DOI: 10.1016/j.cell.2014.11.049. Conformational Changes of Elongation Factor G on the Ribosome during tRNA Translocation

Conformational Changes of Elongation Factor G on the Ribosome during tRNA Translocation

10.1016/j.cell.2014.11.049

Crossref journal-article

Elsevier BV

Cell (78)

Bibliography

Lin, J., Gagnon, M. G., Bulkley, D., & Steitz, T. A. (2015). Conformational Changes of Elongation Factor G on the Ribosome during tRNA Translocation. Cell, 160(1â2), 219â227.

Authors 4

Jinzhong Lin (first)
Matthieu G. Gagnon (additional)
David Bulkley (additional)
Thomas A. Steitz (additional)

References 63 Referenced 122

10.1107/S0907444902016657 / Acta Crystallogr. D Biol. Crystallogr. / PHENIX: building new software for automated crystallographic structure determination by Adams (2002)
10.1073/pnas.95.11.6134 / Proc. Natl. Acad. Sci. USA / Visualization of elongation factor G on the Escherichia coli 70S ribosome: the mechanism of translocation by Agrawal (1998)
10.1038/10695 / Nat. Struct. Biol. / EF-G-dependent GTP hydrolysis induces translocation accompanied by large conformational changes in the 70S ribosome by Agrawal (1999)
10.1126/science.1175800 / Science / Formation of the first peptide bond: the structure of EF-P bound to the 70S ribosome by Blaha (2009)
10.1073/pnas.0403884101 / Proc. Natl. Acad. Sci. USA / tRNA dynamics on the ribosome during translation by Blanchard (2004)
10.1074/jbc.M407008200 / J. Biol. Chem. / Interaction of thiostrepton and elongation factor-G with the ribosomal protein L11-binding domain by Bowen (2005)
10.1261/rna.61206 / RNA / Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits by Brandi (2006)
10.1016/j.febslet.2012.07.040 / FEBS Lett. / Structural and functional characterization of the bacterial translocation inhibitor GE82832 by Brandi (2012)
10.1073/pnas.1311423110 / Proc. Natl. Acad. Sci. USA / Structure of the ribosome with elongation factor G trapped in the pretranslocation state by Brilot (2013)
10.1016/j.celrep.2013.12.024 / Cell Reports / The antibiotics dityromycin and GE82832 bind protein S12 and block EF-G-catalyzed translocation by Bulkley (2014)
10.1016/j.molcel.2011.03.024 / Mol. Cell / Single-molecule fluorescence measurements of ribosomal translocation dynamics by Chen (2011)
10.1038/nsmb.2567 / Nat. Struct. Mol. Biol. / Coordinated conformational and compositional dynamics drive ribosome translocation by Chen (2013)
10.1038/nsmb.2645 / Nat. Struct. Mol. Biol. / Structure of EF-G-ribosome complex in a pretranslocation state by Chen (2013)
10.1016/j.molcel.2007.01.027 / Mol. Cell / Structural basis for interaction of the ribosome with the switch regions of GTP-bound elongation factors by Connell (2007)
10.1016/j.molcel.2008.05.004 / Mol. Cell / Spontaneous intersubunit rotation in single ribosomes by Cornish (2008)
10.1016/S1097-2765(03)00275-2 / Mol. Cell / Ribosomal proteins S12 and S13 function as control elements for translocation of the mRNA:tRNA complex by Cukras (2003)
10.1021/bi970610k / Biochemistry / The conformational properties of elongation factor G and the mechanism of translocation by Czworkowski (1997)
10.1002/j.1460-2075.1994.tb06675.x / EMBO J. / The crystal structure of elongation factor G complexed with GDP, at 2.7 A resolution by Czworkowski (1994)
