DOI: 10.1016/j.febslet.2006.10.005. PP2Cγ‐mediated S‐phase accumulation induced by the proteasome‐dependent degradation of p21<sup>WAF1/CIP1</sup>

PP2Cγ‐mediated S‐phase accumulation induced by the proteasome‐dependent degradation of p21WAF1/CIP1

10.1016/j.febslet.2006.10.005

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Serine/threonine phosphatases such as PP1, PP2A, and PP2B are well known to regulate the transition phase of the cell cycle. However, the function of PP2Cγ in cell cycle progression is still unclear. In the present study, we report the characterization of PP2Cγ in mammalian cells during the cell cycle. After release of synchronized cells from thymidine block, over‐expression of PP2Cγ led to accumulation in the S phase. The amount of endogenous p21WAF1/CIP1 protein was markedly reduced by the expression of PP2Cγ. The degradation of p21WAF1/CIP1 induced by PP2Cγ was mediated in a proteasome‐dependent manner. In addition, the phosphatase activity of PP2Cγ was capable of repressing the level of p21WAF1/CIP1 protein. Phosphorylation of Rb was also reduced in cells expressing PP2Cγ. Taken together, these results indicate that PP2Cγ‐induced S phase accumulation may be associated with proteasome‐directed p21WAF1/CIP1 degradation.

Authors 3

Eun-Jung Suh (first)
Tae-Yoon Kim (additional)
Sang Hoon Kim (additional)

References 23 Referenced 11

10.1016/S0304-3835(01)00561-4
10.1074/jbc.M006210200
10.1146/annurev.bi.58.070189.002321
{'key': 'e_1_2_6_5_1', 'first-page': '1', 'article-title': 'Protein phosphatases: recent progress', 'volume': '23', 'author': 'Shenolikar S.', 'year': '1991', 'journal-title': 'Adv. Second Messenger Phosphoprot. Res.'} / Adv. Second Messenger Phosphoprot. Res. / Protein phosphatases: recent progress by Shenolikar S. (1991)
10.1074/jbc.273.52.35282
10.1023/B:MOLE.0000043624.96006.eb
10.1101/gad.13.22.2946
10.1074/jbc.M202742200
10.2741/Berndt
10.1152/ajplung.2000.278.5.L1062
10.1091/mbc.E02-07-0376
10.1101/gad.13.1.87
10.1128/MCB.17.9.5485
10.1038/sj.onc.1202681
10.1128/MCB.17.8.4877
10.1038/sj.onc.1202681
10.1016/j.molcel.2004.11.011
10.1074/jbc.M310373200
10.1093/emboj/20.10.2367
10.1016/j.molcel.2005.09.003
10.1016/j.semcdb.2005.02.010
10.1074/jbc.M301774200
10.1021/bi051071j

Dates

Type	When
Created	18 years, 10 months ago (Oct. 20, 2006, 8:20 a.m.)
Deposited	1 year, 10 months ago (Oct. 12, 2023, 6:25 p.m.)
Indexed	3 months, 1 week ago (May 23, 2025, 12:26 a.m.)
Issued	18 years, 10 months ago (Oct. 12, 2006)
Published	18 years, 10 months ago (Oct. 12, 2006)
Published Online	18 years, 10 months ago (Oct. 12, 2006)
Published Print	18 years, 9 months ago (Nov. 13, 2006)

Funders 0

None

BibTeX

@article{Suh_2006, title={PP2Cγ‐mediated S‐phase accumulation induced by the proteasome‐dependent degradation of p21WAF1/CIP1}, volume={580}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/j.febslet.2006.10.005}, DOI={10.1016/j.febslet.2006.10.005}, number={26}, journal={FEBS Letters}, publisher={Wiley}, author={Suh, Eun-Jung and Kim, Tae-Yoon and Kim, Sang Hoon}, year={2006}, month=oct, pages={6100–6104} }

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