DOI: 10.1016/s0969-2126(96)00143-8. A pancreatic lipase with a phospholipase A1 activity: crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig

A pancreatic lipase with a phospholipase A1 activity: crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig

10.1016/s0969-2126(96)00143-8

Crossref journal-article

Elsevier BV

Structure (78)

Bibliography

Withers-Martinez, C., CarriÃ¨re, F., Verger, R., Bourgeois, D., & Cambillau, C. (1996). A pancreatic lipase with a phospholipase A1 activity: crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig. Structure, 4(11), 1363â1374.

Authors 5

Chrislaine Withers-Martinez (first)
Frédéric Carrière (additional)
Robert Verger (additional)
Dominique Bourgeois (additional)
Christian Cambillau (additional)

References 45 Referenced 94

10.1016/0076-6879(91)97147-Q / Methods Enzymol / Cloning expression and purification of porcine pancreatic phospholipase A2 and mutants by Verheij (1991)
10.1016/0006-3002(58)90097-0 / Biochim. Biophys. Acta / Action de la lipase pancréatique sur les esters en émulsion by Sarda (1958)
10.1016/0005-2795(71)90347-3 / Biochim. Biophys. Acta / Isolation and partial identification of a pancreatic colipase by Maylié (1971)
{'key': '10.1016/S0969-2126(96)00143-8_BIB4', 'first-page': '509', 'article-title': 'Pancreatic juice co-lipase physiological importance', 'volume': '242', 'author': 'Borgström', 'year': '1971', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta / Pancreatic juice co-lipase physiological importance by Borgström (1971)
10.1038/343771a0 / Nature / Structure of human pancreatic lipase by Winkler (1990)
10.1038/359159a0 / Nature / Structure of the pancreatic lipase-procolipase complex by van Tilbeurgh (1992)
10.1038/362814a0 / Nature / Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography by van Tilbeurgh (1993)
10.1006/jmbi.1994.1331 / J. Mol. Biol / Horse pancreatic lipase: the crystal structure refined at 2.3 å resolution by Bourne (1994)
10.1021/bi00009a003 / Biochemistry / The 2.46 å resolution structure of the pancreatic lipase–colipase complex inhibited by a C11 alkyl phosphonate by Egloff (1995)
10.1016/S0021-9258(18)42032-7 / J. Biol. Chem / Two novel human pancreatic lipase related proteins HPLRP1 and HPLRP2 differences in colipase dependence and in lipase activity by Giller (1992)
10.1021/bi00069a003 / Biochemistry / A structural domain (the lid) found in pancreatic lipases is absent in the guinea pig (phospho)lipase by Hjorth (1993)
10.1016/0005-2760(81)90173-9 / Biochim. Biophys. Acta / Purification of two lipases with high phospholipase A1 activity from guinea pig pancreas by Fauvel (1981)
10.1021/bi00176a002 / Biochemistry / Evidence for a pancreatic lipase subfamily with new kinetic properties by Thirstrup (1994)
10.1016/0005-2760(96)00068-9 / Biochim. Biophys. Acta / Pancreatic lipase-related protein 2 but the classical pancreatic lipase hydrolyzes galactolipids by Andersson (1996)
10.1093/protein/7.4.563 / Prot. Eng / Structure-function relationships in naturally occurring mutants of pancreatic lipase by Carrière (1994)
10.1016/0005-2760(81)90092-8 / Biochim. Biophys. Acta / Evidence for the lack of classical secretory phospholipase A2 in guinea pig pancreas by Fauvel (1981)
10.1016/0014-5793(94)80117-7 / FEBS Lett / Cloning of the classical guinea pig pancreatic lipase and comparison with the lipase related protein 2 by Carrière (1994)
10.1073/pnas.86.24.9647 / Proc. Natl. Acad. Sci. USA / Organization of the human lipoprotein lipase gene and evolution of the lipase gene family by Kirchgessner (1989)
10.1016/0014-5793(93)80080-E / FEBS Lett / Sequence similarity of a hornet (D. maculata) venom allergen phospholipase A1 with mammalian lipases by Soldatova (1993)
10.1159/000236728 / Int. Arch. Allergy Immunol / Allergens in hymenoptera venom. XXVI: The complete amino acid sequences of two vespid venom phospholipases by Hoffman (1994)
