DOI: 10.1016/j.jsb.2005.07.005. Epoxy resin as fixative during freeze-substitution

Epoxy resin as fixative during freeze-substitution

10.1016/j.jsb.2005.07.005

Crossref journal-article

Elsevier BV

Journal of Structural Biology (78)

Bibliography

Matsko, N., & Mueller, M. (2005). Epoxy resin as fixative during freeze-substitution. Journal of Structural Biology, 152(2), 92â103.

Authors 2

Nadezda Matsko (first)
Martin Mueller (additional)

References 22 Referenced 49

10.1038/sj.emboj.7600366 / EMBO J. / Cryo-electron microscopy of vitreous sections by Al-Amoudi (2004)
10.1046/j.1365-2818.2003.01244.x / J. Microsc. / An oscillating cryoknife reduces cutting-induced deformation of vitreous ultrathin sections by Al-Amoudi (2003)
10.1002/(SICI)1097-0029(20000315)48:6<385::AID-JEMT8>3.0.CO;2-R / Microsc. Res. Tech. / Ultrastructure of the echinoderm cuticle after fast-freezing/freeze-substitution and conventional chemical fixations by Ameye (2000)
10.1016/S0959-440X(02)00378-0 / Curr. Opin. Struct. Biol. / Electron tomography: towards visualizing the molecular organization of the cytoplasm by Baumeister (2002)
Causton, B.E., 1985. Does the embedding chemistry interact with tissue? In: Mueller M., Becker, R.P., Boyde, A., Wolosewick, J.J. (Eds.), The Science of Biological Specimen Preparation for Microscopy and Microanalysis. Scanning Electron Microscopy, AMF O’Hare 1985, pp. 209–214.
10.1046/j.1365-2818.2002.01033.x / J. Microsc. / Freeze-dried and resin-embedded biological material is well suited for ultrastructure research by Edelmann (2002)
10.1046/j.1365-2818.2003.01228.x / J. Microsc. / Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples by Giddings (2003)
{'key': '10.1016/j.jsb.2005.07.005_bib8', 'series-title': 'Principles and Techniques of Electron Microscopy: Biological Applications', 'author': 'Hayat', 'year': '2000'} / Principles and Techniques of Electron Microscopy: Biological Applications by Hayat (2000)
10.1111/j.1365-2818.1994.tb04785.x / J.Microsc. / High-pressure freezing of cell suspensions in cellulose capillary tubes by Hohenberg (1994)
Humbel, B., Mueller M., 1986. Freeze-substitution and low temperature embedding, In: Mueller, M., Becker, R.P., Boyde, A., Wolosewick, J.J. (Eds.), The Science of Biological Specimen Preparation for Microscopy and Microanalysis. Scanning Electron Microscopy, AMF O’Hare 1985, pp. 175–183.
10.1083/jcb.98.1.267 / J. Cell Biol. / Cartilage ultrastructure after high-pressure freezing, freeze-substitution, and low temperature embedding. I. Chondrocyte ultrastructure-implications for the theories of mineralization and vascular invasion by Hunziker (1984)
10.1038/378196a0 / Nature / Defective recycling of synaptic vesicles in synaptotagmin mutants of Caenorhabditis elegans by Jorgensen (1995)
10.1177/35.9.3302020 / J. Histochem. Cytochem. / The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions by Kellenberger (1987)
10.1146/annurev.matsci.27.1.175 / Annu. Rev. Mater. Sci. / Characterization of polymer surfaces with atomic force microscopy by Magonov (1997)
10.1016/j.jsb.2004.01.010 / J. Struct. Biol. / AFM of biological material embedded in epoxy resin by Matsko (2004)
10.1111/j.1365-2818.1992.tb01506.x / J. Microsc. / Diamonds are a cryosectioner’s best friend by Michel (1992)
10.1046/j.1365-2818.1998.00387.x / J. Microsc. / High-pressure freezing for immunocytochemistry by Monaghan (1998)
10.1046/j.1365-2818.2003.01245.x / J. Microsc. / High-pressure freezing in the study of animal pathogens by Monaghan (2003)
10.1083/jcb.17.1.208 / J. Cell Biol. / The use of lead citrate at high pH as an electron-opaque stain in electron microscopy by Reynolds (1963)
10.1111/j.1365-2818.1995.tb03648.x / J Microsc. / Vitrification of articular cartilage by high-pressure freezing by Studer (1995)
10.1002/ar.1091490307 / Anat. Rec. / Electron microscopy after rapid freezing on a metal surface and substitution fixation by Van Harreveld (1964)
10.1002/jemt.1098 / Microsc. Res. Tech. / Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze-substitution by Wild (2001)

