DOI: 10.1016/j.jmb.2004.12.044. Crystal Structure of a Substrate Complex of Mycobacterium tuberculosis β-Ketoacyl-acyl Carrier Protein Synthase III (FabH) with Lauroyl-coenzyme A

Crystal Structure of a Substrate Complex of Mycobacterium tuberculosis β-Ketoacyl-acyl Carrier Protein Synthase III (FabH) with Lauroyl-coenzyme A

10.1016/j.jmb.2004.12.044

Crossref journal-article

Elsevier BV

Journal of Molecular Biology (78)

Bibliography

Musayev, F., Sachdeva, S., Neel Scarsdale, J., Reynolds, K. A., & Wright, H. T. (2005). Crystal Structure of a Substrate Complex of Mycobacterium tuberculosis Î²-Ketoacyl-acyl Carrier Protein Synthase III (FabH) with Lauroyl-coenzyme A. Journal of Molecular Biology, 346(5), 1313â1321.

Authors 5

Faik Musayev (first)
Sarbjot Sachdeva (additional)
J. Neel Scarsdale (additional)
K.A. Reynolds (additional)
H.T. Wright (additional)

References 31 Referenced 52

10.1073/pnas.94.11.5588 / Proc. Natl Acad. Sci. USA / Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities by Chirala (1997)
{'key': '10.1016/j.jmb.2004.12.044_bib2', 'series-title': 'E.coli and Salmonella, Cellular and Molecular Biology', 'first-page': '612', 'article-title': 'Biosynthesis of membrane lipids', 'author': 'Cronan', 'year': '1996'} / E.coli and Salmonella, Cellular and Molecular Biology / Biosynthesis of membrane lipids by Cronan (1996)
10.1016/0076-6879(75)35141-0 / Methods Enzymol. / Fatty acid synthases from Mycobacterium phlei by Bloch (1975)
10.1016/S0163-7827(98)00008-3 / Prog. Lipid Res. / Mycolic acids: structure, biosynthesis and physiological functions by Barry (1998)
10.1074/jbc.M010762200 / J. Biol. Chem. / Crystal structure of the Mycobacterium tuberculosis β-ketoacyl-acyl carrier protein synthase III by Scarsdale (2001)
10.1016/S0969-2126(00)00094-0 / Structure / The 1.8Å crystal structure and active-site architecture of β-ketoacyl-acyl carrier protein synthase III (FabH) from Escherichia coli by Davies (2000)
10.1006/jmbi.2000.4457 / J. Mol. Biol. / Refined structures of β-ketoacyl-acyl carrier protein synthase III by Qiu (2001)
10.1016/S0969-2126(94)00081-6 / Structure / The 2.8Å crystal structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: a five layered αβαβα structure constructed from two core domains of identical topology by Mathieu (1994)
10.1016/S0969-2126(01)00583-4 / Structure / Structures of β-ketoacyl-acyl carrier protein synthase I complexes with fatty acids elucidate its catalytic machinery by Olsen (2001)
10.1093/emboj/17.5.1183 / EMBO J. / Crystal structure of β-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes by Huang (1998)
10.1006/jmbi.2000.4272 / J. Mol. Biol. / The crystal structure of β-ketoacyl-acyl carrier protein synthase II from Synechocystis sp. at 1.54Å resolution and its relationship to other condensing enzymes by Moche (2001)
10.1038/11553 / Nature Struct. Biol. / Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis by Ferrer (1999)