DeLano, W.L. (2006). The PyMOL molecular graphics system. http://wwwpymolorg.
10.1107/S0907444904019158 / Acta Crystallogr. D Biol. Crystallogr. / Coot: model-building tools for molecular graphics by Emsley (2004)
10.1038/nsmb.2011 / Nat. Struct. Mol. Biol. / mRNA translocation occurs during the second step of ribosomal intersubunit rotation by Ermolenko (2011)
10.1016/j.molcel.2008.03.012 / Mol. Cell / Coupling of ribosomal L1 stalk and tRNA dynamics during translation elongation by Fei (2008)
10.1038/35018597 / Nature / A ratchet-like inter-subunit reorganization of the ribosome during translocation by Frank (2000)
10.1126/science.1217443 / Science / Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome by Gagnon (2012)
10.1126/science.1253525 / Science / Crystal structure of elongation factor 4 bound to a clockwise ratcheted ribosome by Gagnon (2014)
10.1126/science.1179709 / Science / The structure of the ribosome with elongation factor G trapped in the posttranslocational state by Gao (2009)
10.1073/pnas.1218999109 / Proc. Natl. Acad. Sci. USA / Rotation of the head of the 30S ribosomal subunit during mRNA translocation by Guo (2012)
10.1016/j.febslet.2005.07.016 / FEBS Lett. / Crystal structure of a mutant elongation factor G trapped with a GTP analogue by Hansson (2005)
10.1002/embj.201387465 / EMBO J. / GTP hydrolysis by EF-G synchronizes tRNA movement on small and large ribosomal subunits by Holtkamp (2014)
10.1107/S0021889893005588 / J. Appl. Cryst. / Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants by Kabsch (1993)
10.1074/jbc.M002656200 / J. Biol. Chem. / Domain III of elongation factor G from Thermus thermophilus is essential for induction of GTP hydrolysis on the ribosome by Martemyanov (2000)
{'key': '10.1016/j.cell.2014.11.049_bib32', 'first-page': '1879', 'article-title': 'Elongation factor G-induced structural change in helix 34 of 16S rRNA related to translocation on the ribosome', 'volume': '7', 'author': 'Matassova', 'year': '2001', 'journal-title': 'RNA'} / RNA / Elongation factor G-induced structural change in helix 34 of 16S rRNA related to translocation on the ribosome by Matassova (2001)
10.1107/S0021889807021206 / J. Appl. Cryst. / Phaser crystallographic software by McCoy (2007)
10.1021/bi026301y / Biochemistry / GTPase activation of elongation factors Tu and G on the ribosome by Mohr (2002)
10.1016/j.molcel.2007.01.022 / Mol. Cell / Identification of two distinct hybrid state intermediates on the ribosome by Munro (2007)
10.1038/nsmb.1925 / Nat. Struct. Mol. Biol. / Correlated conformational events in EF-G and the ribosome regulate translocation by Munro (2010)
10.1016/j.molcel.2007.01.014 / Mol. Cell / Kinetically competent intermediates in the translocation step of protein synthesis by Pan (2007)
10.1126/science.1235970 / Science / Control of ribosomal subunit rotation by elongation factor G by Pulk (2013)
10.1073/pnas.1320387110 / Proc. Natl. Acad. Sci. USA / Visualization of two transfer RNAs trapped in transit during elongation factor G-mediated translocation by Ramrath (2013)
10.1038/nature09547 / Nature / Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites by Ratje (2010)