10.1016/0091-6749(85)90040-5 / J. Allergy Clin. Immunol / Antigenic cross-reactivity of venom proteins from hornets wasps and yellowjackets by King (1985)
10.1016/0005-2760(88)90309-8 / Biochim. Biophys. Acta / Purification and characterization of a lethal protein with phospholipase A1 activity from the hornet (Vespa basalis) venom by Ho (1988)
10.1016/0022-2836(89)90380-X / J. Mol. Biol / Crystallization and preliminary X-ray study of horse pancreatic lipase by Lombardo (1989)
10.1107/S0108767393007597 / Acta Cryst. A / AMoRe: an automated package for molecular replacement by Navaza (1994)
{'key': '10.1016/S0969-2126(96)00143-8_BIB25', 'series-title': 'X-PLOR Version 3.1 manual', 'author': 'Brünger', 'year': '1992'} / X-PLOR Version 3.1 manual by Brünger (1992)
10.1107/S0021889892009944 / J. Appl. Cryst / PROCHECK: a program to check the stereochemical quality of protein structures by Laskowski (1993)
10.1038/334528a0 / Nature / The molecular evolution of genes and proteins: a tale of two serines by Brenner (1988)
10.1093/protein/5.3.197 / Protein Eng / The α/β hydrolase fold by Ollis (1992)
10.1016/0022-2836(89)90583-4 / J. Mol. Biol / Conformation of β-hairpins in protein structures by Sibanda (1989)
10.1021/bi00167a011 / Biochemistry / Cutinase a lipolytic enzyme with a preformed oxyanion hole by Martinez (1994)
10.1038/343767a0 / Nature / A serine protease triad forms the catalytic centre of a triacylglycerol lipase by Brady (1990)
10.1038/351491a0 / Nature / A model for interfacial activation in lipases from the structure of a fungal lipase inhibitor complex by Brzozowski (1991)
10.1021/ja00087a002 / J. Am. Chem. Soc / A structural basis for the chiral preferences of lipases by Cygler (1994)
10.1021/bi00051a035 / Biochemistry / Crystallographic and molecular-modeling studies of lipase B from Candida antartica reveal a stereospecificity pocket for secondary alcohols by Uppenberg (1995)
{'key': '10.1016/S0969-2126(96)00143-8_BIB35', 'series-title': 'PCR Technology', 'first-page': '31', 'article-title': 'Principles and applications for DNA amplification', 'author': 'Higuchi', 'year': '1992'} / PCR Technology / Principles and applications for DNA amplification by Higuchi (1992)
10.1016/0014-5793(93)81044-Z / FEBS Lett / One-step purification and characterization of human pancreatic lipase expressed in insect cells by Thirstrup (1993)
10.1111/j.1432-1033.1990.tb15454.x / Eur. J. Biochem / Current approaches to macromolecular crystallization by McPherson (1990)
10.1016/0022-0248(91)90896-D / Journal of Crystal Growth / Application of the streak seeding technique in protein crystallization by Stura (1991)
10.1107/S0021889895002706 / J. Appl. Cryst / Increasing the size of microcrystals by fine sampling of pH limits by McPherson (1995)
10.1016/0022-2836(68)90205-2 / J. Mol. Biol / Solvent content of protein crystals by Matthews (1968)
10.1063/1.1143716 / Rev. Sci. Instrum / Bent crystal, bent multilayer optics on a multipole wiggler line for an X-ray diffractometer with an imaging plate detector (biological crystallography) by Fourme (1992)
{'key': '10.1016/S0969-2126(96)00143-8_BIB42', 'series-title': 'Proceedings of the CCP4 Study Weekend: Data Collection and Processing', 'first-page': '56', 'article-title': 'Oscillation Data Reduction Program', 'author': 'Otwinowski', 'year': '1993'} / Proceedings of the CCP4 Study Weekend: Data Collection and Processing / Oscillation Data Reduction Program by Otwinowski (1993)
10.1107/S0108767391001071 / Acta Cryst. A / Accurate bond and angle parameters for X-ray protein structure refinement by Engh (1991)
{'key': '10.1016/S0969-2126(96)00143-8_BIB44', 'series-title': 'GRASP manual', 'author': 'Nicholls', 'year': '1993'} / GRASP manual by Nicholls (1993)
10.1016/0005-2744(68)90248-9 / Biochim. Biophys. Acta / Purification and properties of phospholipase A from porcine pancreas by De Haas (1968)

