DOI: 10.1016/j.jim.2005.10.019. Inclusion of Brefeldin A during dendritic cell isolation allows in vitro detection of cross-presented self-antigens

Inclusion of Brefeldin A during dendritic cell isolation allows in vitro detection of cross-presented self-antigens

10.1016/j.jim.2005.10.019

Crossref journal-article

Elsevier BV

Journal of Immunological Methods (78)

Authors 5

Dirk Benke (first)
Thilo Krüger (additional)
Andreas Lang (additional)
Emma E. Hamilton-Williams (additional)
Christian Kurts (additional)

References 29 Referenced 9

10.1084/jem.20020861 / J. Exp. Med. / The CD8a+ dendritic cell is responsible for inducing peripheral self-tolerance to tissue-associated antigens by Belz (2002)
10.1007/978-1-4757-9966-8_9 / Adv. Exp. Med. Biol. / Driving gene expression specifically in dendritic cells by Brocker (1997)
10.1016/S0092-8674(00)80724-2 / Cell / Brefeldin A: the advantage of being uncompetitive by Chardin (1999)
10.1084/jem.20020295 / J. Exp. Med. / Constitutive versus activation-dependent cross-presentation of immune complexes by CD8(+) and CD8(−) dendritic cells in vivo by den Haan (2002)
10.1084/jem.192.12.1685 / J. Exp. Med. / CD8(+) but not CD8(−) dendritic cells cross-prime cytotoxic T cells in vivo by den Haan (2000)
10.1002/eji.1830260904 / Eur. J. Immunol. / The functional half-life of H-2Kd-restricted T cell epitopes on living cells by Eberl (1996)
10.1016/0092-8674(94)90336-0 / Cell / MHC-dependent antigen processing and peptide presentation: providing ligands for T lymphocyte activation by Germain (1994)
10.4049/jimmunol.174.3.1159 / J. Immunol. / Cutting edge: TLR ligands are not sufficient to break cross-tolerance to self-antigens by Hamilton-Williams (2005)
10.1084/jem.194.6.769 / J. Exp. Med. / Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo by Hawiger (2001)
10.1038/35100512 / Nat. Rev. Immunol. / Cross-presentation in viral immunity and self-tolerance by Heath (2001)
10.1084/jem.194.6.707 / J. Exp. Med. / Phenotypic and functional analysis of CD8(+) T cells undergoing peripheral deletion in response to cross-presentation of self-antigen by Hernandez (2001)
10.1084/jem.20020161 / J. Exp. Med. / The CD8+ dendritic cell subset selectively endocytoses dying cells in culture and in vivo by Iyoda (2002)
10.1046/j.1523-1747.2000.00084.x / J. Invest. Dermatol. / Formation and kinetics of MHC class I–ovalbumin peptide complexes on immature and mature murine dendritic cells by Kukutsch (2000)
10.1084/jem.184.3.923 / J. Exp. Med. / Constitutive class I-restricted exogenous presentation of self antigens in vivo by Kurts (1996)
10.1084/jem.186.2.239 / J. Exp. Med. / Class I-restricted cross-presentation of exogenous self-antigens leads to deletion of autoreactive CD8(+) T cells by Kurts (1997)
10.4049/jimmunol.166.3.1439 / J. Immunol. / Dendritic cells are sufficient to cross-present self-antigens to CD8 T cells in vivo by Kurts (2001)
10.1038/ni978 / Nat. Immunol. / Cross-priming of CD8+ T cells stimulated by virus-induced type I interferon by Le Bon (2003)
10.4049/jimmunol.166.6.3678 / J. Immunol. / Rapid peptide turnover and inefficient presentation of exogenous antigen critically limit the activation of self-reactive CTL by dendritic cells by Ludewig (2001)
10.1084/jem.189.3.587 / J. Exp. Med. / CD8alpha+ and CD8alpha-subclasses of dendritic cells direct the development of distinct T helper cells in vivo by Maldonado-Lopez (1999)
10.1084/jem.20012023 / J. Exp. Med. / Rapid cytotoxic T lymphocyte activation occurs in the draining lymph nodes after cutaneous herpes simplex virus infection as a result of early antigen presentation and not the presence of virus by Mueller (2002)
10.4049/jimmunol.166.9.5327 / J. Immunol. / Cutting edge: intravenous soluble antigen is presented to CD4 T cells by CD8− dendritic cells, but cross-presented to CD8 T cells by CD8+ dendritic cells by Pooley (2001)
10.1084/jem.188.6.1075 / J. Exp. Med. / Predominant role for directly transfected dendritic cells in antigen presentation to CD8+ T cells after gene gun immunization by Porgador (1998)
10.1038/ni1165 / Nat. Immunol. / Resting dendritic cells induce peripheral CD8+ T cell tolerance through PD-1 and CTLA-4 by Probst (2005)
10.1073/pnas.231606698 / Proc. Natl. Acad. Sci. U. S. A. / Avoiding horror autotoxicus: the importance of dendritic cells in peripheral T cell tolerance by Steinman (2002)
10.1084/jem.191.3.411 / J. Exp. Med. / The induction of tolerance by dendritic cells that have captured apoptotic cells by Steinman (2000)
10.4049/jimmunol.145.3.812 / J. Immunol. / MHC class II-restricted presentation of native protein antigen by B cells is inhibitable by cycloheximide and brefeldin A by St-Pierre (1990)
10.4049/jimmunol.167.9.4869 / J. Immunol. / Stability of surface H-2K(b), H-2D(b), and peptide-receptive H-2K(b) on splenocytes by Su (2001)
10.1038/87730 / Nat. Immunol. / Naive CTLs require a single brief period of antigenic stimulation for clonal expansion and differentiation by van Stipdonk (2001)
10.1084/jem.176.1.47 / J. Exp. Med. / The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells by Vremec (1992)