10.1038/385037a0 / Nature / Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome by Rodnina (1997)
10.1073/pnas.96.17.9586 / Proc. Natl. Acad. Sci. USA / Thiostrepton inhibits the turnover but not the GTPase of elongation factor G on the ribosome by Rodnina (1999)
10.1073/pnas.1410873111 / Proc. Natl. Acad. Sci. USA / Following movement of domain IV of elongation factor G during ribosomal translocation by Salsi (2014)
10.1016/S1097-2765(03)00230-2 / Mol. Cell / An elongation factor G-induced ribosome rearrangement precedes tRNA-mRNA translocation by Savelsbergh (2003)
10.1038/sj.emboj.7600884 / EMBO J. / Control of phosphate release from elongation factor G by ribosomal protein L7/12 by Savelsbergh (2005)
10.1261/rna.601507 / RNA / Elongation factor G stabilizes the hybrid-state conformation of the 70S ribosome by Spiegel (2007)
10.1038/nsmb.1755 / Nat. Struct. Mol. Biol. / The structures of the anti-tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome by Stanley (2010)
10.1016/S0092-8674(00)80666-2 / Cell / Large-scale movement of elongation factor G and extensive conformational change of the ribosome during translocation by Stark (2000)
10.1038/emboj.2009.169 / EMBO J. / Conformational changes in switch I of EF-G drive its directional cycling on and off the ribosome by Ticu (2009)
10.1126/science.1235490 / Science / Elongation factor G bound to the ribosome in an intermediate state of translocation by Tourigny (2013)
10.1016/S0092-8674(03)00476-8 / Cell / Locking and unlocking of ribosomal motions by Valle (2003)
10.1146/annurev-biochem-113009-092313 / Annu. Rev. Biochem. / Structural basis of the translational elongation cycle by Voorhees (2013)
10.1038/nsmb.1577 / Nat. Struct. Mol. Biol. / Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome by Voorhees (2009)
10.1093/nar/gkr623 / Nucleic Acids Res. / Thiostrepton inhibits stable 70S ribosome binding and ribosome-dependent GTPase activation of elongation factor G and elongation factor 4 by Walter (2012)
10.1021/bi700657d / Biochemistry / Single-molecule structural dynamics of EF-G—ribosome interaction during translocation by Wang (2007)
10.1016/j.jmb.2004.01.013 / J. Mol. Biol. / Interactions of translational factor EF-G with the bacterial ribosome before and after mRNA translocation by Wilson (2004)
10.1126/science.1236086 / Science / Crystal structures of EF-G-ribosome complexes trapped in intermediate states of translocation by Zhou (2013)
10.1126/science.1255030 / Science / How the ribosome hands the A-site tRNA to the P site during EF-G-catalyzed translocation by Zhou (2014)
10.1016/0003-2697(89)90602-7 / Anal. Biochem. / Calculation of protein extinction coefficients from amino acid sequence data by Gill (1989)
10.1016/j.molcel.2008.01.009 / Mol. Cell / Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin by Harms (2008)
10.1093/nar/24.5.907 / Nucleic Acids Res. / In vivo deuteration of transfer RNAs: overexpression and large-scale purification of deuterated specific tRNAs by Jünemann (1996)
10.1126/science.1218538 / Science / How hibernation factors RMF, HPF, and YfiA turn off protein synthesis by Polikanov (2012)
10.1126/science.1179700 / Science / The crystal structure of the ribosome bound to EF-Tu and aminoacyl-tRNA by Schmeing (2009)