Dates

Type	When
Created	20 years, 6 months ago (Feb. 9, 2005, 8:11 p.m.)
Deposited	4 years, 2 months ago (July 4, 2021, 7:10 p.m.)
Indexed	1 month, 1 week ago (July 28, 2025, 5:38 p.m.)
Issued	28 years, 10 months ago (Nov. 1, 1996)
Published	28 years, 10 months ago (Nov. 1, 1996)
Published Print	28 years, 10 months ago (Nov. 1, 1996)

Funders 0

None

BibTeX

@article{Withers_Martinez_1996, title={A pancreatic lipase with a phospholipase A1 activity: crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig}, volume={4}, ISSN={0969-2126}, url={http://dx.doi.org/10.1016/s0969-2126(96)00143-8}, DOI={10.1016/s0969-2126(96)00143-8}, number={11}, journal={Structure}, publisher={Elsevier BV}, author={Withers-Martinez, Chrislaine and Carrière, Frédéric and Verger, Robert and Bourgeois, Dominique and Cambillau, Christian}, year={1996}, month=nov, pages={1363–1374} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        28
      ]
    ],
    "date-time": "2025-07-28T21:38:08Z",
    "timestamp": 1753738688210
  },
  "reference-count": 45,
  "publisher": "Elsevier BV",
  "issue": "11",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1996,
            11,
            1
          ]
        ],
        "date-time": "1996-11-01T00:00:00Z",
        "timestamp": 846806400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    },
    {
      "start": {
        "date-parts": [
          [
            2013,
            7,
            17
          ]
        ],
        "date-time": "2013-07-17T00:00:00Z",
        "timestamp": 1374019200000
      },
      "content-version": "vor",
      "delay-in-days": 6102,
      "URL": "https://www.elsevier.com/open-access/userlicense/1.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1996,
        11
      ]
    ]
  },
  "DOI": "10.1016/s0969-2126(96)00143-8",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        2,
        10
      ]
    ],
    "date-time": "2005-02-10T01:11:04Z",
    "timestamp": 1107997864000
  },
  "page": "1363-1374",
  "source": "Crossref",
  "is-referenced-by-count": 94,
  "title": "A pancreatic lipase with a phospholipase A1 activity: crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig",
  "prefix": "10.1016",
  "volume": "4",
  "author": [
    {
      "given": "Chrislaine",
      "family": "Withers-Martinez",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Fr\u00e9d\u00e9ric",
      "family": "Carri\u00e8re",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Robert",
      "family": "Verger",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Dominique",
      "family": "Bourgeois",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Christian",
      "family": "Cambillau",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB1",
      "doi-asserted-by": "crossref",
      "first-page": "214",
      "DOI": "10.1016/0076-6879(91)97147-Q",
      "article-title": "Cloning expression and purification of porcine pancreatic phospholipase A2 and mutants",
      "volume": "197",
      "author": "Verheij",
      "year": "1991",
      "journal-title": "Methods Enzymol"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB2",
      "doi-asserted-by": "crossref",
      "first-page": "513",
      "DOI": "10.1016/0006-3002(58)90097-0",
      "article-title": "Action de la lipase pancr\u00e9atique sur les esters en \u00e9mulsion",
      "volume": "30",
      "author": "Sarda",
      "year": "1958",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB3",
      "doi-asserted-by": "crossref",
      "first-page": "286",
      "DOI": "10.1016/0005-2795(71)90347-3",
      "article-title": "Isolation and partial identification of a pancreatic colipase",
      "volume": "229",
      "author": "Mayli\u00e9",
      "year": "1971",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB4",
      "first-page": "509",
      "article-title": "Pancreatic juice co-lipase physiological importance",
      "volume": "242",
      "author": "Borgstr\u00f6m",
      "year": "1971",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB5",
      "doi-asserted-by": "crossref",
      "first-page": "771",
      "DOI": "10.1038/343771a0",
      "article-title": "Structure of human pancreatic lipase",
      "volume": "343",
      "author": "Winkler",
      "year": "1990",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB6",
      "doi-asserted-by": "crossref",
      "first-page": "159",
      "DOI": "10.1038/359159a0",
      "article-title": "Structure of the pancreatic lipase-procolipase complex",
      "volume": "359",
      "author": "van Tilbeurgh",
      "year": "1992",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB7",
      "doi-asserted-by": "crossref",
      "first-page": "814",
      "DOI": "10.1038/362814a0",
