DOI: 10.1016/j.cell.2015.01.037. Structure of the Type VI Secretion System Contractile Sheath

Structure of the Type VI Secretion System Contractile Sheath

10.1016/j.cell.2015.01.037

Crossref journal-article

Elsevier BV

Cell (78)

Bibliography

Kudryashev, M., Wang, R. Y.-R., Brackmann, M., Scherer, S., Maier, T., Baker, D., DiMaio, F., Stahlberg, H., Egelman, E. H., & Basler, M. (2015). Structure of the Type VI Secretion System Contractile Sheath. Cell, 160(5), 952â962.

Authors 10

Mikhail Kudryashev (first)
Ray Yu-Ruei Wang (additional)
Maximilian Brackmann (additional)
Sebastian Scherer (additional)
Timm Maier (additional)
David Baker (additional)
Frank DiMaio (additional)
Henning Stahlberg (additional)
Edward H. Egelman (additional)
Marek Basler (additional)

References 62 Referenced 224

10.1038/emboj.2009.36 / EMBO J. / The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria by Aksyuk (2009)
10.1016/j.str.2009.04.005 / Structure / The structure of gene product 6 of bacteriophage T4, the hinge-pin of the baseplate by Aksyuk (2009)
10.1016/j.str.2011.09.012 / Structure / Structural conservation of the myoviridae phage tail sheath protein fold by Aksyuk (2011)
10.1016/j.cell.2014.03.053 / Cell / High-resolution microtubule structures reveal the structural transitions in αβ-tubulin upon GTP hydrolysis by Alushin (2014)
10.1126/science.1249410 / Science / Structure of the yeast mitochondrial large ribosomal subunit by Amunts (2014)
10.1126/science.1222901 / Science / Type 6 secretion dynamics within and between bacterial cells by Basler (2012)
10.1038/nature10846 / Nature / Type VI secretion requires a dynamic contractile phage tail-like structure by Basler (2012)
10.1016/j.cell.2013.01.042 / Cell / Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions by Basler (2013)
10.1128/IAI.69.7.4681-4685.2001 / Infect. Immun. / Vibrio cholerae tolC is required for bile resistance and colonization by Bina (2001)
10.1038/emboj.2008.269 / EMBO J. / Remodelling of VipA/VipB tubules by ClpV-mediated threading is crucial for type VI protein secretion by Bönemann (2009)
10.1016/j.celrep.2012.11.027 / Cell Rep. / Imaging type VI secretion-mediated bacterial killing by Brunet (2013)
10.1002/embr.201337936 / EMBO Rep. / Type VI secretion and bacteriophage tail tubes share a common assembly pathway by Brunet (2014)
10.1016/j.jsb.2011.12.017 / J. Struct. Biol. / Dynamo: a flexible, user-friendly development tool for subtomogram averaging of cryo-EM data in high-performance computing environments by Castaño-Díez (2012)
10.1007/s00249-010-0648-1 / Eur. Biophys. J. / Fast uncoiling kinetics of F1C pili expressed by uropathogenic Escherichia coli are revealed on a single pilus level using force-measuring optical tweezers by Castelain (2011)
10.1107/S0907444909042073 / Acta Crystallogr. D Biol. Crystallogr. / MolProbity: all-atom structure validation for macromolecular crystallography by Chen (2010)
10.1093/nar/gkn238 / Nucleic Acids Res. / The Jpred 3 secondary structure prediction server by Cole (2008)
10.1107/S0907444906022116 / Acta Crystallogr. D Biol. Crystallogr. / The Buccaneer software for automated model building. 1. Tracing protein chains by Cowtan (2006)