Dates

Type	When
Created	10 years, 7 months ago (Jan. 15, 2015, 11:46 a.m.)
Deposited	6 years ago (Aug. 19, 2019, 3:13 p.m.)
Indexed	3 months, 2 weeks ago (May 9, 2025, 9:33 p.m.)
Issued	10 years, 7 months ago (Jan. 1, 2015)
Published	10 years, 7 months ago (Jan. 1, 2015)
Published Print	10 years, 7 months ago (Jan. 1, 2015)

Funders 0

None

BibTeX

@article{Lin_2015, title={Conformational Changes of Elongation Factor G on the Ribosome during tRNA Translocation}, volume={160}, ISSN={0092-8674}, url={http://dx.doi.org/10.1016/j.cell.2014.11.049}, DOI={10.1016/j.cell.2014.11.049}, number={1–2}, journal={Cell}, publisher={Elsevier BV}, author={Lin, Jinzhong and Gagnon, Matthieu G. and Bulkley, David and Steitz, Thomas A.}, year={2015}, month=jan, pages={219–227} }

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      "article-title": "EF-G-dependent GTP hydrolysis induces translocation accompanied by large conformational changes in the 70S ribosome",
      "volume": "6",
      "author": "Agrawal",
      "year": "1999",
      "journal-title": "Nat. Struct. Biol."
    },
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      "doi-asserted-by": "crossref",
      "first-page": "966",
      "DOI": "10.1126/science.1175800",
      "article-title": "Formation of the first peptide bond: the structure of EF-P bound to the 70S ribosome",
      "volume": "325",
      "author": "Blaha",
      "year": "2009",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "12893",
      "DOI": "10.1073/pnas.0403884101",
      "article-title": "tRNA dynamics on the ribosome during translation",
      "volume": "101",
      "author": "Blanchard",
      "year": "2004",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
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      "first-page": "2934",
      "DOI": "10.1074/jbc.M407008200",
      "article-title": "Interaction of thiostrepton and elongation factor-G with the ribosomal protein L11-binding domain",
      "volume": "280",
      "author": "Bowen",
      "year": "2005",
      "journal-title": "J.\u00a0Biol. Chem."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "1262",
      "DOI": "10.1261/rna.61206",
      "article-title": "Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits",
      "volume": "12",
      "author": "Brandi",
      "year": "2006",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "3373",
      "DOI": "10.1016/j.febslet.2012.07.040",
      "article-title": "Structural and functional characterization of the bacterial translocation inhibitor GE82832",
      "volume": "586",
      "author": "Brandi",
      "year": "2012",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "20994",
      "DOI": "10.1073/pnas.1311423110",
      "article-title": "Structure of the ribosome with elongation factor G trapped in the pretranslocation state",
      "volume": "110",
      "author": "Brilot",
      "year": "2013",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "357",
      "DOI": "10.1016/j.celrep.2013.12.024",
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      "volume": "6",
      "author": "Bulkley",
      "year": "2014",
      "journal-title": "Cell Reports"
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    {
      "key": "10.1016/j.cell.2014.11.049_bib11",
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      "first-page": "367",
      "DOI": "10.1016/j.molcel.2011.03.024",
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      "volume": "42",
      "author": "Chen",
      "year": "2011",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "718",
      "DOI": "10.1038/nsmb.2567",
      "article-title": "Coordinated conformational and compositional dynamics drive ribosome translocation",
      "volume": "20",
      "author": "Chen",
      "year": "2013",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "1077",
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      "article-title": "Structure of EF-G-ribosome complex in a pretranslocation state",
      "volume": "20",
      "author": "Chen",
      "year": "2013",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "751",
      "DOI": "10.1016/j.molcel.2007.01.027",
      "article-title": "Structural basis for interaction of the ribosome with the switch regions of GTP-bound elongation factors",
      "volume": "25",
      "author": "Connell",
      "year": "2007",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "578",
      "DOI": "10.1016/j.molcel.2008.05.004",
      "article-title": "Spontaneous intersubunit rotation in single ribosomes",
      "volume": "30",
      "author": "Cornish",
      "year": "2008",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "321",
      "DOI": "10.1016/S1097-2765(03)00275-2",
      "article-title": "Ribosomal proteins S12 and S13 function as control elements for translocation of the mRNA:tRNA complex",
      "volume": "12",
      "author": "Cukras",
      "year": "2003",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "10327",
      "DOI": "10.1021/bi970610k",
