DOI: 10.1016/j.febslet.2006.03.082. Visualizing cytoskeleton dynamics in mammalian cells using a humanized variant of monomeric red fluorescent protein

Visualizing cytoskeleton dynamics in mammalian cells using a humanized variant of monomeric red fluorescent protein

10.1016/j.febslet.2006.03.082

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Fluorescent proteins are versatile tools for live cell imaging studies. In particular, recent progress was achieved in the development of monomeric red fluorescent proteins (mRFPs) that show improved properties in respect to maturation and intracellular fluorescence. mRFPmars, a red fluorescent protein designed especially for the use in Dictyostelium, proved to be a brilliant label for different cytoskeletal elements. Here we report on the synthesis of a humanized version of a monomeric RFP, mRFPruby, which differs in sequence from mRFPmars in four amino acids and has a codon usage that is optimized for the application in mammalian cells. In order to demonstrate the usefulness of this new mRFP variant, mRFPruby fused to β‐actin was expressed in different mouse cell lines and used to visualize actin cytoskeleton dynamics by live cell microscopy.

Bibliography

Fischer, M., Haase, I., Wiesner, S., & MÃ¼ller-Taubenberger, A. (2006). Visualizing cytoskeleton dynamics in mammalian cells using a humanized variant of monomeric red fluorescent protein. FEBS Letters, 580(10), 2495â2502. Portico.

Authors 4

Markus Fischer (first)
Ilka Haase (additional)
Sebastian Wiesner (additional)
Annette Müller-Taubenberger (additional)

References 22 Referenced 21

{'key': 'e_1_2_8_2_1', 'first-page': 'S1', 'author': 'Miyawaki A.', 'year': '2003', 'journal-title': 'Nat. Cell Biol.'} / Nat. Cell Biol. by Miyawaki A. (2003)
10.1126/science.1082520
10.1146/annurev.biochem.67.1.509
10.1038/nrm976
10.1038/nmeth819
10.1073/pnas.97.22.11984
10.1021/bi002263b
10.1038/13657
10.2144/02333rr02
10.1073/pnas.082243699
10.1038/nature03481
10.1016/j.febslet.2004.09.084
10.1073/pnas.0408546102
10.1093/nar/28.1.292
Stueber D. Matile H. and Garotta G. (1990) Immunological Methods IV (Lefkovits I. and Pernis P. Eds.) pp. 121–125. (10.1016/B978-0-12-442704-4.50014-1)
{'key': 'e_1_2_8_17_1', 'first-page': '376', 'volume': '5', 'author': 'Bullock W.O.', 'year': '1987', 'journal-title': 'BioTechniques'} / BioTechniques by Bullock W.O. (1987)
10.1128/jb.110.1.171-178.1972
{'key': 'e_1_2_8_19_1', 'first-page': '90', 'volume-title': 'Analytical Ultracentrifugation in Biochemistry and Polymer Science', 'author': 'Laue T.M.', 'year': '1992'} / Analytical Ultracentrifugation in Biochemistry and Polymer Science by Laue T.M. (1992)
10.1242/jcs.111.12.1649
10.1038/35008607
10.1529/biophysj.104.045617
10.1073/pnas.98.2.462

Dates

Type	When
Created	19 years, 4 months ago (April 18, 2006, 3:18 p.m.)
Deposited	1 year, 10 months ago (Oct. 12, 2023, 11:05 a.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 9:19 a.m.)
Issued	19 years, 4 months ago (April 17, 2006)
Published	19 years, 4 months ago (April 17, 2006)
Published Online	19 years, 4 months ago (April 17, 2006)
Published Print	19 years, 3 months ago (May 1, 2006)

Funders 0

None

BibTeX

@article{Fischer_2006, title={Visualizing cytoskeleton dynamics in mammalian cells using a humanized variant of monomeric red fluorescent protein}, volume={580}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/j.febslet.2006.03.082}, DOI={10.1016/j.febslet.2006.03.082}, number={10}, journal={FEBS Letters}, publisher={Wiley}, author={Fischer, Markus and Haase, Ilka and Wiesner, Sebastian and Müller-Taubenberger, Annette}, year={2006}, month=apr, pages={2495–2502} }

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