10.1016/j.molcel.2006.07.004 / Mol. Cell / Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions by Craig (2006)
10.1016/j.jsb.2013.11.003 / J. Struct. Biol. / SPRING - an image processing package for single-particle based helical reconstruction from electron cryomicrographs by Desfosses (2014)
10.1038/nmeth.3286 / Nat. Methods / Atomic- accuracy models from 4.5-Å cryo-electron microscopy data with density-guided iterative local refinement by DiMaio (2015)
10.1016/S0304-3991(00)00062-0 / Ultramicroscopy / A robust algorithm for the reconstruction of helical filaments using single-particle methods by Egelman (2000)
10.1016/S0076-6879(10)82006-3 / Methods Enzymol. / Reconstruction of helical filaments and tubes by Egelman (2010)
10.1107/S0907444910007493 / Acta Crystallogr. D Biol. Crystallogr. / Features and development of Coot by Emsley (2010)
10.1016/S0304-3991(02)00336-4 / Ultramicroscopy / Evaluation of a hybrid pixel detector for electron microscopy by Faruqi (2003)
10.1016/j.jmb.2013.02.012 / J. Mol. Biol. / The molecular architecture of the bacteriophage T4 neck by Fokine (2013)
10.1006/jsbi.1996.0030 / J. Struct. Biol. / SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields by Frank (1996)
10.1016/j.str.2014.11.006 / Structure / Near-atomic resolution for one state of f-actin by Galkin (2015)
10.1128/jb.177.14.4121-4130.1995 / J. Bacteriol. / Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter by Guzman (1995)
10.1186/1471-2105-15-85 / BMC Bioinformatics / FreeContact: fast and free software for protein contact prediction from residue co-evolution by Kaján (2014)
10.1111/mmi.12147 / Mol. Microbiol. / ClpV recycles VipA/VipB tubules and prevents non-productive tubule formation to ensure efficient type VI protein secretion by Kapitein (2013)
10.1038/nsmb975 / Nat. Struct. Mol. Biol. / The tail structure of bacteriophage T4 and its mechanism of contraction by Kostyuchenko (2005)
10.1016/j.jmb.2007.05.022 / J. Mol. Biol. / Inference of macromolecular assemblies from crystalline state by Krissinel (2007)
10.1016/j.celrep.2014.05.034 / Cell Rep. / Structure of the VipA/B type VI Secretion complex suggests a contraction-state-specific recycling mechanism by Kube (2014)
10.1126/science.1251652 / Science / Biochemistry. The resolution revolution by Kühlbrandt (2014)
10.1007/978-1-4614-0980-9_5 / Adv. Exp. Med. Biol. / Contractile tail machines of bacteriophages by Leiman (2012)
10.1016/j.cell.2004.07.022 / Cell / Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host by Leiman (2004)
10.1073/pnas.0813360106 / Proc. Natl. Acad. Sci. USA / Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin by Leiman (2009)
10.1038/nmeth.2472 / Nat. Methods / Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM by Li (2013)
10.1099/mic.0.051987-0 / Microbiology / Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa by Lossi (2011)
10.1074/jbc.M112.439273 / J. Biol. Chem. / The HsiB1C1 (TssB-TssC) complex of the Pseudomonas aeruginosa type VI secretion system forms a bacteriophage tail sheathlike structure by Lossi (2013)