Dates

Type	When
Created	19 years, 7 months ago (Jan. 20, 2006, 7:41 a.m.)
Deposited	2 years, 3 months ago (May 6, 2023, 3:13 a.m.)
Indexed	5 months ago (March 29, 2025, 12:18 p.m.)
Issued	19 years, 6 months ago (March 1, 2006)
Published	19 years, 6 months ago (March 1, 2006)
Published Print	19 years, 6 months ago (March 1, 2006)

Funders 0

None

BibTeX

@article{Benke_2006, title={Inclusion of Brefeldin A during dendritic cell isolation allows in vitro detection of cross-presented self-antigens}, volume={310}, ISSN={0022-1759}, url={http://dx.doi.org/10.1016/j.jim.2005.10.019}, DOI={10.1016/j.jim.2005.10.019}, number={1–2}, journal={Journal of Immunological Methods}, publisher={Elsevier BV}, author={Benke, Dirk and Krüger, Thilo and Lang, Andreas and Hamilton-Williams, Emma E. and Kurts, Christian}, year={2006}, month=mar, pages={12–19} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        3,
        29
      ]
    ],
    "date-time": "2025-03-29T16:18:07Z",
    "timestamp": 1743265087689
  },
  "reference-count": 29,
  "publisher": "Elsevier BV",
  "issue": "1-2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2006,
            3,
            1
          ]
        ],
        "date-time": "2006-03-01T00:00:00Z",
        "timestamp": 1141171200000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2006,
        3
      ]
    ]
  },
  "DOI": "10.1016/j.jim.2005.10.019",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        1,
        20
      ]
    ],
    "date-time": "2006-01-20T12:41:25Z",
    "timestamp": 1137760885000
  },
  "page": "12-19",
  "source": "Crossref",
  "is-referenced-by-count": 9,
  "title": "Inclusion of Brefeldin A during dendritic cell isolation allows in vitro detection of cross-presented self-antigens",
  "prefix": "10.1016",
  "volume": "310",
  "author": [
    {
      "given": "Dirk",
      "family": "Benke",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Thilo",
      "family": "Kr\u00fcger",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Andreas",
      "family": "Lang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Emma E.",
      "family": "Hamilton-Williams",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Christian",
      "family": "Kurts",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/j.jim.2005.10.019_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "1099",
      "DOI": "10.1084/jem.20020861",
      "article-title": "The CD8a+ dendritic cell is responsible for inducing peripheral self-tolerance to tissue-associated antigens",
      "volume": "196",
      "author": "Belz",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "55",
      "DOI": "10.1007/978-1-4757-9966-8_9",
      "article-title": "Driving gene expression specifically in dendritic cells",
      "volume": "417",
      "author": "Brocker",
      "year": "1997",
      "journal-title": "Adv. Exp. Med. Biol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "153",
      "DOI": "10.1016/S0092-8674(00)80724-2",
      "article-title": "Brefeldin A: the advantage of being uncompetitive",
      "volume": "97",
      "author": "Chardin",
      "year": "1999",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "817",
      "DOI": "10.1084/jem.20020295",
      "article-title": "Constitutive versus activation-dependent cross-presentation of immune complexes by CD8(+) and CD8(\u2212) dendritic cells in vivo",
      "volume": "196",
      "author": "den Haan",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "1685",
      "DOI": "10.1084/jem.192.12.1685",
      "article-title": "CD8(+) but not CD8(\u2212) dendritic cells cross-prime cytotoxic T cells in vivo",
      "volume": "192",
      "author": "den Haan",
      "year": "2000",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "1993",
      "DOI": "10.1002/eji.1830260904",
      "article-title": "The functional half-life of H-2Kd-restricted T cell epitopes on living cells",
      "volume": "26",
      "author": "Eberl",
      "year": "1996",
      "journal-title": "Eur. J. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "287",
      "DOI": "10.1016/0092-8674(94)90336-0",