      "article-title": "Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography",
      "volume": "362",
      "author": "van Tilbeurgh",
      "year": "1993",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB8",
      "doi-asserted-by": "crossref",
      "first-page": "709",
      "DOI": "10.1006/jmbi.1994.1331",
      "article-title": "Horse pancreatic lipase: the crystal structure refined at 2.3 \u00e5 resolution",
      "volume": "238",
      "author": "Bourne",
      "year": "1994",
      "journal-title": "J. Mol. Biol"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB9",
      "doi-asserted-by": "crossref",
      "first-page": "2751",
      "DOI": "10.1021/bi00009a003",
      "article-title": "The 2.46 \u00e5 resolution structure of the pancreatic lipase\u2013colipase complex inhibited by a C11 alkyl phosphonate",
      "volume": "34",
      "author": "Egloff",
      "year": "1995",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB10",
      "doi-asserted-by": "crossref",
      "first-page": "16509",
      "DOI": "10.1016/S0021-9258(18)42032-7",
      "article-title": "Two novel human pancreatic lipase related proteins HPLRP1 and HPLRP2 differences in colipase dependence and in lipase activity",
      "volume": "267",
      "author": "Giller",
      "year": "1992",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB11",
      "doi-asserted-by": "crossref",
      "first-page": "4202",
      "DOI": "10.1021/bi00069a003",
      "article-title": "A structural domain (the lid) found in pancreatic lipases is absent in the guinea pig (phospho)lipase",
      "volume": "32",
      "author": "Hjorth",
      "year": "1993",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB12",
      "doi-asserted-by": "crossref",
      "first-page": "446",
      "DOI": "10.1016/0005-2760(81)90173-9",
      "article-title": "Purification of two lipases with high phospholipase A1 activity from guinea pig pancreas",
      "volume": "663",
      "author": "Fauvel",
      "year": "1981",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB13",
      "doi-asserted-by": "crossref",
      "first-page": "2748",
      "DOI": "10.1021/bi00176a002",
      "article-title": "Evidence for a pancreatic lipase subfamily with new kinetic properties",
      "volume": "33",
      "author": "Thirstrup",
      "year": "1994",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB14",
      "doi-asserted-by": "crossref",
      "first-page": "236",
      "DOI": "10.1016/0005-2760(96)00068-9",
      "article-title": "Pancreatic lipase-related protein 2 but the classical pancreatic lipase hydrolyzes galactolipids",
      "volume": "1302",
      "author": "Andersson",
      "year": "1996",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB15",
      "doi-asserted-by": "crossref",
      "first-page": "563",
      "DOI": "10.1093/protein/7.4.563",
      "article-title": "Structure-function relationships in naturally occurring mutants of pancreatic lipase",
      "volume": "7",
      "author": "Carri\u00e8re",
      "year": "1994",
      "journal-title": "Prot. Eng"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB16",
      "doi-asserted-by": "crossref",
      "first-page": "72",
      "DOI": "10.1016/0005-2760(81)90092-8",
      "article-title": "Evidence for the lack of classical secretory phospholipase A2 in guinea pig pancreas",
      "volume": "666",
      "author": "Fauvel",
      "year": "1981",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB17",
      "doi-asserted-by": "crossref",
      "first-page": "63",
      "DOI": "10.1016/0014-5793(94)80117-7",
      "article-title": "Cloning of the classical guinea pig pancreatic lipase and comparison with the lipase related protein 2",
      "volume": "338",
      "author": "Carri\u00e8re",
      "year": "1994",
      "journal-title": "FEBS Lett"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB18",
      "doi-asserted-by": "crossref",
      "first-page": "9647",
      "DOI": "10.1073/pnas.86.24.9647",
      "article-title": "Organization of the human lipoprotein lipase gene and evolution of the lipase gene family",
      "volume": "86",
      "author": "Kirchgessner",
      "year": "1989",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB19",
      "doi-asserted-by": "crossref",
      "first-page": "145",
      "DOI": "10.1016/0014-5793(93)80080-E",
      "article-title": "Sequence similarity of a hornet (D. maculata) venom allergen phospholipase A1 with mammalian lipases",
      "volume": "320",
      "author": "Soldatova",
      "year": "1993",
      "journal-title": "FEBS Lett"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB20",
      "doi-asserted-by": "crossref",
      "first-page": "184",
      "DOI": "10.1159/000236728",