10.1016/j.cell.2014.02.008 / Cell / Unified polymerization mechanism for the assembly of ASC-dependent inflammasomes by Lu (2014)
10.1006/plas.1996.0001 / Plasmid / Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria by Metcalf (1996)
10.1038/328834a0 / Nature / The accessible surface area and stability of oligomeric proteins by Miller (1987)
10.1529/biophysj.106.088989 / Biophys. J. / The mechanical properties of E. coli type 1 pili measured by atomic force microscopy techniques by Miller (2006)
10.1016/S1047-8477(03)00069-8 / J. Struct. Biol. / Accurate determination of local defocus and specimen tilt in electron microscopy by Mindell (2003)
10.7554/eLife.02030 / eLife / Robust and accurate prediction of residue-residue interactions across protein interfaces using evolutionary information by Ovchinnikov (2014)
10.1002/jcc.20084 / J. Comput. Chem. / UCSF Chimera—a visualization system for exploratory research and analysis by Pettersen (2004)
10.1074/jbc.M111.253377 / J. Biol. Chem. / Molecular basis for the unique role of the AAA+ chaperone ClpV in type VI protein secretion by Pietrosiuk (2011)
10.1073/pnas.0706532104 / Proc. Natl. Acad. Sci. USA / Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin by Pukatzki (2007)
10.1016/j.jsb.2014.03.016 / J. Struct. Biol. / 2dx_automator: implementation of a semiautomatic high-throughput high-resolution cryo-electron crystallography pipeline by Scherer (2014)
10.1038/nmeth.2019 / Nat. Methods / Fiji: an open-source platform for biological-image analysis by Schindelin (2012)
10.1038/nature12453 / Nature / PAAR-repeat proteins sharpen and diversify the type VI secretion system spike by Shneider (2013)
10.1016/j.str.2013.08.005 / Structure / High-resolution comparative modeling with RosettaCM by Song (2013)
10.1016/j.jsb.2006.05.009 / J. Struct. Biol. / EMAN2: an extensible image processing suite for electron microscopy by Tang (2007)
{'key': '10.1016/j.cell.2015.01.037_bib55', 'article-title': 'Structure of the F-actin—tropomyosin complex', 'author': 'von der Ecken', 'year': '2014', 'journal-title': 'Nature'} / Nature / Structure of the F-actin—tropomyosin complex by von der Ecken (2014)
10.1016/j.cell.2014.05.024 / Cell / Structure of the mammalian ribosome-Sec61 complex to 3.4 Å resolution by Voorhees (2014)
10.1038/nrmicro2220 / Nat. Rev. Microbiol. / Structural biology of the chaperone-usher pathway of pilus biogenesis by Waksman (2009)
10.1038/srep01448 / Sci. Rep. / Protein structure alignment beyond spatial proximity by Wang (2013)
{'key': '10.1016/j.cell.2015.01.037_bib59', 'article-title': 'De novo protein structure determination from near-atomic-resolution cryo-EM maps', 'author': 'Wang', 'year': '2015', 'journal-title': 'Nat. Methods'} / Nat. Methods / De novo protein structure determination from near-atomic-resolution cryo-EM maps by Wang (2015)
10.1038/nature01830 / Nature / Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy by Yonekura (2003)
10.1126/science.1068539 / Science / Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells by Zacharias (2002)
10.1074/jbc.M113.499772 / J. Biol. Chem. / TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system by Zoued (2013)

