DOI: 10.1016/j.bpj.2015.10.043. Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms

Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms

10.1016/j.bpj.2015.10.043

Crossref journal-article

Elsevier BV

Biophysical Journal (78)

Bibliography

Fernandez, J.-J., Laugks, U., Schaffer, M., BÃ¤uerlein, F. J. B., Khoshouei, M., Baumeister, W., & Lucic, V. (2016). Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms. Biophysical Journal, 110(4), 850â859.

Authors 7

Jose-Jesus Fernandez (first)
Ulrike Laugks (additional)
Miroslava Schaffer (additional)
Felix J.B. Bäuerlein (additional)
Maryam Khoshouei (additional)
Wolfgang Baumeister (additional)
Vladan Lucic (additional)

References 30 Referenced 29

10.1146/annurev-matsci-070511-155004 / Annu. Rev. Mater. Res. / Electron microscopy of biological materials at the nanometer scale by Kourkoutis (2012)
10.1073/pnas.1201333109 / Proc. Natl. Acad. Sci. USA / Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography by Rigort (2012)
10.1038/nmeth1014 / Nat. Methods / Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy by Marko (2007)
10.1016/j.ultramic.2009.04.002 / Ultramicroscopy / Detective quantum efficiency of electron area detectors in electron microscopy by McMullan (2009)
10.1016/j.jsb.2011.09.002 / J. Struct. Biol. / Initial evaluation of a direct detection device detector for single particle cryo-electron microscopy by Milazzo (2011)
10.1073/pnas.1418377111 / Proc. Natl. Acad. Sci. USA / Volta potential phase plate for in-focus phase contrast transmission electron microscopy by Danev (2014)
10.1083/jcb.201304193 / J. Cell Biol. / Cryo-electron tomography: the challenge of doing structural biology in situ by Lučič (2013)
10.1016/j.micron.2012.05.003 / Micron / Computational methods for electron tomography by Fernandez (2012)
10.1006/jsbi.2001.4406 / J. Struct. Biol. / Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion by Frangakis (2001)
10.1016/j.jsb.2003.09.010 / J. Struct. Biol. / An improved algorithm for anisotropic nonlinear diffusion for denoising cryo-tomograms by Fernández (2003)
10.1016/j.jsb.2003.09.028 / J. Struct. Biol. / Applications of a bilateral denoising filter in biological electron microscopy by Jiang (2003)
10.1016/j.jsb.2006.10.030 / J. Struct. Biol. / Efficient automatic noise reduction of electron tomographic reconstructions based on iterative median filtering by van der Heide (2007)
10.1016/j.jsb.2011.08.012 / J. Struct. Biol. / Automated segmentation of electron tomograms for a quantitative description of actin filament networks by Rigort (2012)
10.1016/j.jsb.2014.02.004 / J. Struct. Biol. / A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions by Kováčik (2014)
10.1002/0471140864.ps1713s65 / Curr. Protoc. Protein Sci. / Cryo-electron tomography for structural characterization of macromolecular complexes by Cope (2011)
10.1016/j.jsb.2012.01.003 / J. Struct. Biol. / Removing high contrast artifacts via digital inpainting in cryo-electron tomography: an application of compressed sensing by Song (2012)
10.1016/j.jsb.2014.03.009 / J. Struct. Biol. / Local regularization of tilt projections reduces artifacts in electron tomography by Maiorca (2014)
10.1038/nprot.2006.356 / Nat. Protoc. / Culturing hippocampal neurons by Kaech (2006)
10.1016/j.jsb.2007.08.014 / J. Struct. Biol. / Multiscale imaging of neurons grown in culture: from light microscopy to cryo-electron tomography by Lucić (2007)
10.1016/j.jsb.2015.03.004 / J. Struct. Biol. / Electron cryotomography of vitrified cells with a Volta phase plate by Fukuda (2015)
10.1016/0006-8993(88)91383-2 / Brain Res. / A rapid Percoll gradient procedure for isolation of synaptosomes directly from an S1 fraction: homogeneity and morphology of subcellular fractions by Dunkley (1988)
10.1111/j.1471-4159.2006.04422.x / J. Neurochem. / CB1 receptors diminish both Ca2+ influx and glutamate release through two different mechanisms active in distinct populations of cerebrocortical nerve terminals by Godino (2007)
10.1083/jcb.200908082 / J. Cell Biol. / Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography by Fernández-Busnadiego (2010)
10.1111/j.1365-2818.2007.01775.x / J. Microsc. / A technique for improved focused ion beam milling of cryo-prepared life science specimens by Hayles (2007)
10.1016/j.jsb.2005.07.007 / J. Struct. Biol. / Automated electron microscope tomography using robust prediction of specimen movements by Mastronarde (2005)
10.1006/jsbi.1997.3919 / J. Struct. Biol. / Dual-axis tomography: an approach with alignment methods that preserve resolution by Mastronarde (1997)
10.1093/bioinformatics/btq692 / Bioinformatics / Fast tomographic reconstruction on multicore computers by Agulleiro (2011)
10.1083/jcb.201206063 / J. Cell Biol. / Cryo-electron tomography reveals a critical role of RIM1α in synaptic vesicle tethering by Fernández-Busnadiego (2013)
10.1016/j.jsb.2011.05.010 / J. Struct. Biol. / A differential structure approach to membrane segmentation in electron tomography by Martinez-Sanchez (2011)
10.1038/nature12104 / Nature / Structures of the human and Drosophila 80S ribosome by Anger (2013)

Dates

Type	When
Created	9 years, 8 months ago (Dec. 29, 2015, 11 p.m.)
Deposited	6 years, 11 months ago (Sept. 16, 2018, 9:25 a.m.)
Indexed	5 months, 2 weeks ago (March 19, 2025, 10:15 a.m.)
Issued	9 years, 7 months ago (Feb. 1, 2016)
Published	9 years, 7 months ago (Feb. 1, 2016)
Published Print	9 years, 7 months ago (Feb. 1, 2016)

Funders 0

None

BibTeX

@article{Fernandez_2016, title={Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms}, volume={110}, ISSN={0006-3495}, url={http://dx.doi.org/10.1016/j.bpj.2015.10.043}, DOI={10.1016/j.bpj.2015.10.043}, number={4}, journal={Biophysical Journal}, publisher={Elsevier BV}, author={Fernandez, Jose-Jesus and Laugks, Ulrike and Schaffer, Miroslava and Bäuerlein, Felix J.B. and Khoshouei, Maryam and Baumeister, Wolfgang and Lucic, Vladan}, year={2016}, month=feb, pages={850–859} }

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  "URL": "http://dx.doi.org/10.1016/j.bpj.2015.10.043",
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      "value": "Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms",
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