10.1016/S1074-5521(00)00041-7 / Chem. Biol. / Structural control of polyketide formation in plant-specific polyketide synthases by Jez (2000)
10.1016/S0969-2126(02)00889-4 / Structure / Crystal structure of the priming β-ketosynthase from the R1128 polyketide biosynthetic pathway by Pan (2002)
10.1074/jbc.M003241200 / J. Biol. Chem. / Identification and substrate specificity of β-ketoacyl (acyl carrier protein) synthase III (mtFabH) from M.tuberculosis by Choi (2000)
10.1128/JB.185.14.4136-4143.2003 / J. Bacteriol. / The 1.3-Angstrom-resolution crystal structure of β-ketoacyl-acyl carrier protein synthase II from Streptococcus pneumoniae by Price (2003)
10.1007/BF00135313 / J. Comput. Aided Mol. Des. / HINT—a new method of empirical hydrophobic field calculation for CoMFA by Kellogg (1991)
10.1107/S0907444994003112 / Acta Crystallog. sect. D / The CCP4 suite: programs for protein crystallography (1994)
{'key': '10.1016/j.jmb.2004.12.044_bib19', 'series-title': 'Proceedings of CCP4 Study Weekend on Recent Advances in Phasing', 'article-title': 'Scaling of MAD Data', 'author': 'Evans', 'year': '1997'} / Proceedings of CCP4 Study Weekend on Recent Advances in Phasing / Scaling of MAD Data by Evans (1997)
{'key': '10.1016/j.jmb.2004.12.044_bib20', 'series-title': 'BioteX; A Suite of Programs for the Collection, Reduction, and Interpretation of Imaging Plate Data', 'author': 'Chen', 'year': '1995'} / BioteX; A Suite of Programs for the Collection, Reduction, and Interpretation of Imaging Plate Data by Chen (1995)
10.1107/S0907444998003254 / Acta Crystallog. sect. D / Crystallography & NMR system: a new software suite for macromolecular structure determination by Brunger (1998)
10.1107/S0907444996012255 / Acta Crystallog. sect. D / Refinement of macromolecular structures by the maximum-likelihood method by Murshudov (1997)
10.1107/S0021889887012755 / J. Appl. Crystallog. / Calculation of an omit map by Bhat (1988)
10.1107/S0108767390010224 / Acta Crystallog. sect. A / Improved methods for building protein models in electron density maps and the location of errors in these models by Jones (1991)
10.1016/0263-7855(87)80024-3 / J. Mol. Graph. / TOM: a Frodo subpackage for protein-ligand fitting with interactive energy minimization by Cambillau (1987)
10.1006/jsbi.1999.4094 / J. Struct. Biol. / XtalView Xfit: a versatile program for manipulating atomic coordinates and electron density by McRee (1999)
10.1107/S0021889892009944 / J. Appl. Crystallog. / Procheck: a program to check the stereochemical quality of protein structures by Laskowski (1993)
10.1038/381272a0 / Nature / Errors in protein structures by Hooft (1996)
10.1107/S0907444996001783 / Acta Crystallog. sect. D / Efficient rebuilding of protein structures by Kleywegt (1996)
10.1006/jmbi.1998.2400 / J. Mol. Biol. / Visualizing and quantifiying molecular goodness of fit: small probe contact dots with explicit hydrogen atoms by Word (1999)
10.1107/S0907444998006684 / Acta Crystallog. sect. D / SFCHECK: a unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model by Vaguine (1999)

