DOI: 10.1016/j.febslet.2006.04.097. Characterization of dimeric ATP synthase and cristae membrane ultrastructure from <i>Saccharomyces</i> and <i>Polytomella</i> mitochondria

Characterization of dimeric ATP synthase and cristae membrane ultrastructure from Saccharomyces and Polytomella mitochondria

10.1016/j.febslet.2006.04.097

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

There is increasing evidence now that F1F0 ATP synthase is arranged in dimers in the inner mitochondrial membrane of several organisms. The dimers are also considered to be the building blocks of oligomers. It was recently found that the monomers in beef and the alga Polytomella ATP synthase dimer make an angle of ∼40° and ∼70°, respectively. This arrangement is considered to induce a strong local bending of the membrane. To further understand the packing of dimers into oligomers we performed an electron microscopy analysis of ATP synthase dimers purified from Saccharomyces cerevisiae. Two types of dimers were found in which the angle between the monomers is either ∼90° or ∼35°. According to our interpretation, the wide‐angle dimers (70–90°) are “true‐dimers” whereas the small‐angle dimers (35–40°) rather are “pseudo‐dimers”, which represent breakdown products of two adjacent true dimers in the oligomer. Ultrathin sectioning of intact Polytomella mitochondria indicates that the inner mitochondrial or cristae membrane is folded into lamellae and tubuli. Oligomers of ATP synthase can arrange in a helical fashion in tubular‐shaped cristae membranes. These results strongly support the hypothesized role of ATP synthase oligomers in structural determination of the mitochondrial inner membrane.

Bibliography

Dudkina, N. V., Sunderhaus, S., Braun, H.-P., & Boekema, E. J. (2006). Characterization of dimeric ATP synthase and cristae membrane ultrastructure from Saccharomyces and Polytomella mitochondria. FEBS Letters, 580(14), 3427â3432. Portico.

Authors 4

Natalya V. Dudkina (first)
Stephanie Sunderhaus (additional)
Hans-Peter Braun (additional)
Egbert J. Boekema (additional)

References 28 Referenced 137

10.1126/science.286.5445.1700
10.1016/S0005-2728(00)00092-X
10.1038/370621a0
10.1016/S0959-440X(00)00147-0
Walker J.E. and Dickson V.K. The peripheral stalk of the mitochondrial ATP synthase. Biochim. Biophys. Acta (in press).
10.1093/emboj/17.24.7170
10.1093/emboj/21.3.221
10.1016/S0005-2728(02)00274-8
10.1007/s10863-005-4128-8
10.1093/emboj/19.8.1777
10.1104/pp.103.024620
10.1104/pp.102.018325
10.1023/B:PLAN.0000009274.19340.36
10.1016/j.febslet.2005.09.065
10.1042/BJ20040566
10.1083/jcb.108.6.2233
10.1006/jsbi.1999.4204
10.1126/science.1104808
10.1038/238435a0
10.1073/pnas.0503893102
{'key': 'e_1_2_6_22_1', 'first-page': '33', 'article-title': 'Isolation of yeast mitochondria', 'volume': '313', 'author': 'Meisinger C.', 'year': '2006', 'journal-title': 'Methods Mol. Biol.'} / Methods Mol. Biol. / Isolation of yeast mitochondria by Meisinger C. (2006)
10.1016/0003-2697(91)90094-A
10.1073/pnas.0408870102
10.1111/j.1432-1033.1992.tb17197.x
10.1016/j.bbabio.2004.12.010
10.1016/j.bbrc.2005.02.010
10.1007/BF01280286
10.1242/jcs.01074

Dates

Type	When
Created	19 years, 3 months ago (May 13, 2006, 10:21 a.m.)
Deposited	1 year, 10 months ago (Oct. 12, 2023, 7:12 a.m.)
Indexed	1 week, 2 days ago (Aug. 23, 2025, 9:54 p.m.)
Issued	19 years, 3 months ago (May 12, 2006)
Published	19 years, 3 months ago (May 12, 2006)
Published Online	19 years, 3 months ago (May 12, 2006)
Published Print	19 years, 2 months ago (June 12, 2006)

Funders 0

None

BibTeX

@article{Dudkina_2006, title={Characterization of dimeric ATP synthase and cristae membrane ultrastructure from Saccharomyces and Polytomella mitochondria}, volume={580}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/j.febslet.2006.04.097}, DOI={10.1016/j.febslet.2006.04.097}, number={14}, journal={FEBS Letters}, publisher={Wiley}, author={Dudkina, Natalya V. and Sunderhaus, Stephanie and Braun, Hans-Peter and Boekema, Egbert J.}, year={2006}, month=may, pages={3427–3432} }

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