      "article-title": "MHC-dependent antigen processing and peptide presentation: providing ligands for T lymphocyte activation",
      "volume": "76",
      "author": "Germain",
      "year": "1994",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "1159",
      "DOI": "10.4049/jimmunol.174.3.1159",
      "article-title": "Cutting edge: TLR ligands are not sufficient to break cross-tolerance to self-antigens",
      "volume": "174",
      "author": "Hamilton-Williams",
      "year": "2005",
      "journal-title": "J. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "769",
      "DOI": "10.1084/jem.194.6.769",
      "article-title": "Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo",
      "volume": "194",
      "author": "Hawiger",
      "year": "2001",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "126",
      "DOI": "10.1038/35100512",
      "article-title": "Cross-presentation in viral immunity and self-tolerance",
      "volume": "1",
      "author": "Heath",
      "year": "2001",
      "journal-title": "Nat. Rev. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "707",
      "DOI": "10.1084/jem.194.6.707",
      "article-title": "Phenotypic and functional analysis of CD8(+) T cells undergoing peripheral deletion in response to cross-presentation of self-antigen",
      "volume": "194",
      "author": "Hernandez",
      "year": "2001",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "1289",
      "DOI": "10.1084/jem.20020161",
      "article-title": "The CD8+ dendritic cell subset selectively endocytoses dying cells in culture and in vivo",
      "volume": "195",
      "author": "Iyoda",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "449",
      "DOI": "10.1046/j.1523-1747.2000.00084.x",
      "article-title": "Formation and kinetics of MHC class I\u2013ovalbumin peptide complexes on immature and mature murine dendritic cells",
      "volume": "115",
      "author": "Kukutsch",
      "year": "2000",
      "journal-title": "J. Invest. Dermatol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "923",
      "DOI": "10.1084/jem.184.3.923",
      "article-title": "Constitutive class I-restricted exogenous presentation of self antigens in vivo",
      "volume": "184",
      "author": "Kurts",
      "year": "1996",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "239",
      "DOI": "10.1084/jem.186.2.239",
      "article-title": "Class I-restricted cross-presentation of exogenous self-antigens leads to deletion of autoreactive CD8(+) T cells",
      "volume": "186",
      "author": "Kurts",
      "year": "1997",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "1439",
      "DOI": "10.4049/jimmunol.166.3.1439",
      "article-title": "Dendritic cells are sufficient to cross-present self-antigens to CD8 T cells in vivo",
      "volume": "166",
      "author": "Kurts",
      "year": "2001",
      "journal-title": "J. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "1009",
      "DOI": "10.1038/ni978",
      "article-title": "Cross-priming of CD8+ T cells stimulated by virus-induced type I interferon",
      "volume": "4",
      "author": "Le Bon",
      "year": "2003",
      "journal-title": "Nat. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "3678",
      "DOI": "10.4049/jimmunol.166.6.3678",
      "article-title": "Rapid peptide turnover and inefficient presentation of exogenous antigen critically limit the activation of self-reactive CTL by dendritic cells",
      "volume": "166",
      "author": "Ludewig",
      "year": "2001",
      "journal-title": "J. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib19",
      "doi-asserted-by": "crossref",
      "first-page": "587",
      "DOI": "10.1084/jem.189.3.587",
      "article-title": "CD8alpha+ and CD8alpha-subclasses of dendritic cells direct the development of distinct T helper cells in vivo",
      "volume": "189",
      "author": "Maldonado-Lopez",
      "year": "1999",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib20",
      "doi-asserted-by": "crossref",
      "first-page": "651",
      "DOI": "10.1084/jem.20012023",
