DOI: 10.1016/j.febslet.2004.08.086. Cooperative binding of the hnRNP K three KH domains to mRNA targets

Cooperative binding of the hnRNP K three KH domains to mRNA targets

10.1016/j.febslet.2004.08.086

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The heterogeneous nuclear ribonucleoprotein (hnRNP) K homology (KH) domain is an evolutionarily conserved module that binds short ribonucleotide sequences. KH domains most often are present in multiple copies per protein. In vitro studies of hnRNP K and other KH domain bearing proteins have yielded conflicting results regarding the relative contribution of each KH domain to the binding of target RNAs. To assess this RNA‐binding we used full‐length hnRNP K, its fragments and the yeast ortholog as baits in the yeast three‐hybrid system. The results demonstrate that in this heterologous in vivo system, the three KH domains bind RNA synergistically and that a single KH domain, in comparison, binds RNA weakly.

Bibliography

Paziewska, A., Wyrwicz, L. S., Bujnicki, J. M., Bomsztyk, K., & Ostrowski, J. (2004). Cooperative binding of the hnRNP K three KH domains to mRNA targets. FEBS Letters, 577(1â2), 134â140. Portico.

Authors 5

Agnieszka Paziewska (first)
Lucjan S. Wyrwicz (additional)
Janusz M. Bujnicki (additional)
Karol Bomsztyk (additional)
Jerzy Ostrowski (additional)

References 39 Referenced 59

Dates

Type	When
Created	20 years, 10 months ago (Oct. 14, 2004, 11:18 a.m.)
Deposited	1 year, 10 months ago (Oct. 12, 2023, 11:01 a.m.)
Indexed	1 day, 12 hours ago (Sept. 4, 2025, 10:06 a.m.)
Issued	20 years, 10 months ago (Oct. 13, 2004)
Published	20 years, 10 months ago (Oct. 13, 2004)
Published Online	20 years, 10 months ago (Oct. 13, 2004)
Published Print	20 years, 10 months ago (Nov. 5, 2004)

