DOI: 10.1016/j.jsb.2013.10.019. Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography

Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography

10.1016/j.jsb.2013.10.019

Crossref journal-article

Elsevier BV

Journal of Structural Biology (78)

Bibliography

Hsieh, C., Schmelzer, T., Kishchenko, G., Wagenknecht, T., & Marko, M. (2014). Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography. Journal of Structural Biology, 185(1), 32â41.

Authors 5

Chyongere Hsieh (first)
Thomas Schmelzer (additional)
Gregory Kishchenko (additional)
Terence Wagenknecht (additional)
Michael Marko (additional)

References 33 Referenced 78

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10.1016/j.jsb.2013.05.016 / J. Struct. Biol. / In-situ integrity control of frozen-hydrated, vitreous lamellas prepared by the cryo-focused ion beam-scanning electron microscope by de Winter (2013)
10.1111/j.1365-2818.2009.03152.x / J. Microsc. / An appraisal of ultramicrotomy, FIBSEM and cryogenic FIBSEM techniques for the sectioning of biological cells on titanium substrates for TEM investigation by Edwards (2009)
10.1016/j.jsb.2007.07.006 / J. Struct. Biol. / Classification of cryoelectron subtomograms using constrained correlation by Förster (2008)
{'key': '10.1016/j.jsb.2013.10.019_b0035', 'series-title': 'Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in their Native State', 'author': 'Frank', 'year': '2006'} / Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in their Native State by Frank (2006)
10.1111/j.1365-2818.2007.01775.x / J. Microsc. / Technique for improved focused ion beam milling of cryo-prepared life science specimens by Hayles (2007)
10.1016/j.jsb.2010.07.004 / J. Struct. Biol. / The making of frozen-hydrated, vitreous lamellas from cells for cryoelectron microscopy by Hayles (2010)
10.1016/j.jsb.2011.05.011 / J. Struct. Biol. / Clustering and variance maps for cryo-electron tomography using wedge-masked differences by Heumann (2011)
10.1016/j.jsb.2005.10.004 / J. Struct. Biol. / Towards high resolution three-dimensional imaging of native mammalian tissue: electron tomography of frozen-hydrated rat liver sections by Hsieh (2006)
10.1006/jsbi.1996.0013 / J. Struct. Biol. / Computer visualization of three-dimensional image data using IMOD by Kremer (1996)
10.1016/j.jsb.2012.10.010 / J. Struct. Biol. / A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography by Kuybeda (2013)
{'issue': 'Suppl 2', 'key': '10.1016/j.jsb.2013.10.019_b0070', 'article-title': 'Feasibility of focused ion beam milling for preparation of TEM specimens of biological material embedded in vitreous ice', 'volume': '11', 'author': 'Marko', 'year': '2005', 'journal-title': 'Microsc. Microanal.'} / Microsc. Microanal. / Feasibility of focused ion beam milling for preparation of TEM specimens of biological material embedded in vitreous ice by Marko (2005)
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Dates

Type	When
Created	11 years, 9 months ago (Nov. 6, 2013, 2:06 p.m.)
Deposited	6 years, 10 months ago (Oct. 10, 2018, 9:20 p.m.)
Indexed	28 minutes ago (Aug. 20, 2025, 8:53 p.m.)
Issued	11 years, 7 months ago (Jan. 1, 2014)
Published	11 years, 7 months ago (Jan. 1, 2014)
Published Print	11 years, 7 months ago (Jan. 1, 2014)

Funders 1

NIH
Awards2
1. R01AR040615
2. R01GM097010

BibTeX

@article{Hsieh_2014, title={Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography}, volume={185}, ISSN={1047-8477}, url={http://dx.doi.org/10.1016/j.jsb.2013.10.019}, DOI={10.1016/j.jsb.2013.10.019}, number={1}, journal={Journal of Structural Biology}, publisher={Elsevier BV}, author={Hsieh, Chyongere and Schmelzer, Thomas and Kishchenko, Gregory and Wagenknecht, Terence and Marko, Michael}, year={2014}, month=jan, pages={32–41} }

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