Dates

Type	When
Created	10 years, 5 months ago (Feb. 26, 2015, 12:01 p.m.)
Deposited	6 years, 10 months ago (Sept. 24, 2018, 4:58 p.m.)
Indexed	3 weeks, 5 days ago (July 26, 2025, 4:58 a.m.)
Issued	10 years, 6 months ago (Feb. 1, 2015)
Published	10 years, 6 months ago (Feb. 1, 2015)
Published Print	10 years, 6 months ago (Feb. 1, 2015)

Funders 0

None

BibTeX

@article{Kudryashev_2015, title={Structure of the Type VI Secretion System Contractile Sheath}, volume={160}, ISSN={0092-8674}, url={http://dx.doi.org/10.1016/j.cell.2015.01.037}, DOI={10.1016/j.cell.2015.01.037}, number={5}, journal={Cell}, publisher={Elsevier BV}, author={Kudryashev, Mikhail and Wang, Ray Yu-Ruei and Brackmann, Maximilian and Scherer, Sebastian and Maier, Timm and Baker, David and DiMaio, Frank and Stahlberg, Henning and Egelman, Edward H. and Basler, Marek}, year={2015}, month=feb, pages={952–962} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        26
      ]
    ],
    "date-time": "2025-07-26T08:58:21Z",
    "timestamp": 1753520301245
  },
  "reference-count": 62,
  "publisher": "Elsevier BV",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2015,
            2,
            1
          ]
        ],
        "date-time": "2015-02-01T00:00:00Z",
        "timestamp": 1422748800000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    },
    {
      "start": {
        "date-parts": [
          [
            2016,
            2,
            26
          ]
        ],
        "date-time": "2016-02-26T00:00:00Z",
        "timestamp": 1456444800000
      },
      "content-version": "vor",
      "delay-in-days": 390,
      "URL": "https://www.elsevier.com/open-access/userlicense/1.0/"
    }
  ],
  "content-domain": {
    "domain": [
      "cell.com",
      "elsevier.com",
      "sciencedirect.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2015,
        2
      ]
    ]
  },
  "DOI": "10.1016/j.cell.2015.01.037",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        2,
        26
      ]
    ],
    "date-time": "2015-02-26T17:01:00Z",
    "timestamp": 1424970060000
  },
  "page": "952-962",
  "update-policy": "http://dx.doi.org/10.1016/elsevier_cm_policy",
  "source": "Crossref",
  "is-referenced-by-count": 224,
  "title": "Structure of the Type VI Secretion System Contractile Sheath",
  "prefix": "10.1016",
  "volume": "160",
  "author": [
    {
      "given": "Mikhail",
      "family": "Kudryashev",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Ray\u00a0Yu-Ruei",
      "family": "Wang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Maximilian",
      "family": "Brackmann",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Sebastian",
      "family": "Scherer",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Timm",
      "family": "Maier",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "David",
      "family": "Baker",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Frank",
      "family": "DiMaio",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Henning",
      "family": "Stahlberg",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Edward\u00a0H.",
      "family": "Egelman",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Marek",
      "family": "Basler",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/j.cell.2015.01.037_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "821",
      "DOI": "10.1038/emboj.2009.36",
      "article-title": "The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria",
      "volume": "28",
      "author": "Aksyuk",
      "year": "2009",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "800",
      "DOI": "10.1016/j.str.2009.04.005",
      "article-title": "The structure of gene product 6 of bacteriophage T4, the hinge-pin of the baseplate",
      "volume": "17",
      "author": "Aksyuk",
      "year": "2009",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "1885",
      "DOI": "10.1016/j.str.2011.09.012",
      "article-title": "Structural conservation of the myoviridae phage tail sheath protein fold",
      "volume": "19",
      "author": "Aksyuk",
      "year": "2011",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "1117",
      "DOI": "10.1016/j.cell.2014.03.053",
      "article-title": "High-resolution microtubule structures reveal the structural transitions in \u03b1\u03b2-tubulin upon GTP hydrolysis",
      "volume": "157",
      "author": "Alushin",
      "year": "2014",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "1485",
      "DOI": "10.1126/science.1249410",
      "article-title": "Structure of the yeast mitochondrial large ribosomal subunit",
      "volume": "343",
      "author": "Amunts",
      "year": "2014",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "815",
      "DOI": "10.1126/science.1222901",
      "article-title": "Type 6 secretion dynamics within and between bacterial cells",
      "volume": "337",
      "author": "Basler",