      "article-title": "The conformational properties of elongation factor G and the mechanism of translocation",
      "volume": "36",
      "author": "Czworkowski",
      "year": "1997",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "3661",
      "DOI": "10.1002/j.1460-2075.1994.tb06675.x",
      "article-title": "The crystal structure of elongation factor G complexed with GDP, at 2.7 A resolution",
      "volume": "13",
      "author": "Czworkowski",
      "year": "1994",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib19",
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    {
      "key": "10.1016/j.cell.2014.11.049_bib20",
      "doi-asserted-by": "crossref",
      "first-page": "2126",
      "DOI": "10.1107/S0907444904019158",
      "article-title": "Coot: model-building tools for molecular graphics",
      "volume": "60",
      "author": "Emsley",
      "year": "2004",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "457",
      "DOI": "10.1038/nsmb.2011",
      "article-title": "mRNA translocation occurs during the second step of ribosomal intersubunit rotation",
      "volume": "18",
      "author": "Ermolenko",
      "year": "2011",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "348",
      "DOI": "10.1016/j.molcel.2008.03.012",
      "article-title": "Coupling of ribosomal L1 stalk and tRNA dynamics during translation elongation",
      "volume": "30",
      "author": "Fei",
      "year": "2008",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "318",
      "DOI": "10.1038/35018597",
      "article-title": "A ratchet-like inter-subunit reorganization of the ribosome during translocation",
      "volume": "406",
      "author": "Frank",
      "year": "2000",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "1370",
      "DOI": "10.1126/science.1217443",
      "article-title": "Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome",
      "volume": "335",
      "author": "Gagnon",
      "year": "2012",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "684",
      "DOI": "10.1126/science.1253525",
      "article-title": "Crystal structure of elongation factor 4 bound to a clockwise ratcheted ribosome",
      "volume": "345",
      "author": "Gagnon",
      "year": "2014",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "694",
      "DOI": "10.1126/science.1179709",
      "article-title": "The structure of the ribosome with elongation factor G trapped in the posttranslocational state",
      "volume": "326",
      "author": "Gao",
      "year": "2009",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "20391",
      "DOI": "10.1073/pnas.1218999109",
      "article-title": "Rotation of the head of the 30S ribosomal subunit during mRNA translocation",
      "volume": "109",
      "author": "Guo",
      "year": "2012",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "4492",
      "DOI": "10.1016/j.febslet.2005.07.016",
      "article-title": "Crystal structure of a mutant elongation factor G trapped with a GTP analogue",
      "volume": "579",
      "author": "Hansson",
      "year": "2005",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib29",
      "doi-asserted-by": "crossref",
      "first-page": "1073",
      "DOI": "10.1002/embj.201387465",
      "article-title": "GTP hydrolysis by EF-G synchronizes tRNA movement on small and large ribosomal subunits",
      "volume": "33",
      "author": "Holtkamp",
      "year": "2014",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "795",
      "DOI": "10.1107/S0021889893005588",
      "article-title": "Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants",
      "volume": "26",
      "author": "Kabsch",
      "year": "1993",
      "journal-title": "J.\u00a0Appl. Cryst."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "35820",
      "DOI": "10.1074/jbc.M002656200",
      "article-title": "Domain III of elongation factor G from Thermus thermophilus is essential for induction of GTP hydrolysis on the ribosome",
      "volume": "275",
      "author": "Martemyanov",
      "year": "2000",
      "journal-title": "J.\u00a0Biol. Chem."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib32",
      "first-page": "1879",
      "article-title": "Elongation factor G-induced structural change in helix 34 of 16S rRNA related to translocation on the ribosome",
      "volume": "7",
      "author": "Matassova",
      "year": "2001",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "658",
      "DOI": "10.1107/S0021889807021206",
      "article-title": "Phaser crystallographic software",
      "volume": "40",
      "author": "McCoy",
      "year": "2007",
      "journal-title": "J.\u00a0Appl. Cryst."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "12520",
      "DOI": "10.1021/bi026301y",
      "article-title": "GTPase activation of elongation factors Tu and G on the ribosome",
      "volume": "41",
      "author": "Mohr",
      "year": "2002",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "505",
      "DOI": "10.1016/j.molcel.2007.01.022",
