DOI: 10.1016/j.bbrc.2003.11.177. Guideline to reference gene selection for quantitative real-time PCR

Guideline to reference gene selection for quantitative real-time PCR

10.1016/j.bbrc.2003.11.177

Crossref journal-article

Elsevier BV

Biochemical and Biophysical Research Communications (78)

Bibliography

RadoniÄ, A., Thulke, S., Mackay, I. M., Landt, O., Siegert, W., & Nitsche, A. (2004). Guideline to reference gene selection for quantitative real-time PCR. Biochemical and Biophysical Research Communications, 313(4), 856â862.

Authors 6

Aleksandar Radonić (first)
Stefanie Thulke (additional)
Ian M Mackay (additional)
Olfert Landt (additional)
Wolfgang Siegert (additional)
Andreas Nitsche (additional)

References 24 Referenced 1,326

10.1093/nar/30.6.1292 / Nucleic Acids Res. / Real-time PCR in virology by Mackay (2002)
10.1016/S0301-472X(02)00806-8 / Exp. Hematol. / Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream by Ginzinger (2002)
10.1677/jme.0.0290023 / J. Mol. Endocrinol. / Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems by Bustin (2002)
10.2144/00292rv02 / Biotechniques / Control selection for RNA quantitation by Suzuki (2000)
10.1006/mcpr.2001.0376 / Mol. Cell Probes / Beta-actin—an unsuitable internal control for RT-PCR by Selvey (2001)
10.1136/thorax.57.9.765 / Thorax / Beta-actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels by Glare (2002)
10.1006/bbrc.1999.0815 / Biochem. Biophys. Res. Commun. / Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia by Zhong (1999)
10.1023/A:1016166127465 / Mol. Cell. Biochem. / Differential expression of GAPDH and beta3-actin in growing collateral arteries by Deindl (2002)
10.1006/abio.2001.5369 / Anal. Biochem. / Identification and validation of endogenous reference genes for expression profiling of T helper cell differentiation by quantitative real-time RT-PCR by Hamalainen (2001)
10.1055/s-2001-17911 / Horm. Metab. Res. / Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytes by Gorzelniak (2001)
10.1016/S0301-472X(02)00787-7 / Exp. Hematol. / Quantitative assessment of gene expression in highly purified hematopoietic cells using real-time reverse transcriptase polymerase chain reaction by Raaijmakers (2002)
10.1186/gb-2002-3-7-research0034 / Genome Biol. / Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes by Vandesompele (2002)
10.1006/abio.2001.5570 / Anal. Biochem. / Housekeeping gene variability in normal and carcinomatous colorectal and liver tissues: applications in pharmacogenomic gene expression studies by Blanquicett (2002)
10.1016/S0168-1656(99)00163-7 / J. Biotechnol. / Housekeeping genes as internal standards: use and limits by Thellin (1999)
10.1016/S0003-2697(02)00311-1 / Anal. Biochem. / Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies by Tricarico (2002)
10.1093/clinchem/45.2.297 / Clin. Chem. / Highly sensitive and specific fluorescence reverse transcription-PCR assay for the pseudogene-free detection of beta-actin transcripts as quantitative reference by Kreuzer (1999)
10.1128/AEM.60.1.149-152.1994 / Appl. Environ. Microbiol. / Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR by van Kuppeveld (1994)
10.1124/jpet.301.2.738 / J. Pharmacol. Exp. Ther. / Anti-inflammatory effects of a cyclosporine receptor-binding compound, D-43787 by Pahl (2002)
10.1126/science.296.5567.557 / Science / Tech. Sight. A technique whose time has come by Walker (2002)
10.1093/nar/30.9.e36 / Nucleic Acids Res. / Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR by Pfaffl (2002)
10.1093/nar/29.9.e45 / Nucleic Acids Res. / A new mathematical model for relative quantification in real-time RT-PCR by Pfaffl (2001)
10.1016/S0169-328X(02)00417-5 / Brain Res. Mol. Brain Res. / The quantification of gene expression in an animal model of brain ischaemia using TaqMan real-time RT-PCR by Bond (2002)
10.1016/S0165-022X(00)00129-9 / J. Biochem. Biophys. Methods / Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR by Schmittgen (2000)
10.1006/abio.2000.4889 / Anal. Biochem. / Unsuitability of using ribosomal RNA as loading control for Northern blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors by Solanas (2001)

Dates

Type	When
Created	21 years, 8 months ago (Dec. 19, 2003, 5:57 a.m.)
Deposited	5 years, 5 months ago (March 28, 2020, 2:16 a.m.)
Indexed	50 minutes ago (Sept. 4, 2025, 8:05 a.m.)
Issued	21 years, 8 months ago (Jan. 1, 2004)
Published	21 years, 8 months ago (Jan. 1, 2004)
Published Print	21 years, 8 months ago (Jan. 1, 2004)

Funders 0

None

BibTeX

@article{Radoni__2004, title={Guideline to reference gene selection for quantitative real-time PCR}, volume={313}, ISSN={0006-291X}, url={http://dx.doi.org/10.1016/j.bbrc.2003.11.177}, DOI={10.1016/j.bbrc.2003.11.177}, number={4}, journal={Biochemical and Biophysical Research Communications}, publisher={Elsevier BV}, author={Radonić, Aleksandar and Thulke, Stefanie and Mackay, Ian M and Landt, Olfert and Siegert, Wolfgang and Nitsche, Andreas}, year={2004}, month=jan, pages={856–862} }

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