      "article-title": "Allergens in hymenoptera venom. XXVI: The complete amino acid sequences of two vespid venom phospholipases",
      "volume": "104",
      "author": "Hoffman",
      "year": "1994",
      "journal-title": "Int. Arch. Allergy Immunol"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB21",
      "doi-asserted-by": "crossref",
      "first-page": "621",
      "DOI": "10.1016/0091-6749(85)90040-5",
      "article-title": "Antigenic cross-reactivity of venom proteins from hornets wasps and yellowjackets",
      "volume": "75",
      "author": "King",
      "year": "1985",
      "journal-title": "J. Allergy Clin. Immunol"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "414",
      "DOI": "10.1016/0005-2760(88)90309-8",
      "article-title": "Purification and characterization of a lethal protein with phospholipase A1 activity from the hornet (Vespa basalis) venom",
      "volume": "963",
      "author": "Ho",
      "year": "1988",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB23",
      "doi-asserted-by": "crossref",
      "first-page": "259",
      "DOI": "10.1016/0022-2836(89)90380-X",
      "article-title": "Crystallization and preliminary X-ray study of horse pancreatic lipase",
      "volume": "205",
      "author": "Lombardo",
      "year": "1989",
      "journal-title": "J. Mol. Biol"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB24",
      "doi-asserted-by": "crossref",
      "first-page": "157",
      "DOI": "10.1107/S0108767393007597",
      "article-title": "AMoRe: an automated package for molecular replacement",
      "volume": "50",
      "author": "Navaza",
      "year": "1994",
      "journal-title": "Acta Cryst. A"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB25",
      "series-title": "X-PLOR Version 3.1 manual",
      "author": "Br\u00fcnger",
      "year": "1992"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "283",
      "DOI": "10.1107/S0021889892009944",
      "article-title": "PROCHECK: a program to check the stereochemical quality of protein structures",
      "volume": "26",
      "author": "Laskowski",
      "year": "1993",
      "journal-title": "J. Appl. Cryst"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "528",
      "DOI": "10.1038/334528a0",
      "article-title": "The molecular evolution of genes and proteins: a tale of two serines",
      "volume": "334",
      "author": "Brenner",
      "year": "1988",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB28",
      "doi-asserted-by": "crossref",
      "first-page": "197",
      "DOI": "10.1093/protein/5.3.197",
      "article-title": "The \u03b1/\u03b2 hydrolase fold",
      "volume": "5",
      "author": "Ollis",
      "year": "1992",
      "journal-title": "Protein Eng"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "759",
      "DOI": "10.1016/0022-2836(89)90583-4",
      "article-title": "Conformation of \u03b2-hairpins in protein structures",
      "volume": "206",
      "author": "Sibanda",
      "year": "1989",
      "journal-title": "J. Mol. Biol"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB30",
      "doi-asserted-by": "crossref",
      "first-page": "83",
      "DOI": "10.1021/bi00167a011",
      "article-title": "Cutinase a lipolytic enzyme with a preformed oxyanion hole",
      "volume": "33",
      "author": "Martinez",
      "year": "1994",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "767",
      "DOI": "10.1038/343767a0",
      "article-title": "A serine protease triad forms the catalytic centre of a triacylglycerol lipase",
      "volume": "343",
      "author": "Brady",
      "year": "1990",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB32",
      "doi-asserted-by": "crossref",
      "first-page": "491",
      "DOI": "10.1038/351491a0",
      "article-title": "A model for interfacial activation in lipases from the structure of a fungal lipase inhibitor complex",
      "volume": "351",
      "author": "Brzozowski",
      "year": "1991",
      "journal-title": "Nature"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB33",
      "doi-asserted-by": "crossref",
      "first-page": "3180",
      "DOI": "10.1021/ja00087a002",
      "article-title": "A structural basis for the chiral preferences of lipases",
      "volume": "116",
      "author": "Cygler",
      "year": "1994",
      "journal-title": "J. Am. Chem. Soc"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB34",
      "doi-asserted-by": "crossref",
      "first-page": "16838",
      "DOI": "10.1021/bi00051a035",
      "article-title": "Crystallographic and molecular-modeling studies of lipase B from Candida antartica reveal a stereospecificity pocket for secondary alcohols",
      "volume": "34",