Dates

Type	When
Created	20 years, 7 months ago (Jan. 6, 2005, 7:15 a.m.)
Deposited	4 years, 2 months ago (July 3, 2021, 1:51 p.m.)
Indexed	4 weeks ago (Aug. 7, 2025, 4:24 p.m.)
Issued	20 years, 6 months ago (March 1, 2005)
Published	20 years, 6 months ago (March 1, 2005)
Published Print	20 years, 6 months ago (March 1, 2005)

Funders 0

None

BibTeX

@article{Musayev_2005, title={Crystal Structure of a Substrate Complex of Mycobacterium tuberculosis β-Ketoacyl-acyl Carrier Protein Synthase III (FabH) with Lauroyl-coenzyme A}, volume={346}, ISSN={0022-2836}, url={http://dx.doi.org/10.1016/j.jmb.2004.12.044}, DOI={10.1016/j.jmb.2004.12.044}, number={5}, journal={Journal of Molecular Biology}, publisher={Elsevier BV}, author={Musayev, Faik and Sachdeva, Sarbjot and Neel Scarsdale, J. and Reynolds, K.A. and Wright, H.T.}, year={2005}, month=mar, pages={1313–1321} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T20:24:18Z",
    "timestamp": 1754598258428
  },
  "reference-count": 31,
  "publisher": "Elsevier BV",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            1
          ]
        ],
        "date-time": "2005-03-01T00:00:00Z",
        "timestamp": 1109635200000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2005,
        3
      ]
    ]
  },
  "DOI": "10.1016/j.jmb.2004.12.044",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        1,
        6
      ]
    ],
    "date-time": "2005-01-06T12:15:12Z",
    "timestamp": 1105013712000
  },
  "page": "1313-1321",
  "source": "Crossref",
  "is-referenced-by-count": 52,
  "title": "Crystal Structure of a Substrate Complex of Mycobacterium tuberculosis \u03b2-Ketoacyl-acyl Carrier Protein Synthase III (FabH) with Lauroyl-coenzyme A",
  "prefix": "10.1016",
  "volume": "346",
  "author": [
    {
      "given": "Faik",
      "family": "Musayev",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Sarbjot",
      "family": "Sachdeva",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J.",
      "family": "Neel Scarsdale",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "K.A.",
      "family": "Reynolds",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "H.T.",
      "family": "Wright",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/j.jmb.2004.12.044_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "5588",
      "DOI": "10.1073/pnas.94.11.5588",
      "article-title": "Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities",
      "volume": "94",
      "author": "Chirala",
      "year": "1997",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib2",
      "series-title": "E.coli and Salmonella, Cellular and Molecular Biology",
      "first-page": "612",
      "article-title": "Biosynthesis of membrane lipids",
      "author": "Cronan",
      "year": "1996"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "84",
      "DOI": "10.1016/0076-6879(75)35141-0",
      "article-title": "Fatty acid synthases from Mycobacterium phlei",
      "volume": "35",
      "author": "Bloch",
      "year": "1975",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "143",
      "DOI": "10.1016/S0163-7827(98)00008-3",
      "article-title": "Mycolic acids: structure, biosynthesis and physiological functions",
      "volume": "37",
      "author": "Barry",
      "year": "1998",
      "journal-title": "Prog. Lipid Res."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "20516",
      "DOI": "10.1074/jbc.M010762200",
      "article-title": "Crystal structure of the Mycobacterium tuberculosis \u03b2-ketoacyl-acyl carrier protein synthase III",
      "volume": "276",
      "author": "Scarsdale",
      "year": "2001",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "185",
      "DOI": "10.1016/S0969-2126(00)00094-0",
      "article-title": "The 1.8\u00c5 crystal structure and active-site architecture of \u03b2-ketoacyl-acyl carrier protein synthase III (FabH) from Escherichia coli",
      "volume": "8",
      "author": "Davies",
      "year": "2000",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "341",
      "DOI": "10.1006/jmbi.2000.4457",
      "article-title": "Refined structures of \u03b2-ketoacyl-acyl carrier protein synthase III",
      "volume": "307",
      "author": "Qiu",
      "year": "2001",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "797",
      "DOI": "10.1016/S0969-2126(94)00081-6",
      "article-title": "The 2.8\u00c5 crystal structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: a five layered \u03b1\u03b2\u03b1\u03b2\u03b1 structure constructed from two core domains of identical topology",
      "volume": "2",
      "author": "Mathieu",
      "year": "1994",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "233",
      "DOI": "10.1016/S0969-2126(01)00583-4",
      "article-title": "Structures of \u03b2-ketoacyl-acyl carrier protein synthase I complexes with fatty acids elucidate its catalytic machinery",
      "volume": "9",
      "author": "Olsen",
      "year": "2001",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "1183",
      "DOI": "10.1093/emboj/17.5.1183",
      "article-title": "Crystal structure of \u03b2-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes",
      "volume": "17",
      "author": "Huang",
      "year": "1998",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "491",
      "DOI": "10.1006/jmbi.2000.4272",
      "article-title": "The crystal structure of \u03b2-ketoacyl-acyl carrier protein synthase II from Synechocystis sp. at 1.54\u00c5 resolution and its relationship to other condensing enzymes",
      "volume": "305",
      "author": "Moche",
      "year": "2001",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "775",
      "DOI": "10.1038/11553",
      "article-title": "Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis",
      "volume": "6",
      "author": "Ferrer",
      "year": "1999",
      "journal-title": "Nature Struct. Biol."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "919",
      "DOI": "10.1016/S1074-5521(00)00041-7",
      "article-title": "Structural control of polyketide formation in plant-specific polyketide synthases",
      "volume": "7",
      "author": "Jez",
      "year": "2000",
      "journal-title": "Chem. Biol."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "1559",
      "DOI": "10.1016/S0969-2126(02)00889-4",