      "article-title": "Identification of two distinct hybrid state intermediates on the ribosome",
      "volume": "25",
      "author": "Munro",
      "year": "2007",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib36",
      "doi-asserted-by": "crossref",
      "first-page": "1470",
      "DOI": "10.1038/nsmb.1925",
      "article-title": "Correlated conformational events in EF-G and the ribosome regulate translocation",
      "volume": "17",
      "author": "Munro",
      "year": "2010",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib37",
      "doi-asserted-by": "crossref",
      "first-page": "519",
      "DOI": "10.1016/j.molcel.2007.01.014",
      "article-title": "Kinetically competent intermediates in the translocation step of protein synthesis",
      "volume": "25",
      "author": "Pan",
      "year": "2007",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib38",
      "doi-asserted-by": "crossref",
      "first-page": "1235970",
      "DOI": "10.1126/science.1235970",
      "article-title": "Control of ribosomal subunit rotation by elongation factor G",
      "volume": "340",
      "author": "Pulk",
      "year": "2013",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib39",
      "doi-asserted-by": "crossref",
      "first-page": "20964",
      "DOI": "10.1073/pnas.1320387110",
      "article-title": "Visualization of two transfer RNAs trapped in transit during elongation factor G-mediated translocation",
      "volume": "110",
      "author": "Ramrath",
      "year": "2013",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib40",
      "doi-asserted-by": "crossref",
      "first-page": "713",
      "DOI": "10.1038/nature09547",
      "article-title": "Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites",
      "volume": "468",
      "author": "Ratje",
      "year": "2010",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib41",
      "doi-asserted-by": "crossref",
      "first-page": "37",
      "DOI": "10.1038/385037a0",
      "article-title": "Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome",
      "volume": "385",
      "author": "Rodnina",
      "year": "1997",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib42",
      "doi-asserted-by": "crossref",
      "first-page": "9586",
      "DOI": "10.1073/pnas.96.17.9586",
      "article-title": "Thiostrepton inhibits the turnover but not the GTPase of elongation factor G on the ribosome",
      "volume": "96",
      "author": "Rodnina",
      "year": "1999",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib43",
      "doi-asserted-by": "crossref",
      "first-page": "15060",
      "DOI": "10.1073/pnas.1410873111",
      "article-title": "Following movement of domain IV of elongation factor G during ribosomal translocation",
      "volume": "111",
      "author": "Salsi",
      "year": "2014",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib44",
      "doi-asserted-by": "crossref",
      "first-page": "1517",
      "DOI": "10.1016/S1097-2765(03)00230-2",
      "article-title": "An elongation factor G-induced ribosome rearrangement precedes tRNA-mRNA translocation",
      "volume": "11",
      "author": "Savelsbergh",
      "year": "2003",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib45",
      "doi-asserted-by": "crossref",
      "first-page": "4316",
      "DOI": "10.1038/sj.emboj.7600884",
      "article-title": "Control of phosphate release from elongation factor G by ribosomal protein L7/12",
      "volume": "24",
      "author": "Savelsbergh",
      "year": "2005",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib46",
      "doi-asserted-by": "crossref",
      "first-page": "1473",
      "DOI": "10.1261/rna.601507",
      "article-title": "Elongation factor G stabilizes the hybrid-state conformation of the 70S ribosome",
      "volume": "13",
      "author": "Spiegel",
      "year": "2007",
      "journal-title": "RNA"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib47",
      "doi-asserted-by": "crossref",
      "first-page": "289",
      "DOI": "10.1038/nsmb.1755",
      "article-title": "The structures of the anti-tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome",
      "volume": "17",
      "author": "Stanley",
      "year": "2010",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib48",
      "doi-asserted-by": "crossref",
      "first-page": "301",
      "DOI": "10.1016/S0092-8674(00)80666-2",
      "article-title": "Large-scale movement of elongation factor G and extensive conformational change of the ribosome during translocation",
      "volume": "100",
      "author": "Stark",
      "year": "2000",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib49",
      "doi-asserted-by": "crossref",
      "first-page": "2053",
      "DOI": "10.1038/emboj.2009.169",
      "article-title": "Conformational changes in switch I of EF-G drive its directional cycling on and off the ribosome",
      "volume": "28",
      "author": "Ticu",
      "year": "2009",
      "journal-title": "EMBO J."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib50",
