DOI: 10.1016/j.febslet.2004.07.035. Near‐field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells

Near‐field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells

10.1016/j.febslet.2004.07.035

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Clustering of cell surface receptors into micro‐domains in the plasma membrane is an important mechanism for regulating cellular functions. Unfortunately, these domains are often too small to be resolved with conventional optical microscopy. Near‐field scanning optical microscopy (NSOM) is a relatively new technique that combines ultra high optical resolution, down to 70 nm, with single molecule detection sensitivity. As such, the technique holds great potential for direct visualisation of domains at the cell surface. Yet, NSOM operation under liquid conditions is far from trivial. In this contribution, we show that the performance of NSOM can be extended to measurements in liquid environments using a diving bell concept. For the first time, individual fluorescent molecules on the membrane of cells in solution are imaged with a spatial resolution of 90 nm. Furthermore, using this technique we have been able to directly visualise nanometric sized domains of the C‐type lectin DC‐SIGN on the membrane of dendritic cells, both in air and in liquid.

Bibliography

Koopman, M., Cambi, A., de Bakker, B. I., Joosten, B., Figdor, C. G., van Hulst, N. F., & Garcia-Parajo, M. F. (2004). Nearâfield scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells. FEBS Letters, 573(1â3), 6â10. Portico.

Authors 7

M. Koopman (first)
A. Cambi (additional)
B.I. de Bakker (additional)
B. Joosten (additional)
C.G. Figdor (additional)
N.F. van Hulst (additional)
M.F. Garcia-Parajo (additional)

References 24 Referenced 95

10.1073/pnas.1332550100
10.1073/pnas.0438057100
10.1073/pnas.97.11.6013
10.1083/jcb.200306112
10.1126/science.1082160
{'key': 'e_1_2_6_7_1', 'first-page': 'SS1', 'author': 'Sako Y.', 'year': '2003', 'journal-title': 'Nature Cell Biol.'} / Nature Cell Biol. by Sako Y. (2003)
10.1093/emboj/19.5.892
10.1038/nbt895
10.1063/1.1571656
10.1126/science.262.5138.1422
10.1126/science.1081025
10.1073/pnas.94.2.520
10.1016/S0006-3495(98)77927-5
10.1242/jcs.114.22.4063
10.1242/jcs.114.23.4153
10.1083/jcb.100.5.1474
10.1063/1.1634385
10.1016/S0092-8674(00)80693-5
10.1016/S0092-8674(00)80694-7
10.1038/42408
10.1063/1.113340
10.1063/1.119482
10.1016/S0304-3991(97)00111-3
10.1016/S0021-9258(18)47340-1

Dates

Type	When
Created	21 years, 1 month ago (Aug. 4, 2004, 2:08 p.m.)
Deposited	1 year, 10 months ago (Oct. 12, 2023, 3:22 p.m.)
Indexed	1 year ago (Aug. 10, 2024, 1:11 a.m.)
Issued	21 years, 1 month ago (July 28, 2004)
Published	21 years, 1 month ago (July 28, 2004)
Published Online	21 years, 1 month ago (July 28, 2004)
Published Print	21 years ago (Aug. 27, 2004)

Funders 0

None

BibTeX

@article{Koopman_2004, title={Near‐field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells}, volume={573}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/j.febslet.2004.07.035}, DOI={10.1016/j.febslet.2004.07.035}, number={1–3}, journal={FEBS Letters}, publisher={Wiley}, author={Koopman, M. and Cambi, A. and de Bakker, B.I. and Joosten, B. and Figdor, C.G. and van Hulst, N.F. and Garcia-Parajo, M.F.}, year={2004}, month=jul, pages={6–10} }

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