DOI: 10.1016/j.febslet.2004.03.125. RACK1 inhibits the serum‐ and anchorage‐independent growth of v‐Src transformed cells

RACK1 inhibits the serum‐ and anchorage‐independent growth of v‐Src transformed cells

10.1016/j.febslet.2004.03.125

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Cancer cells are capable of serum‐ and anchorage‐independent growth, and focus formation on monolayers of normal cells. Previously, we showed that RACK1 inhibits c‐Src kinase activity and NIH3T3 cell growth. Here, we show that RACK1 partially inhibits v‐Src kinase activity, and the serum‐ and anchorage‐independent growth of v‐Src transformed cells, but has no effect on focus formation. RACK1‐overexpressing v‐Src cells show disassembly of podosomes, which are actin‐rich structures that are distinctive to fully transformed cells. Together, our results demonstrate that RACK1 overexpression in v‐Src cells partially reverses the transformed phenotype of the cells. Our results identify an endogenous inhibitor of the oncogenic Src tyrosine kinase and of cell transformation.

Bibliography

Mamidipudi, V., Chang, B. Y., Harte, R. A., Lee, K. C., & Cartwright, C. A. (2004). RACK1 inhibits the serumâ and anchorageâindependent growth of vâSrc transformed cells. FEBS Letters, 567(2â3), 321â326. Portico.

Authors 5

Vidya Mamidipudi (first)
Betty Y. Chang (additional)
Rachel A. Harte (additional)
Kelly C. Lee (additional)
Christine A. Cartwright (additional)

References 37 Referenced 20

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Dates

Type	When
Created	21 years, 3 months ago (May 15, 2004, 6:35 a.m.)
Deposited	1 year, 11 months ago (Sept. 17, 2023, 2:32 a.m.)
Indexed	1 year, 5 months ago (March 5, 2024, 3:24 p.m.)
Issued	21 years, 3 months ago (May 13, 2004)
Published	21 years, 3 months ago (May 13, 2004)
Published Online	21 years, 3 months ago (May 13, 2004)
Published Print	21 years, 3 months ago (June 4, 2004)

Funders 0

None

BibTeX

@article{Mamidipudi_2004, title={RACK1 inhibits the serum‐ and anchorage‐independent growth of v‐Src transformed cells}, volume={567}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/j.febslet.2004.03.125}, DOI={10.1016/j.febslet.2004.03.125}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Mamidipudi, Vidya and Chang, Betty Y. and Harte, Rachel A. and Lee, Kelly C. and Cartwright, Christine A.}, year={2004}, month=may, pages={321–326} }

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