      "year": "2012",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "182",
      "DOI": "10.1038/nature10846",
      "article-title": "Type VI secretion requires a dynamic contractile phage tail-like structure",
      "volume": "483",
      "author": "Basler",
      "year": "2012",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "884",
      "DOI": "10.1016/j.cell.2013.01.042",
      "article-title": "Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions",
      "volume": "152",
      "author": "Basler",
      "year": "2013",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "4681",
      "DOI": "10.1128/IAI.69.7.4681-4685.2001",
      "article-title": "Vibrio cholerae tolC is required for bile resistance and colonization",
      "volume": "69",
      "author": "Bina",
      "year": "2001",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "315",
      "DOI": "10.1038/emboj.2008.269",
      "article-title": "Remodelling of VipA/VipB tubules by ClpV-mediated threading is crucial for type VI protein secretion",
      "volume": "28",
      "author": "B\u00f6nemann",
      "year": "2009",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "36",
      "DOI": "10.1016/j.celrep.2012.11.027",
      "article-title": "Imaging type VI secretion-mediated bacterial killing",
      "volume": "3",
      "author": "Brunet",
      "year": "2013",
      "journal-title": "Cell Rep."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "315",
      "DOI": "10.1002/embr.201337936",
      "article-title": "Type VI secretion and bacteriophage tail tubes share a common assembly pathway",
      "volume": "15",
      "author": "Brunet",
      "year": "2014",
      "journal-title": "EMBO Rep."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "139",
      "DOI": "10.1016/j.jsb.2011.12.017",
      "article-title": "Dynamo: a flexible, user-friendly development tool for subtomogram averaging of cryo-EM data in high-performance computing environments",
      "volume": "178",
      "author": "Casta\u00f1o-D\u00edez",
      "year": "2012",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "305",
      "DOI": "10.1007/s00249-010-0648-1",
      "article-title": "Fast uncoiling kinetics of F1C pili expressed by uropathogenic Escherichia coli are revealed on a single pilus level using force-measuring optical tweezers",
      "volume": "40",
      "author": "Castelain",
      "year": "2011",
      "journal-title": "Eur. Biophys. J."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "12",
      "DOI": "10.1107/S0907444909042073",
      "article-title": "MolProbity: all-atom structure validation for macromolecular crystallography",
      "volume": "66",
      "author": "Chen",
      "year": "2010",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "W197",
      "DOI": "10.1093/nar/gkn238",
      "article-title": "The Jpred 3 secondary structure prediction server",
      "volume": "36",
      "author": "Cole",
      "year": "2008",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "1002",
      "DOI": "10.1107/S0907444906022116",
      "article-title": "The Buccaneer software for automated model building. 1. Tracing protein chains",
      "volume": "62",
      "author": "Cowtan",
      "year": "2006",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "651",
      "DOI": "10.1016/j.molcel.2006.07.004",
      "article-title": "Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions",
      "volume": "23",
      "author": "Craig",
      "year": "2006",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib19",
      "doi-asserted-by": "crossref",
      "first-page": "15",
      "DOI": "10.1016/j.jsb.2013.11.003",
      "article-title": "SPRING - an image processing package for single-particle based helical reconstruction from electron cryomicrographs",
      "volume": "185",
      "author": "Desfosses",
      "year": "2014",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib20",
      "doi-asserted-by": "crossref",
      "DOI": "10.1038/nmeth.3286",
      "article-title": "Atomic- accuracy models from 4.5-\u00c5 cryo-electron microscopy data with density-guided iterative local refinement",
      "author": "DiMaio",
      "year": "2015",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "225",
      "DOI": "10.1016/S0304-3991(00)00062-0",
      "article-title": "A robust algorithm for the reconstruction of helical filaments using single-particle methods",
      "volume": "85",
      "author": "Egelman",
      "year": "2000",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "167",
      "DOI": "10.1016/S0076-6879(10)82006-3",
      "article-title": "Reconstruction of helical filaments and tubes",
      "volume": "482",
      "author": "Egelman",
      "year": "2010",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "486",
      "DOI": "10.1107/S0907444910007493",
      "article-title": "Features and development of Coot",
      "volume": "66",
      "author": "Emsley",