      "article-title": "Crystal structure of the priming \u03b2-ketosynthase from the R1128 polyketide biosynthetic pathway",
      "volume": "10",
      "author": "Pan",
      "year": "2002",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "28201",
      "DOI": "10.1074/jbc.M003241200",
      "article-title": "Identification and substrate specificity of \u03b2-ketoacyl (acyl carrier protein) synthase III (mtFabH) from M.tuberculosis",
      "volume": "275",
      "author": "Choi",
      "year": "2000",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "4136",
      "DOI": "10.1128/JB.185.14.4136-4143.2003",
      "article-title": "The 1.3-Angstrom-resolution crystal structure of \u03b2-ketoacyl-acyl carrier protein synthase II from Streptococcus pneumoniae",
      "volume": "185",
      "author": "Price",
      "year": "2003",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "545",
      "DOI": "10.1007/BF00135313",
      "article-title": "HINT\u2014a new method of empirical hydrophobic field calculation for CoMFA",
      "volume": "5",
      "author": "Kellogg",
      "year": "1991",
      "journal-title": "J. Comput. Aided Mol. Des."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "760",
      "DOI": "10.1107/S0907444994003112",
      "article-title": "The CCP4 suite: programs for protein crystallography",
      "volume": "50",
      "year": "1994",
      "journal-title": "Acta Crystallog. sect. D"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib19",
      "series-title": "Proceedings of CCP4 Study Weekend on Recent Advances in Phasing",
      "article-title": "Scaling of MAD Data",
      "author": "Evans",
      "year": "1997"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib20",
      "series-title": "BioteX; A Suite of Programs for the Collection, Reduction, and Interpretation of Imaging Plate Data",
      "author": "Chen",
      "year": "1995"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "905",
      "DOI": "10.1107/S0907444998003254",
      "article-title": "Crystallography & NMR system: a new software suite for macromolecular structure determination",
      "volume": "54",
      "author": "Brunger",
      "year": "1998",
      "journal-title": "Acta Crystallog. sect. D"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "240",
      "DOI": "10.1107/S0907444996012255",
      "article-title": "Refinement of macromolecular structures by the maximum-likelihood method",
      "volume": "53",
      "author": "Murshudov",
      "year": "1997",
      "journal-title": "Acta Crystallog. sect. D"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "279",
      "DOI": "10.1107/S0021889887012755",
      "article-title": "Calculation of an omit map",
      "volume": "21",
      "author": "Bhat",
      "year": "1988",
      "journal-title": "J. Appl. Crystallog."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "110",
      "DOI": "10.1107/S0108767390010224",
      "article-title": "Improved methods for building protein models in electron density maps and the location of errors in these models",
      "volume": "47",
      "author": "Jones",
      "year": "1991",
      "journal-title": "Acta Crystallog. sect. A"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "174",
      "DOI": "10.1016/0263-7855(87)80024-3",
      "article-title": "TOM: a Frodo subpackage for protein-ligand fitting with interactive energy minimization",
      "volume": "5",
      "author": "Cambillau",
      "year": "1987",
      "journal-title": "J. Mol. Graph."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "156",
      "DOI": "10.1006/jsbi.1999.4094",
      "article-title": "XtalView Xfit: a versatile program for manipulating atomic coordinates and electron density",
      "volume": "125",
      "author": "McRee",
      "year": "1999",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "283",
      "DOI": "10.1107/S0021889892009944",
      "article-title": "Procheck: a program to check the stereochemical quality of protein structures",
      "volume": "26",
      "author": "Laskowski",
      "year": "1993",
      "journal-title": "J. Appl. Crystallog."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "272",
      "DOI": "10.1038/381272a0",
      "article-title": "Errors in protein structures",
      "volume": "381",
      "author": "Hooft",
      "year": "1996",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib29",
      "doi-asserted-by": "crossref",
      "first-page": "829",
      "DOI": "10.1107/S0907444996001783",
      "article-title": "Efficient rebuilding of protein structures",
      "volume": "52",
      "author": "Kleywegt",
      "year": "1996",
      "journal-title": "Acta Crystallog. sect. D"
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "1711",
      "DOI": "10.1006/jmbi.1998.2400",
      "article-title": "Visualizing and quantifiying molecular goodness of fit: small probe contact dots with explicit hydrogen atoms",
      "volume": "285",
      "author": "Word",
      "year": "1999",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "10.1016/j.jmb.2004.12.044_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "191",
      "DOI": "10.1107/S0907444998006684",
      "article-title": "SFCHECK: a unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model",
      "volume": "55",
      "author": "Vaguine",
      "year": "1999",
      "journal-title": "Acta Crystallog. sect. D"
    }
  ],
  "container-title": "Journal of Molecular Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0022283604016146?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0022283604016146?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        3
      ]
    ],
    "date-time": "2021-07-03T17:51:13Z",
    "timestamp": 1625334673000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S0022283604016146"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2005,
        3
      ]
    ]
  },
  "references-count": 31,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          2005,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "S0022283604016146"
  ],
  "URL": "http://dx.doi.org/10.1016/j.jmb.2004.12.044",
  "relation": {},
  "ISSN": [
    "0022-2836"
  ],
  "subject": [],
  "container-title-short": "Journal of Molecular Biology",
  "published": {
    "date-parts": [
      [
        2005,
        3
      ]
    ]
  }
}