DOI: 10.1016/j.febslet.2004.05.067. The α4 and α7 subunits and assembly of the 20S proteasome

The α4 and α7 subunits and assembly of the 20S proteasome

10.1016/j.febslet.2004.05.067

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The detailed mechanism of eukaryotic 20S proteasome assembly is currently unknown. In the present study, we demonstrate that the 20S proteasome subunits α4 and α7 interact with each other as well as all the α‐subunits in vivo and in vitro. The N‐terminal parts of α4 and α7 are essential for these newly discovered interactions in vitro. Glycerol gradient centrifugation of soluble extracts of HEK293 cells and Western blot analyses show that several α‐subunits are found in non‐proteasomal low‐density fractions. The α4 and α7 subunits co‐immunoprecipitate together from these low‐density fractions. The unexpected interaction between α4 and α7 may provide a molecular basis for the formation of previously reported 13S and 16S assembly intermediates.

Bibliography

Apcher, G.-S., Maitland, J., Dawson, S., Sheppard, P., & Mayer, R. J. (2004). The Î±4 and Î±7 subunits and assembly of the 20S proteasome. FEBS Letters, 569(1â3), 211â216. Portico.

Authors 5

Géraud-Sébastien Apcher (first)
Joanne Maitland (additional)
Simon Dawson (additional)
Paul Sheppard (additional)
R.John Mayer (additional)

References 28 Referenced 22

Dates

Type	When
Created	21 years, 2 months ago (June 11, 2004, 8:26 a.m.)
Deposited	1 year, 10 months ago (Oct. 12, 2023, 10:30 p.m.)
Indexed	4 months, 4 weeks ago (March 30, 2025, 6:04 p.m.)
Issued	21 years, 2 months ago (June 9, 2004)
Published	21 years, 2 months ago (June 9, 2004)
Published Online	21 years, 2 months ago (June 9, 2004)
Published Print	21 years, 1 month ago (July 2, 2004)

Funders 0

None

BibTeX

@article{Apcher_2004, title={The α4 and α7 subunits and assembly of the 20S proteasome}, volume={569}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/j.febslet.2004.05.067}, DOI={10.1016/j.febslet.2004.05.067}, number={1–3}, journal={FEBS Letters}, publisher={Wiley}, author={Apcher, Géraud-Sébastien and Maitland, Joanne and Dawson, Simon and Sheppard, Paul and Mayer, R.John}, year={2004}, month=jun, pages={211–216} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        3,
        30
      ]
    ],
    "date-time": "2025-03-30T22:04:02Z",
    "timestamp": 1743372242702
  },
  "reference-count": 28,
  "publisher": "Wiley",
  "issue": "1-3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2004,
            6,
            9
          ]
        ],
        "date-time": "2004-06-09T00:00:00Z",
        "timestamp": 1086739200000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2004,
        7,
        2
      ]
    ]
  },
  "abstract": "<jats:p>The detailed mechanism of eukaryotic 20S proteasome assembly is currently unknown. In the present study, we demonstrate that the 20S proteasome subunits \u03b14 and \u03b17 interact with each other as well as all the \u03b1\u2010subunits in vivo and in vitro. The N\u2010terminal parts of \u03b14 and \u03b17 are essential for these newly discovered interactions in vitro. Glycerol gradient centrifugation of soluble extracts of HEK293 cells and Western blot analyses show that several \u03b1\u2010subunits are found in non\u2010proteasomal low\u2010density fractions. The \u03b14 and \u03b17 subunits co\u2010immunoprecipitate together from these low\u2010density fractions. The unexpected interaction between \u03b14 and \u03b17 may provide a molecular basis for the formation of previously reported 13S and 16S assembly intermediates.</jats:p>",
  "DOI": "10.1016/j.febslet.2004.05.067",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        6,
        11
      ]
    ],
    "date-time": "2004-06-11T12:26:53Z",
    "timestamp": 1086956813000
  },
  "page": "211-216",
  "source": "Crossref",
  "is-referenced-by-count": 22,
  "title": "The \u03b14 and \u03b17 subunits and assembly of the 20S proteasome",
  "prefix": "10.1002",
  "volume": "569",
  "author": [
    {
      "given": "G\u00e9raud-S\u00e9bastien",
      "family": "Apcher",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Joanne",
      "family": "Maitland",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Simon",
      "family": "Dawson",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Paul",
      "family": "Sheppard",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "R.John",
      "family": "Mayer",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2004,
        6,
        9
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90396-4"
    },
    {
      "key": "e_1_2_6_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj3450001"
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35040607"
    },
    {
      "key": "e_1_2_6_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.46.27687"
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(94)90003-5"
    },
    {
      "key": "e_1_2_6_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.17.5363"
    },
    {
      "key": "e_1_2_6_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb01081.x"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1096/fasebj.11.14.9409542"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80942-3"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.2000.3959"
    },
    {
      "key": "e_1_2_6_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/80992"
    },
    {
      "key": "e_1_2_6_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/386463a0"
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1997.1429"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/0264-6021:3440349"
    },
    {
      "key": "e_1_2_6_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.15.10080"
    },
    {
      "key": "e_1_2_6_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/340245a0"
    },
    {
      "key": "e_1_2_6_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M107313200"
    },
    {
      "key": "e_1_2_6_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(91)80686-W"
    },
    {
      "key": "e_1_2_6_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-5793(03)01025-1"
    },
    {
      "key": "e_1_2_6_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.70.8.5582-5591.1996"
    },
    {
      "key": "e_1_2_6_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj20031122"
    },
    {
      "key": "e_1_2_6_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1023/A:1006965602142"
    },
    {
      "key": "e_1_2_6_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bbrc.2003.12.119"
    },
    {
      "key": "e_1_2_6_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/embo-reports/kve184"
    },
    {
      "key": "e_1_2_6_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.emboj.7600059"
    },
    {
      "key": "e_1_2_6_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/SQB.1995.060.01.055"
    },
    {
      "key": "e_1_2_6_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsb1194-765"
    },
    {
      "key": "e_1_2_6_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0969-2126(02)00748-7"
    }
  ],
  "container-title": "FEBS Letters",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2004.05.067",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2004.05.067",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        13
      ]
    ],
    "date-time": "2023-10-13T02:30:38Z",
    "timestamp": 1697164238000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1016/j.febslet.2004.05.067"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2004,
        6,
        9
      ]
    ]
  },
  "references-count": 28,
  "journal-issue": {
    "issue": "1-3",
    "published-print": {
      "date-parts": [
        [
          2004,
          7,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1016/j.febslet.2004.05.067"
  ],
  "URL": "http://dx.doi.org/10.1016/j.febslet.2004.05.067",
  "relation": {},
  "ISSN": [
    "0014-5793",
    "1873-3468"
  ],
  "subject": [],
  "container-title-short": "FEBS Letters",
  "published": {
    "date-parts": [
      [
        2004,
        6,
        9
      ]
    ]
  }
}