimens: a fresh look at tobacco mosaic virus",
      "volume": "371",
      "author": "Sachse",
      "year": "2007",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0195",
      "doi-asserted-by": "crossref",
      "first-page": "e03665",
      "DOI": "10.7554/eLife.03665",
      "article-title": "Beam-induced motion correction for sub-megadalton cryo-EM particles",
      "volume": "3",
      "author": "Scheres",
      "year": "2014",
      "journal-title": "eLife"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0200",
      "doi-asserted-by": "crossref",
      "first-page": "853",
      "DOI": "10.1038/nmeth.2115",
      "article-title": "Prevention of overfitting in cryo-EM structure determination",
      "volume": "9",
      "author": "Scheres",
      "year": "2012",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0205",
      "doi-asserted-by": "crossref",
      "first-page": "61",
      "DOI": "10.1016/j.ultramic.2013.04.001",
      "article-title": "Noise models and cryo-EM drift correction with a direct-electron camera",
      "volume": "131",
      "author": "Shigematsu",
      "year": "2013",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0210",
      "doi-asserted-by": "crossref",
      "first-page": "38",
      "DOI": "10.1016/j.jsb.2006.05.009",
      "article-title": "EMAN2: an extensible image processing suite for electron microscopy",
      "volume": "157",
      "author": "Tang",
      "year": "2007",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0215",
      "doi-asserted-by": "crossref",
      "first-page": "967",
      "DOI": "10.1016/j.jmb.2004.12.031",
      "article-title": "Refined structure of the nicotinic acetylcholine receptor at 4A resolution",
      "volume": "346",
      "author": "Unwin",
      "year": "2005",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0220",
      "doi-asserted-by": "crossref",
      "first-page": "621",
      "DOI": "10.1016/j.str.2014.01.011",
      "article-title": "Molecular mechanism of antibody-mediated activation of \u03b2-galactosidase",
      "volume": "22",
      "author": "Vinothkumar",
      "year": "2014",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0225",
      "doi-asserted-by": "crossref",
      "first-page": "1632",
      "DOI": "10.1016/j.cell.2014.05.024",
      "article-title": "Structure of the Mammalian ribosome-sec61 complex to 3.4\u00c5 resolution",
      "volume": "157",
      "author": "Voorhees",
      "year": "2014",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0230",
      "doi-asserted-by": "crossref",
      "first-page": "4808",
      "DOI": "10.1038/ncomms5808",
      "article-title": "An atomic model of brome mosaic virus using direct electron detection and real-space optimization",
      "volume": "5",
      "author": "Wang",
      "year": "2014",
      "journal-title": "Nat. Commun."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0235",
      "doi-asserted-by": "crossref",
      "first-page": "623",
      "DOI": "10.1073/pnas.97.2.623",
      "article-title": "Specific chemical and structural damage to proteins produced by synchrotron radiation",
      "volume": "97",
      "author": "Weik",
      "year": "2000",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0240",
      "doi-asserted-by": "crossref",
      "first-page": "235",
      "DOI": "10.1107/S0907444910045749",
      "article-title": "Overview of the CCP4 suite and current developments",
      "volume": "67",
      "author": "Winn",
      "year": "2011",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0245",
      "doi-asserted-by": "crossref",
      "first-page": "415",
      "DOI": "10.1038/nature06893",
      "article-title": "3.88A structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy",
      "volume": "453",
      "author": "Yu",
      "year": "2008",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0250",
      "doi-asserted-by": "crossref",
      "first-page": "253",
      "DOI": "10.1016/j.jsb.2011.05.004",
      "article-title": "Limiting factors in atomic resolution cryo electron microscopy: no simple tricks",
      "volume": "175",
      "author": "Zhang",
      "year": "2011",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2014.12.002_b0255",
      "doi-asserted-by": "crossref",
      "first-page": "1867",
      "DOI": "10.1073/pnas.0711623105",
      "article-title": "Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction",
      "volume": "105",
      "author": "Zhang",
      "year": "2008",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    }
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