Funders 0

None

BibTeX

@article{Paziewska_2004, title={Cooperative binding of the hnRNP K three KH domains to mRNA targets}, volume={577}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/j.febslet.2004.08.086}, DOI={10.1016/j.febslet.2004.08.086}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Paziewska, Agnieszka and Wyrwicz, Lucjan S. and Bujnicki, Janusz M. and Bomsztyk, Karol and Ostrowski, Jerzy}, year={2004}, month=oct, pages={134–140} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T14:06:38Z",
    "timestamp": 1756994798197
  },
  "reference-count": 39,
  "publisher": "Wiley",
  "issue": "1-2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2004,
            10,
            13
          ]
        ],
        "date-time": "2004-10-13T00:00:00Z",
        "timestamp": 1097625600000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2004,
        11,
        5
      ]
    ]
  },
  "abstract": "<jats:p>The heterogeneous nuclear ribonucleoprotein (hnRNP) K homology (KH) domain is an evolutionarily conserved module that binds short ribonucleotide sequences. KH domains most often are present in multiple copies per protein. In vitro studies of hnRNP K and other KH domain bearing proteins have yielded conflicting results regarding the relative contribution of each KH domain to the binding of target RNAs. To assess this RNA\u2010binding we used full\u2010length hnRNP K, its fragments and the yeast ortholog as baits in the yeast three\u2010hybrid system. The results demonstrate that in this heterologous in vivo system, the three KH domains bind RNA synergistically and that a single KH domain, in comparison, binds RNA weakly.</jats:p>",
  "DOI": "10.1016/j.febslet.2004.08.086",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        10,
        14
      ]
    ],
    "date-time": "2004-10-14T15:18:48Z",
    "timestamp": 1097767128000
  },
  "page": "134-140",
  "source": "Crossref",
  "is-referenced-by-count": 59,
  "title": "Cooperative binding of the hnRNP K three KH domains to mRNA targets",
  "prefix": "10.1002",
  "volume": "577",
  "author": [
    {
      "given": "Agnieszka",
      "family": "Paziewska",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Lucjan S.",
      "family": "Wyrwicz",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Janusz M.",
      "family": "Bujnicki",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Karol",
      "family": "Bomsztyk",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jerzy",
      "family": "Ostrowski",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2004,
        10,
        13
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_5_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nrm760"
    },
    {
      "key": "e_1_2_5_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nrg1321"
    },
    {
      "key": "e_1_2_5_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35057062"
    },
    {
      "key": "e_1_2_5_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/29.3.638"
    },
    {
      "key": "e_1_2_5_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(93)80152-K"
    },
    {
      "key": "e_1_2_5_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1017/S1355838202024627"
    },
    {
      "key": "e_1_2_5_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1996.00425.x"
    },
    {
      "key": "e_1_2_5_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/22.1.53"
    },
    {
      "key": "e_1_2_5_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90420-U"
    },
    {
      "key": "e_1_2_5_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/21.5.1193"
    },
    {
      "key": "e_1_2_5_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M010594200"
    },
    {
      "key": "e_1_2_5_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90232-1"
    },
    {
      "key": "e_1_2_5_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M110267200"
    },
    {
      "key": "e_1_2_5_15_1",
      "unstructured": "Klimek-Tomczak K. Wyrwicz L.S. Jain S. Bomsztyk K. and Ostrowski J. (2004) J. Mol. Biol. (in press)"
    },
    {
      "key": "e_1_2_5_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)32487-0"
    },
    {
      "key": "e_1_2_5_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.22.1.286-297.2002"
    },
    {
      "key": "e_1_2_5_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/bies.20048"
    },
    {
      "key": "e_1_2_5_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00016a040"
    },
    {
      "key": "e_1_2_5_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1992.tb05331.x"
    },
    {
      "key": "e_1_2_5_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)00566-9"
    },
    {
      "key": "e_1_2_5_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1017/S1355838299990647"
    },
    {
      "key": "e_1_2_5_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1261/rna.5960503"
    },
    {
      "key": "e_1_2_5_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-5793(03)01434-0"
    },
    {
      "key": "e_1_2_5_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi000105e"
    },
    {
      "key": "e_1_2_5_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1999.2818"
    },
    {
      "key": "e_1_2_5_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-0282(1999)51:2<153::AID-BIP5>3.0.CO;2-6"
    },
    {
      "key": "e_1_2_5_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1371/journal.pbio.0000058"
    },
    {
      "key": "e_1_2_5_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/bioinformatics/17.8.750"
    },
    {
      "key": "e_1_2_5_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/bioinformatics/btg124"
    },
    {
      "key": "e_1_2_5_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/cdf352"
    },
    {
      "key": "e_1_2_5_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bbcan.2003.09.001"
    },
    {
      "key": "e_1_2_5_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1064719"
    },
    {
      "key": "e_1_2_5_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.43.27274"
    },
    {
      "key": "e_1_2_5_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.6.3129"
    },
    {
      "key": "e_1_2_5_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.17.10.5707"
    },
    {
      "key": "e_1_2_5_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsmb706"
    },
    {
      "key": "e_1_2_5_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444904002628"
    },
    {
      "key": "e_1_2_5_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80668-6"
    },
    {
      "key": "e_1_2_5_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/4151051a"
    }
  ],
  "container-title": "FEBS Letters",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2004.08.086",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2004.08.086",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        12
      ]
    ],
    "date-time": "2023-10-12T15:01:21Z",
    "timestamp": 1697122881000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1016/j.febslet.2004.08.086"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2004,
        10,
        13
      ]
    ]
  },
  "references-count": 39,
  "journal-issue": {
    "issue": "1-2",
    "published-print": {
      "date-parts": [
        [
          2004,
          11,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1016/j.febslet.2004.08.086"
  ],
  "URL": "http://dx.doi.org/10.1016/j.febslet.2004.08.086",
  "relation": {},
  "ISSN": [
    "0014-5793",
    "1873-3468"
  ],
  "subject": [],
  "container-title-short": "FEBS Letters",
  "published": {
    "date-parts": [
      [
        2004,
        10,
        13
      ]
    ]
  }
}