      "year": "2010",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "263",
      "DOI": "10.1016/S0304-3991(02)00336-4",
      "article-title": "Evaluation of a hybrid pixel detector for electron microscopy",
      "volume": "94",
      "author": "Faruqi",
      "year": "2003",
      "journal-title": "Ultramicroscopy"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "1731",
      "DOI": "10.1016/j.jmb.2013.02.012",
      "article-title": "The molecular architecture of the bacteriophage T4 neck",
      "volume": "425",
      "author": "Fokine",
      "year": "2013",
      "journal-title": "J.\u00a0Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "190",
      "DOI": "10.1006/jsbi.1996.0030",
      "article-title": "SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields",
      "volume": "116",
      "author": "Frank",
      "year": "1996",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "173",
      "DOI": "10.1016/j.str.2014.11.006",
      "article-title": "Near-atomic resolution for one state of f-actin",
      "volume": "23",
      "author": "Galkin",
      "year": "2015",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "4121",
      "DOI": "10.1128/jb.177.14.4121-4130.1995",
      "article-title": "Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter",
      "volume": "177",
      "author": "Guzman",
      "year": "1995",
      "journal-title": "J.\u00a0Bacteriol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib29",
      "doi-asserted-by": "crossref",
      "first-page": "85",
      "DOI": "10.1186/1471-2105-15-85",
      "article-title": "FreeContact: fast and free software for protein contact prediction from residue co-evolution",
      "volume": "15",
      "author": "Kaj\u00e1n",
      "year": "2014",
      "journal-title": "BMC Bioinformatics"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "1013",
      "DOI": "10.1111/mmi.12147",
      "article-title": "ClpV recycles VipA/VipB tubules and prevents non-productive tubule formation to ensure efficient type VI protein secretion",
      "volume": "87",
      "author": "Kapitein",
      "year": "2013",
      "journal-title": "Mol. Microbiol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "810",
      "DOI": "10.1038/nsmb975",
      "article-title": "The tail structure of bacteriophage T4 and its mechanism of contraction",
      "volume": "12",
      "author": "Kostyuchenko",
      "year": "2005",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib32",
      "doi-asserted-by": "crossref",
      "first-page": "774",
      "DOI": "10.1016/j.jmb.2007.05.022",
      "article-title": "Inference of macromolecular assemblies from crystalline state",
      "volume": "372",
      "author": "Krissinel",
      "year": "2007",
      "journal-title": "J.\u00a0Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "20",
      "DOI": "10.1016/j.celrep.2014.05.034",
      "article-title": "Structure of the VipA/B type VI Secretion complex suggests a contraction-state-specific recycling mechanism",
      "volume": "8",
      "author": "Kube",
      "year": "2014",
      "journal-title": "Cell Rep."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "1443",
      "DOI": "10.1126/science.1251652",
      "article-title": "Biochemistry. The resolution revolution",
      "volume": "343",
      "author": "K\u00fchlbrandt",
      "year": "2014",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "93",
      "DOI": "10.1007/978-1-4614-0980-9_5",
      "article-title": "Contractile tail machines of bacteriophages",
      "volume": "726",
      "author": "Leiman",
      "year": "2012",
      "journal-title": "Adv. Exp. Med. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib36",
      "doi-asserted-by": "crossref",
      "first-page": "419",
      "DOI": "10.1016/j.cell.2004.07.022",
      "article-title": "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host",
      "volume": "118",
      "author": "Leiman",
      "year": "2004",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib37",
      "doi-asserted-by": "crossref",
      "first-page": "4154",
      "DOI": "10.1073/pnas.0813360106",
      "article-title": "Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin",
      "volume": "106",
      "author": "Leiman",
      "year": "2009",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib38",
      "doi-asserted-by": "crossref",
      "first-page": "584",
      "DOI": "10.1038/nmeth.2472",
      "article-title": "Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM",
      "volume": "10",
      "author": "Li",
      "year": "2013",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib39",
      "doi-asserted-by": "crossref",
      "first-page": "3292",
      "DOI": "10.1099/mic.0.051987-0",
      "article-title": "Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa",
      "volume": "157",
      "author": "Lossi",
      "year": "2011",