      "author": "Uppenberg",
      "year": "1995",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB35",
      "series-title": "PCR Technology",
      "first-page": "31",
      "article-title": "Principles and applications for DNA amplification",
      "author": "Higuchi",
      "year": "1992"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB36",
      "doi-asserted-by": "crossref",
      "first-page": "79",
      "DOI": "10.1016/0014-5793(93)81044-Z",
      "article-title": "One-step purification and characterization of human pancreatic lipase expressed in insect cells",
      "volume": "327",
      "author": "Thirstrup",
      "year": "1993",
      "journal-title": "FEBS Lett"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB37",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1111/j.1432-1033.1990.tb15454.x",
      "article-title": "Current approaches to macromolecular crystallization",
      "volume": "189",
      "author": "McPherson",
      "year": "1990",
      "journal-title": "Eur. J. Biochem"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB38",
      "doi-asserted-by": "crossref",
      "first-page": "270",
      "DOI": "10.1016/0022-0248(91)90896-D",
      "article-title": "Application of the streak seeding technique in protein crystallization",
      "volume": "110",
      "author": "Stura",
      "year": "1991",
      "journal-title": "Journal of Crystal Growth"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB39",
      "doi-asserted-by": "crossref",
      "first-page": "362",
      "DOI": "10.1107/S0021889895002706",
      "article-title": "Increasing the size of microcrystals by fine sampling of pH limits",
      "volume": "28",
      "author": "McPherson",
      "year": "1995",
      "journal-title": "J. Appl. Cryst"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB40",
      "doi-asserted-by": "crossref",
      "first-page": "491",
      "DOI": "10.1016/0022-2836(68)90205-2",
      "article-title": "Solvent content of protein crystals",
      "volume": "33",
      "author": "Matthews",
      "year": "1968",
      "journal-title": "J. Mol. Biol"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB41",
      "doi-asserted-by": "crossref",
      "first-page": "982",
      "DOI": "10.1063/1.1143716",
      "article-title": "Bent crystal, bent multilayer optics on a multipole wiggler line for an X-ray diffractometer with an imaging plate detector (biological crystallography)",
      "volume": "63",
      "author": "Fourme",
      "year": "1992",
      "journal-title": "Rev. Sci. Instrum"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB42",
      "series-title": "Proceedings of the CCP4 Study Weekend: Data Collection and Processing",
      "first-page": "56",
      "article-title": "Oscillation Data Reduction Program",
      "author": "Otwinowski",
      "year": "1993"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB43",
      "doi-asserted-by": "crossref",
      "first-page": "392",
      "DOI": "10.1107/S0108767391001071",
      "article-title": "Accurate bond and angle parameters for X-ray protein structure refinement",
      "volume": "47",
      "author": "Engh",
      "year": "1991",
      "journal-title": "Acta Cryst. A"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB44",
      "series-title": "GRASP manual",
      "author": "Nicholls",
      "year": "1993"
    },
    {
      "key": "10.1016/S0969-2126(96)00143-8_BIB45",
      "doi-asserted-by": "crossref",
      "first-page": "103",
      "DOI": "10.1016/0005-2744(68)90248-9",
      "article-title": "Purification and properties of phospholipase A from porcine pancreas",
      "volume": "159",
      "author": "De Haas",
      "year": "1968",
      "journal-title": "Biochim. Biophys. Acta"
    }
  ],
  "container-title": "Structure",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0969212696001438?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S0969212696001438?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        4
      ]
    ],
    "date-time": "2021-07-04T23:10:10Z",
    "timestamp": 1625440210000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S0969212696001438"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1996,
        11
      ]
    ]
  },
  "references-count": 45,
  "journal-issue": {
    "issue": "11",
    "published-print": {
      "date-parts": [
        [
          1996,
          11
        ]
      ]
    }
  },
  "alternative-id": [
    "S0969212696001438"
  ],
  "URL": "http://dx.doi.org/10.1016/S0969-2126(96)00143-8",
  "relation": {},
  "ISSN": [
    "0969-2126"
  ],
  "subject": [],
  "container-title-short": "Structure",
  "published": {
    "date-parts": [
      [
        1996,
        11
      ]
    ]
  }
}