      "doi-asserted-by": "crossref",
      "first-page": "1235490",
      "DOI": "10.1126/science.1235490",
      "article-title": "Elongation factor G bound to the ribosome in an intermediate state of translocation",
      "volume": "340",
      "author": "Tourigny",
      "year": "2013",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib51",
      "doi-asserted-by": "crossref",
      "first-page": "123",
      "DOI": "10.1016/S0092-8674(03)00476-8",
      "article-title": "Locking and unlocking of ribosomal motions",
      "volume": "114",
      "author": "Valle",
      "year": "2003",
      "journal-title": "Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib52",
      "doi-asserted-by": "crossref",
      "first-page": "203",
      "DOI": "10.1146/annurev-biochem-113009-092313",
      "article-title": "Structural basis of the translational elongation cycle",
      "volume": "82",
      "author": "Voorhees",
      "year": "2013",
      "journal-title": "Annu. Rev. Biochem."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib53",
      "doi-asserted-by": "crossref",
      "first-page": "528",
      "DOI": "10.1038/nsmb.1577",
      "article-title": "Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome",
      "volume": "16",
      "author": "Voorhees",
      "year": "2009",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib54",
      "doi-asserted-by": "crossref",
      "first-page": "360",
      "DOI": "10.1093/nar/gkr623",
      "article-title": "Thiostrepton inhibits stable 70S ribosome binding and ribosome-dependent GTPase activation of elongation factor G and elongation factor 4",
      "volume": "40",
      "author": "Walter",
      "year": "2012",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib55",
      "doi-asserted-by": "crossref",
      "first-page": "10767",
      "DOI": "10.1021/bi700657d",
      "article-title": "Single-molecule structural dynamics of EF-G\u2014ribosome interaction during translocation",
      "volume": "46",
      "author": "Wang",
      "year": "2007",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib56",
      "doi-asserted-by": "crossref",
      "first-page": "15",
      "DOI": "10.1016/j.jmb.2004.01.013",
      "article-title": "Interactions of translational factor EF-G with the bacterial ribosome before and after mRNA translocation",
      "volume": "337",
      "author": "Wilson",
      "year": "2004",
      "journal-title": "J.\u00a0Mol. Biol."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib57",
      "doi-asserted-by": "crossref",
      "first-page": "1236086",
      "DOI": "10.1126/science.1236086",
      "article-title": "Crystal structures of EF-G-ribosome complexes trapped in intermediate states of translocation",
      "volume": "340",
      "author": "Zhou",
      "year": "2013",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib58",
      "doi-asserted-by": "crossref",
      "first-page": "1188",
      "DOI": "10.1126/science.1255030",
      "article-title": "How the ribosome hands the A-site tRNA to the P site during EF-G-catalyzed translocation",
      "volume": "345",
      "author": "Zhou",
      "year": "2014",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib59",
      "doi-asserted-by": "crossref",
      "first-page": "319",
      "DOI": "10.1016/0003-2697(89)90602-7",
      "article-title": "Calculation of protein extinction coefficients from amino acid sequence data",
      "volume": "182",
      "author": "Gill",
      "year": "1989",
      "journal-title": "Anal. Biochem."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib60",
      "doi-asserted-by": "crossref",
      "first-page": "26",
      "DOI": "10.1016/j.molcel.2008.01.009",
      "article-title": "Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin",
      "volume": "30",
      "author": "Harms",
      "year": "2008",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib61",
      "doi-asserted-by": "crossref",
      "first-page": "907",
      "DOI": "10.1093/nar/24.5.907",
      "article-title": "In\u00a0vivo deuteration of transfer RNAs: overexpression and large-scale purification of deuterated specific tRNAs",
      "volume": "24",
      "author": "J\u00fcnemann",
      "year": "1996",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib62",
      "doi-asserted-by": "crossref",
      "first-page": "915",
      "DOI": "10.1126/science.1218538",
      "article-title": "How hibernation factors RMF, HPF, and YfiA turn off protein synthesis",
      "volume": "336",
      "author": "Polikanov",
      "year": "2012",
      "journal-title": "Science"
    },
    {
      "key": "10.1016/j.cell.2014.11.049_bib63",
      "doi-asserted-by": "crossref",
      "first-page": "688",
      "DOI": "10.1126/science.1179700",
      "article-title": "The crystal structure of the ribosome bound to EF-Tu and aminoacyl-tRNA",
      "volume": "326",
      "author": "Schmeing",
      "year": "2009",
      "journal-title": "Science"
    }
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