      "journal-title": "Microbiology"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib40",
      "doi-asserted-by": "crossref",
      "first-page": "7536",
      "DOI": "10.1074/jbc.M112.439273",
      "article-title": "The HsiB1C1 (TssB-TssC) complex of the Pseudomonas aeruginosa type VI secretion system forms a bacteriophage tail sheathlike structure",
      "volume": "288",
      "author": "Lossi",
      "year": "2013",
      "journal-title": "J.\u00a0Biol. Chem."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib41",
      "doi-asserted-by": "crossref",
      "first-page": "1193",
      "DOI": "10.1016/j.cell.2014.02.008",
      "article-title": "Unified polymerization mechanism for the assembly of ASC-dependent inflammasomes",
      "volume": "156",
      "author": "Lu",
      "year": "2014",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib42",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1006/plas.1996.0001",
      "article-title": "Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria",
      "volume": "35",
      "author": "Metcalf",
      "year": "1996",
      "journal-title": "Plasmid"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib43",
      "doi-asserted-by": "crossref",
      "first-page": "834",
      "DOI": "10.1038/328834a0",
      "article-title": "The accessible surface area and stability of oligomeric proteins",
      "volume": "328",
      "author": "Miller",
      "year": "1987",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib44",
      "doi-asserted-by": "crossref",
      "first-page": "3848",
      "DOI": "10.1529/biophysj.106.088989",
      "article-title": "The mechanical properties of E. coli type 1 pili measured by atomic force microscopy techniques",
      "volume": "91",
      "author": "Miller",
      "year": "2006",
      "journal-title": "Biophys. J."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib45",
      "doi-asserted-by": "crossref",
      "first-page": "334",
      "DOI": "10.1016/S1047-8477(03)00069-8",
      "article-title": "Accurate determination of local defocus and specimen tilt in electron microscopy",
      "volume": "142",
      "author": "Mindell",
      "year": "2003",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib46",
      "doi-asserted-by": "crossref",
      "first-page": "e02030",
      "DOI": "10.7554/eLife.02030",
      "article-title": "Robust and accurate prediction of residue-residue interactions across protein interfaces using evolutionary information",
      "volume": "3",
      "author": "Ovchinnikov",
      "year": "2014",
      "journal-title": "eLife"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib47",
      "doi-asserted-by": "crossref",
      "first-page": "1605",
      "DOI": "10.1002/jcc.20084",
      "article-title": "UCSF Chimera\u2014a visualization system for exploratory research and analysis",
      "volume": "25",
      "author": "Pettersen",
      "year": "2004",
      "journal-title": "J.\u00a0Comput. Chem."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib48",
      "doi-asserted-by": "crossref",
      "first-page": "30010",
      "DOI": "10.1074/jbc.M111.253377",
      "article-title": "Molecular basis for the unique role of the\u00a0AAA+ chaperone ClpV in type VI protein secretion",
      "volume": "286",
      "author": "Pietrosiuk",
      "year": "2011",
      "journal-title": "J.\u00a0Biol. Chem."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib49",
      "doi-asserted-by": "crossref",
      "first-page": "15508",
      "DOI": "10.1073/pnas.0706532104",
      "article-title": "Type VI secretion system translocates a phage tail spike-like protein into target\u00a0cells where it cross-links actin",
      "volume": "104",
      "author": "Pukatzki",
      "year": "2007",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib50",
      "doi-asserted-by": "crossref",
      "first-page": "302",
      "DOI": "10.1016/j.jsb.2014.03.016",
      "article-title": "2dx_automator: implementation of a semiautomatic high-throughput high-resolution cryo-electron crystallography pipeline",
      "volume": "186",
      "author": "Scherer",
      "year": "2014",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib51",
      "doi-asserted-by": "crossref",
      "first-page": "676",
      "DOI": "10.1038/nmeth.2019",
      "article-title": "Fiji: an\u00a0open-source platform for biological-image analysis",
      "volume": "9",
      "author": "Schindelin",
      "year": "2012",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib52",
      "doi-asserted-by": "crossref",
      "first-page": "350",
      "DOI": "10.1038/nature12453",
      "article-title": "PAAR-repeat proteins sharpen and diversify the type VI secretion system spike",
      "volume": "500",
      "author": "Shneider",
      "year": "2013",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib53",
      "doi-asserted-by": "crossref",
      "first-page": "1735",