      "article-title": "Rapid cytotoxic T lymphocyte activation occurs in the draining lymph nodes after cutaneous herpes simplex virus infection as a result of early antigen presentation and not the presence of virus",
      "volume": "195",
      "author": "Mueller",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "5327",
      "DOI": "10.4049/jimmunol.166.9.5327",
      "article-title": "Cutting edge: intravenous soluble antigen is presented to CD4 T cells by CD8\u2212 dendritic cells, but cross-presented to CD8 T cells by CD8+ dendritic cells",
      "volume": "166",
      "author": "Pooley",
      "year": "2001",
      "journal-title": "J. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "1075",
      "DOI": "10.1084/jem.188.6.1075",
      "article-title": "Predominant role for directly transfected dendritic cells in antigen presentation to CD8+ T cells after gene gun immunization",
      "volume": "188",
      "author": "Porgador",
      "year": "1998",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "280",
      "DOI": "10.1038/ni1165",
      "article-title": "Resting dendritic cells induce peripheral CD8+ T cell tolerance through PD-1 and CTLA-4",
      "volume": "6",
      "author": "Probst",
      "year": "2005",
      "journal-title": "Nat. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "351",
      "DOI": "10.1073/pnas.231606698",
      "article-title": "Avoiding horror autotoxicus: the importance of dendritic cells in peripheral T cell tolerance",
      "volume": "99",
      "author": "Steinman",
      "year": "2002",
      "journal-title": "Proc. Natl. Acad. Sci. U. S. A."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "411",
      "DOI": "10.1084/jem.191.3.411",
      "article-title": "The induction of tolerance by dendritic cells that have captured apoptotic cells",
      "volume": "191",
      "author": "Steinman",
      "year": "2000",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "812",
      "DOI": "10.4049/jimmunol.145.3.812",
      "article-title": "MHC class II-restricted presentation of native protein antigen by B cells is inhibitable by cycloheximide and brefeldin A",
      "volume": "145",
      "author": "St-Pierre",
      "year": "1990",
      "journal-title": "J. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "4869",
      "DOI": "10.4049/jimmunol.167.9.4869",
      "article-title": "Stability of surface H-2K(b), H-2D(b), and peptide-receptive H-2K(b) on splenocytes",
      "volume": "167",
      "author": "Su",
      "year": "2001",
      "journal-title": "J. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "423",
      "DOI": "10.1038/87730",
      "article-title": "Naive CTLs require a single brief period of antigenic stimulation for clonal expansion and differentiation",
      "volume": "2",
      "author": "van Stipdonk",
      "year": "2001",
      "journal-title": "Nat. Immunol."
    },
    {
      "key": "10.1016/j.jim.2005.10.019_bib29",
      "doi-asserted-by": "crossref",
      "first-page": "47",
      "DOI": "10.1084/jem.176.1.47",
      "article-title": "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells",
      "volume": "176",
      "author": "Vremec",
      "year": "1992",
      "journal-title": "J. Exp. Med."
    }
  ],
  "container-title": "Journal of Immunological Methods",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S002217590500431X?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S002217590500431X?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        5,
        6
      ]
    ],
    "date-time": "2023-05-06T07:13:50Z",
    "timestamp": 1683357230000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S002217590500431X"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2006,
        3
      ]
    ]
  },
  "references-count": 29,
  "journal-issue": {
    "issue": "1-2",
    "published-print": {
      "date-parts": [
        [
          2006,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "S002217590500431X"
  ],
  "URL": "http://dx.doi.org/10.1016/j.jim.2005.10.019",
  "relation": {},
  "ISSN": [
    "0022-1759"
  ],
  "subject": [],
  "container-title-short": "Journal of Immunological Methods",
  "published": {
    "date-parts": [
      [
        2006,
        3
      ]
    ]
  }
}