DOI: 10.1016/bs.mcb.2020.12.009. Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells

Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells

10.1016/bs.mcb.2020.12.009

Crossref book-chapter

Elsevier

Methods in Cell Biology (78)

Bibliography

Klein, S., Wachsmuth-Melm, M., Winter, S. L., Kolovou, A., & Chlanda, P. (2021). Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells. Correlative Light and Electron Microscopy IV, 273â302.

Authors 5

Steffen Klein (first)
Moritz Wachsmuth-Melm (additional)
Sophie L. Winter (additional)
Androniki Kolovou (additional)
Petr Chlanda (additional)

References 28 Referenced 26

10.1017/S1431927603032008 / Microscopy and Microanalysis / Cryo-electron microscopy of vitreous sections of biological specimens by Al-Amoudi (2003)
10.1016/j.bpj.2015.10.053 / Biophysical Journal / Site-specific cryo-focused ion beam sample preparation guided by 3D correlative microscopy by Arnold (2016)
10.1007/978-1-62703-776-1_10 / Methods in molecular biology / Cryo-electron microscopy of vitreous sections by Chlanda (2014)
10.1038/nmeth.2075 / Nature Methods / Icy: An open bioimage informatics platform for extended reproducible research by de Chaumont (2012)
10.1017/S0033583500004297 / Quarterly Reviews of Biophysics / Cryo-electron microscopy of vitrified specimens by Dubochet (1988)
10.7554/eLife.45919 / eLife / PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy by Gorelick (2019)
10.1016/j.jsb.2016.06.007 / Journal of Structural Biology / Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging by Hagen (2017)
{'year': '2020', 'series-title': 'Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies', 'author': 'Klein', 'key': '10.1016/bs.mcb.2020.12.009_bb0045'} / Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies by Klein (2020)
10.1016/bs.mcb.2017.03.011 / Methods in Cell Biology / A new method for cryo-sectioning cell monolayers using a correlative workflow by Kolovou (2017)
10.1006/jsbi.1996.0013 / Journal of Structural Biology / Computer visualization of three-dimensional image data using IMOD by Kremer (1996)
10.1083/jcb.201004104 / The Journal of Cell Biology / Metadata matters: Access to image data in the real world by Linkert (2010)
10.1083/jcb.201304193 / The Journal of Cell Biology / Cryo-electron tomography: The challenge of doing structural biology in situ by Lučič (2013)
10.1126/science.aad8857 / Science / Visualizing the molecular sociology at the HeLa cell nuclear periphery by Mahamid (2016)
10.1038/nmeth1014 / Nature Methods / Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy by Marko (2007)
10.1016/j.jsb.2005.07.007 / Journal of Structural Biology / Automated electron microscope tomography using robust prediction of specimen movements by Mastronarde (2005)
{'author': 'Microscopy Nyquist Rate and PSF Calculator.', 'key': '10.1016/bs.mcb.2020.12.009_bb0085'} by Microscopy Nyquist Rate and PSF Calculator.
10.1109/T-AIEE.1928.5055024 / Transactions of the American Institute of Electrical Engineers / Certain topics in telegraph transmission theory by Nyquist (1928)
10.1038/nmeth.4170 / Nature Methods / eC-CLEM: Flexible multidimensional registration software for correlative microscopies by Paul-Gilloteaux (2017)
10.1093/bioinformatics/btp184 / Bioinformatics / Globally optimal stitching of tiled 3D microscopic image acquisitions by Preibisch (2009)
10.1073/pnas.1201333109 / Proceedings of the National Academy of Sciences of the United States of America / Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography by Rigort (2012)
10.1038/s41467-018-08178-7 / Nature Communications / Correlative cryo-electron microscopy reveals the structure of TNTs in neuronal cells by Sartori-Rupp (2019)
10.1016/j.jsb.2016.07.010 / Journal of Structural Biology / Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins by Schaffer (2017)
10.1016/j.jsb.2016.06.020 / Journal of Structural Biology / New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography by Schorb (2017)
10.1002/j.1538-7305.1948.tb00917.x / Bell System Technical Journal / A mathematical theory of communication by Shannon (1948)
10.1038/s41592-019-0630-5 / Nature Methods / Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies by Toro-Nahuelpan (2020)
{'year': '2020', 'series-title': 'cryoCLEM FIJI plugin', 'author': 'Wachsmuth-Melm', 'key': '10.1016/bs.mcb.2020.12.009_bb0135'} / cryoCLEM FIJI plugin by Wachsmuth-Melm (2020)
10.1038/s41596-020-0320-x / Nature Protocols / Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography by Wagner (2020)
10.1016/bs.mie.2016.04.014 / Methods in Enzymology / Cryo-electron tomography and subtomogram averaging by Wan (2016)

Dates

Type	When
Created	4 years, 6 months ago (Feb. 17, 2021, 9:35 p.m.)
Deposited	2 years, 7 months ago (Jan. 3, 2023, 9:32 a.m.)
Indexed	1 month ago (July 16, 2025, 8:16 a.m.)
Issued	4 years, 7 months ago (Jan. 1, 2021)
Published	4 years, 7 months ago (Jan. 1, 2021)
Published Print	4 years, 7 months ago (Jan. 1, 2021)

Funders 0

None

BibTeX

@inbook{Klein_2021, title={Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells}, ISBN={9780128220580}, ISSN={0091-679X}, url={http://dx.doi.org/10.1016/bs.mcb.2020.12.009}, DOI={10.1016/bs.mcb.2020.12.009}, booktitle={Correlative Light and Electron Microscopy IV}, publisher={Elsevier}, author={Klein, Steffen and Wachsmuth-Melm, Moritz and Winter, Sophie L. and Kolovou, Androniki and Chlanda, Petr}, year={2021}, pages={273–302} }

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