      "DOI": "10.1016/j.str.2013.08.005",
      "article-title": "High-resolution comparative modeling with RosettaCM",
      "volume": "21",
      "author": "Song",
      "year": "2013",
      "journal-title": "Structure"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib54",
      "doi-asserted-by": "crossref",
      "first-page": "38",
      "DOI": "10.1016/j.jsb.2006.05.009",
      "article-title": "EMAN2: an extensible image processing suite for electron microscopy",
      "volume": "157",
      "author": "Tang",
      "year": "2007",
      "journal-title": "J.\u00a0Struct. Biol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib55",
      "article-title": "Structure of the F-actin\u2014tropomyosin complex",
      "author": "von der Ecken",
      "year": "2014",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib56",
      "doi-asserted-by": "crossref",
      "first-page": "1632",
      "DOI": "10.1016/j.cell.2014.05.024",
      "article-title": "Structure of the mammalian ribosome-Sec61 complex to 3.4\u00a0\u00c5 resolution",
      "volume": "157",
      "author": "Voorhees",
      "year": "2014",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib57",
      "doi-asserted-by": "crossref",
      "first-page": "765",
      "DOI": "10.1038/nrmicro2220",
      "article-title": "Structural biology of the chaperone-usher pathway of pilus biogenesis",
      "volume": "7",
      "author": "Waksman",
      "year": "2009",
      "journal-title": "Nat. Rev. Microbiol."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib58",
      "doi-asserted-by": "crossref",
      "first-page": "1448",
      "DOI": "10.1038/srep01448",
      "article-title": "Protein structure alignment beyond spatial proximity",
      "volume": "3",
      "author": "Wang",
      "year": "2013",
      "journal-title": "Sci. Rep."
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib59",
      "article-title": "De novo protein structure determination from near-atomic-resolution cryo-EM maps",
      "author": "Wang",
      "year": "2015",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib60",
      "doi-asserted-by": "crossref",
      "first-page": "643",
      "DOI": "10.1038/nature01830",
      "article-title": "Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy",
      "volume": "424",
      "author": "Yonekura",
      "year": "2003",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib61",
      "doi-asserted-by": "crossref",
      "first-page": "913",
      "DOI": "10.1126/science.1068539",
      "article-title": "Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells",
      "volume": "296",
      "author": "Zacharias",
      "year": "2002",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2015.01.037_bib62",
      "doi-asserted-by": "crossref",
      "first-page": "27031",
      "DOI": "10.1074/jbc.M113.499772",
      "article-title": "TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system",
      "volume": "288",
      "author": "Zoued",
      "year": "2013",
      "journal-title": "J.\u00a0Biol. Chem."
    }
  ],
  "container-title": "Cell",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S009286741500080X?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S009286741500080X?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2018,
        9,
        24
      ]
    ],
    "date-time": "2018-09-24T20:58:23Z",
    "timestamp": 1537822703000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S009286741500080X"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2015,
        2
      ]
    ]
  },
  "references-count": 62,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          2015,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "S009286741500080X"
  ],
  "URL": "http://dx.doi.org/10.1016/j.cell.2015.01.037",
  "relation": {},
  "ISSN": [
    "0092-8674"
  ],
  "subject": [],
  "container-title-short": "Cell",
  "published": {
    "date-parts": [
      [
        2015,
        2
      ]
    ]
  },
  "assertion": [
    {
      "value": "Elsevier",
      "name": "publisher",
      "label": "This article is maintained by"
    },
    {
      "value": "Structure of the Type VI Secretion System Contractile Sheath",
      "name": "articletitle",
      "label": "Article Title"
    },
    {
      "value": "Cell",
      "name": "journaltitle",
      "label": "Journal Title"
    },
    {
      "value": "https://doi.org/10.1016/j.cell.2015.01.037",
      "name": "articlelink",
      "label": "CrossRef DOI link to publisher maintained version"
    },
    {
      "value": "https://doi.org/10.1016/j.cell.2015.02.005",
      "name": "associatedlink",
      "label": "CrossRef DOI link to the associated document"
    },
    {
      "value": "article",
      "name": "content_type",
      "label": "Content Type"
    },
    {
      "value": "Copyright \u00a9 2015 Elsevier Inc. All rights reserved.",
      "name": "copyright",
      "label": "Copyright"
    }
  ]
}