Dates

Type	When
Created	20 years ago (Aug. 20, 2005, 3:18 a.m.)
Deposited	6 years, 6 months ago (Jan. 22, 2019, 12:50 p.m.)
Indexed	1 month, 1 week ago (July 9, 2025, 7:02 p.m.)
Issued	19 years, 9 months ago (Nov. 1, 2005)
Published	19 years, 9 months ago (Nov. 1, 2005)
Published Print	19 years, 9 months ago (Nov. 1, 2005)

Funders 0

None

BibTeX

@article{Matsko_2005, title={Epoxy resin as fixative during freeze-substitution}, volume={152}, ISSN={1047-8477}, url={http://dx.doi.org/10.1016/j.jsb.2005.07.005}, DOI={10.1016/j.jsb.2005.07.005}, number={2}, journal={Journal of Structural Biology}, publisher={Elsevier BV}, author={Matsko, Nadezda and Mueller, Martin}, year={2005}, month=nov, pages={92–103} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        9
      ]
    ],
    "date-time": "2025-07-09T23:02:04Z",
    "timestamp": 1752102124996
  },
  "reference-count": 22,
  "publisher": "Elsevier BV",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            11,
            1
          ]
        ],
        "date-time": "2005-11-01T00:00:00Z",
        "timestamp": 1130803200000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2005,
        11
      ]
    ]
  },
  "DOI": "10.1016/j.jsb.2005.07.005",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        8,
        20
      ]
    ],
    "date-time": "2005-08-20T07:18:50Z",
    "timestamp": 1124522330000
  },
  "page": "92-103",
  "source": "Crossref",
  "is-referenced-by-count": 49,
  "title": "Epoxy resin as fixative during freeze-substitution",
  "prefix": "10.1016",
  "volume": "152",
  "author": [
    {
      "given": "Nadezda",
      "family": "Matsko",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Martin",
      "family": "Mueller",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "issue": "18",
      "key": "10.1016/j.jsb.2005.07.005_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "3583",
      "DOI": "10.1038/sj.emboj.7600366",
      "article-title": "Cryo-electron microscopy of vitreous sections",
      "volume": "23",
      "author": "Al-Amoudi",
      "year": "2004",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "26",
      "DOI": "10.1046/j.1365-2818.2003.01244.x",
      "article-title": "An oscillating cryoknife reduces cutting-induced deformation of vitreous ultrathin sections",
      "volume": "212",
      "author": "Al-Amoudi",
      "year": "2003",
      "journal-title": "J. Microsc."
    },
    {
      "issue": "6",
      "key": "10.1016/j.jsb.2005.07.005_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "385",
      "DOI": "10.1002/(SICI)1097-0029(20000315)48:6<385::AID-JEMT8>3.0.CO;2-R",
      "article-title": "Ultrastructure of the echinoderm cuticle after fast-freezing/freeze-substitution and conventional chemical fixations",
      "volume": "48",
      "author": "Ameye",
      "year": "2000",
      "journal-title": "Microsc. Res. Tech."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "679",
      "DOI": "10.1016/S0959-440X(02)00378-0",
      "article-title": "Electron tomography: towards visualizing the molecular organization of the cytoplasm",
      "volume": "12",
      "author": "Baumeister",
      "year": "2002",
      "journal-title": "Curr. Opin. Struct. Biol."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib5",
      "unstructured": "Causton, B.E., 1985. Does the embedding chemistry interact with tissue? In: Mueller M., Becker, R.P., Boyde, A., Wolosewick, J.J. (Eds.), The Science of Biological Specimen Preparation for Microscopy and Microanalysis. Scanning Electron Microscopy, AMF O\u2019Hare 1985, pp. 209\u2013214."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "5",
      "DOI": "10.1046/j.1365-2818.2002.01033.x",
      "article-title": "Freeze-dried and resin-embedded biological material is well suited for ultrastructure research",
      "volume": "207",
      "author": "Edelmann",
      "year": "2002",
      "journal-title": "J. Microsc."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "53",
      "DOI": "10.1046/j.1365-2818.2003.01228.x",
      "article-title": "Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples",
      "volume": "212",
      "author": "Giddings",
      "year": "2003",
      "journal-title": "J. Microsc."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib8",
      "series-title": "Principles and Techniques of Electron Microscopy: Biological Applications",
      "author": "Hayat",
      "year": "2000"
    },
    {
      "issue": "1",
      "key": "10.1016/j.jsb.2005.07.005_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "34",
      "DOI": "10.1111/j.1365-2818.1994.tb04785.x",
      "article-title": "High-pressure freezing of cell suspensions in cellulose capillary tubes",
      "volume": "175",
      "author": "Hohenberg",
      "year": "1994",
      "journal-title": "J.Microsc."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib10",
      "unstructured": "Humbel, B., Mueller M., 1986. Freeze-substitution and low temperature embedding, In: Mueller, M., Becker, R.P., Boyde, A., Wolosewick, J.J. (Eds.), The Science of Biological Specimen Preparation for Microscopy and Microanalysis. Scanning Electron Microscopy, AMF O\u2019Hare 1985, pp. 175\u2013183."
    },
    {
      "issue": "1",
