DOI: 10.1016/s0736-5748(01)00018-1. Muscle activity‐resistant acetylcholine receptor accumulation is induced in places of former motor endplates in ectopically innervated regenerating rat muscles

Muscle activity‐resistant acetylcholine receptor accumulation is induced in places of former motor endplates in ectopically innervated regenerating rat muscles

10.1016/s0736-5748(01)00018-1

Crossref journal-article

Wiley

International Journal of Developmental Neuroscience (311)

Abstract

AbstractExpression of acetylcholine receptors (AChRs) in the extrajunctional muscle regions, but not in the neuromuscular junctions, is repressed by propagated electric activity in muscle fibers. During regeneration, subsynaptic‐like specializations accumulating AChRs are induced in new myotubes by agrin attached to the synaptic basal lamina at the places of former motor endplates even in the absence of innervation. We examined whether AChRs still accumulated at these places when the regenerating muscles were ectopically innervated and the former synaptic places became extrajunctional. Rat soleus muscles were injured by bupivacaine and ischemia to produce complete myofiber degeneration. The soleus muscle nerve was permanently severed and the muscle was ectopically innervated by the peroneal nerve a few millimeters away from the former junctional region. After 4 weeks of regeneration, the muscles contracted upon nerve stimulation, showed little atrophy and the cross‐section areas of their fibers were completely above the range in non‐innervated regenerating muscles, indicating successful innervation. Subsynaptic‐like specializations in the former junctional region still accumulated AChRs (and acetylcholinesterase) although no motor nerve endings were observed in their vicinity and the cross‐section area of their fibers clearly demonstrated that they were ectopically innervated. We conclude that the expression of AChRs at the places of the former neuromuscular junctions in the ectopically innervated regenerated soleus muscles is activity‐independent.

Bibliography

GaÅ¡perÅ¡iÄ, R., Koritnik, B., ErÅ¾en, I., & Sketelj, J. (2001). Muscle activityâresistant acetylcholine receptor accumulation is induced in places of former motor endplates in ectopically innervated regenerating rat muscles. International Journal of Developmental Neuroscience, 19(3), 339â346. Portico.

Authors 4

Rok Gašperšič (first)
Blaž Koritnik (additional)
Ida Eržen (additional)
Janez Sketelj (additional)

References 39 Referenced 15

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10.1242/dev.116.1.41 / Development / Synapse‐specific expression of acetylcholine receptor genes and their products at original synaptic sites in rat soleus muscle fibres regenerating in the absence of innervation by Brenner H.R. (1992)
{'key': 'e_1_2_7_7_1', 'first-page': '387', 'article-title': 'A review of muscle transplantation in mammals', 'volume': '27', 'author': 'Carlson B.M.', 'year': '1978', 'journal-title': 'Physiol. Bohemoslov.'} / Physiol. Bohemoslov. / A review of muscle transplantation in mammals by Carlson B.M. (1978)
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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 5:54 p.m.)
Deposited	1 year, 11 months ago (Sept. 6, 2023, 4:56 p.m.)
Indexed	2 months ago (July 1, 2025, 9:48 a.m.)
Issued	24 years, 4 months ago (April 30, 2001)
Published	24 years, 4 months ago (April 30, 2001)
Published Online	24 years, 4 months ago (April 30, 2001)
Published Print	24 years, 3 months ago (June 1, 2001)

Funders 1

Ministry of Science and Technology of the Republic of Slovenia

BibTeX

@article{Ga_per_i__2001, title={Muscle activity‐resistant acetylcholine receptor accumulation is induced in places of former motor endplates in ectopically innervated regenerating rat muscles}, volume={19}, ISSN={1873-474X}, url={http://dx.doi.org/10.1016/s0736-5748(01)00018-1}, DOI={10.1016/s0736-5748(01)00018-1}, number={3}, journal={International Journal of Developmental Neuroscience}, publisher={Wiley}, author={Gašperšič, Rok and Koritnik, Blaž and Eržen, Ida and Sketelj, Janez}, year={2001}, month=apr, pages={339–346} }

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