      "key": "10.1016/j.jsb.2005.07.005_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "267",
      "DOI": "10.1083/jcb.98.1.267",
      "article-title": "Cartilage ultrastructure after high-pressure freezing, freeze-substitution, and low temperature embedding. I. Chondrocyte ultrastructure-implications for the theories of mineralization and vascular invasion",
      "volume": "98",
      "author": "Hunziker",
      "year": "1984",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "196",
      "DOI": "10.1038/378196a0",
      "article-title": "Defective recycling of synaptic vesicles in synaptotagmin mutants of Caenorhabditis elegans",
      "volume": "378",
      "author": "Jorgensen",
      "year": "1995",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "959",
      "DOI": "10.1177/35.9.3302020",
      "article-title": "The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions",
      "volume": "35",
      "author": "Kellenberger",
      "year": "1987",
      "journal-title": "J. Histochem. Cytochem."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "175",
      "DOI": "10.1146/annurev.matsci.27.1.175",
      "article-title": "Characterization of polymer surfaces with atomic force microscopy",
      "volume": "27",
      "author": "Magonov",
      "year": "1997",
      "journal-title": "Annu. Rev. Mater. Sci."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "334",
      "DOI": "10.1016/j.jsb.2004.01.010",
      "article-title": "AFM of biological material embedded in epoxy resin",
      "volume": "146",
      "author": "Matsko",
      "year": "2004",
      "journal-title": "J. Struct. Biol."
    },
    {
      "issue": "1",
      "key": "10.1016/j.jsb.2005.07.005_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "43",
      "DOI": "10.1111/j.1365-2818.1992.tb01506.x",
      "article-title": "Diamonds are a cryosectioner\u2019s best friend",
      "volume": "166",
      "author": "Michel",
      "year": "1992",
      "journal-title": "J. Microsc."
    },
    {
      "issue": "3",
      "key": "10.1016/j.jsb.2005.07.005_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "248",
      "DOI": "10.1046/j.1365-2818.1998.00387.x",
      "article-title": "High-pressure freezing for immunocytochemistry",
      "volume": "192",
      "author": "Monaghan",
      "year": "1998",
      "journal-title": "J. Microsc."
    },
    {
      "issue": "1",
      "key": "10.1016/j.jsb.2005.07.005_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "62",
      "DOI": "10.1046/j.1365-2818.2003.01245.x",
      "article-title": "High-pressure freezing in the study of animal pathogens",
      "volume": "212",
      "author": "Monaghan",
      "year": "2003",
      "journal-title": "J. Microsc."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib19",
      "doi-asserted-by": "crossref",
      "first-page": "208",
      "DOI": "10.1083/jcb.17.1.208",
      "article-title": "The use of lead citrate at high pH as an electron-opaque stain in electron microscopy",
      "volume": "17",
      "author": "Reynolds",
      "year": "1963",
      "journal-title": "J. Cell Biol."
    },
    {
      "issue": "3",
      "key": "10.1016/j.jsb.2005.07.005_bib20",
      "doi-asserted-by": "crossref",
      "first-page": "321",
      "DOI": "10.1111/j.1365-2818.1995.tb03648.x",
      "article-title": "Vitrification of articular cartilage by high-pressure freezing",
      "volume": "179",
      "author": "Studer",
      "year": "1995",
      "journal-title": "J Microsc."
    },
    {
      "key": "10.1016/j.jsb.2005.07.005_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "381",
      "DOI": "10.1002/ar.1091490307",
      "article-title": "Electron microscopy after rapid freezing on a metal surface and substitution fixation",
      "volume": "149",
      "author": "Van Harreveld",
      "year": "1964",
      "journal-title": "Anat. Rec."
    },
    {
      "issue": "4",
      "key": "10.1016/j.jsb.2005.07.005_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "313",
      "DOI": "10.1002/jemt.1098",
      "article-title": "Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze-substitution",
      "volume": "53",
      "author": "Wild",
      "year": "2001",
      "journal-title": "Microsc. Res. Tech."
    }
  ],
  "container-title": "Journal of Structural Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S1047847705001504?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S1047847705001504?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2019,
        1,
        22
      ]
    ],
    "date-time": "2019-01-22T17:50:36Z",
    "timestamp": 1548179436000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S1047847705001504"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2005,
        11
      ]
    ]
  },
  "references-count": 22,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          2005,
          11
        ]
      ]
    }
  },
  "alternative-id": [
    "S1047847705001504"
  ],
  "URL": "http://dx.doi.org/10.1016/j.jsb.2005.07.005",
  "relation": {},
  "ISSN": [
    "1047-8477"
  ],
  "subject": [],
  "container-title-short": "Journal of Structural Biology",
  "published": {
    "date-parts": [
      [
        2005,
        11
      ]
    ]
  }
}